LOCUS       sequence-215725-        7875 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PsopB driven sopB and sigE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7875)
  AUTHORS   Knodler LA, Winfree S, Drecktrah D, Ireland R, Steele-Mortimer O
  TITLE     Ubiquitination of the bacterial inositol phosphatase, SopB, 
            regulates its biological activity at the plasma membrane.
  JOURNAL   Cell Microbiol. 2009 Nov;11(11):1652-70. doi: 
            10.1111/j.1462-5822.2009.01356.x. Epub 2009 Jul 13.
  PUBMED    19614667
REFERENCE   2  (bases 1 to 7875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1462-5822.2009.01356.x"; journalName: "Cell Microbiol"; 
            date: "2009-11"; volume: "11"; issue: "11"; pages: "1652-70"
COMMENT     Sequence Label: pSopBWT
FEATURES             Location/Qualifiers
     source          1..7875
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2528..2548)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2528..2546)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2567..2583)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2567..2583)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2580..2602)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2591..2607
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2615..2621,2622..2639,2640..2645))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2660..2681
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2798..2815)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      3960..4182
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             4230..5180
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     CDS             complement(join(6201..6992,6993..7061))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6824..6843
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7062..7166)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7192..7647)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7329..7350)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7541..7560
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7774..7796
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7788..7805
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7789..7805
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7815..7834
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7815..7833
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     7858..7874
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     7859..7875
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ataagctttc tgttcaagca tggaatagga aaaacgaata ttcttcgtca cggtcttact
       61 tgtccggggc tttgctggca tacacacacc tgtataacat ttgatgtaac gccgttactt
      121 tacgcaggag taaatcggtg aatttgatct gagtcaagaa ggtgggtttt caataaaagt
      181 tgtgccataa attgtgaagt ttgtagattt tatgaacatt tgatgtaccg atctccccca
      241 tgatcgccac tacgtatgga cgtcaggatg cctccccgcc tgatcagaag cgtttcctca
      301 ttaaaaagga cattttttta aagttcctgg tgcataaaag tcacatcctt ttaaagggtt
      361 gttaaccctg ttgaatgttc ccactcccct attcaggaat attaaaaacg ctatgcaaat
      421 acagagcttc tatcactcag cttcactaaa aacccaggag gcttttaaaa gcctacaaaa
      481 aaccttatac aacggaatgc agattctctc aggccagggc aaagcgccgg ctaaagcgcc
      541 cgacgctcgc ccggaaatta ttgtcctgcg agaacccggc gcgacatggg ggaattatct
      601 acagcatcag aaggcgtcta accactcgct gcataacctc tataacttac agcgcgatct
      661 tcttaccgtc gcggcaaccg ttctgggtaa acaagacccg gttctaacgt caatggcaaa
      721 ccaaatggag ttagccaaag ttaaagcgga ccggccagca acaaaacaag aagaagccgc
      781 ggcaaaagca ttgaagaaaa atcttatcga acttattgca gcacgcactc agcagcagga
      841 tggcttacct gcaaaagaag ctcatcgctt tgcggcagta gcgtttagag atgctcaggt
      901 caagcagctt aataaccagc cctggcaaac cataaaaaat acactcacgc ataacgggca
      961 tcactatacc aacacgcagc tccctgcagc agagatgaaa atcggcgcaa aagatatctt
     1021 tcccagtgct tatgagggaa agggcgtatg cagttgggat accaagaata ttcatcacgc
     1081 caataatttg tggatgtcca cggtgagtgt gcatgaggac ggtaaagata aaacgctttt
     1141 ttgcgggata cgtcatggcg tgctttcccc ctatcatgaa aaagatccgc ttctgcgtca
     1201 cgtcggcgct gaaaacaaag ccaaagaagt attaactgcg gcacttttta gtaaacctga
     1261 gttgcttaac aaagccttag cgggcgaggc ggtaagcctg aaactggtat ccgtcgggtt
     1321 actcaccgcg tcgaatattt tcggcaaaga gggaacgatg gtcgaggacc aaatgcgcgc
     1381 atggcaatcg ttgacccagc cgggaaaaat gattcattta aaaatccgca ataaagatgg
     1441 cgatctacag acggtaaaaa taaaaccgga cgtcgccgca tttaatgtgg gtgttaatga
     1501 gctggcgctc aagctcggct ttggccttaa ggcatcggat agctataatg ccgaggcgct
     1561 acatcagtta ttaggcaatg atttacgccc tgaagccaga ccaggtggct gggttggcga
     1621 atggctggcg caatacccgg ataattatga ggtcgtcaat acattagcgc gccagattaa
     1681 ggatatatgg aaaaataacc aacatcataa agatggcggc gaaccctata aactcgcaca
     1741 acgccttgcc atgttagccc atgaaattga cgcggtaccc gcctggaatt gtaaaagcgg
     1801 caaagatcgt acagggatga tggattcaga aatcaagcga gagatcattt ccttacatca
     1861 gacccatatg ttaagtgcgc ctggtagtct tccggatagc ggtggacaga aaattttcca
     1921 aaaagtatta ctgaatagcg gtaacctgga gattcagaaa caaaatacgg gcggggcggg
     1981 aaacaaagta atgaaaaatt tatcgccaga ggtgctcaat ctttcctatc aaaaacgagt
     2041 tggggatgaa aatatttggc agtcagtaaa aggcatttct tcattaatca catcttgagt
     2101 cttgaggtaa ctatatggaa agtctattaa atcgtttata tgacgcgtta ggcctggatg
     2161 cgccagaaga tgagccactg cttatcattg atgatgggat acaggtttat tttaatgaat
     2221 ccgatcatac actggaaatg tgctgtccct ttatgccatt gcctgacgac atcctgactt
     2281 tgcagcattt tttacgtctg aactacacca gcgccgtcac tatcggcgct gacgcagaca
     2341 atactgcttt agtggcgctt tatcgcttgc cgcaaaccag taccgaagaa gaggcgctca
     2401 ctggttttga attattcatt tcaaacgtga agcaattgaa agagcattat gcataattta
     2461 atacgtcaac atactttctt aatgagctag agcggccgcc accgcggtgg agctccagct
     2521 tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc
     2581 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     2641 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     2701 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     2761 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     2821 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     2881 cagaatcagg ggataacgca ggaaagaaca tgtgagatct ctacgggtcg gatttgaagt
     2941 cgtcttggta ggaggcagcc tgaatggcga atgccgatgc ccttgagagc cttcaaccca
     3001 gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat gactgtcttc
     3061 tttatcatgc aactcgtagg acagggtgcc ggcagcgctc tgggtcattt tcggcgagga
     3121 ccgctttcgc tggagcgcga cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca
     3181 cgccctcgct caagccttcg tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc
     3241 cattatcgcc ggcatggcgg ccgacgcgct gggctacgtc ttgctggcgt tcgcgacgcg
     3301 aggctggatg gccttcccca ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc
     3361 gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat cagggacagc ttcaaggatc
     3421 gctcgcggct cttaccagcc taacttcgat cattggaccg ctgatcgtca cggcgattta
     3481 tgccgcctcg gcgagcacat ggaacgggtt ggcatggatt gtaggcgccg ccctatacct
     3541 tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga cctgaatgga
     3601 agccggcggc acctcgctaa cggattcacc actccgcaga cccgccataa aacgccctga
     3661 gaagcccgtg acgggctttt cttgtattat gggtagtttc cttgcatgaa tccataaaag
     3721 gcgcctgtag tgccatttac ccccattcac tgccagagcc gtgagcgcag cgaactgaat
     3781 gtcacgaaaa agacagcgac tcaggtgcct gatggtcgga gacaaaagga atattcagcg
     3841 atttgcccga gcttgcgagg gtgctactta agcctttagg gttttaaggt ctgttttgta
     3901 gaggagcaaa cagcgtttgc gacatccttt tgtaatactg cggaactgac taaagtagtg
     3961 agttatacac agggctggga tctattcttt ttatcttttt ttattctttc tttattctat
     4021 aaattataac cacttgaata taaacaaaaa aaacacacaa aggtctagcg gaatttacag
     4081 agggtctagc agaatttaca agttttccag caaaggtcta gcagaattta cagataccca
     4141 caactcaaag gaaaaggact agtaattatc attgactagc ccatctcaat tggtatagtg
     4201 attaaaatca cctagaccaa ttgagatgta tgtctgaatt agttgttttc aaagcaaatg
     4261 aactagcgat tagtcgctat gacttaacgg agcatgaaac caagctaatt ttatgctgtg
     4321 tggcactact caaccccacg attgaaaacc ctacaaggaa agaacggacg gtatcgttca
     4381 cttataacca atacgctcag atgatgaaca tcagtaggga aaatgcttat ggtgtattag
     4441 ctaaagcaac cagagagctg atgacgagaa ctgtggaaat caggaatcct ttggttaaag
     4501 gctttgagat tttccagtgg acaaactatg ccaagttctc aagcgaaaaa ttagaattag
     4561 tttttagtga agagatattg ccttatcttt tccagttaaa aaaattcata aaatataatc
     4621 tggaacatgt taagtctttt gaaaacaaat actctatgag gatttatgag tggttattaa
     4681 aagaactaac acaaaagaaa actcacaagg caaatataga gattagcctt gatgaattta
     4741 agttcatgtt aatgcttgaa aataactacc atgagtttaa aaggcttaac caatgggttt
     4801 tgaaaccaat aagtaaagat ttaaacactt acagcaatat gaaattggtg gttgataagc
     4861 gaggccgccc gactgatacg ttgattttcc aagttgaact agatagacaa atggatctcg
     4921 taaccgaact tgagaacaac cagataaaaa tgaatggtga caaaatacca acaaccatta
     4981 catcagattc ctacctacat aacggactaa gaaaaacact acacgatgct ttaactgcaa
     5041 aaattcagct caccagtttt gaggcaaaat ttttgagtga catgcaaagt aagtatgatc
     5101 tcaatggttc gttctcatgg ctcacgcaaa aacaacgaac cacactagag aacatactgg
     5161 ctaaatacgg aaggatctga ggttcttatg gctcttgtat ctatcagtga agcatcaaga
     5221 ctaacaaaca aaagtagaac aactgttcac cgttacatat caaagggaaa actgtccata
     5281 tgcacagatg aaaacggtgt aaaaaagata gatacatcag agcttttacg agtttttggt
     5341 gcattcaaag ctgttcacca tgaacagatc gacaatgtaa cagatgaaca gcatgtaaca
     5401 cctaatagaa caggtgaaac cagtaaaaca aagcaactag aacatgaaat tgaacacctg
     5461 agacaacttg ttacagctca acagtcacac atagacagcc tgaaacaggc gatgctgctt
     5521 atcgaatcaa agctgccgac aacacgggag ccagtgacgc ctcccgtggg gaaaaaatca
     5581 tggcaattct ggaagaaata gcgctttcag ccggcaaacc ggctgaagcc ggatctgcga
     5641 ttctgataac aaactagcaa caccagaaca gcccgtttgc gggcagcaaa acccgtactt
     5701 ttggacgttc cggcggtttt ttgtggcgag tggtgttcgg gcggtgcgcg caagatccat
     5761 tatgttaaac gggcgagttt acatctcaaa accgcccgct taacaccatc agaaatcctc
     5821 agcgcgattt taagcaccaa cccccccccg taacacccaa atccatactg aaagtggctt
     5881 tgttgaataa atcagatttc gggtaagtct cccccgtagc gggttgtgtt ttcaggcaat
     5941 acgcacgctt tcaggcatac ctgctttcgt cattttgttc agcgctcgta ccagggccat
     6001 agcctccgca acctgaccat cgtagtcacg cagcgtcagt gaacccccga acagagatct
     6061 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     6121 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     6181 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     6241 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     6301 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     6361 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     6421 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     6481 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     6541 tcgtttggta tggcttcatt cagctccggt tcccagcgat caaggcgagt tacatgatcc
     6601 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     6661 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     6721 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     6781 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     6841 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     6901 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     6961 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     7021 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     7081 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     7141 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc taaattgtaa
     7201 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     7261 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     7321 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     7381 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     7441 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     7501 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     7561 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     7621 cgcttaatgc gccgctacag ggcgcgtccc attcgccatt caggctgcgc aactgttggg
     7681 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
     7741 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
     7801 ccagtgagcg cgcgtaatac gactcactat agggcgaatt gggtaccggg ccccccctcg
     7861 aggtcgacgg tatcg
//
