LOCUS       sequence-215736-        8920 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral expression vector for direct cardiac reprogramming.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8920)
  AUTHORS   Wang L, Liu Z, Yin C, Asfour H, Chen O, Li Y, Bursac N, Liu J, Qian 
            L
  TITLE     Stoichiometry of Gata4, Mef2c, and Tbx5 influences the efficiency 
            and quality of induced cardiac myocyte reprogramming.
  JOURNAL   Circ Res. 2015 Jan 16;116(2):237-44. doi: 
            10.1161/CIRCRESAHA.116.305547. Epub 2014 Nov 21.
  PUBMED    25416133
REFERENCE   2  (bases 1 to 8920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1161/CIRCRESAHA.116.305547"; journalName: "Circ Res"; date: 
            "2015-01-16- 16"; volume: "116"; issue: "2"; pages: "237-44"
COMMENT     Sequence Label: pMx-MGT
FEATURES             Location/Qualifiers
     source          1..8920
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             22..615
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    678..1035
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1094..1510
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1440..1462
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1478..1494
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1520..1894
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1832..1848
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             3237..3293
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4638..4691
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     LTR             6425..6894
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(7024..7041)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7195..7783)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7275..7294)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7947..8738,8739..8807))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8570..8589
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8808..8912)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta
       61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc
      121 aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
      181 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat
      241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
      301 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
      361 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
      421 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc
      481 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc
      541 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag
      601 cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg
      661 acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg
      721 tctatgactg attttatgcg cctgcgtcgg tactagttag ctaactagct ctgtatctgg
      781 cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg gagacgtccc
      841 agggacttcg ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg
      901 actctttggt gcacccccct aataggaggg atatgtggtt ctggtaggag acgagaacct
      961 aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc
     1021 gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt
     1081 gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa
     1141 agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac
     1201 cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa
     1261 ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc
     1321 agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt
     1381 caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc
     1441 ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc
     1501 tctaggcgcc cccatatggc catatgagat cttatatggg gcacccccgc cccttgtaaa
     1561 cttccctgac cctgacatga caagagttac taacagcccc tctctccaag ctcacttaca
     1621 ggctctctac ttagtccagc acgaagtctg gagacctctg gcggcagcct accaagaaca
     1681 actggaccga ccggtggtac ctcaccctta ccgagtcggc gacacagtgt gggtccgccg
     1741 acaccagact aagaacctag aacctcgctg gaaaggacct tacacagtcc tgctgaccac
     1801 ccccaccgcc ctcaaagtag acggcatcgc agcttggata cacgccgccc acgtgaaggc
     1861 tgccgacccc gggggtggac catcctctag actgccggat ctagctagtt aattaaggat
     1921 ccgccatggg gagaaaaaag attcagatta cgaggataat ggatgagcgt aacagacagg
     1981 tgacttttac gaagaggaaa tttggattga tgaagaaggc ttatgagctg agcgtgctgt
     2041 gcgactgtga gattgcactg atcatcttca acagcaccaa caagctgttc cagtacgcca
     2101 gcactgacat ggataaggtg ttgctcaagt acaccgagta caacgagccg cacgagagcc
     2161 ggacaaactc agacattgtg gaggcattga acaagaaaga aaacaaaggc tctgaaagcc
     2221 ccgatcctga ctcctcttat gcactcaccc cacgcactga agaaaaatac aaaaaaatta
     2281 atgaagaatt tgataatatg atcaagagtc ataaaattcc tgctgttcca cctcccagct
     2341 ttgagatgcc agttaccatc ccagtgtcca gccataacag tttggtgtac agcaatcctg
     2401 tcagcacact gggaaacccc aatcttctgc cactggccca cccgtctctg cagaggaata
     2461 gtatgtctcc tggtgtaaca catagacctc caagtgcagg taacacaggc ggtctgatgg
     2521 gcggagatct gacatccggt gcaggcacca gcgcagggaa tggatacggc aacccccgga
     2581 actcaccagg cctgctggtc tcacctggta acctgaacaa gaatatacaa gccaaatctc
     2641 ctccccctat gaatctagga atgaataatc gtaagccaga tctccgcgtt cttatcccac
     2701 ctggcagcaa gaacacgatg ccatcagtga atcaaaggat aaataactcc cagtcggctc
     2761 agtcattggc taccccggtg gtttccgtag caactcctac tttaccagga caaggaatgg
     2821 gaggatatcc atcagccatt tcaacaacat atggtactga gtactctctg agtagcgcag
     2881 atctgtcatc tctgtctggc ttcaacactg ccagtgcgct ccacctcggc tctgtaactg
     2941 gctggcagca gcagcaccta cataacatgc cgccatctgc cctcagtcag ttgggagacc
     3001 gtaccaccac cccttcgaga tacccacaac acaccacgcg ccacgaggcg gggaggtctc
     3061 ctgttgacag cttgagcagc tgtagcagtt cctacgatgg gagcgaccga gaggatcacc
     3121 ggaacgaatt ccactccccc attggactca ccagaccttc gccggacgaa agggaaagtc
     3181 cttcagtcaa gcgcatgcga ctctctgaag gatgggcaac agctagcggc agcggcgcca
     3241 cgaacttctc tctgttaaag caagcaggag atgttgaaga aaaccccggg cctgcatgca
     3301 tgtaccaaag cctggccatg gccgccaacc acggcccccc gcccggcgcc tacgaagcag
     3361 gtggccctgg cgccttcatg cacagcgcgg gcgccgcgtc ctcgcccgtc tacgtgccca
     3421 ctccgcgggt gccgtcctct gtgctgggcc tgtcctacct gcagggcggt ggcagtgccg
     3481 ctgcagctgg aaccacctcg ggtggcagct ccggggccgg cccgtcgggt gcagggcctg
     3541 ggacccagca gggtagccct ggctggagcc aagctggagc cgagggagcc gcctacaccc
     3601 cgccgcccgt gtccccgcgc ttctctttcc cggggactac tgggtccctg gcggccgctg
     3661 ccgccgctgc cgcagcccgg gaagctgcag cctacggcag tggcggcggg gcggcgggcg
     3721 ctggtctggc tggccgagag cagtacgggc gtccgggctt cgccggctcc tactccagcc
     3781 cctacccagc ctacatggcc gacgtgggag catcctgggc cgcagccgct gccgcctctg
     3841 ccggcccctt cgacagccca gtcctgcaca gcctgcctgg acgggccaac cctggaagac
     3901 accccaatct cgatatgttt gatgacttct cagaaggcag agagtgtgtc aattgtgggg
     3961 ccatgtccac cccactctgg aggcgagatg ggacgggaca ctacctgtgc aatgcctgtg
     4021 gcctctatca caagatgaac ggcatcaacc ggcccctcat taagcctcag cgccgcctgt
     4081 ccgcttcccg ccgggtaggc ctctcctgtg ccaactgcca gactaccacc accacgctgt
     4141 ggcgtcgtaa tgccgagggt gagcctgtat gtaatgcctg cggcctctac atgaagctcc
     4201 atggggttcc caggcctctt gcaatgcgga aggaggggat tcaaaccaga aaacggaagc
     4261 ccaagaacct gaataaatct aagacgccag caggtcctgc tggtgagacc ctccctccct
     4321 ccagtggtgc ctccagcggt aactccagca atgccactag cagcagcagc agcagtgaag
     4381 agatgcgccc catcaagaca gagcccgggc tgtcatctca ctatgggcac agcagctcca
     4441 tgtcccagac attcagtact gtgtccggcc acgggccctc catccatcca gtgctgtctg
     4501 ctctgaagct gtccccacaa ggctatgcat ctcctgtcac tcagacatcg caggccagct
     4561 ccaagcagga ctcttggaac agcctggtcc tggctgacag tcatggggac ataatcaccg
     4621 cggcatgcgg ctccggcgag ggcaggggaa gtcttctaac atgcggggac gtggaggaaa
     4681 atcccggccc actcgagatg gccgatacag atgagggctt tggcctggcg cgcacgcctc
     4741 tggagcctga ttccaaagac aggtcttgcg attcgaaacc tgagagtgct ctgggggctc
     4801 ccagcaagtc tccatcatcc ccgcaggctg ccttcaccca gcagggcatg gaaggaatca
     4861 aggtgtttct tcatgaacgt gaactgtggc tgaagttcca cgaagtgggc acagagatga
     4921 tcatcaccaa ggcagggagg agaatgtttc ctagttacaa agtgaaggtg actggcctta
     4981 atcccaaaac gaagtatatt cttctcatgg atattgttcc cgcagacgac cacagatata
     5041 aatttgctga taacaaatgg tccgtaactg gcaaagcaga gcctgccatg ccggggcgcc
     5101 tttacgtgca cccggactcc ccagcaaccg gagcccactg gatgcgacaa cttgtctcct
     5161 tccagaagct caaactcacc aacaaccacc tggacccgtt tggacacatt atcctgaact
     5221 ccatgcacaa ataccagccc cgattacaca tcgtgaaagc agacgaaaat aatgggttcg
     5281 gttcaaagaa cactgcgttt tgcacccacg tcttcccgga gacagctttt atcgctgtga
     5341 cttcgtacca gaatcacaag atcacacagc tgaaaattga gaacaacccc ttcgccaaag
     5401 gctttcgggg cagtgatgac ctggagttac acaggatgtc tcggatgcaa agtaaagagt
     5461 atcctgtggt tcccaggagc acagtgaggc acaaagtcac ctccaaccac agccccttca
     5521 gcagcgagac ccgagctctc tccacctcat ccaatttagg gtcccagtac cagtgtgaga
     5581 atggtgtctc tggcccctcc caggaccttc tgcccccacc taacccatac ccactggccc
     5641 aggagcacag ccaaatttac cactgtacca agaggaaaga tgaggaatgt tccagcacgg
     5701 agcaccccta taagaagccg tacatggaga catcccccag cgaggaagac accttctatc
     5761 gctcgggcta cccccagcag cagggcctga gtacctctta caggacagag tcggcccagc
     5821 ggcaggcctg catgtatgcc agctccgctc cccccagcga gcccgtgcct agcctggagg
     5881 acatcagctg taacacatgg cccagcatgc cctcctatag cagctgtacc gtcaccaccg
     5941 tgcagcccat ggaccgtctt ccctaccagc acttctccgc tcatttcacc tcggggcccc
     6001 tggtccctcg gttggctggc atggccaacc atggttctcc ccagctcggc gaagggatgt
     6061 ttcagcacca gacctcagtg gcccatcagc ctgtggtcag gcagtgcggg cctcagactg
     6121 gccttcagtc tccgggcggc ctccagcccc cagagtttct ctacactcac ggcgtgccca
     6181 ggaccctgtc cccccatcag tatcactcgg tacacggcgt cggcatggtg ccagagtgga
     6241 gtgagaatag ctaagtcgac gataaaataa aagattttat ttagtctcca gaaaaagggg
     6301 ggaatgaaag accccacctg taggtttggc aagctagctt aagtaacgcc attttgcaag
     6361 gcatggaaaa atacataact gagaatagag aagttcagat caaggtcagg aacagatgga
     6421 acagctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg
     6481 ccaagaacag atggaacagc tgaatatggg ccaaacagga tatctgtggt aagcagttcc
     6541 tgccccggct cagggccaag aacagatggt ccccagatgc ggtccagccc tcagcagttt
     6601 ctagagaacc atcagatgtt tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta
     6661 tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct
     6721 caataaaaga gcccacaacc cctcactcgg ggcgccagtc ctccgattga ctgagtcgcc
     6781 cgggtacccg tgtatccaat aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt
     6841 tccttgggag ggtctcctct gagtgattga ctacccgtca gcgggggtct ttcacatgca
     6901 gcatgtatca aaattaattt ggtttttttt cttaagtatt tacattaaat ggccatagtt
     6961 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     7021 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     7081 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     7141 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     7201 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     7261 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     7321 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     7381 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     7441 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     7501 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     7561 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     7621 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     7681 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     7741 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     7801 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     7861 attatcaaaa aggatcttca cctagatcct tttgcggccg gccgcaaatc aatctaaagt
     7921 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     7981 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     8041 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     8101 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     8161 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     8221 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     8281 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     8341 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     8401 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     8461 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     8521 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     8581 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     8641 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     8701 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     8761 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     8821 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     8881 atttagaaaa ataaacaaat aggggttccg cgcacatttc
//
