LOCUS       sequence-215714-        2371 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICSL50010
FEATURES             Location/Qualifiers
     source          1..2371
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             151..942
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1113..1701
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1602..1621
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1855..1872
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             1951..2040
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /label=3xMyc
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
     CDS             1981..2070
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /label=3xMyc
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
     primer_bind     complement(2110..2128)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2196..2300
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggcttgttat gactgttttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa
       61 gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag
      121 ggcagtcgcc ctaaaacaaa gttaaacatc atgagggaag cggtgatcgc cgaagtatcg
      181 actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc
      241 gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagcga tattgatttg
      301 ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt
      361 ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt
      421 gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga
      481 gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat
      541 ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg
      601 gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta
      661 acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg
      721 tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac
      781 tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct
      841 tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc
      901 cattacgtaa aaggcgagat caccaaggta gtcggcaaat aactgtcaga ccaagtttac
      961 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     1021 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     1081 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     1141 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     1201 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     1261 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     1321 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     1381 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     1441 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     1501 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     1561 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     1621 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     1681 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     1741 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     1801 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     1861 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     1921 ccgattcatt aatcactgtg gtctcattcg gaacaaaagt tgatctctga agaggacctt
     1981 gagcagaaat tgatctctga ggaagatctt gagcagaagc ttatctcaga ggaggatttg
     2041 gagcaaaaac ttatttctga agaggacctt tgagctttga gaccacgtgg ctcttcagtg
     2101 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     2161 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     2221 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     2281 aatattgaaa aaggaagagt atgcgctcac gcaactggtc cagaaccttg accgaacgca
     2341 gcggtggtaa cggcgcagtg gcggttttca t
//
