LOCUS       sequence-215686-        8772 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pHAGE NFkB-TA-LUC-UBC-GFP-W .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8772)
  AUTHORS   Wilson AA, Kwok LW, Porter EL, Payne JG, McElroy GS, Ohle SJ, 
            Greenhill SR, Blahna MT, Yamamoto K, Jean JC, Mizgerd JP, Kotton DN
  TITLE     Lentiviral delivery of RNAi for in vivo lineage-specific modulation 
            of gene expression in mouse lung macrophages.
  JOURNAL   Mol Ther. 2013 Apr;21(4):825-33. doi: 10.1038/mt.2013.19. Epub 2013 
            Feb 12.
  PUBMED    23403494
REFERENCE   2  (bases 1 to 8772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/mt.2013.19"; journalName: "Mol Ther"; date: "2013-04"; 
            volume: "21"; issue: "4"; pages: "825-33"
COMMENT     Sequence Label: pHAGE NFkB-TA-LUC-UBC-GFP-W
FEATURES             Location/Qualifiers
     source          1..8772
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     52..71
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             263..896
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    943..1068
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1565..1798
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1983..2027
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2320..2437
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2532..2563
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      2619..2628
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2625..4277
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(2691..2709)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     promoter        4284..4682
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     CDS             join(4691..4693,4694..4696,4697..5410)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4691..4693,4694..4696,4697..5407)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4736..4757)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4997..5016)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5344..5365
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5424..6012
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5477..5497)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5895..5906)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6087..6320
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6439..6457)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6525..6629
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6630..6698,6699..7490)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6848..6867)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7661..8249
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8150..8169
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8403..8420
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8660..8681
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8696..8701,8702..8719,8720..8726)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8734..8750
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8739..8761
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     join(8758..8772,1..2)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(8758..8772,1..2)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 acatgattac gaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
       61 ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa
      121 atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg tggtttcatt
      181 tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa
      241 tatgtactac aaacttagta gttggaaggg ctaattcact cccaaagaag acaagatatc
      301 cttgatctgt ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca
      361 gggccagggg tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag
      421 ccagataagg tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc
      481 ctgcatggga tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta
      541 gcatttcatc acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga
      601 gcttgctaca agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact
      661 ggggagtggc gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc
      721 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
      781 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
      841 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
      901 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
      961 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
     1021 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
     1081 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
     1141 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
     1201 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
     1261 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
     1321 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
     1381 aaaagtaaga ccaccgcaca gcaagcggcc ggccgctgat cttcagacct ggaggaggag
     1441 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
     1501 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
     1561 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
     1621 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
     1681 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     1741 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     1801 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
     1861 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
     1921 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
     1981 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
     2041 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
     2101 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
     2161 cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag
     2221 aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac
     2281 ggtatcgccg aattcacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2341 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2401 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2461 agatccagtt tggactagtg ggaatttccg ggaatttccg ggaatttccg ggaatttcca
     2521 gatctagact ctagagggta tataatggaa gctcgaattc cagcttggca ttccggtact
     2581 gttggtaaaa agcttggcat tccggtactg ttggtaaagc caccatggaa gacgccaaaa
     2641 acataaagaa aggcccggcg ccattctatc cgctggaaga tggaaccgct ggagagcaac
     2701 tgcataaggc tatgaagaga tacgccctgg ttcctggaac aattgctttt acagatgcac
     2761 atatcgaggt ggacatcact tacgctgagt acttcgaaat gtccgttcgg ttggcagaag
     2821 ctatgaaacg atatgggctg aatacaaatc acagaatcgt cgtatgcagt gaaaactctc
     2881 ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg agttgcagtt gcgcccgcga
     2941 acgacattta taatgaacgt gaattgctca acagtatggg catttcgcag cctaccgtgg
     3001 tgttcgtttc caaaaagggg ttgcaaaaaa ttttgaacgt gcaaaaaaag ctcccaatca
     3061 tccaaaaaat tattatcatg gattctaaaa cggattacca gggatttcag tcgatgtaca
     3121 cgttcgtcac atctcatcta cctcccggtt ttaatgaata cgattttgtg ccagagtcct
     3181 tcgataggga caagacaatt gcactgatca tgaactcctc tggatctact ggtctgccta
     3241 aaggtgtcgc tctgcctcat agaactgcct gcgtgagatt ctcgcatgcc agagatccta
     3301 tttttggcaa tcaaatcatt ccggatactg cgattttaag tgttgttcca ttccatcacg
     3361 gttttggaat gtttactaca ctcggatatt tgatatgtgg atttcgagtc gtcttaatgt
     3421 atagatttga agaagagctg tttctgagga gccttcagga ttacaagatt caaagtgcgc
     3481 tgctggtgcc aaccctattc tccttcttcg ccaaaagcac tctgattgac aaatacgatt
     3541 tatctaattt acacgaaatt gcttctggtg gcgctcccct ctctaaggaa gtcggggaag
     3601 cggttgccaa gaggttccat ctgccaggta tcaggcaagg atatgggctc actgagacta
     3661 catcagctat tctgattaca cccgaggggg atgataaacc gggcgcggtc ggtaaagttg
     3721 ttccattttt tgaagcgaag gttgtggatc tggataccgg gaaaacgctg ggcgttaatc
     3781 aaagaggcga actgtgtgtg agaggtccta tgattatgtc cggttatgta aacaatccgg
     3841 aagcgaccaa cgccttgatt gacaaggatg gatggctaca ttctggagac atagcttact
     3901 gggacgaaga cgaacacttc ttcatcgttg accgcctgaa gtctctgatt aagtacaaag
     3961 gctatcaggt ggctcccgct gaattggaat ccatcttgct ccaacacccc aacatcttcg
     4021 acgcaggtgt cgcaggtctt cccgacgatg acgccggtga acttcccgcc gccgttgttg
     4081 ttttggagca cggaaagacg atgacggaaa aagagatcgt ggattacgtc gccagtcaag
     4141 taacaaccgc gaaaaagttg cgcggaggag ttgtgtttgt ggacgaagta ccgaaaggtc
     4201 ttaccggaaa actcgacgca agaaaaatca gagagatcct cataaaggcc aagaagggcg
     4261 gaaagatcgc cgtgtaagga tccggcctcc gcgccgggtt ttggcgcctc ccgcgggcgc
     4321 ccccctcctc acggcgagcg ctgccacgtc agacgaaggg cgcagcgagc gtcctgatcc
     4381 ttccgcccgg acgctcagga cagcggcccg ctgctcataa gactcggcct tagaacccca
     4441 gtatcagcag aaggacattt taggacggga cttgggtgac tctagggcac tggttttctt
     4501 tccagagagc ggaacaggcg aggaaaagta gtcccttctc ggcgattctg cggagggatc
     4561 tccgtggggc ggtgaacgcc gatgattata taaggacgcg ccgggtgtgg cacagctagt
     4621 tccgtcgcag ccgggatttg ggtcgcggtt cttgtttgtg gatcgctgtg atcgtcactt
     4681 ggccgcccat atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     4741 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     4801 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     4861 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga
     4921 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     4981 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     5041 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     5101 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa
     5161 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt
     5221 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     5281 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga
     5341 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     5401 gtacaagtaa atcgatagat cctaatcaac ctctggatta caaaatttgt gaaagattga
     5461 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     5521 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     5581 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     5641 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     5701 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     5761 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     5821 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     5881 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     5941 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     6001 ccgcctcccc gcctgagatc ctttaagacc aatgacttac aaggcagctg tagatcttag
     6061 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
     6121 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     6181 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     6241 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     6301 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     6361 tgcaaagaaa tgaatatcag agagtgagag gcccgggtta attaaggaaa gggctagatc
     6421 attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     6481 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     6541 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     6601 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     6661 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     6721 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     6781 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     6841 aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc
     6901 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     6961 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     7021 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     7081 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     7141 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     7201 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     7261 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     7321 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     7381 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     7441 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     7501 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     7561 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     7621 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     7681 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     7741 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     7801 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     7861 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     7921 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     7981 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     8041 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     8101 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     8161 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     8221 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     8281 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     8341 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     8401 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     8461 cgcgttggcc gattcattaa tgcagcaagc tcatggctga ctaatttttt ttatttatgc
     8521 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     8581 aggcctaggc ttttgcaaaa agctccccgt ggcacgacag gtttcccgac tggaaagcgg
     8641 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     8701 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     8761 gaaacagcta tg
//
