LOCUS       sequence-215698-        9393 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9393)
  AUTHORS   Pegoraro G, Kubben N, Wickert U, Gohler H, Hoffmann K, Misteli T
  TITLE     Ageing-related chromatin defects through loss of the NURD complex.
  JOURNAL   Nat Cell Biol. 2009 Oct . 11(10):1261-7.
  PUBMED    19734887
REFERENCE   2  (bases 1 to 9393)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2009 Oct . 11(10):1261-7."
COMMENT     Sequence Label: pCDHblast MCSNard OST-LMNA
FEATURES             Location/Qualifiers
     source          1..9393
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1802..1918
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2008..2211
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2208..2232
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2312..2335
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             2372..2395
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     promoter        4521..4732
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             4745..5013
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             5045..5443
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    5467..6055
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5520..5540)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5938..5949)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6129..6362
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6434..6555
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6471..6490)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6525..6544
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6595..6730
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6763..6779)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6763..6779)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6776..6798)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6787..6803
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6811..6817,6818..6835,6836..6841))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6856..6877
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6994..7011)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7165..7753)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(7924..8715,8716..8784))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8547..8566
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8785..8889)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8957..8975
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9013..9035)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9135..9154
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9348..9370
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9362..9379
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9363..9379
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccactgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     1741 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa
     1801 cttttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     1861 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaattc aaaattttat
     1921 cgatactagt attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     1981 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
     2041 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
     2101 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
     2161 acgcaaatgg gcggtaggcg tgtacggtgg gaggtttata taagcagagc tcgtttagtg
     2221 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagattctag
     2281 agctagcgaa ttgggatcca ccatggctag ctggagccac ccgcagttcg agaaaggtgg
     2341 aggttccgga ggtggatcgg gaggtggatc gtggagccac ccgcagttcg aaaaaggcgc
     2401 cgggtcgacc gagaccccgt cccagcggcg cgccacccgc agcggggcgc aggccagctc
     2461 cactccgctg tcgcccaccc gcatcacccg gctgcaggag aaggaggacc tgcaggagct
     2521 caatgatcgc ttggcggtct acatcgaccg tgtgcgctcg ctggaaacgg agaacgcagg
     2581 gctgcgcctt cgcatcaccg agtctgaaga ggtggtcagc cgcgaggtgt ccggcatcaa
     2641 ggccgcctac gaggccgagc tcggggatgc ccgcaagacc cttgactcag tagccaagga
     2701 gcgcgcccgc ctgcagctgg agctgagcaa agtgcgtgag gagtttaagg agctgaaagc
     2761 gcgcaatacc aagaaggagg gtgacctgat agctgctcag gctcggctga aggacctgga
     2821 ggctctgctg aactccaagg aggccgcact gagcactgct ctcagtgaga agcgcacgct
     2881 ggagggcgag ctgcatgatc tgcggggcca ggtggccaag cttgaggcag ccctaggtga
     2941 ggccaagaag caacttcagg atgagatgct gcggcgggtg gatgctgaga acaggctgca
     3001 gaccatgaag gaggaactgg acttccagaa gaacatctac agtgaggagc tgcgtgagac
     3061 caagcgccgt catgagaccc gactggtgga gattgacaat gggaagcagc gtgagtttga
     3121 gagccggctg gcggatgcgc tgcaggaact gcgggcccag catgaggacc aggtggagca
     3181 gtataagaag gagctggaga agacttattc tgccaagctg gacaatgcca ggcagtctgc
     3241 tgagaggaac agcaacctgg tgggggctgc ccacgaggag ctgcagcagt cgcgcatccg
     3301 catcgacagc ctctctgccc agctcagcca gctccagaag cagctggcag ccaaggaggc
     3361 gaagcttcga gacctggagg actcactggc ccgtgagcgg gacaccagcc ggcggctgct
     3421 ggcggaaaag gagcgggaga tggccgagat gcgggcaagg atgcagcagc agctggacga
     3481 gtaccaggag cttctggaca tcaagctggc cctggacatg gagatccacg cctaccgcaa
     3541 gctcttggag ggcgaggagg agaggctacg cctgtccccc agccctacct cgcagcgcag
     3601 ccgtggccgt gcttcctctc actcatccca gacacagggt gggggcagcg tcaccaaaaa
     3661 gcgcaaactg gagtccactg agagccgcag cagcttctca cagcacgcac gcactagcgg
     3721 gcgcgtggcc gtggaggagg tggatgagga gggcaagttt gtccggctgc gcaacaagtc
     3781 caatgaggac cagtccatgg gcaattggca gatcaagcgc cagaatggag atgatccctt
     3841 gctgacttac cggttcccac caaagttcac cctgaaggct gggcaggtgg tgacgatctg
     3901 ggctgcagga gctggggcca cccacagccc ccctaccgac ctggtgtgga aggcacagaa
     3961 cacctggggc tgcgggaaca gcctgcgtac ggctctcatc aactccactg gggaagaagt
     4021 ggccatgcgc aagctggtgc gctcagtgac tgtggttgag gacgacgagg atgaggatgg
     4081 agatgacctg ctccatcacc accacggctc ccactgcagc agctcggggg accccgctga
     4141 gtacaacctg cgctcgcgca ccgtgctgtg cgggacctgc gggcagcctg ccgacaaggc
     4201 atctgccagc ggctcaggag cccaggtggg cggacccatc tcctctggct cttctgcctc
     4261 cagtgtcacg gtcactcgca gctaccgcag tgtggggggc agtgggggtg gcagcttcgg
     4321 ggacaatctg gtcacccgct cctacctcct gggcaactcc agcccccgaa cccagagccc
     4381 ccagaactgc agcatcatgt aatctgtcta gaagaattcc tcgaggcggc cgctaaatag
     4441 ttgaatttaa atagatctcc agcacagtgg taaatagttg attcgaacaa ggatctgcga
     4501 tcgctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
     4561 gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag
     4621 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
     4681 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct
     4741 tcgaggggct cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg
     4801 ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt
     4861 ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct
     4921 acctagactc agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt
     4981 tgtttcgttt tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacgcccat
     5041 gggcatggcc aagcctttgt ctcaagaaga atccaccctc attgaaagag caacggctac
     5101 aatcaacagc atccccatct ctgaagacta cagcgtcgcc agcgcagctc tctctagcga
     5161 cggccgcatc ttcactggtg tcaatgtata tcattttact gggggacctt gtgcagaact
     5221 cgtggtgctg ggcactgctg ctgctgcggc agctggcaac ctgacttgta tcgtcgcgat
     5281 cggaaatgag aacaggggca tcttgagccc ctgcggacgg tgccgacagg tgcttctcga
     5341 tctgcatcct gggatcaaag ccatagtgaa ggacagtgat ggacagccga cggcagttgg
     5401 gattcgtgaa ttgctgccct ctggttatgt gtgggagggc taagcacaat tcgagctcgc
     5461 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     5521 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     5581 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     5641 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     5701 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     5761 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     5821 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     5881 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     5941 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     6001 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctggt
     6061 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     6121 ggggggactg gaagggctaa ttcactccca acgaagataa gatctgcttt ttgcttgtac
     6181 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     6241 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     6301 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     6361 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
     6421 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     6481 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     6541 catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg
     6601 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     6661 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     6721 tttggaggcc tagacttttg cagagaccaa attcgtaatc atgtcatagc tgtttcctgt
     6781 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     6841 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     6901 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     6961 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     7021 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     7081 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     7141 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     7201 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     7261 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     7321 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     7381 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     7441 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     7501 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     7561 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     7621 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     7681 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     7741 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     7801 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     7861 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     7921 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     7981 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     8041 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     8101 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     8161 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     8221 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     8281 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     8341 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     8401 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     8461 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     8521 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     8581 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     8641 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     8701 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     8761 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     8821 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     8881 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
     8941 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga
     9001 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
     9061 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
     9121 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
     9181 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct
     9241 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     9301 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     9361 ttgtaaaacg acggccagtg ccaagctgac gcg
//
