LOCUS       sequence-215672-        9260 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cre-dependent activation of gene of interest by removing Stop 
            sequences; Puro selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9260)
  AUTHORS   Jackson EL, Willis N, Mercer K, Bronson RT, Crowley D, Montoya R, 
            Jacks T, Tuveson DA
  TITLE     Analysis of lung tumor initiation and progression using conditional 
            expression of oncogenic K-ras.
  JOURNAL   Genes Dev. 2001 Dec 15. 15(24):3243-8.
  PUBMED    11751630
REFERENCE   2  (bases 1 to 9260)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2001 Dec 15. 15(24):3243-8."
COMMENT     Sequence Label: Lox-Stop-Lox TOPO
FEATURES             Location/Qualifiers
     source          1..9260
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        239..257
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     239..256
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    359..392
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      423..432
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    457..578
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(1003..1023)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          1153..1218
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    1310..1431
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(1856..1876)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          2006..2071
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    2163..2284
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(2709..2729)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          2859..2924
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    3016..3137
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(3562..3582)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          3712..3777
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     promoter        4387..4886
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4422..4445)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4819..4838
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4930..5529
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4930..4949)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5426..5446
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    6070..6103
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(6148..6167)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6149..6167)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6174..6191)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6174..6190)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6183..6205)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             6328..6630
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     6509..6528
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             6979..7773
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6979..7773
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7033..7052)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7643..7662
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     regulatory      7973..7982
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7979..8353
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     rep_origin      8491..9079
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8980..8999
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9233..9250
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat
      241 ttaggtgaca ctatagaata ctcaagctat gcatcaagct tggtaccaac tcgagttaat
      301 taaccacgcg tggcgcgcct tgagctcgga tccgggcccc ccctcgatcg aggtcgacat
      361 aacttcgtat aatgtatgct atacgaagtt attaggtccc tcgacctgca gacttactca
      421 agccatggtg gctagcttgc ggaattccag acatgataag atacattgat gagtttggac
      481 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
      541 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
      601 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
      661 aatgtggtat ggctgattat gatctctagt caaggcacta tacatcaaat attccttatt
      721 aaccccttta caaattaaaa agctaaaggt acacaatttt tgagcatagt tattaatagc
      781 agacactcta tgcctgtgtg gagtaagaaa aaacagtatg ttatgattat aactgttatg
      841 cctacttata aaggttacag aatatttttc cataattttc ttgtatagca gtgcagcttt
      901 ttcctttgtg gtgtaaatag caaagcaagc aagagttcta ttactaaaca cagcatgact
      961 caaaaaactt agcaattctg aaggaaagtc cttggggtct tctacctttc tcttcttttt
     1021 tggaggagta gaatgttgag agtcagcagt agcctcatca tcactagatg gcatttcttc
     1081 tgagcaaaac aggttttcct cattaaaggc attccaccac tgctcccatt catcagttcc
     1141 ataggttgga atctaaaata cacaaacaat tagaatcagt agtttaacac attatacact
     1201 taaaaatttt atatttacct tagagcttta aatctctgta ggtagtttgt ccaattatgt
     1261 cacaccacag aagtaaggtt ccttcacaaa gatccctcga ccagacatat aagatacatt
     1321 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
     1381 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
     1441 aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag
     1501 taaaacctct acaaatgtgg tatggctgat tatgatctct agtcaaggca ctatacatca
     1561 aatattcctt attaacccct ttacaaatta aaaagctaaa ggtacacaat ttttgagcat
     1621 agttattaat agcagacact ctatgcctgt gtggagtaag aaaaaacagt atgttatgat
     1681 tataactgtt atgcctactt ataaaggtta cagaatattt ttccataatt ttcttgtata
     1741 gcagtgcagc tttttccttt gtggtgtaaa tagcaaagca agcaagagtt ctattactaa
     1801 acacagcatg actcaaaaaa cttagcaatt ctgaaggaaa gtccttgggg tcttctacct
     1861 ttctcttctt ttttggagga gtagaatgtt gagagtcagc agtagcctca tcatcactag
     1921 atggcatttc ttctgagcaa aacaggtttt cctcattaaa ggcattccac cactgctccc
     1981 attcatcagt tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa
     2041 cacattatac acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt
     2101 tgtccaatta tgtcacacca cagaagtaag gttccttcac aaagatccct cgaccagaca
     2161 tataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
     2221 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
     2281 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
     2341 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc tctagtcaag
     2401 gcactataca tcaaatattc cttattaacc cctttacaaa ttaaaaagct aaaggtacac
     2461 aatttttgag catagttatt aatagcagac actctatgcc tgtgtggagt aagaaaaaac
     2521 agtatgttat gattataact gttatgccta cttataaagg ttacagaata tttttccata
     2581 attttcttgt atagcagtgc agctttttcc tttgtggtgt aaatagcaaa gcaagcaaga
     2641 gttctattac taaacacagc atgactcaaa aaacttagca attctgaagg aaagtccttg
     2701 gggtcttcta cctttctctt cttttttgga ggagtagaat gttgagagtc agcagtagcc
     2761 tcatcatcac tagatggcat ttcttctgag caaaacaggt tttcctcatt aaaggcattc
     2821 caccactgct cccattcatc agttccatag gttggaatct aaaatacaca aacaattaga
     2881 atcagtagtt taacacatta tacacttaaa aattttatat ttaccttaga gctttaaatc
     2941 tctgtaggta gtttgtccaa ttatgtcaca ccacagaagt aaggttcctt cacaaagatc
     3001 cctcgaccag acatataaga tacattgatg agtttggaca aaccacaact agaatgcagt
     3061 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
     3121 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
     3181 aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg
     3241 atctctagtc aaggcactat acatcaaata ttccttatta acccctttac aaattaaaaa
     3301 gctaaaggta cacaattttt gagcatagtt attaatagca gacactctat gcctgtgtgg
     3361 agtaagaaaa aacagtatgt tatgattata actgttatgc ctacttataa aggttacaga
     3421 atatttttcc ataattttct tgtatagcag tgcagctttt tcctttgtgg tgtaaatagc
     3481 aaagcaagca agagttctat tactaaacac agcatgactc aaaaaactta gcaattctga
     3541 aggaaagtcc ttggggtctt ctacctttct cttctttttt ggaggagtag aatgttgaga
     3601 gtcagcagta gcctcatcat cactagatgg catttcttct gagcaaaaca ggttttcctc
     3661 attaaaggca ttccaccact gctcccattc atcagttcca taggttggaa tctaaaatac
     3721 acaaacaatt agaatcagta gtttaacaca ttatacactt aaaaatttta tatttacctt
     3781 agagctttaa atctctgtag gtagtttgtc caattatgtc acaccacaga agtaaggttc
     3841 cttcacaaag atccctcgat cgagaaaaaa aatataaaag agatggagga acgggaaaaa
     3901 gttagttgtg gtgataggtg gcaagtggta ttccgtaaga acaacaagaa aagcatttca
     3961 tattatggct gaactgagcg aacaagtgca aaatttaagc atcaacgaca acaacgagaa
     4021 tggttatgtt cctcctcact taagaggaaa accaagaagt gccagaaata acagtagcaa
     4081 ctacaataac aacaacggcg gctacaacgg tggccgtggc ggtggcagct tctttagcaa
     4141 caaccgtcgt ggtggttacg gcaacggtgg tttcttcggt ggaaacaacg gtggcagcag
     4201 atctaacggc cgttctggtg gtagatggat cgatggcaaa catgtcccag ctccaagaaa
     4261 cgaaaaggcc gagatcgcca tatttggtgt ccccgaggat ccctgtggaa aaaaaaggga
     4321 caggataagt atgacatcat caaggaaacc ctggactact gcgcccttcg acctcgaaat
     4381 tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc
     4441 cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg
     4501 gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc
     4561 tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag
     4621 tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag
     4681 gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg
     4741 gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa
     4801 ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct
     4861 cctcttcctc atctccgggc ctttcgacct gcatccatct agatctcgat cgagcagctg
     4921 aagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc
     4981 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc
     5041 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc
     5101 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc
     5161 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag
     5221 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg
     5281 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag
     5341 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc
     5401 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc
     5461 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc
     5521 ggtgcctgac gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc acgaccccat
     5581 gcatcgatga tatcagatcc ccgggatgca gaaattgatg atctattaaa caataaagat
     5641 gtccactaaa atggaagttt ttcctgtcat actttgttaa gaagggtgag aacagagtac
     5701 ctacattttg aatggaagga ttggagctac gggggtgggg gtggggtggg attagataaa
     5761 tgcctgctct ttactgaagg ctctttacta ttgctttatg ataatgtttc atagttggat
     5821 atcataattt aaacaagcaa aaccaaatta agggccagct cattcctccc actcatgatc
     5881 tatagatcta tagatctctc gtgggatcat tgtttttctc ttgattccca ctttgtggtt
     5941 ctaagtactg tggtttccaa atgtgtcagt ttcatagcct gaagaacgag atcagcagcc
     6001 tctgttccac atacacttca ttctcagtat tgttttgcca agttctaatt ccatcagaag
     6061 ctggtcgaga taacttcgta taatgtatgc tatacgaagt tatgtcgaca gcggccgctc
     6121 gagcatgcat ctagagggcc caattcgccc tatagtgagt cgtattacaa ttcactggcc
     6181 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     6241 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     6301 caacagttgc gcagcctata cgtacggcag tttaaggttt acacctataa aagagagagc
     6361 cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgccggg gcgacggatg
     6421 gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg
     6481 gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg
     6541 gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac
     6601 gccattaacc tgatgttctg gggaatataa atgtcaggca tgagattatc aaaaaggatc
     6661 ttcacctaga tccttttcac gtagaaagcc agtccgcaga aacggtgctg accccggatg
     6721 aatgtcagct actgggctat ctggacaagg gaaaacgcaa gcgcaaagag aaagcaggta
     6781 gcttgcagtg ggcttacatg gcgatagcta gactgggcgg ttttatggac agcaagcgaa
     6841 ccggaattgc cagctggggc gccctctggt aaggttggga agccctgcaa agtaaactgg
     6901 atggctttct tgccgccaag gatctgatgg cgcaggggat caagctctga tcaagagaca
     6961 ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct
     7021 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
     7081 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
     7141 ggtgccctga atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc
     7201 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
     7261 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
     7321 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
     7381 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
     7441 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
     7501 aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
     7561 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
     7621 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
     7681 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
     7741 gccttctatc gccttcttga cgagttcttc tgaattatta acgcttacaa tttcctgatg
     7801 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatcaggtg gcacttttcg
     7861 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     7921 gctcatgaga caataaccct gataaatgct tcaataatag cacgtgagga gggccaccat
     7981 ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga
     8041 gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt
     8101 ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa
     8161 caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt
     8221 cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc
     8281 gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga
     8341 ggagcaggac tgacacgtgc taaaacttca tttttaattt aaaaggatct aggtgaagat
     8401 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     8461 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     8521 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     8581 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     8641 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     8701 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     8761 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     8821 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     8881 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     8941 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     9001 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     9061 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     9121 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     9181 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     9241 agtgagcgag gaagcggaag
//
