LOCUS       sequence-21568-e         615 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Taganov KD, Boldin MP, Chang KJ, Baltimore D
  TITLE     NF-kappaB-dependent induction of microRNA miR-146, an inhibitor 
            targeted to signaling proteins of innate immune responses.
  JOURNAL   Proc Natl Acad Sci U S A. 2006 Aug 15. 103(33):12481-6.
  PUBMED    16885212
REFERENCE   2  (bases 1 to 615)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2006 Aug 15. 103(33):12481-6."
COMMENT     Sequence Label: miR-146a
FEATURES             Location/Qualifiers
     source          1..615
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     23..42
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        23..41
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(431..449)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(432..449)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(469..486)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    475..586
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
       61 gagctcggat cctaactcat gagtgccagg actagacctg gtactaggaa gcagctgcat
      121 tggatttacc aggcttttca ctcttgtatt ttacagggct gggacaggcc tggactgcaa
      181 ggaggggtct ttgcaccatc tctgaaaagc cgatgtgtat cctcagcttt gagaactgaa
      241 ttccatgggt tgtgtcagtg tcagacctgt gaaattcagt tcttcagctg ggatatctct
      301 gtcatcgtgg gcttgaggac ctggagagag tagatcctga agaacttttt cagtctgctg
      361 aagagcttgg aagactggag acagaaggca gagtctcagg ctcctcgagc atgcatctag
      421 agggccctat tctatagtgt cacctaaatg ctagagctcg ctgatcagcc tcgactgtgc
      481 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
      541 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
      601 ggtgtcattc tattc
//
