LOCUS       sequence-215694-        7929 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PsopB driven sopB-2xHA and sigE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7929)
  AUTHORS   Knodler LA, Winfree S, Drecktrah D, Ireland R, Steele-Mortimer O
  TITLE     Ubiquitination of the bacterial inositol phosphatase, SopB, 
            regulates its biological activity at the plasma membrane.
  JOURNAL   Cell Microbiol. 2009 Nov;11(11):1652-70. doi: 
            10.1111/j.1462-5822.2009.01356.x. Epub 2009 Jul 13.
  PUBMED    19614667
REFERENCE   2  (bases 1 to 7929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1462-5822.2009.01356.x"; journalName: "Cell Microbiol"; 
            date: "2009-11"; volume: "11"; issue: "11"; pages: "1652-70"
COMMENT     Sequence Label: pSopB2xHA
FEATURES             Location/Qualifiers
     source          1..7929
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1887..1913
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1914..1940
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(2373..2393)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2373..2391)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2412..2428)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2412..2428)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2425..2447)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2436..2452
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2460..2466,2467..2484,2485..2490))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2505..2526
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2643..2660)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      3805..4027
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             4075..5025
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     CDS             complement(join(6046..6837,6838..6906))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6669..6688
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6907..7011)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7037..7492)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7174..7195)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7386..7405
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7619..7641
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7633..7650
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7634..7650
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7660..7679
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7660..7678
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     7703..7719
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     7704..7720
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ttatgaacat ttgatgtacc gatctccccc atgatcgcca ctacgtatgg acgtcaggat
       61 gcctccccgc ctgatcagaa gcgtttcctc attaaaaagg acattttttt aaagttcctg
      121 gtgcataaaa gtcacatcct tttaaagggt tgttaaccct gttgaatgtt cccactcccc
      181 tattcaggaa tattaaaaac gctatgcaaa tacagagctt ctatcactca gcttcactaa
      241 aaacccagga ggcttttaaa agcctacaaa aaaccttata caacggaatg cagattctct
      301 caggccaggg caaagcgccg gctaaagcgc ccgacgctcg cccggaaatt attgtcctgc
      361 gagaacccgg cgcgacatgg gggaattatc tacagcatca gaaggcgtct aaccactcgc
      421 tgcataacct ctataactta cagcgcgatc ttcttaccgt cgcggcaacc gttctgggta
      481 aacaagaccc ggttctaacg tcaatggcaa accaaatgga gttagccaaa gttaaagcgg
      541 accggccagc aacaaaacaa gaagaagccg cggcaaaagc attgaagaaa aatcttatcg
      601 aacttattgc agcacgcact cagcagcagg atggcttacc tgcaaaagaa gctcatcgct
      661 ttgcggcagt agcgtttaga gatgctcagg tcaagcagct taataaccag ccctggcaaa
      721 ccataaaaaa tacactcacg cataacgggc atcactatac caacacgcag ctccctgcag
      781 cagagatgaa aatcggcgca aaagatatct ttcccagtgc ttatgaggga aagggcgtat
      841 gcagttggga taccaagaat attcatcacg ccaataattt gtggatgtcc acggtgagtg
      901 tgcatgagga cggtaaagat aaaacgcttt tttgcgggat acgtcatggc gtgctttccc
      961 cctatcatga aaaagatccg cttctgcgtc acgtcggcgc tgaaaacaaa gccaaagaag
     1021 tattaactgc ggcacttttt agtaaacctg agttgcttaa caaagcctta gcgggcgagg
     1081 cagtaagcct gaaactggta tccgtcgggt tactcaccgc gtcgaatatt ttcggcaaag
     1141 agggaacgat ggtcgaggac caaatgcgcg catggcaatc gttgacccag ccgggaaaaa
     1201 tgattcattt aaaaatccgc aataaagatg gcgatctaca gacggtaaaa ataaaaccgg
     1261 acgtcgccgc atttaatgtg ggtgttaatg agctggcgct caagctcggc tttggcctta
     1321 aggcatcgga tagctataat gccgaggcgc tacatcagtt attaggcaat gatttacgcc
     1381 ctgaagccag accaggtggc tgggttggcg aatggctggc gcaatacccg gataattatg
     1441 aggtcgtcaa tacattagcg cgccagatta aggatatatg gaaaaataac caacatcata
     1501 aagatggcgg cgaaccctat aaactcgcac aacgccttgc catgttagcc catgaaattg
     1561 acgcggtacc cgcctggaat tgtaaaagcg gcaaagatcg tacagggatg atggattcag
     1621 aaatcaagcg agagatcatt tccttacatc agacccatat gttaagtgcg cctggtagtc
     1681 ttccggatag cggtggacag aaaattttcc aaaaagtatt actgaatagc ggtaacctgg
     1741 agattcagaa gcaaaatacg ggcggggcgg gaaacaaagt aatgaaaaat ttatcgccag
     1801 aggtgctcaa tctttcctat caaaaacgag ttggggatga aaatatttgg cagtcagtaa
     1861 aaggcatttc ttcattaatc acatcttatc cgtatgatgt gccggattat gcgtatccgt
     1921 atgatgtgcc ggattatgcg tgagtcttga ggtaactata tggaaagtct attaaatcgt
     1981 ttatatgacg cgttaggcct ggatgcgcca gaagatgagc cactgcttat cattgatgat
     2041 gggatacagg tttattttaa tgaatccgat catacactgg aaatgtgctg tccctttatg
     2101 ccattgcctg acgacatcct gactttgcag cattttttac gtctgaacta caccagcgcc
     2161 gtcactatcg gcgctgacgc agacaatact gctttagtgg cgctttatcg cttgccgcaa
     2221 accagtaccg aagaagaggc gctcactggt tttgaattat tcatttcaaa cgtgaagcaa
     2281 ttgaaagagc attatgcata atttaatacg tcaacatact ttcttaatga gctagagcgg
     2341 ccgccaccgc ggtggagctc cagcttttgt tccctttagt gagggttaat tgcgcgcttg
     2401 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     2461 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     2521 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     2581 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     2641 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     2701 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     2761 gatctctacg ggtcggattt gaagtcgtct tggtaggagg cagcctgaat ggcgaatgcc
     2821 gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat
     2881 cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg gtgccggcag
     2941 cgctctgggt cattttcggc gaggaccgct ttcgctggag cgcgacgatg atcggcctgt
     3001 cgcttgcggt attcggaatc ttgcacgccc tcgctcaagc cttcgtcact ggtcccgcca
     3061 ccaaacgttt cggcgagaag caggccatta tcgccggcat ggcggccgac gcgctgggct
     3121 acgtcttgct ggcgttcgcg acgcgaggct ggatggcctt ccccattatg attcttctcg
     3181 cttccggcgg catcgggatg cccgcgttgc aggccatgct gtccaggcag gtagatgacg
     3241 accatcaggg acagcttcaa ggatcgctcg cggctcttac cagcctaact tcgatcattg
     3301 gaccgctgat cgtcacggcg atttatgccg cctcggcgag cacatggaac gggttggcat
     3361 ggattgtagg cgccgcccta taccttgtct gcctccccgc gttgcgtcgc ggtgcatgga
     3421 gccgggccac ctcgacctga atggaagccg gcggcacctc gctaacggat tcaccactcc
     3481 gcagacccgc cataaaacgc cctgagaagc ccgtgacggg cttttcttgt attatgggta
     3541 gtttccttgc atgaatccat aaaaggcgcc tgtagtgcca tttaccccca ttcactgcca
     3601 gagccgtgag cgcagcgaac tgaatgtcac gaaaaagaca gcgactcagg tgcctgatgg
     3661 tcggagacaa aaggaatatt cagcgatttg cccgagcttg cgagggtgct acttaagcct
     3721 ttagggtttt aaggtctgtt ttgtagagga gcaaacagcg tttgcgacat ccttttgtaa
     3781 tactgcggaa ctgactaaag tagtgagtta tacacagggc tgggatctat tctttttatc
     3841 tttttttatt ctttctttat tctataaatt ataaccactt gaatataaac aaaaaaaaca
     3901 cacaaaggtc tagcggaatt tacagagggt ctagcagaat ttacaagttt tccagcaaag
     3961 gtctagcaga atttacagat acccacaact caaaggaaaa ggactagtaa ttatcattga
     4021 ctagcccatc tcaattggta tagtgattaa aatcacctag accaattgag atgtatgtct
     4081 gaattagttg ttttcaaagc aaatgaacta gcgattagtc gctatgactt aacggagcat
     4141 gaaaccaagc taattttatg ctgtgtggca ctactcaacc ccacgattga aaaccctaca
     4201 aggaaagaac ggacggtatc gttcacttat aaccaatacg ctcagatgat gaacatcagt
     4261 agggaaaatg cttatggtgt attagctaaa gcaaccagag agctgatgac gagaactgtg
     4321 gaaatcagga atcctttggt taaaggcttt gagattttcc agtggacaaa ctatgccaag
     4381 ttctcaagcg aaaaattaga attagttttt agtgaagaga tattgcctta tcttttccag
     4441 ttaaaaaaat tcataaaata taatctggaa catgttaagt cttttgaaaa caaatactct
     4501 atgaggattt atgagtggtt attaaaagaa ctaacacaaa agaaaactca caaggcaaat
     4561 atagagatta gccttgatga atttaagttc atgttaatgc ttgaaaataa ctaccatgag
     4621 tttaaaaggc ttaaccaatg ggttttgaaa ccaataagta aagatttaaa cacttacagc
     4681 aatatgaaat tggtggttga taagcgaggc cgcccgactg atacgttgat tttccaagtt
     4741 gaactagata gacaaatgga tctcgtaacc gaacttgaga acaaccagat aaaaatgaat
     4801 ggtgacaaaa taccaacaac cattacatca gattcctacc tacataacgg actaagaaaa
     4861 acactacacg atgctttaac tgcaaaaatt cagctcacca gttttgaggc aaaatttttg
     4921 agtgacatgc aaagtaagta tgatctcaat ggttcgttct catggctcac gcaaaaacaa
     4981 cgaaccacac tagagaacat actggctaaa tacggaagga tctgaggttc ttatggctct
     5041 tgtatctatc agtgaagcat caagactaac aaacaaaagt agaacaactg ttcaccgtta
     5101 catatcaaag ggaaaactgt ccatatgcac agatgaaaac ggtgtaaaaa agatagatac
     5161 atcagagctt ttacgagttt ttggtgcatt caaagctgtt caccatgaac agatcgacaa
     5221 tgtaacagat gaacagcatg taacacctaa tagaacaggt gaaaccagta aaacaaagca
     5281 actagaacat gaaattgaac acctgagaca acttgttaca gctcaacagt cacacataga
     5341 cagcctgaaa caggcgatgc tgcttatcga atcaaagctg ccgacaacac gggagccagt
     5401 gacgcctccc gtggggaaaa aatcatggca attctggaag aaatagcgct ttcagccggc
     5461 aaaccggctg aagccggatc tgcgattctg ataacaaact agcaacacca gaacagcccg
     5521 tttgcgggca gcaaaacccg tacttttgga cgttccggcg gttttttgtg gcgagtggtg
     5581 ttcgggcggt gcgcgcaaga tccattatgt taaacgggcg agtttacatc tcaaaaccgc
     5641 ccgcttaaca ccatcagaaa tcctcagcgc gattttaagc accaaccccc ccccgtaaca
     5701 cccaaatcca tactgaaagt ggctttgttg aataaatcag atttcgggta agtctccccc
     5761 gtagcgggtt gtgttttcag gcaatacgca cgctttcagg catacctgct ttcgtcattt
     5821 tgttcagcgc tcgtaccagg gccatagcct ccgcaacctg accatcgtag tcacgcagcg
     5881 tcagtgaacc cccgaacaga gatctgacgc tcagtggaac gaaaactcac gttaagggat
     5941 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     6001 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     6061 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     6121 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     6181 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     6241 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     6301 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     6361 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     6421 gcgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     6481 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     6541 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     6601 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     6661 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     6721 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     6781 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     6841 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     6901 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     6961 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     7021 ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
     7081 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
     7141 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
     7201 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
     7261 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
     7321 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
     7381 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
     7441 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg
     7501 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
     7561 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
     7621 cagtcacgac gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc
     7681 gaattgggta ccgggccccc cctcgaggtc gacggtatcg ataagctttc tgttcaagca
     7741 tggaatagga aaaacgaata ttcttcgtca cggtcttact tgtccggggc tttgctggca
     7801 tacacacacc tgtataacat ttgatgtaac gccgttactt tacgcaggag taaatcggtg
     7861 aatttgatct gagtcaagaa ggtgggtttt caataaaagt tgtgccataa attgtgaagt
     7921 ttgtagatt
//
