LOCUS       sequence-215605-        3051 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ""Vector AA"".
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Jenni S, Harrison SC
  TITLE     Structure of the DASH/Dam1 complex shows its role at the yeast 
            kinetochore-microtubule interface.
  JOURNAL   Science. 2018 May 4;360(6388):552-558. doi: 10.1126/science.aar6436.
  PUBMED    29724956
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2018-05-4- 4"; volume: "360"; issue: 
            "6388"; pages: "552-558"
COMMENT     Sequence Label: peAA.11
FEATURES             Location/Qualifiers
     source          1..3051
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      5..593
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     494..513
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     747..764
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    779..918
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(844..863)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1004..1026
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     protein_bind    1097..1116
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     primer_bind     1125..1143
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     protein_bind    1246..1265
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    complement(1316..1349)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    1736..1763
                     /gene="telL"
                     /label=telL
                     /note="telomeric sequence from coliphage N15"
     misc_feature    1764..1791
                     /gene="telR"
                     /label=telR
                     /note="telomeric sequence from coliphage N15"
     protein_bind    complement(1949..1982)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        2030..2121
                     /gene="bla"
                     /label=AmpR promoter
     terminator      2986..3025
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
       61 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
      121 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
      181 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
      241 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
      301 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
      361 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
      421 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
      481 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
      541 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gaacctatgg aaaaacgcca
      601 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
      661 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
      721 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
      781 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcaat ggtgcactct
      841 cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt
      901 gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
      961 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     1021 cagaggtttt caccgtcatc accgaaacgc gcgaggcagg gggaattccc aattagatgg
     1081 gtttaaaccg ccggcgaatt gtgagcgctc acaattggcg cgccgctagt tattgctcag
     1141 cggtggcagc agcctctcga gaggccttgt actattagaa gtatatttct agaatcgata
     1201 cctaggtcgc taccttagga ccgttatagt tatgcatact cgagtaattg tgagcgctca
     1261 caattggccg gccggtgcca acacctacat ctcctctacg cggccgcggt accccataac
     1321 ttcgtatagc atacattata cgaagttatc catgggaagc ttaaatgttt tacatggtcc
     1381 taatcgggtg caccgctcgg gcggaccaaa acccatgacc actaaaatag agctgagcct
     1441 agtcgcgtga tttgacttaa gttaattaag gccggccatt tcggtctgta cgactatgga
     1501 tcggcaattt gctagacgat ctggcacaga aagacacaga cgaaggactc actatggaat
     1561 ggcggcttcg gccgtcaatg gcgcgccgat ggatcccagc ggaagtagga agcgatgtgg
     1621 cctgaggaat acgtctgtca ttgcgtttag ataaggcatc ccgctggaaa gggaaccagt
     1681 actttgtaaa ggagctagag gttctctcgc cggcgtcact cggagaatgg caatgtatca
     1741 gcacacaata gtccattata cgcgcgtata atgggcaatt gtgtgctgat acgccggcgt
     1801 tttaattaaa tgctctgtgg ttcgtgcggt tcttaaaagc aaacgacgag cgaactggtg
     1861 cgtgggcaaa aggggaccct catttctaag tacgatcagg tggactacga ttgcatttta
     1921 tgggttccct gcaggaattc ctcgagccat aacttcgtat agcatacatt atacgaagtt
     1981 atctgactag tagcccgcct aatgagcggg ctttttttta attcccctat ttgtttattt
     2041 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     2101 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     2161 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctcgtgaa agtaaaagac
     2221 gcagaggacc aattgggggc acgagtggga tacatagaac tggacttgaa tagcggtaaa
     2281 atccttgaga gttttcgccc tgaagagcgt tttccaatga tgagcacttt caaagttctg
     2341 ctatgtggag cagtattatc ccgtgtagat gcggggcaag agcaactcgg acgacgaata
     2401 cactattcgc agaatgactt ggttgaatac tccccagtga cagaaaagca ccttacggac
     2461 ggaatgacgg taagagaatt atgtagtgcc gccataacga tgagtgataa cactgcggcg
     2521 aacttacttc tgacaaccat cggtggaccg aaggaattaa ccgctttttt gcacaatatg
     2581 ggagaccatg taactcgcct tgaccgttgg gaaccagaac tgaatgaagc cataccaaac
     2641 gacgagcgag acaccacaat gcctgcggca atggcaacaa cattacgcaa actattaact
     2701 ggcgaactac ttactctggc ttcacggcaa caattaatag actggcttga agcggataaa
     2761 gttgcaggac cactactgcg ttcggcactt cctgctggct ggtttattgc tgataaatct
     2821 ggggcaggag agcgtggttc acggggtatc attgccgcac ttggaccaga tggtaagcct
     2881 tcccgtatcg tagttatcta cacgacgggt agtcaggcaa ctatggacga acgaaataga
     2941 cagattgctg aaataggggc ttcactgatt aagcattggt aaaccgatac aattaaaggc
     3001 tccttttgga gccttttttt ttggacggac cggtagaaaa gatcaaagga t
//
