LOCUS       sequence-215708-        5074 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ProA driven mCherry expression with synTT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5074)
  AUTHORS   Cooper KG, Chong A, Starr T, Finn CE, Steele-Mortimer O
  TITLE     Predictable, Tunable Protein Production in Salmonella for Studying 
            Host-Pathogen Interactions.
  JOURNAL   Front Cell Infect Microbiol. 2017 Nov 16;7:475. doi: 
            10.3389/fcimb.2017.00475. eCollection 2017.
  PUBMED    29201859
REFERENCE   2  (bases 1 to 5074)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Cell 
            Infect Microbiol."; date: "2017-11-16"; volume: "7:475. doi"; pages:
            " 10.3389/fcimb.2017.00475. eCollection 2017"
COMMENT     Sequence Label: pCON1-ProA.mCherry
FEATURES             Location/Qualifiers
     source          1..5074
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             217..927
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(362..380)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     630..649
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      948..1019
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1035..1062
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     oriT            1676..1763
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     primer_bind     complement(3076..3093)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3247..3835)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3327..3346)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4006..4797,4798..4866))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4629..4648
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4867..4971)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5039..5057
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ttcgagctcg gtacccgggc acagctaaca ccacgtcgtc cctatctgct gccctaggtc
       61 tatgagtggt tgctggataa ctttacgggc atgcataagg ctcgtaggct atattcaggg
      121 agaccacaac ggtttccctc tacaaataat tttgtttaac ttttactaga gtcacacagg
      181 aaagtactag tctagattta agaaggagat atacatatgg tgagcaaggg cgaggaggat
      241 aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac
      301 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
      361 gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct
      421 cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg
      481 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc
      541 gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag
      601 ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg
      661 gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag
      721 aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc
      781 aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc
      841 cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc
      901 ggcggcatgg acgagctgta caagtaagca tgcaagcttc caggcatcaa ataaaacgaa
      961 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
     1021 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttataaa gctttaatgc
     1081 ggtagtttat cacagttaaa ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat
     1141 gcgctcatcg tcatcctcgg caccgtcacc ctggatgctg taggcatagg cttggttatg
     1201 ccggtactgc cgggcctctt gcgggatatc gtccattccg acagcatcgc cagtcactat
     1261 ggcgtgctgc tagcgctttt agccgcttta gcggcctttc cccctacccg aagggtgggg
     1321 gcgcgtgtgc agccccgcag ggcctgtctc ggtcgatcat tcagcccggc tcatccttct
     1381 ggcgtggcgg cagaccgaac aaggcgcggt cgtggtcgcg ttcaaggtac gcatccattg
     1441 ccgccatgag ccgatcctcc ggccactcgc tgctgttcac cttggccaaa atcatggccc
     1501 ccaccagcac cttgcgcctt gtttcgttct tgcgctcttg ctgctgttcc cttgcccgca
     1561 cccgctgaat ttcggcattg attcgcgctc gttgttcttc gagcttggcc agccgatccg
     1621 ccgccttgtt gctcccctta accatcttga caccccattg ttaatgtgct gtctcgtagg
     1681 ctatcatgga ggcacagcgg cggcaatccc gaccctactt tgtaggggag ggcgcactta
     1741 ccggtttctc ttcgagaaac tggcctaacg gccacccttc gggcggtgcg ctctccgagg
     1801 gccattgcat ggagccgaaa agcaaaagca acagcgaggc agcatggcga tttatcacct
     1861 tacggcgaaa accggcagca ggtcgggcgg ccaatcggcc agggccaagg ccgactacat
     1921 ccagcgcgaa ggcaagtatg cccgcgacat ggatgaagtc ttgcacgccg aatccgggca
     1981 catgccggag ttcgtcgagc ggcccgccga ctactgggat gctgccgacc tgtatgaacg
     2041 cgccaatggg cggctgttca aggaggtcga atttgccctg ccggtcgagc tgaccctcga
     2101 ccagcagaag gcgctggcgt ccgagttcgc ccagcacctg accggtgccg agcgcctgcc
     2161 gtatacgctg gccatccatg ccggtggcgg cgagaacccg cactgccacc tgatgatctc
     2221 cgagcggatc aatgacggca tcgagcggcc cgccgctcag tggttcaagc ggtacaacgg
     2281 caagaccccg gagaagggcg gggcacagaa gaccgaagcg ctcaagccca aggcatggct
     2341 tgagcagacc cgcgaggcat gggccgacca tgccaaccgg gcattagagc gggctggcca
     2401 cgacgcccgc attgaccaca gaacacttga ggcgcagggc atcgagcgcc tgcccggtgt
     2461 tcacctgggg ccgaacgtgg tggagatgga aggccggggc atccgcaccg accgggcaga
     2521 cgtggccctg aacatcgaca ccgccaacgc ccagatcatc gacttacagg aataccggga
     2581 ggcaatagac catgaacgca atcgacagag tgaagaaatc cagaggcatc aacgagttag
     2641 cggagcagat cgaaccgctg gcccagagca tggcgacact ggccgacgaa gcccggcagg
     2701 tcatgagcca gacccagcag gccagcgagg cgcaggcggc ggagtggctg aaagcccagc
     2761 gccagacagg ggcggcatgg gtggagctgg ccaaagagtt gcgggaggta gccgccgagg
     2821 tgagcagcgc cgcgcagagc gcccggagcg cgtcgcgggg gtggcactgg aagctatggc
     2881 taaccgtgat gctggcttcc atgatgccta cggtggtgct gctgatcgca tcgttgctct
     2941 tgctcgacct gacgccactg acaaccgagg acggctcgat ctggctgcgc ttggtggccc
     3001 gatgaagaac gacaggactt tgcaggccat aggccgacag ctcaaggcca tgggctgtga
     3061 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     3121 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     3181 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     3241 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     3301 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     3361 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     3421 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     3481 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     3541 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     3601 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     3661 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     3721 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     3781 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     3841 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     3901 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     3961 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     4021 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     4081 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     4141 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     4201 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     4261 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     4321 tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     4381 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     4441 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     4501 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     4561 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     4621 aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     4681 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     4741 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     4801 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     4861 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     4921 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     4981 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     5041 taggcgtatc acgaggccct ttcgtcttca agaa
//
