LOCUS       sequence-215721-        2516 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICH41421
FEATURES             Location/Qualifiers
     source          1..2516
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      718..1306
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1207..1226
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1460..1477
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      1566..1818
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(1860..1878)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1946..2050
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2272..2516,1..547)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc
       61 agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt
      121 cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag
      181 cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct
      241 atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga
      301 tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat
      361 cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc
      421 cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg
      481 cgcagatcag ttggaagaat ttgtccatta cgtaaaaggc gagatcacca aggtagtcgg
      541 caaataactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
      601 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
      661 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
      721 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
      781 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
      841 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
      901 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
      961 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     1021 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     1081 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     1141 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     1201 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     1261 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     1321 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     1381 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     1441 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     1501 caaaccgcct ctccccgcgc gttggccgat tcattaatca ctctgtggtc tcagcttgtc
     1561 aagcagatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc
     1621 ttgcgatgat tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt
     1681 aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt
     1741 aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt
     1801 catctatgtt actagatcga cgcttgagac cacgaagtgg ctcttcagtg gacgaaaggg
     1861 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     1921 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     1981 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     2041 aaggaagagt atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa
     2101 cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttttggggt acagtctatg
     2161 cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc
     2221 agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat catgagggaa
     2281 gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat
     2341 ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag
     2401 ccacacagcg atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg
     2461 cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag cgagat
//
