LOCUS       sequence-21565-e         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  TITLE     T4 Lysozyme Plasmids - Tray 1
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: T4 Lysozyme I3C I9C T21C C54T T142C L164C
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(711..733)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    722..738
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 actnaaaaag cccgctcatt aggcgggctc agatctgctc atgtttgaca gcttatcatc
       61 gataagcttc ggcaataaat tcttgatatg ttttcatatg tttcctttaa atattttaat
      121 tagtaattgt ctattcaagt aattgtgaat atactatcac aattccaaga gaaagtaaac
      181 agctttaaca atttttatac gcgtcccaag tgccagttct aaacgttgta atgactcgtt
      241 ttgcgcgatt aggacattga ttataccatc tacttttagc taagttaact gctgcttcat
      301 cccagcgttt ttgttgaagc atacgtaaag agttagtaaa tcctgccaca ccggtttctc
      361 ccatttggaa aaccatatta atcaatgcac agcgacgaac cgcatcaaga gaatcataaa
      421 ccggttttaa tttagcattt ctcagaattc cgcgaacagc agcatcaaca tcctgattaa
      481 agagtttttc agcctcatct tttgtaatta caccattagt attacgccca atagctttat
      541 ctaattcaga tttagcagca ttaagtgatg gactttttgt aagcaaatga ccgatgccaa
      601 tagtgtaata gccttcacag tctttataga ttttaagtct aagaccttca tcacaacgta
      661 acatttcaaa acaattcata atacctccta agcatcgatg gatcctgttt cctgtgtgaa
      721 attgttatcc gctcacaatt ccacacatta tacgagccga tgataatgtc acaggggagg
      781 gagagcccgc agnggaaccc gnacagggca agctgttcng tggaatgtan cgncnatcnc
      841 cataacagcg gagna
//
