LOCUS       sequence-215614-        9522 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Adenovirus overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9522)
  AUTHORS   Zhou Q, Gallagher R, Ufret-Vincenty R, Li X, Olson EN, Wang S
  TITLE     Regulation of angiogenesis and choroidal neovascularization by 
            members of microRNA-23~27~24 clusters.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 May 17;108(20):8287-92. doi: 
            10.1073/pnas.1105254108. Epub 2011 May 2.
  PUBMED    21536891
REFERENCE   2  (bases 1 to 9522)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1105254108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-05-17- 17"; volume: "108"; issue: "20"; pages: "8287-92"
COMMENT     Sequence Label: Adeno-miR-23b
FEATURES             Location/Qualifiers
     source          1..9522
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    683..804
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(720..739)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     774..793
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   3995..4097
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     primer_bind     complement(4186..4203)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4357..4945)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             5127..5786
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(5193..5212)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      5857..5891
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     terminator      complement(5912..5959)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      complement(6141..6729)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             7561..8355
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7561..8355
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7615..7634)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8225..8244
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      complement(8403..8783)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8465..8486)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8677..8696
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   8821..8923
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     misc_signal     9011..9161
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     enhancer        9219..9522
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gagatctggt
      241 accgctagct taacaggcgt gaaggcggca gtgtcgccgg cgtccttccg cggaagccca
      301 gtgtgtgcag acagcacggg gtggcgctgc tctcaggtgc tctggctgct tgggttcctg
      361 gcatgctgat ttgtgactta agattaaaat cacattgcca gggattacca cgcaaccacg
      421 accttggctg ctcctccaga aaccgtggtc gcgctcactg cagattggag aacaggtgca
      481 tctcgtagct cttctttgga aacaaaagaa gccaccagcg gccgctcgag cctaagcttc
      541 tagataagat atccgatcca ccggatctag ataactgatc ataatcagcc ataccacatt
      601 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
      661 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
      721 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
      781 gtccaaactc atcaatgtat cttaacgcgg atctgggcgt ggttaagggt gggaaagaat
      841 atataaggtg ggggtcttat gtagttttgt atctgttttg cagcagccgc cgccgccatg
      901 agcaccaact cgtttgatgg aagcattgtg agctcatatt tgacaacgcg catgccccca
      961 tgggccgggg tgcgtcagaa tgtgatgggc tccagcattg atggtcgccc cgtcctgccc
     1021 gcaaactcta ctaccttgac ctacgagacc gtgtctggaa cgccgttgga gactgcagcc
     1081 tccgccgccg cttcagccgc tgcagccacc gcccgcggga ttgtgactga ctttgctttc
     1141 ctgagcccgc ttgcaagcag tgcagcttcc cgttcatccg cccgcgatga caagttgacg
     1201 gctcttttgg cacaattgga ttctttgacc cgggaactta atgtcgtttc tcagcagctg
     1261 ttggatctgc gccagcaggt ttctgccctg aaggcttcct cccctcccaa tgcggtttaa
     1321 aacataaata aaaaaccaga ctctgtttgg atttggatca agcaagtgtc ttgctgtctt
     1381 tatttagggg ttttgcgcgc gcggtaggcc cgggaccagc ggtctcggtc gttgagggtc
     1441 ctgtgtattt tttccaggac gtggtaaagg tgactctgga tgttcagata catgggcata
     1501 agcccgtctc tggggtggag gtagcaccac tgcagagctt catgctgcgg ggtggtgttg
     1561 tagatgatcc agtcgtagca ggagcgctgg gcgtggtgcc taaaaatgtc tttcagtagc
     1621 aagctgattg ccaggggcag gcccttggtg taagtgttta caaagcggtt aagctgggat
     1681 gggtgcatac gtggggatat gagatgcatc ttggactgta tttttaggtt ggctatgttc
     1741 ccagccatat ccctccgggg attcatgttg tgcagaacca ccagcacagt gtatccggtg
     1801 cacttgggaa atttgtcatg tagcttagaa ggaaatgcgt ggaagaactt ggagacgccc
     1861 ttgtgacctc caagattttc catgcattcg tccataatga tggcaatggg cccacgggcg
     1921 gcggcctggg cgaagatatt tctgggatca ctaacgtcat agttgtgttc caggatgaga
     1981 tcgtcatagg ccatttttac aaagcgcggg cggagggtgc cagactgcgg tataatggtt
     2041 ccatccggcc caggggcgta gttaccctca cagatttgca tttcccacgc tttgagttca
     2101 gatgggggga tcatgtctac ctgcggggcg atgaagaaaa cggtttccgg ggtaggggag
     2161 atcagctggg aagaaagcag gttcctgagc agctgcgact taccgcagcc ggtgggcccg
     2221 taaatcacac ctattaccgg ctgcaactgg tagttaagag agctgcagct gccgtcatcc
     2281 ctgagcaggg gggccacttc gttaagcatg tccctgactc gcatgttttc cctgaccaaa
     2341 tccgccagaa ggcgctcgcc gcccagcgat agcagttctt gcaaggaagc aaagtttttc
     2401 aacggtttga gaccgtccgc cgtaggcatg cttttgagcg tttgaccaag cagttccagg
     2461 cggtcccaca gctcggtcac ctgctctacg gcatctcgat ccagcatatc tcctcgtttc
     2521 gcgggttggg gcggctttcg ctgtacggca gtagtcggtg ctcgtccaga cgggccaggg
     2581 tcatgtcttt ccacgggcgc agggtcctcg tcagcgtagt ctgggtcacg gtgaaggggt
     2641 gcgctccggg ctgcgcgctg gccagggtgc gcttgaggct ggtcctgctg gtgctgaagc
     2701 gctgccggtc ttcgccctgc gcgtcggcca ggtagcattt gaccatggtg tcatagtcca
     2761 gcccctccgc ggcgtggccc ttggcgcgca gcttgccctt ggaggaggcg ccgcacgagg
     2821 ggcagtgcag acttttgagg gcgtagagct tgggcgcgag aaataccgat tccggggagt
     2881 aggcatccgc gccgcaggcc ccgcagacgg tctcgcattc cacgagccag gtgagctctg
     2941 gccgttcggg gtcaaaaacc aggtttcccc catgcttttt gatgcgtttc ttacctctgg
     3001 tttccatgag ccggtgtcca cgctcggtga cgaaaaggct gtccgtgtcc ccgtatacag
     3061 acttgagagg gagtttaaac gaattcaata gcttgttgca tgggcggcga tataaaatgc
     3121 aaggtgctgc tcaaaaaatc aggcaaagcc tcgcgcaaaa aagaaagcac atcgtagtca
     3181 tgctcatgca gataaaggca ggtaagctcc ggaaccacca cagaaaaaga caccattttt
     3241 ctctcaaaca tgtctgcggg tttctgcata aacacaaaat aaaataacaa aaaaacattt
     3301 aaacattaga agcctgtctt acaacaggaa aaacaaccct tataagcata agacggacta
     3361 cggccatgcc ggcgtgaccg taaaaaaact ggtcaccgtg attaaaaagc accaccgaca
     3421 gctcctcggt catgtccgga gtcataatgt aagactcggt aaacacatca ggttgattca
     3481 catcggtcag tgctaaaaag cgaccgaaat agcccggggg aatacatacc cgcaggcgta
     3541 gagacaacat tacagccccc ataggaggta taacaaaatt aataggagag aaaaacacat
     3601 aaacacctga aaaaccctcc tgcctaggca aaatagcacc ctcccgctcc agaacaacat
     3661 acagcgcttc cacagcggca gccataacag tcagccttac cagtaaaaaa gaaaacctat
     3721 taaaaaaaca ccactcgaca cggcaccagc tcaatcagtc acagtgtaaa aaagggccaa
     3781 gtgcagagcg agtatatata ggactaaaaa atgacgtaac ggttaaagtc cacaaaaaac
     3841 acccagaaaa ccgcacgcga acctacgccc agaaacgaaa gccaaaaaac ccacaacttc
     3901 ctcaaatcgt cacttccgtt ttcccacgtt acgtcacttc ccattttaag aaaactacaa
     3961 ttcccaacac atacaagtta ctccgcccta aaacctacgt cacccgcccc gttcccacgc
     4021 cccgcgccac gtcacaaact ccaccccctc attatcatat tggcttcaat ccaaaataag
     4081 gtatattatt gatgatgtta attaacatgc atggatccat atgcggtgtg aaataccgca
     4141 cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc
     4201 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     4261 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     4321 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     4381 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     4441 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     4501 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     4561 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     4621 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     4681 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     4741 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     4801 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     4861 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     4921 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     4981 tcagtggaac gaaaactcac gttaagggat tttggtcatg attgatcggg cacgtaagag
     5041 gttccaactt tcaccataat gaaataagat cactaccggg cgtatttttt gagttatcga
     5101 gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg
     5161 atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta
     5221 cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata
     5281 agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg
     5341 aatttcgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt
     5401 acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg
     5461 atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg
     5521 cctatttccc taaagggttt attgagaata tgtttttcgt ttcagccaat ccctgggtga
     5581 gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca
     5641 ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc
     5701 atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact
     5761 gcgatgagtg gcagggcggg gcgtaatttg atatcgagct cgcttggact cctgttgata
     5821 gatccagtaa tgacctcaga actccatctg gatttgttca gaacgctcgg ttgccgccgg
     5881 gcgtttttta ttggtctggt ctcacagcca gcaaaaaacc cctcaagacc cgtttagagg
     5941 ccccaagggg ttatgctagt taggattgag accgactacg gttatccaca gaatcagggg
     6001 ataacgcagg aaagaacatg tgtatgcgtt tgagaccgac tacggttatc cacagaatca
     6061 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     6121 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     6181 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     6241 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     6301 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     6361 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     6421 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     6481 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     6541 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     6601 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     6661 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     6721 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     6781 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     6841 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     6901 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     6961 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     7021 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     7081 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     7141 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     7201 gttgttgcca ttgctgcagc catgagatta tcaaaaagga tcttcaccta gatccttttc
     7261 acgtagaaag ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag ctactgggct
     7321 atctggacaa gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag tgggcttaca
     7381 tggcgatagc tagactgggc ggttttatgg acagcaagcg aaccggaatt gccagctggg
     7441 gcgccctctg gtaaggttgg gaagccctgc aaagtaaact ggatggcttt cttgccgcca
     7501 aggatctgat ggcgcagggg atcaagctct gatcaagaga caggatgagg atcgtttcgc
     7561 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     7621 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     7681 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     7741 caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     7801 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     7861 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     7921 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     7981 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     8041 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac
     8101 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     8161 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     8221 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     8281 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     8341 gacgagttct tctgaatttt gttaaaattt ttgttaaatc agctcatttt ttaaccaata
     8401 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
     8461 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
     8521 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
     8581 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
     8641 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
     8701 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
     8761 taatgcgccg ctacagggcg cgtccattcg ccattcagga tcgaattaat tcttaattaa
     8821 catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt
     8881 ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt
     8941 gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg
     9001 gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag
     9061 taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga
     9121 agtgaaatct gaataatttt gtgttactca tagcgcgtaa tactgtaata gtaatcaatt
     9181 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     9241 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     9301 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     9361 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     9421 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     9481 acttggcagt acatctacgt attagtcatc gctattacca tg
//
