LOCUS       sequence-215723-        5096 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ProB driven gfpmut3 expression with synTT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5096)
  AUTHORS   Cooper KG, Chong A, Starr T, Finn CE, Steele-Mortimer O
  TITLE     Predictable, Tunable Protein Production in Salmonella for Studying 
            Host-Pathogen Interactions.
  JOURNAL   Front Cell Infect Microbiol. 2017 Nov 16;7:475. doi: 
            10.3389/fcimb.2017.00475. eCollection 2017.
  PUBMED    29201859
REFERENCE   2  (bases 1 to 5096)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Cell 
            Infect Microbiol."; date: "2017-11-16"; volume: "7:475. doi"; pages:
            " 10.3389/fcimb.2017.00475. eCollection 2017"
COMMENT     Sequence Label: pCON1-ProB.gfp
FEATURES             Location/Qualifiers
     source          1..5096
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     50..68
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             302..1015
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(332..360)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     955..975
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      1055..1126
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1142..1169
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     oriT            1783..1870
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     primer_bind     complement(3183..3200)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3354..3942)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3434..3453)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4113..4904,4905..4973))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4736..4755
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4974..5078)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
       61 cacgaggccc tttcgtcttc aagaattcga gctcggtacc cgggcacagc taacaccacg
      121 tcgtccctat ctgctgccct aggtctatga gtggttgctg gataacttta cgggcatgca
      181 taaggctcgt aatatatatt cagggagacc acaacggttt ccctctacaa ataattttgt
      241 ttaactttta ctagagtcac acaggaaagt actagtctag atttaagaag gagatataca
      301 tatgagtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg
      361 tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg
      421 aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact
      481 tgtcactact ttcggttatg gtgttcaatg ctttgcgaga tacccagatc atatgaaaca
      541 gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt
      601 caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt
      661 taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa
      721 attggaatac aactataact cacacaatgt atacatcatg gcagacaaac aaaagaatgg
      781 aatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggaagcgttc aactagcaga
      841 ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta
      901 cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct
      961 tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat acaaataaat
     1021 gtccagacct gcaggcatgc aagcttccag gcatcaaata aaacgaaagg ctcagtcgaa
     1081 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
     1141 tggctcacct tcgggtgggc ctttctgcgt ttataaagct ttaatgcggt agtttatcac
     1201 agttaaattg ctaacgcagt caggcaccgt gtatgaaatc taacaatgcg ctcatcgtca
     1261 tcctcggcac cgtcaccctg gatgctgtag gcataggctt ggttatgccg gtactgccgg
     1321 gcctcttgcg ggatatcgtc cattccgaca gcatcgccag tcactatggc gtgctgctag
     1381 cgcttttagc cgctttagcg gcctttcccc ctacccgaag ggtgggggcg cgtgtgcagc
     1441 cccgcagggc ctgtctcggt cgatcattca gcccggctca tccttctggc gtggcggcag
     1501 accgaacaag gcgcggtcgt ggtcgcgttc aaggtacgca tccattgccg ccatgagccg
     1561 atcctccggc cactcgctgc tgttcacctt ggccaaaatc atggccccca ccagcacctt
     1621 gcgccttgtt tcgttcttgc gctcttgctg ctgttccctt gcccgcaccc gctgaatttc
     1681 ggcattgatt cgcgctcgtt gttcttcgag cttggccagc cgatccgccg ccttgttgct
     1741 ccccttaacc atcttgacac cccattgtta atgtgctgtc tcgtaggcta tcatggaggc
     1801 acagcggcgg caatcccgac cctactttgt aggggagggc gcacttaccg gtttctcttc
     1861 gagaaactgg cctaacggcc acccttcggg cggtgcgctc tccgagggcc attgcatgga
     1921 gccgaaaagc aaaagcaaca gcgaggcagc atggcgattt atcaccttac ggcgaaaacc
     1981 ggcagcaggt cgggcggcca atcggccagg gccaaggccg actacatcca gcgcgaaggc
     2041 aagtatgccc gcgacatgga tgaagtcttg cacgccgaat ccgggcacat gccggagttc
     2101 gtcgagcggc ccgccgacta ctgggatgct gccgacctgt atgaacgcgc caatgggcgg
     2161 ctgttcaagg aggtcgaatt tgccctgccg gtcgagctga ccctcgacca gcagaaggcg
     2221 ctggcgtccg agttcgccca gcacctgacc ggtgccgagc gcctgccgta tacgctggcc
     2281 atccatgccg gtggcggcga gaacccgcac tgccacctga tgatctccga gcggatcaat
     2341 gacggcatcg agcggcccgc cgctcagtgg ttcaagcggt acaacggcaa gaccccggag
     2401 aagggcgggg cacagaagac cgaagcgctc aagcccaagg catggcttga gcagacccgc
     2461 gaggcatggg ccgaccatgc caaccgggca ttagagcggg ctggccacga cgcccgcatt
     2521 gaccacagaa cacttgaggc gcagggcatc gagcgcctgc ccggtgttca cctggggccg
     2581 aacgtggtgg agatggaagg ccggggcatc cgcaccgacc gggcagacgt ggccctgaac
     2641 atcgacaccg ccaacgccca gatcatcgac ttacaggaat accgggaggc aatagaccat
     2701 gaacgcaatc gacagagtga agaaatccag aggcatcaac gagttagcgg agcagatcga
     2761 accgctggcc cagagcatgg cgacactggc cgacgaagcc cggcaggtca tgagccagac
     2821 ccagcaggcc agcgaggcgc aggcggcgga gtggctgaaa gcccagcgcc agacaggggc
     2881 ggcatgggtg gagctggcca aagagttgcg ggaggtagcc gccgaggtga gcagcgccgc
     2941 gcagagcgcc cggagcgcgt cgcgggggtg gcactggaag ctatggctaa ccgtgatgct
     3001 ggcttccatg atgcctacgg tggtgctgct gatcgcatcg ttgctcttgc tcgacctgac
     3061 gccactgaca accgaggacg gctcgatctg gctgcgcttg gtggcccgat gaagaacgac
     3121 aggactttgc aggccatagg ccgacagctc aaggccatgg gctgtgagcg ctcttccgct
     3181 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     3241 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     3301 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     3361 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     3421 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     3481 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     3541 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     3601 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     3661 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     3721 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     3781 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     3841 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     3901 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     3961 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     4021 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     4081 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     4141 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     4201 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     4261 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     4321 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     4381 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg
     4441 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     4501 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     4561 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     4621 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     4681 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat
     4741 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     4801 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     4861 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     4921 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     4981 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     5041 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagt
//
