LOCUS       sequence-215588-        5998 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses full length PARP1 with K to Q mutations in 
            automodification domain in Sf9 (insect) cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5998)
  AUTHORS   Liszczak G, Diehl KL, Dann GP, Muir TW
  TITLE     Acetylation blocks DNA damage-induced chromatin ADP-ribosylation.
  JOURNAL   Nat Chem Biol. 2018 Jul 16. pii: 10.1038/s41589-018-0097-1. doi: 
            10.1038/s41589-018-0097-1.
  PUBMED    30013063
REFERENCE   2  (bases 1 to 5998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2018 Jul 16. pii: 10.1038/s41589-018-0097-1. doi: 
            10.1038/s41589-018-0097-1."
COMMENT     Sequence Label: pACEBac1-PARP1-Flag-His-7xKQ
FEATURES             Location/Qualifiers
     source          1..5998
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  135..358
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(425..958)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1147..1152,1153..1169,1170..1175))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(1246..1279)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        1356..1447
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     1372..1389
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             4547..4570
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4610..4627
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4800..4934
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4837..4856)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4891..4910
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  5055..5220
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      5410..5998
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5899..5918
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattgactt gggtcgctct tcctgtggat gcgcagatgc
      121 cctgcgtaag cgggtgtggg cggacaataa agtcttaaac tgaacaaaat agatctaaac
      181 tatgacaata aagtcttaaa ctagacagaa tagttgtaaa ctgaaatcag tccagttatg
      241 ctgtgaaaaa gcatactgga cttttgttat ggctaaagca aactcttcat tttctgaagt
      301 gcaaattgcc cgtcgtatta aagaggggcg tggccaaggg catgtaaaga ctatattcgc
      361 ggcgttgtga caatttaccg aacaactccg cggccgggaa gccgatctcg gcttgaacga
      421 attgttaggt ggcggtactt gggtcgatat caaagtgcat cacttcttcc cgtatgccca
      481 actttgtata gagagccact gcgggatcgt caccgtaatc tgcttgcacg tagatcacat
      541 aagcaccaag cgcgttggcc tcatgcttga ggagattgat gagcgcggtg gcaatgccct
      601 gcctccggtg ctcgccggag actgcgagat catagatata gatctcacta cgcggctgct
      661 caaacttggg cagaacgtaa gccgcgagag cgccaacaac cgcttcttgg tcgaaggcag
      721 caagcgcgat gaatgtctta ctacggagca agttcccgag gtaatcggag tccggctgat
      781 gttgggagta ggtggctacg tctccgaact cacgaccgaa aagatcaaga gcagcccgca
      841 tggatttgac ttggtcaggg ccgagcctac atgtgcgaat gatgcccata cttgagccac
      901 ctaactttgt tttagggcga ctgccctgct gcgtaacatc gttgctgctg cgtaacatcg
      961 ttgctgctcc ataacatcaa acatcgaccc acggcgtaac gcgcttgctg cttggatgcc
     1021 cgaggcatag actgtacaaa aaaacagtca taacaagcca tgaaaaccgc cactgcgccg
     1081 ttaccaccgc tgcgttcggt caaggttctg gaccagttgc gtgagcgcat acgctacttg
     1141 cattacagtt tacgaaccga acaggcttat gtcaactggg ttcgtgcctt catccgtttc
     1201 cacggtgtgc gtcacccggc aaccttgggc agcagcgaag tcgccataac ttcgtatagc
     1261 atacattata cgaagttatc tgtaactata acggtcctaa ggtagcgagt ttaaacacta
     1321 gtatcgattc gcgacctact ccggaatatt aatagatcat ggagataatt aaaatgataa
     1381 ccatctcgca aataaataag tattttactg ttttcgtaac agttttgtaa taaaaaaacc
     1441 tataaatatt ccggattatt cataccgtcc caccatcggg cgcggatcta tggcggagtc
     1501 ttcggataag ctctatcgag tcgagtacgc caagagcggg cgcgcctctt gcaagaaatg
     1561 cagcgagagc atccccaagg actcgctccg gatggccatc atggtgcagt cgcccatgtt
     1621 tgatggaaaa gtcccacact ggtaccactt ctcctgcttc tggaaggtgg gccactccat
     1681 ccggcaccct gacgttgagg tggatgggtt ctctgagctt cggtgggatg atcagcagaa
     1741 agtcaagaag acagcggaag ctggaggagt gacaggcaaa ggccaggatg gaattggtag
     1801 caaggcagag aagactctgg gtgactttgc agcagagtat gccaagtcca acagaagtac
     1861 gtgcaagggg tgtatggaga agatagaaaa gggccaggtg cgcctgtcca agaagatggt
     1921 ggacccggag aagccacagc taggcatgat tgaccgctgg taccatccag gctgctttgt
     1981 caagaacagg gaggagctgg gtttccggcc cgagtacagt gcgagtcagc tcaagggctt
     2041 cagcctcctt gctacagagg ataaagaagc cctgaagaag cagctcccag gagtcaagag
     2101 tgaaggaaag agaaaaggcg atgaggtgga tggagtggat gaagtggcga agaagaaatc
     2161 taaaaaagaa aaagacaagg atagtaagct tgaaaaagcc ctaaaggctc agaacgacct
     2221 gatctggaac atcaaggacg agctaaagaa agtgtgttca actaatgacc tgaaggagct
     2281 actcatcttc aacaagcagc aagtgccttc tggggagtcg gcgatcttgg accgagtagc
     2341 cgatggcatg gtgttcggtg ccctccttcc ctgcgaggaa tgctcgggtc agctggtctt
     2401 caagagcgat gcctattact gcactgggga cgtcactgcc tggaccaagt gtatggtcaa
     2461 gacacagaca cccaaccgga aggagtgggt aaccccaaag gaattccgag aaatctctta
     2521 cctcaagaaa ttgaaggtta aaaaacagga ccgtatattc cccccagaaa ccagcgcctc
     2581 cgtggcggcc acgcctccgc cctccacagc ctcggctcct gctgctgtga actcctctgc
     2641 ttcagcagat aagccattat ccaacatgaa gatcctgact ctcgggaagc tgtcccggaa
     2701 caaggatgaa gtgaaggcca tgattgagaa actcgggggg aagttgacgg ggacggccaa
     2761 caaggcttcc ctgtgcatca gcaccaaaaa ggaggtggaa aagatgaata agaagatgga
     2821 ggaagtaaag gaagccaaca tccgagttgt gtctgaggac ttcctccagg acgtctccgc
     2881 ctccaccaag agccttcagg agttgttctt agcgcacatc ttgtcccctt ggggggcaga
     2941 ggtgaaggca gagcctgttg aagttgtggc cccaagaggg cagtcagggg ctgcgctctc
     3001 ccagcagagc cagggccagg tcaaggagga aggtatcaac cagtctgaac agagaatgca
     3061 gttaactctt aaaggaggag cagctgtgga tcctgattct ggactggaac actctgcgca
     3121 tgtcctggag aaaggtggga aggtcttcag tgccaccctt ggcctggtgg acatcgttaa
     3181 aggaaccaac tcctactaca agctgcagct tctggaggac gacaaggaaa acaggtattg
     3241 gatattcagg tcctggggcc gtgtgggtac ggtgatcggt agcaacaaac tggaacagat
     3301 gccgtccaag gaggatgcca ttgagcactt catgaaatta tatgaagaaa aaaccgggaa
     3361 cgcttggcac tccaaaaatt tcacgaagta tcccaaaaag ttctaccccc tggagattga
     3421 ctatggccag gatgaagagg cagtgaagaa gctgacagta aatcctggca ccaagtccaa
     3481 gctccccaag ccagttcagg acctcatcaa gatgatcttt gatgtggaaa gtatgaagaa
     3541 agccatggtg gagtatgaga tcgaccttca gaagatgccc ttggggaagc tgagcaaaag
     3601 gcagatccag gccgcatact ccatcctcag tgaggtccag caggcggtgt ctcagggcag
     3661 cagcgactct cagatcctgg atctctcaaa tcgcttttac accctgatcc cccacgactt
     3721 tgggatgaag aagcctccgc tcctgaacaa tgcagacagt gtgcaggcca aggcggaaat
     3781 gcttgacaac ctgctggaca tcgaggtggc ctacagtctg ctcaggggag ggtctgatga
     3841 tagcagcaag gatcccatcg atgtcaacta tgagaagctc aaaactgaca ttaaggtggt
     3901 tgacagagat tctgaagaag ccgagatcat caggaagtat gttaagaaca ctcatgcaac
     3961 cacacacaat gcgtatgact tggaagtcat cgatatcttt aagatagagc gtgaaggcga
     4021 atgccagcgt tacaagccct ttaagcagct tcataaccga agattgctgt ggcacgggtc
     4081 caggaccacc aactttgctg ggatcctgtc ccagggtctt cggatagccc cgcctgaagc
     4141 gcccgtgaca ggctacatgt ttggtaaagg gatctatttc gctgacatgg tctccaagag
     4201 tgccaactac tgccatacgt ctcagggaga cccaataggc ttaatcctgt tgggagaagt
     4261 tgcccttgga aacatgtatg aactgaagca cgcttcacat atcagcaagt tacccaaggg
     4321 caagcacagt gtcaaaggtt tgggcaaaac tacccctgat ccttcagcta acattagtct
     4381 ggatggtgta gacgttcctc ttgggaccgg gatttcatct ggtgtgaatg acacctctct
     4441 actatataac gagtacattg tctatgatat tgctcaggta aatctgaagt atctgctgaa
     4501 actgaaattc aattttaaga cctccctgtg gggcggatct ggcggtgact ataaagatga
     4561 tgatgataaa ggtggtagtg gagtcgacga gctcactgga ggtagcggac accatcacca
     4621 tcaccactga tgtcgcggcc gctttcgaat ctagagcctg cagtctcgac aagcttgtcg
     4681 agaagtacta gaggatcata atcagccata ccacatttgt agaggtttta cttgctttaa
     4741 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
     4801 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     4861 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     4921 atcatgtctg gatctgatca ctgcttgagc ctagaagatc cggctgctaa caaagcccga
     4981 aaggaagctg agttggctgc tgccaccgct gagcaataac tatcataacc cctagggtat
     5041 acccatctaa ttggaaccag ataagtgaaa tctagttcca aactattttg tcatttttaa
     5101 ttttcgtatt agcttacgac gctacaccca gttcccatct attttgtcac tcttccctaa
     5161 ataatcctta aaaactccat ttccacccct cccagttccc aactattttg tccgcccaca
     5221 accggttgac ttgggtcaac tgtcagacca agtttactca tatatacttt agattgattt
     5281 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     5341 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     5401 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     5461 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     5521 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     5581 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     5641 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     5701 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     5761 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     5821 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     5881 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     5941 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaa
//
