LOCUS       sequence-215699-        7874 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PsopB driven sopB (catalytically inactive) and sigE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7874)
  AUTHORS   Finn CE, Chong A, Cooper KG, Starr T, Steele-Mortimer O
  TITLE     A second wave of Salmonella T3SS1 activity prolongs the lifespan of 
            infected epithelial cells.
  JOURNAL   PLoS Pathog. 2017 Apr 20;13(4):e1006354. doi: 
            10.1371/journal.ppat.1006354. eCollection 2017 Apr.
  PUBMED    28426838
REFERENCE   2  (bases 1 to 7874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2017-04-20"; volume: "13(4):e1006354. doi"; pages: 
            " 10.1371/journal.ppat.1006354. eCollection 2017 Apr"
COMMENT     Sequence Label: pSopBC460S
FEATURES             Location/Qualifiers
     source          1..7874
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2544..2564)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2544..2562)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2583..2599)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2583..2599)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2596..2618)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2607..2623
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2631..2637,2638..2655,2656..2661))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2676..2697
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2814..2831)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      3976..4198
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             4246..5196
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     CDS             complement(join(6217..7008,7009..7077))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6840..6859
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7078..7182)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7208..7663)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7345..7366)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7557..7576
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7790..7812
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7804..7821
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7805..7821
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7831..7850
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7831..7849
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     join(7874,1..16)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 cgaggtcgac ggtatcgata agctttctgt caagcatgga ataggaaaaa cgaatattct
       61 tcgtcacggt cttacttgtc cggggctttg ctggcataca cacacctgta taacatttga
      121 tgtaacgccg ttactttacg caggagtaaa tcggtgaatt tgatctgagt caagaaggtg
      181 ggttttcaat aaaagttgtg ccataaattg tgaagtttgt agattttatg aacatttgat
      241 gtaccgatct cccccatgat cgccactacg tatggacgtc aggatgcctc cccgcctgat
      301 cagaagcgtt tcctcattaa aaaggacatt tttttaaagt tcctggtgca taaaagtcac
      361 atccttttaa agggttgtta accctgttga atgttcccac tcccctattc aggaatatta
      421 aaaacgctat gcaaatacag agcttctatc actcagcttc actaaaaacc caggaggctt
      481 ttaaaagcct acaaaaaacc ttatacaacg gaatgcagat tctctcaggc cagggcaaag
      541 cgccggctaa agcgcccgac gctcgcccgg aaattattgt cctgcgagaa cccggcgcga
      601 catgggggaa ttatctacag catcagaagg cgtctaacca ctcgctgcat aacctctata
      661 acttacagcg cgatcttctt accgtcgcgg caaccgttct gggtaaacaa gacccggttc
      721 taacgtcaat ggcaaaccaa atggagttag ccaaagttaa agcggaccgg ccagcaacaa
      781 aacaagaaga agccgcggca aaagcattga agaaaaatct tatcgaactt attgcagcac
      841 gcactcagca gcaggatggc ttacctgcaa aagaagctca tcgctttgcg gcagtagcgt
      901 ttagagatgc tcaggtcaag cagcttaata accagccctg gcaaaccata aaaaatacac
      961 tcacgcataa cgggcatcac tataccaaca cgcagctccc tgcagcagag atgaaaatcg
     1021 gcgcaaaaga tatctttccc agtgcttatg agggaaaggg cgtatgcagt tgggatacca
     1081 agaatattca tcacgccaat aatttgtgga tgtccacggt gagtgtgcat gaggacggta
     1141 aagataaaac gcttttttgc gggatacgtc atggcgtgct ttccccctat catgaaaaag
     1201 atccgcttct gcgtcacgtc ggcgctgaaa acaaagccaa agaagtatta actgcggcac
     1261 tttttagtaa acctgagttg cttaacaaag ccttagcggg cgaggcggta agcctgaaac
     1321 tggtatccgt cgggttactc accgcgtcga atattttcgg caaagaggga acgatggtcg
     1381 aggaccaaat gcgcgcatgg caatcgttga cccagccggg aaaaatgatt catttaaaaa
     1441 tccgcaataa agatggcgat ctacagacgg taaaaataaa accggacgtc gccgcattta
     1501 atgtgggtgt taatgagctg gcgctcaagc tcggctttgg ccttaaggca tcggatagct
     1561 ataatgccga ggcgctacat cagttattag gcaatgattt acgccctgaa gccagaccag
     1621 gtggctgggt tggcgaatgg ctggcgcaat acccggataa ttatgaggtc gtcaatacat
     1681 tagcgcgcca gattaaggat atatggaaaa ataaccaaca tcataaagat ggcggcgaac
     1741 cctataaact cgcacaacgc cttgccatgt tagcccatga aattgacgcg gtacccgcct
     1801 ggaattcgaa aagcggcaaa gatcgtacag ggatgatgga ttcagaaatc aagcgagaga
     1861 tcatttcctt acatcagacc catatgttaa gtgcgcctgg tagtcttccg gatagcggtg
     1921 gacagaaaat tttccaaaaa gtattactga atagcggtaa cctggagatt cagaaacaaa
     1981 atacgggcgg ggcgggaaac aaagtaatga aaaatttatc gccagaggtg ctcaatcttt
     2041 cctatcaaaa acgagttggg gatgaaaata tttggcagtc agtaaaaggc atttcttcat
     2101 taatcacatc ttgagtcttg aggtaactat atggaaagtc tattaaatcg tttatatgac
     2161 gcgttaggcc tggatgcgcc agaagatgag ccactgctta tcattgatga tgggatacag
     2221 gtttatttta atgaatccga tcatacactg gaaatgtgct gtccctttat gccattgcct
     2281 gacgacatcc tgactttgca gcatttttta cgtctgaact acaccagcgc cgtcactatc
     2341 ggcgctgacg cagacaatac tgctttagtg gcgctttatc gcttgccgca aaccagtacc
     2401 gaagaagagg cgctcactgg ttttgaatta ttcatttcaa acgtgaagca attgaaagag
     2461 cattatgcat aatttaatac gtcaacatac tttcttaatg agctagagcg gccgccaccg
     2521 cggtggagct ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca
     2581 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     2641 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     2701 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     2761 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     2821 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     2881 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agatctctac
     2941 gggtcggatt tgaagtcgtc ttggtaggag gcagcctgaa tggcgaatgc cgatgccctt
     3001 gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc
     3061 acttatgact gtcttcttta tcatgcaact cgtaggacag ggtgccggca gcgctctggg
     3121 tcattttcgg cgaggaccgc tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg
     3181 tattcggaat cttgcacgcc ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt
     3241 tcggcgagaa gcaggccatt atcgccggca tggcggccga cgcgctgggc tacgtcttgc
     3301 tggcgttcgc gacgcgaggc tggatggcct tccccattat gattcttctc gcttccggcg
     3361 gcatcgggat gcccgcgttg caggccatgc tgtccaggca ggtagatgac gaccatcagg
     3421 gacagcttca aggatcgctc gcggctctta ccagcctaac ttcgatcatt ggaccgctga
     3481 tcgtcacggc gatttatgcc gcctcggcga gcacatggaa cgggttggca tggattgtag
     3541 gcgccgccct ataccttgtc tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca
     3601 cctcgacctg aatggaagcc ggcggcacct cgctaacgga ttcaccactc cgcagacccg
     3661 ccataaaacg ccctgagaag cccgtgacgg gcttttcttg tattatgggt agtttccttg
     3721 catgaatcca taaaaggcgc ctgtagtgcc atttaccccc attcactgcc agagccgtga
     3781 gcgcagcgaa ctgaatgtca cgaaaaagac agcgactcag gtgcctgatg gtcggagaca
     3841 aaaggaatat tcagcgattt gcccgagctt gcgagggtgc tacttaagcc tttagggttt
     3901 taaggtctgt tttgtagagg agcaaacagc gtttgcgaca tccttttgta atactgcgga
     3961 actgactaaa gtagtgagtt atacacaggg ctgggatcta ttctttttat ctttttttat
     4021 tctttcttta ttctataaat tataaccact tgaatataaa caaaaaaaac acacaaaggt
     4081 ctagcggaat ttacagaggg tctagcagaa tttacaagtt ttccagcaaa ggtctagcag
     4141 aatttacaga tacccacaac tcaaaggaaa aggactagta attatcattg actagcccat
     4201 ctcaattggt atagtgatta aaatcaccta gaccaattga gatgtatgtc tgaattagtt
     4261 gttttcaaag caaatgaact agcgattagt cgctatgact taacggagca tgaaaccaag
     4321 ctaattttat gctgtgtggc actactcaac cccacgattg aaaaccctac aaggaaagaa
     4381 cggacggtat cgttcactta taaccaatac gctcagatga tgaacatcag tagggaaaat
     4441 gcttatggtg tattagctaa agcaaccaga gagctgatga cgagaactgt ggaaatcagg
     4501 aatcctttgg ttaaaggctt tgagattttc cagtggacaa actatgccaa gttctcaagc
     4561 gaaaaattag aattagtttt tagtgaagag atattgcctt atcttttcca gttaaaaaaa
     4621 ttcataaaat ataatctgga acatgttaag tcttttgaaa acaaatactc tatgaggatt
     4681 tatgagtggt tattaaaaga actaacacaa aagaaaactc acaaggcaaa tatagagatt
     4741 agccttgatg aatttaagtt catgttaatg cttgaaaata actaccatga gtttaaaagg
     4801 cttaaccaat gggttttgaa accaataagt aaagatttaa acacttacag caatatgaaa
     4861 ttggtggttg ataagcgagg ccgcccgact gatacgttga ttttccaagt tgaactagat
     4921 agacaaatgg atctcgtaac cgaacttgag aacaaccaga taaaaatgaa tggtgacaaa
     4981 ataccaacaa ccattacatc agattcctac ctacataacg gactaagaaa aacactacac
     5041 gatgctttaa ctgcaaaaat tcagctcacc agttttgagg caaaattttt gagtgacatg
     5101 caaagtaagt atgatctcaa tggttcgttc tcatggctca cgcaaaaaca acgaaccaca
     5161 ctagagaaca tactggctaa atacggaagg atctgaggtt cttatggctc ttgtatctat
     5221 cagtgaagca tcaagactaa caaacaaaag tagaacaact gttcaccgtt acatatcaaa
     5281 gggaaaactg tccatatgca cagatgaaaa cggtgtaaaa aagatagata catcagagct
     5341 tttacgagtt tttggtgcat tcaaagctgt tcaccatgaa cagatcgaca atgtaacaga
     5401 tgaacagcat gtaacaccta atagaacagg tgaaaccagt aaaacaaagc aactagaaca
     5461 tgaaattgaa cacctgagac aacttgttac agctcaacag tcacacatag acagcctgaa
     5521 acaggcgatg ctgcttatcg aatcaaagct gccgacaaca cgggagccag tgacgcctcc
     5581 cgtggggaaa aaatcatggc aattctggaa gaaatagcgc tttcagccgg caaaccggct
     5641 gaagccggat ctgcgattct gataacaaac tagcaacacc agaacagccc gtttgcgggc
     5701 agcaaaaccc gtacttttgg acgttccggc ggttttttgt ggcgagtggt gttcgggcgg
     5761 tgcgcgcaag atccattatg ttaaacgggc gagtttacat ctcaaaaccg cccgcttaac
     5821 accatcagaa atcctcagcg cgattttaag caccaacccc cccccgtaac acccaaatcc
     5881 atactgaaag tggctttgtt gaataaatca gatttcgggt aagtctcccc cgtagcgggt
     5941 tgtgttttca ggcaatacgc acgctttcag gcatacctgc tttcgtcatt ttgttcagcg
     6001 ctcgtaccag ggccatagcc tccgcaacct gaccatcgta gtcacgcagc gtcagtgaac
     6061 ccccgaacag agatctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     6121 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     6181 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     6241 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     6301 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     6361 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     6421 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     6481 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     6541 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc agcgatcaag
     6601 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     6661 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     6721 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     6781 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     6841 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     6901 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     6961 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     7021 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     7081 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     7141 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     7201 gccacctaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
     7261 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
     7321 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
     7381 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
     7441 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
     7501 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
     7561 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
     7621 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc gccattcagg
     7681 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
     7741 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
     7801 cgttgtaaaa cgacggccag tgagcgcgcg taatacgact cactataggg cgaattgggt
     7861 accgggcccc ccct
//
