LOCUS       sequence-215590-        8816 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet-inducible shRNA targeting GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8816)
  AUTHORS   Ilic N, Birsoy K, Aguirre AJ, Kory N, Pacold ME, Singh S, Moody SE, 
            DeAngelo JD, Spardy NA, Freinkman E, Weir BA, Tsherniak A, Cowley 
            GS, Root DE, Asara JM, Vazquez F, Widlund HR, Sabatini DM, Hahn WC
  TITLE     PIK3CA mutant tumors depend on oxoglutarate dehydrogenase.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Apr 25;114(17):E3434-E3443. doi: 
            10.1073/pnas.1617922114. Epub 2017 Apr 10.
  PUBMED    28396387
REFERENCE   2  (bases 1 to 8816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1617922114"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2017-04-25- 25"; volume: "114"; issue: "17"; pages: 
            "E3434-E3443"
COMMENT     Sequence Label: pLKO-puro-tetON-shGFP
FEATURES             Location/Qualifiers
     source          1..8816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      19..607
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     508..527
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     761..778
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    895..916
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(931..936,937..954,955..961)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    969..985
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     974..996
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     993..1009
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     993..1009
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1028..1048
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1030..1048
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1076..1302
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1303..1483
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1530..1655
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2148..2381
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2566..2610
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2899..2993
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     primer_bind     2910..2929
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    2947..2965
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2975..2993
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    3094..3211
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3260..3770
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3689..3707
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     intron          3779..4351
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     4359..4378
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        4406..4424
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4435..5055
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(4508..4527)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     misc_feature    5090..5663
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5257..5274)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5484..5503
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5683..6282
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5683..5702)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6179..6199
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6410..6643
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6715..6836
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6752..6771)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6806..6825
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6876..7011
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(7032..7050)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7060..7077)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7060..7076)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7069..7091)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7218..7673
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7305..7324)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7515..7536
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7699..7803
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7804..7872,7873..8664)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8022..8041)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
       61 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
      121 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
      181 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg atctgctaat
      241 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
      301 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
      361 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
      421 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
      481 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
      541 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
      601 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
      661 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
      721 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
      781 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
      841 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
      901 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
      961 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
     1021 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag
     1081 tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta
     1141 caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc
     1201 ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat
     1261 tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac
     1321 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     1381 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     1441 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
     1501 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
     1561 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
     1621 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
     1681 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
     1741 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
     1801 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
     1861 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
     1921 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
     1981 ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
     2041 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     2101 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
     2161 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
     2221 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
     2281 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
     2341 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
     2401 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
     2461 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
     2521 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
     2581 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
     2641 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     2701 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
     2761 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag
     2821 agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg atcacgagac
     2881 tagcctcgag cggccgcaat atttgcatgt cgctatgtgt tctgggaaat caccataaac
     2941 gtgaaatccc tatcagtgat agagacttat aagttcccta tcagtgatag agacaccggc
     3001 aacagccaca acgtctatat ctcgagatat agacgttgtg gctgttgttt ttgaattctc
     3061 gacctcgaga caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg
     3121 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
     3181 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc
     3241 actttggccg cggctcgagg gggttggggt tgcgcctttt ccaaggcagc cctgggtttg
     3301 cgcagggacg cggctgctct gggcgtggtt ccgggaaacg cagcggcgcc gaccctgggt
     3361 ctcgcacatt cttcacgtcc gttcgcagcg tcacccggat cttcgccgct acccttgtgg
     3421 gccccccggc gacgcttcct gctccgcccc taagtcggga aggttccttg cggttcgcgg
     3481 cgtgccggac gtgacaaacg gaagccgcac gtctcactag taccctcgca gacggacagc
     3541 gccagggagc aatggcagcg cgccgaccgc gatgggctgt ggccaatagc ggctgctcag
     3601 cagggcgcgc cgagagcagc ggccgggaag gggcggtgcg ggaggcgggg tgtggggcgg
     3661 tagtgtgggc cctgttcctg cccgcgcggt gttccgcatt ctgcaagcct ccggagcgca
     3721 cgtcggcagt cggctccctc gttgaccgaa tcaccgacct ctctccccag ggggatctgt
     3781 gagtttgggg acccttgatt gttctttctt tttcgctatt gtaaaattca tgttatatgg
     3841 agggggcaaa gttttcaggg tgttgtttag aatgggaaga tgtcccttgt atcaccatgg
     3901 accctcatga taattttgtt tctttcactt tctactctgt tgacaaccat tgtctcctct
     3961 tattttcttt tcattttctg taactttttc gttaaacttt agcttgcatt tgtaacgaat
     4021 ttttaaattc acttttgttt atttgtcaga ttgtaagtac tttctctaat cacttttttt
     4081 tcaaggcaat cagggtatat tatattgtac ttcagcacag ttttagagaa caattgttat
     4141 aattaaatga taaggtagaa tatttctgca tataaattct ggctggcgtg gaaatattct
     4201 tattggtaga aacaactaca tcctggtcat catcctgcct ttctctttat ggttacaatg
     4261 atatacactg tttgagatga ggataaaata ctctgagtcc aaaccgggcc cctctgctaa
     4321 ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct
     4381 gtctcatcat tttggcaaag aattgtaata cgactcacta tagggcgaat tgatatgtct
     4441 agattagata aaagtaaagt gattaacagc gcattagagc tgcttaatga ggtcggaatc
     4501 gaaggtttaa caacccgtaa actcgcccag aagctaggtg tagagcagcc tacattgtat
     4561 tggcatgtaa aaaataagcg ggctttgctc gacgccttag ccattgagat gttagatagg
     4621 caccatactc acttttgccc tttagaaggg gaaagctggc aagatttttt acgtaataac
     4681 gctaaaagtt ttagatgtgc tttactaagt catcgcgatg gagcaaaagt acatttaggt
     4741 acacggccta cagaaaaaca gtatgaaact ctcgaaaatc aattagcctt tttatgccaa
     4801 caaggttttt cactagagaa tgcattatat gcactcagcg ctgtggggca ttttacttta
     4861 ggttgcgtat tggaagatca agagcatcaa gtcgctaaag aagaaaggga aacacctact
     4921 actgatagta tgccgccatt attacgacaa gctatcgaat tatttgatca ccaaggtgca
     4981 gagccagcct tcttattcgg ccttgaattg atcatatgcg gattagaaaa acaacttaaa
     5041 tgtgaaagtg ggtccgcgta cagcggctcc cgggagttct agggatctgc ccctctccct
     5101 cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct
     5161 atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc
     5221 ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc
     5281 tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg
     5341 tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa
     5401 agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt
     5461 ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg
     5521 atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta
     5581 catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt
     5641 tcctttgaaa aacacgatga taaggatcca ccggagctta ccatgaccga gtacaagccc
     5701 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
     5761 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
     5821 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     5881 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     5941 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     6001 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     6061 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     6121 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     6181 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     6241 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gacgcccgcc ccacgacccg
     6301 cagcgcccga ccgaaaggag cgcacgaccc catgcatcgg tacctttaag accaatgact
     6361 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     6421 attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac
     6481 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     6541 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     6601 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat
     6661 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg
     6721 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     6781 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     6841 gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     6901 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     6961 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt
     7021 acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg
     7081 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     7141 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     7201 cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     7261 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     7321 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     7381 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     7441 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     7501 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     7561 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     7621 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc
     7681 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     7741 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     7801 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     7861 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     7921 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     7981 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     8041 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     8101 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     8161 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     8221 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     8281 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     8341 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     8401 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     8461 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     8521 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     8581 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     8641 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     8701 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     8761 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtaga
//
