LOCUS       sequence-215702-        5096 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ProC driven gfpmut3 expression with synTT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5096)
  AUTHORS   Cooper KG, Chong A, Starr T, Finn CE, Steele-Mortimer O
  TITLE     Predictable, Tunable Protein Production in Salmonella for Studying 
            Host-Pathogen Interactions.
  JOURNAL   Front Cell Infect Microbiol. 2017 Nov 16;7:475. doi: 
            10.3389/fcimb.2017.00475. eCollection 2017.
  PUBMED    29201859
REFERENCE   2  (bases 1 to 5096)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Cell 
            Infect Microbiol."; date: "2017-11-16"; volume: "7:475. doi"; pages:
            " 10.3389/fcimb.2017.00475. eCollection 2017"
COMMENT     Sequence Label: pCON1-ProC.gfp
FEATURES             Location/Qualifiers
     source          1..5096
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             208..921
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(238..266)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     861..881
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      961..1032
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1048..1075
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     oriT            1689..1776
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     primer_bind     complement(3089..3106)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3260..3848)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3340..3359)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4019..4810,4811..4879))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4642..4661
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4880..4984)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5052..5070
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ggtacccggg cacagctaac accacgtcgt ccctatctgc tgccctaggt ctatgagtgg
       61 ttgctggata actttacggg catgcataag gctcgtatga tatattcagg gagaccacaa
      121 cggtttccct ctacaaataa ttttgtttaa cttttactag agtcacacag gaaagtacta
      181 gtctagattt aagaaggaga tatacatatg agtaaaggag aagaactttt cactggagtt
      241 gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga
      301 gagggtgaag gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga
      361 aaactacctg ttccatggcc aacacttgtc actactttcg gttatggtgt tcaatgcttt
      421 gcgagatacc cagatcatat gaaacagcat gactttttca agagtgccat gcccgaaggt
      481 tatgtacagg aaagaactat atttttcaaa gatgacggga actacaagac acgtgctgaa
      541 gtcaagtttg aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa
      601 gaagatggaa acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac
      661 atcatggcag acaaacaaaa gaatggaatc aaagttaact tcaaaattag acacaacatt
      721 gaagatggaa gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc
      781 cctgtccttt taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc
      841 aacgaaaaga gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat
      901 ggcatggatg aactatacaa ataaatgtcc agacctgcag gcatgcaagc ttccaggcat
      961 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
     1021 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat
     1081 aaagctttaa tgcggtagtt tatcacagtt aaattgctaa cgcagtcagg caccgtgtat
     1141 gaaatctaac aatgcgctca tcgtcatcct cggcaccgtc accctggatg ctgtaggcat
     1201 aggcttggtt atgccggtac tgccgggcct cttgcgggat atcgtccatt ccgacagcat
     1261 cgccagtcac tatggcgtgc tgctagcgct tttagccgct ttagcggcct ttccccctac
     1321 ccgaagggtg ggggcgcgtg tgcagccccg cagggcctgt ctcggtcgat cattcagccc
     1381 ggctcatcct tctggcgtgg cggcagaccg aacaaggcgc ggtcgtggtc gcgttcaagg
     1441 tacgcatcca ttgccgccat gagccgatcc tccggccact cgctgctgtt caccttggcc
     1501 aaaatcatgg cccccaccag caccttgcgc cttgtttcgt tcttgcgctc ttgctgctgt
     1561 tcccttgccc gcacccgctg aatttcggca ttgattcgcg ctcgttgttc ttcgagcttg
     1621 gccagccgat ccgccgcctt gttgctcccc ttaaccatct tgacacccca ttgttaatgt
     1681 gctgtctcgt aggctatcat ggaggcacag cggcggcaat cccgacccta ctttgtaggg
     1741 gagggcgcac ttaccggttt ctcttcgaga aactggccta acggccaccc ttcgggcggt
     1801 gcgctctccg agggccattg catggagccg aaaagcaaaa gcaacagcga ggcagcatgg
     1861 cgatttatca ccttacggcg aaaaccggca gcaggtcggg cggccaatcg gccagggcca
     1921 aggccgacta catccagcgc gaaggcaagt atgcccgcga catggatgaa gtcttgcacg
     1981 ccgaatccgg gcacatgccg gagttcgtcg agcggcccgc cgactactgg gatgctgccg
     2041 acctgtatga acgcgccaat gggcggctgt tcaaggaggt cgaatttgcc ctgccggtcg
     2101 agctgaccct cgaccagcag aaggcgctgg cgtccgagtt cgcccagcac ctgaccggtg
     2161 ccgagcgcct gccgtatacg ctggccatcc atgccggtgg cggcgagaac ccgcactgcc
     2221 acctgatgat ctccgagcgg atcaatgacg gcatcgagcg gcccgccgct cagtggttca
     2281 agcggtacaa cggcaagacc ccggagaagg gcggggcaca gaagaccgaa gcgctcaagc
     2341 ccaaggcatg gcttgagcag acccgcgagg catgggccga ccatgccaac cgggcattag
     2401 agcgggctgg ccacgacgcc cgcattgacc acagaacact tgaggcgcag ggcatcgagc
     2461 gcctgcccgg tgttcacctg gggccgaacg tggtggagat ggaaggccgg ggcatccgca
     2521 ccgaccgggc agacgtggcc ctgaacatcg acaccgccaa cgcccagatc atcgacttac
     2581 aggaataccg ggaggcaata gaccatgaac gcaatcgaca gagtgaagaa atccagaggc
     2641 atcaacgagt tagcggagca gatcgaaccg ctggcccaga gcatggcgac actggccgac
     2701 gaagcccggc aggtcatgag ccagacccag caggccagcg aggcgcaggc ggcggagtgg
     2761 ctgaaagccc agcgccagac aggggcggca tgggtggagc tggccaaaga gttgcgggag
     2821 gtagccgccg aggtgagcag cgccgcgcag agcgcccgga gcgcgtcgcg ggggtggcac
     2881 tggaagctat ggctaaccgt gatgctggct tccatgatgc ctacggtggt gctgctgatc
     2941 gcatcgttgc tcttgctcga cctgacgcca ctgacaaccg aggacggctc gatctggctg
     3001 cgcttggtgg cccgatgaag aacgacagga ctttgcaggc cataggccga cagctcaagg
     3061 ccatgggctg tgagcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     3121 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     3181 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     3241 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     3301 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     3361 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     3421 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     3481 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     3541 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     3601 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     3661 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     3721 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     3781 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     3841 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     3901 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     3961 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     4021 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     4081 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     4141 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     4201 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     4261 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     4321 ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     4381 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     4441 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     4501 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     4561 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     4621 cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca
     4681 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     4741 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     4801 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     4861 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     4921 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     4981 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     5041 taacctataa aaataggcgt atcacgaggc cctttcgtct tcaagaattc gagctc
//
