LOCUS       sequence-215673-        4184 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid contains a human pancreatic islet active enhancer, 
            which can be shuttled into a Gateway destination vector, such as 
            pGL4.23-Gateway..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4184)
  AUTHORS   Pasquali L, Gaulton KJ, Rodriguez-Segui SA, Mularoni L, 
            Miguel-Escalada I, Akerman I, Tena JJ, Moran I, Gomez-Marin C, van 
            de Bunt M, Ponsa-Cobas J, Castro N, Nammo T, Cebola I, 
            Garcia-Hurtado J, Maestro MA, Pattou F, Piemonti L, Berney T, Gloyn 
            AL, Ravassard P, Gomez-Skarmeta JL, Muller F, McCarthy MI, Ferrer J
  TITLE     Pancreatic islet enhancer clusters enriched in type 2 diabetes 
            risk-associated variants.
  JOURNAL   Nat Genet. 2014 Feb;46(2):136-43. doi: 10.1038/ng.2870. Epub 2014 
            Jan 12.
  PUBMED    24413736
REFERENCE   2  (bases 1 to 4184)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ng.2870"; 
            journalName: "Nat Genet"; date: "2014-02"; volume: "46"; issue: "2";
            pages: "136-43"
COMMENT     Sequence Label: pENTR D-TOPO-C3_33
FEATURES             Location/Qualifiers
     source          1..4184
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2309..2408)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2425..2444)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2426..2444)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2449..2465)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2449..2465)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2567..2586)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2578..3387
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2645..2664)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3534..4122
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4023..4042
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc gcggccgccc ccttcaccga caggattcca tcggaagagc acctttctag
      721 ttaaaaatcc aatcacatta ttctagtcag cattttctcc tctgttctcc atcgaactac
      781 tctaccccag gctaccagta acaaaaacaa aagaaacaag aaaaagaaaa agcaaggaac
      841 atccagaggt ataaattagg atattttgga tctgacattg tggcatagct agagttttat
      901 actacattgg ttcctacaat gaaaatgttc attctgctaa tatgagtcag catgaaagtt
      961 aataaatctt tccttttgac cactttcaaa ctctttgaac acacttctgt aaatcaggtc
     1021 agagaggaaa ttacttaacc ctaaggaaac taaggcacaa aaccatttag taattccact
     1081 agtttttctg aaaatgcgtg actttaatca aaatataaaa tcatgactct accttctacc
     1141 caaggctcca ggcactgctc atcatggatt aaaagattaa acaaacgaaa agcaatgtta
     1201 actcatccat tccagaaaat ttcaagaaca tgacttccat aatgcctatt tacagctaaa
     1261 ggccctttct tagtaaatat cattaacaga cccatctaga gtcataactc atctgatgag
     1321 ccagttaatt attaattttc atacatcatt tcatttcctt aggctacatt tagcctttgt
     1381 tacattttcc atttcttatg ccacaagcac ttcttactct gcatagaaaa cccgataatg
     1441 aggcacaaaa tactggccag caaacaaact gcttctgact ctacttctta tatatctgtc
     1501 taagccatct acaataagag tttttattga tcctttttat tttttccctt tgggttcatg
     1561 actgtataat ttcattaatg ccgccttttc aacatcgctg ccttgttaat aggtccctgc
     1621 tattcccatg ggcttctctt gttacagctt ttgtagccag aagaatctag aaatgaaaag
     1681 cctattgata gataatataa gttattctca tgcacggctg cagtgtgaag cacatttcgc
     1741 aattcaatca aggacagata gagctgaatg ttcttagatc ctaggagttc atttaaataa
     1801 gcagaatgtc ccttgtccta acacaggtga ctgactcctc agaaaagact gtataaacaa
     1861 aaaccaatca cgtaagtcat tttattggca acaatataaa cacagctata ttagatgtca
     1921 ccaataaatc aaaattattc ttgtctgcaa ttttaaccag aggaaagtgc aaacatgtgc
     1981 ctgtgaaact ggaggatatt atagaggaaa tatattggat gtgtacaaca ttatttcccc
     2041 aaattgaaac tggaggatac tatattatga tatgataacc cataacaagg tagagtttga
     2101 tcatttctgt ggttccataa ttttatgcag tacaactgac aaattcatag aatttgcaac
     2161 cgaagttaca cagaaacaga actgagataa ttcctcatta ttgaaatgtg gagcagaaag
     2221 ttagggaggt tacacctaat aactagaatg tatagaccta ttcacttggg cactcccaag
     2281 gcctaacgtt ggaagggtgg gcgcgccgac ccagctttct tgtacaaagt tggcattata
     2341 agaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc
     2401 attatttgcc atccagctga tatcccctat agtgagtcgt attacatggt catagctgtt
     2461 tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat
     2521 atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg
     2581 agccatattc aacgggaaac gtcgaggccg cgattaaatt ccaacatgga tgctgattta
     2641 tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg
     2701 tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat
     2761 gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc
     2821 atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccgga
     2881 aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg
     2941 ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc
     3001 gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg
     3061 agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat
     3121 aaacttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac
     3181 cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca
     3241 gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta
     3301 cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt
     3361 catttgatgc tcgatgagtt tttctaatca gaattggtta attggttgta acactggcag
     3421 agcattacgc tgacttgacg ggacggcgca agctcatgac caaaatccct taacgtgagt
     3481 tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     3541 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     3601 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     3661 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     3721 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     3781 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     3841 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     3901 gaactgagat acctacagcg tgagcattga gaaagcgcca cgcttcccga agggagaaag
     3961 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     4021 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     4081 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     4141 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgtt
//
