LOCUS       sequence-215690-        5074 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ProD driven mCherry expression with synTT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5074)
  AUTHORS   Cooper KG, Chong A, Starr T, Finn CE, Steele-Mortimer O
  TITLE     Predictable, Tunable Protein Production in Salmonella for Studying 
            Host-Pathogen Interactions.
  JOURNAL   Front Cell Infect Microbiol. 2017 Nov 16;7:475. doi: 
            10.3389/fcimb.2017.00475. eCollection 2017.
  PUBMED    29201859
REFERENCE   2  (bases 1 to 5074)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Cell 
            Infect Microbiol."; date: "2017-11-16"; volume: "7:475. doi"; pages:
            " 10.3389/fcimb.2017.00475. eCollection 2017"
COMMENT     Sequence Label: pCON1-ProD.mCherry
FEATURES             Location/Qualifiers
     source          1..5074
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             114..824
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(259..277)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     527..546
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      845..916
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      932..959
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     oriT            1573..1660
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     primer_bind     complement(2973..2990)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3144..3732)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3224..3243)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3903..4694,4695..4763))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4526..4545
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4764..4868)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4936..4954
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gtataatata ttcagggaga ccacaacggt ttccctctac aaataatttt gtttaacttt
       61 tactagagtc acacaggaaa gtactagtct agatttaaga aggagatata catatggtga
      121 gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca
      181 tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct
      241 acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct
      301 gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg
      361 acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga
      421 acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt
      481 tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga
      541 agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga
      601 agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca
      661 agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca
      721 agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg
      781 agggccgcca ctccaccggc ggcatggacg agctgtacaa gtaagcatgc aagcttccag
      841 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
      901 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
      961 ttataaagct ttaatgcggt agtttatcac agttaaattg ctaacgcagt caggcaccgt
     1021 gtatgaaatc taacaatgcg ctcatcgtca tcctcggcac cgtcaccctg gatgctgtag
     1081 gcataggctt ggttatgccg gtactgccgg gcctcttgcg ggatatcgtc cattccgaca
     1141 gcatcgccag tcactatggc gtgctgctag cgcttttagc cgctttagcg gcctttcccc
     1201 ctacccgaag ggtgggggcg cgtgtgcagc cccgcagggc ctgtctcggt cgatcattca
     1261 gcccggctca tccttctggc gtggcggcag accgaacaag gcgcggtcgt ggtcgcgttc
     1321 aaggtacgca tccattgccg ccatgagccg atcctccggc cactcgctgc tgttcacctt
     1381 ggccaaaatc atggccccca ccagcacctt gcgccttgtt tcgttcttgc gctcttgctg
     1441 ctgttccctt gcccgcaccc gctgaatttc ggcattgatt cgcgctcgtt gttcttcgag
     1501 cttggccagc cgatccgccg ccttgttgct ccccttaacc atcttgacac cccattgtta
     1561 atgtgctgtc tcgtaggcta tcatggaggc acagcggcgg caatcccgac cctactttgt
     1621 aggggagggc gcacttaccg gtttctcttc gagaaactgg cctaacggcc acccttcggg
     1681 cggtgcgctc tccgagggcc attgcatgga gccgaaaagc aaaagcaaca gcgaggcagc
     1741 atggcgattt atcaccttac ggcgaaaacc ggcagcaggt cgggcggcca atcggccagg
     1801 gccaaggccg actacatcca gcgcgaaggc aagtatgccc gcgacatgga tgaagtcttg
     1861 cacgccgaat ccgggcacat gccggagttc gtcgagcggc ccgccgacta ctgggatgct
     1921 gccgacctgt atgaacgcgc caatgggcgg ctgttcaagg aggtcgaatt tgccctgccg
     1981 gtcgagctga ccctcgacca gcagaaggcg ctggcgtccg agttcgccca gcacctgacc
     2041 ggtgccgagc gcctgccgta tacgctggcc atccatgccg gtggcggcga gaacccgcac
     2101 tgccacctga tgatctccga gcggatcaat gacggcatcg agcggcccgc cgctcagtgg
     2161 ttcaagcggt acaacggcaa gaccccggag aagggcgggg cacagaagac cgaagcgctc
     2221 aagcccaagg catggcttga gcagacccgc gaggcatggg ccgaccatgc caaccgggca
     2281 ttagagcggg ctggccacga cgcccgcatt gaccacagaa cacttgaggc gcagggcatc
     2341 gagcgcctgc ccggtgttca cctggggccg aacgtggtgg agatggaagg ccggggcatc
     2401 cgcaccgacc gggcagacgt ggccctgaac atcgacaccg ccaacgccca gatcatcgac
     2461 ttacaggaat accgggaggc aatagaccat gaacgcaatc gacagagtga agaaatccag
     2521 aggcatcaac gagttagcgg agcagatcga accgctggcc cagagcatgg cgacactggc
     2581 cgacgaagcc cggcaggtca tgagccagac ccagcaggcc agcgaggcgc aggcggcgga
     2641 gtggctgaaa gcccagcgcc agacaggggc ggcatgggtg gagctggcca aagagttgcg
     2701 ggaggtagcc gccgaggtga gcagcgccgc gcagagcgcc cggagcgcgt cgcgggggtg
     2761 gcactggaag ctatggctaa ccgtgatgct ggcttccatg atgcctacgg tggtgctgct
     2821 gatcgcatcg ttgctcttgc tcgacctgac gccactgaca accgaggacg gctcgatctg
     2881 gctgcgcttg gtggcccgat gaagaacgac aggactttgc aggccatagg ccgacagctc
     2941 aaggccatgg gctgtgagcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     3001 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     3061 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     3121 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     3181 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     3241 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     3301 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     3361 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     3421 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     3481 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     3541 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     3601 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     3661 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     3721 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     3781 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     3841 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     3901 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     3961 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     4021 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     4081 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     4141 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     4201 aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     4261 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     4321 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     4381 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     4441 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     4501 tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg
     4561 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     4621 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     4681 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     4741 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     4801 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     4861 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     4921 acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga attcgagctc
     4981 ggtacccggg cacagctaac accacgtcgt ccctatctgc tgccctaggt ctatgagtgg
     5041 ttgctggata actttacggg catgcataag gctc
//
