LOCUS       sequence-215668-        1751 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BTK Type 2 promoter for golden gate assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Leonard SP, Perutka J, Powell JE, Geng P, Richhart DD, Byrom M, Kar 
            S, Davies BW, Ellington AD, Moran NA, Barrick JE
  TITLE     Genetic Engineering of Bee Gut Microbiome Bacteria with a Toolkit 
            for Modular Assembly of Broad-Host-Range Plasmids.
  JOURNAL   ACS Synth Biol. 2018 May 18;7(5):1279-1290. doi: 
            10.1021/acssynbio.7b00399. Epub 2018 Apr 13.
  PUBMED    29608282
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.7b00399"; journalName: "ACS Synth Biol"; date: 
            "2018-05-18- 18"; volume: "7"; issue: "5"; pages: "1279-1290"
COMMENT     Sequence Label: pBTK113
FEATURES             Location/Qualifiers
     source          1..1751
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      88..676
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     577..596
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(893..927)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(998..1657)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1572..1591
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tgcccgatca atcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
       61 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
      121 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
      181 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
      241 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
      301 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
      361 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
      421 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
      481 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
      541 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
      601 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
      661 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
      721 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtagtcg
      781 gtctcaaacg catacgggag tttattcttg acatattgcc ggtgtgttgg tataataact
      841 tagtactgtt atacagaaac agaggagata ttacatatgt gagaccagac caataaaaaa
      901 cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg
      961 atctatcaac aggagtccaa gcgagctcga tatcaaatta cgccccgccc tgccactcat
     1021 cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca caaacggcat
     1081 gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca
     1141 tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga
     1201 aactcaccca gggattggct gaaacgaaaa acatattctc aataaaccct ttagggaaat
     1261 aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga
     1321 aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca tggaaaacgg
     1381 tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgaa
     1441 attccggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt
     1501 gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat
     1561 aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata
     1621 tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa
     1681 atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg
     1741 aacctcttac g
//
