LOCUS       sequence-215733-        2274 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SapTrap donor vector for point mutagenesis; 
            pENTR-221::syntron(lox2272-119+).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Schwartz M, Jorgensen E
  TITLE     SapTrap vectors for introducing point mutations with unc-119+ 
            selection.
  JOURNAL   MicroPubl Biol. 2018 Jul 29;2018:10.17912/DDVH-BG64. doi: 
            10.17912/DDVH-BG64.
  PUBMED    32550388
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MicroPubl 
            Biol."; date: "2018-07-29"; volume: "2018:10.17912/DDVH-BG64. doi"; 
            pages: " 10.17912/DDVH-BG64"
COMMENT     Sequence Label: pMLS415
FEATURES             Location/Qualifiers
     source          1..2274
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    49..82
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             2041..2052
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    2201..2234
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
ORIGIN
        1 gtacaaaaaa gcaggctgct cttcgagttt aaacatgatt ttactaacat aacttcgtat
       61 aaagtatcct atacgaagtt atgacattct ctaatgaaaa aatctttcag ttgaaattga
      121 aaatgagtta aagttggagt ttttattgaa aacagatttc cgtgtgatta gtgtttttag
      181 cgagtgtgac aggacagcga aaaaatatag aaacaagggg ggaactgaaa agcttaggaa
      241 tgcattgaac atgagaaggg gaaggggaag gaacaaacta gacaggaatt attggaattt
      301 aatcacattt ggagtttttt ttctattcga cagaataatt atccagaaca tttttgtatt
      361 aaatatttat gcatcatatg agtagtcggc tttgttgtgc atgacgagtt tgttatcgac
      421 gaaatagaag ctgtcagaac gagtctcgtt tggattgttg atcatgtcgt ccactgaaaa
      481 agagattagt cttttgaatt gtacttttta gaataatgac tcactgagtt gttgagagag
      541 ttgagggaac tcatagatat gttcacagtt gtttcgtgaa ttcggaatac agaatccgaa
      601 ttcaaagtca aaacacttca gaaggcgatc tttgaagaag tgacgttcga tcattcggaa
      661 atgatggatt gggatgtctc ctactttgaa ttccacagtt gctccgaccg ttttgagttt
      721 caaaaagttt ggagcaaatc tataacgcac gtatcttgcc gactcctgtg gcgactcatc
      781 attctcctga tcgttctccg gtttggcgat ctcgaaaagc acttgctcag tgtccagatc
      841 acggatttgg aacttggtga actcgatgtt atagatgttc gcagatgggg agcataagaa
      901 tcctaaattt atgttttaaa ctgaaatcca aagggagcaa gataccttga gtgattcccg
      961 gaagtgctaa aacgtcgttc ggagtgattt gagctttctt cgcaagctcc gattccgttg
     1021 tgattccttg ttcggtgctt ggtggtggcc gtggcatctg gaaatatgga aaagttcaac
     1081 aaaaagaaaa gagaaaagaa tgaaatcgga tatcaagagt tagttgagcg gtttctctag
     1141 ttttctgagt ctcacctgcg acgggaaggt cgccgagccg ggtggaatcg attgttgttg
     1201 ctcggctttc atatcggttt ggttggaagc ggctgaaaac ggaaagaagt ggaagaagga
     1261 aaagagtgtg gtgtgacagg aaaatggtaa ttagagggtg ccaaataacc agctatattt
     1321 tgtttttttt tgaaaacatt tttaaaaaga aaaatacgat aatgatatca gatggatttc
     1381 cggaaaactg gtatgaaaaa tttcaacctt tttgagtaca tgtaatcaaa atacactttg
     1441 taaattatca tttttattga aactccacca tttttctatt tataacgcta ataatttgaa
     1501 aaagaaacct gttgcgaacc gcggggtgaa tcccaaaaac gaatgcgttt tggtggagtg
     1561 attgattcga atcgaagaag aaaaagaaga agacgtggaa tagagagctc actcttaacc
     1621 gagcagcaca caccgacaga aaaaaaaatg aaatgaatga gggtcttctt cttcttcttc
     1681 ttcgaatgat tgacagaaat gggaaaaaga ggaagattga gaagggaaaa aggaaggaga
     1741 aaagaagcag aagaagacgt cagagaggag aggaacgagc ggaaaagcag cgggcgcaag
     1801 tcatagaagt agcagagctg gggagaagaa gacactatcc aagaaaggaa tgacgagaga
     1861 gtatgcaaag gggtataggg tgcagacaga ataggaacag aataacagat gatgagccaa
     1921 gaagagttga aaagggcgat gaatttgtca tgtaacttaa tttgggtcaa tttgagcatg
     1981 atgaattgaa atcatccctt gttgggagtt aataaccggt ttgttatcag aaaccctgta
     2041 atagaagggc gccctaactt tgagccaatt catcccggtt tctgtcaaat atatcaaaaa
     2101 gtggtcaact gacaaattgt ttttgatatt ataataaaca ttttatccgt taacaatttt
     2161 cgaatacttt ttacaaggac ttggataaat tggctcaaag ataacttcgt ataaagtatc
     2221 ctatacgaag ttattaacta atctgattta aattttcgaa gagcacccag cttt
//
