LOCUS       sequence-215583-        1820 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BTK Type 2 promoter for golden gate assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Leonard SP, Perutka J, Powell JE, Geng P, Richhart DD, Byrom M, Kar 
            S, Davies BW, Ellington AD, Moran NA, Barrick JE
  TITLE     Genetic Engineering of Bee Gut Microbiome Bacteria with a Toolkit 
            for Modular Assembly of Broad-Host-Range Plasmids.
  JOURNAL   ACS Synth Biol. 2018 May 18;7(5):1279-1290. doi: 
            10.1021/acssynbio.7b00399. Epub 2018 Apr 13.
  PUBMED    29608282
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.7b00399"; journalName: "ACS Synth Biol"; date: 
            "2018-05-18- 18"; volume: "7"; issue: "5"; pages: "1279-1290"
COMMENT     Sequence Label: pBTK130
FEATURES             Location/Qualifiers
     source          1..1820
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(44..632)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(124..143)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             814..1473
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(880..899)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1544..1578
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     protein_bind    1616..1640
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(1644..1663)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1645..1663)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
       61 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
      121 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
      181 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
      241 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
      301 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
      361 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
      421 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
      481 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
      541 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
      601 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
      661 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgatt gatcgggcac
      721 gtaagaggtt ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag
      781 ttatcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc
      841 accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct
      901 caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag
      961 aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct
     1021 catccggaat ttcgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac
     1081 ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac
     1141 cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa
     1201 aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtttc agccaatccc
     1261 tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc
     1321 gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt
     1381 caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa
     1441 cagtactgcg atgagtggca gggcggggcg taatttgata tcgagctcgc ttggactcct
     1501 gttgatagat ccagtaatga cctcagaact ccatctggat ttgttcagaa cgctcggttg
     1561 ccgccgggcg ttttttattg gtctggtctc acatatatct cctctgtttc tgtatggaat
     1621 tgttatccgc tcacaattcc tctccctata gtgagtcgta ttaatttgac agtcattcat
     1681 ctttctgccc ctccaaaagc aaaaacccgc cgaagcgggt ttttacgtaa atcaggtgaa
     1741 actgaccgac gtttgagacc gactacggtt atccacagaa tcaggggata acgcaggaaa
     1801 gaacatgtga gcaaaaggcc
//
