LOCUS       sequence-215740-        9061 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for inducible expression of N-terminally tagged 
            eGFP-SHLD1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9061)
  AUTHORS   Noordermeer SM, Adam S, Setiaputra D, Barazas M, Pettitt SJ, Ling 
            AK, Olivieri M, Alvarez-Quilon A, Moatti N, Zimmermann M, Annunziato
            S, Krastev DB, Song F, Brandsma I, Frankum J, Brough R, Sherker A, 
            Landry S, Szilard RK, Munro MM, McEwan A, Goullet de Rugy T, Lin ZY,
            Hart T, Moffat J, Gingras AC, Martin A, van Attikum H, Jonkers J, 
            Lord CJ, Rottenberg S, Durocher D
  TITLE     The shieldin complex mediates 53BP1-dependent DNA repair.
  JOURNAL   Nature. 2018 Aug;560(7716):117-121. doi: 10.1038/s41586-018-0340-7. 
            Epub 2018 Jul 18.
  PUBMED    30022168
REFERENCE   2  (bases 1 to 9061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-018-0340-7"; journalName: "Nature"; date: "2018-08";
            volume: "560"; issue: "7716"; pages: "117-121"
COMMENT     Sequence Label: pCW-eGFP-SHLD1
FEATURES             Location/Qualifiers
     source          1..9061
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    43..160
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        214..528
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    222..240
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    258..276
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    293..311
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    329..347
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    365..383
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    400..418
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    436..454
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     501..525
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    549..573
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(591..593,594..596,597..1307)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(591..593,594..596,597..1307)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(636..657)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(897..916)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1244..1265
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(1936..1960)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1978..1995
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        2005..2515
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2434..2452
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2525..3121
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2525..2544)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3021..3041
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             3122..3175
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(3176..3793,3794..3799,3800..3835,3836..3838,3839..3874,
                     3875..3877,3878..3913,3914..3922)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(3962..3982)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4380..4391)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4596..4829
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4901..5022
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4938..4957)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4992..5011
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5062..5197
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5197..5268
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5269..5337,5338..6129)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5487..5506)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6300..6888
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6789..6808
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7042..7059
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7176..7197
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7212..7217,7218..7235,7236..7242)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7250..7266
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7255..7277
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7274..7290
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7274..7290
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7309..7329
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7311..7329
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7357..7583
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7584..7764
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7811..7936
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8429..8662
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8847..8891
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 acaattctcg acctcgagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
       61 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
      121 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
      181 cagagatcca ctttggccgc gaatcgatat gtcgagttta ctccctatca gtgatagaga
      241 acgtatgtcg agtttactcc ctatcagtga tagagaacga tgtcgagttt actccctatc
      301 agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg tatgtcgagt
      361 ttactcccta tcagtgatag agaacgtatg tcgagtttat ccctatcagt gatagagaac
      421 gtatgtcgag tttactccct atcagtgata gagaacgtat gtcgaggtag gcgtgtacgg
      481 tgggaggcct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagaattggc
      541 tagcatcaac aagtttgtac aaaaaagcag gcttcgaagg agatagaacc atggtgagca
      601 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
      661 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
      721 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
      781 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
      841 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
      901 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
      961 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     1021 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     1081 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     1141 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     1201 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     1261 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagggc gcgccaatgg
     1321 cagccaggga cgccacttca ggcagcctgt cagaggagag cagtgctttg gacctgccat
     1381 cagcgtgtga cataagagat tacgtcctgc agggacccag ccaagaagcc aacagcgagg
     1441 ctttcagttc tttggaattc cattcttttc cttattcttc tgatgtggat ccagacacca
     1501 gtaacttaaa tatagaacaa aataactcct ggaccgctga gaacttctgg cttgaccctg
     1561 ctgtgaaagg ccagtcagag aaggaagagg atgatggcct tcggaaatcc ctggatagat
     1621 tctatgaaat gtttggtcat ccacagccag gctctgcaaa ctcactctct gcatctgtct
     1681 gcaagtgcct gtctcagaaa atcactcaac taagaggcca ggagagccaa aagtatgccc
     1741 tccgcagttt tcaaatggcc cgggtgatct tcaaccggga cggctgctcc gtcttacaga
     1801 ggcattccag ggacacccac ttctacccac tggaggaagg aagtacatct ttggatgatg
     1861 aaaagccaaa cccaggactg tcaaaggata ttactcattt cctcttgcag cagaatgtaa
     1921 tgaaagacct gtaggaccca gctttcttgt acaaagtggt ttagtaatga accggtccac
     1981 caccaccacc accactaagg atccggggtt ggggttgcgc cttttccaag gcagccctgg
     2041 gtttgcgcag ggacgcggct gctctgggcg tggttccggg aaacgcagcg gcgccgaccc
     2101 tgggtctcgc acattcttca cgtccgttcg cagcgtcacc cggatcttcg ccgctaccct
     2161 tgtgggcccc ccggcgacgc ttcctgctcc gcccctaagt cgggaaggtt ccttgcggtt
     2221 cgcggcgtgc cggacgtgac aaacggaagc cgcacgtctc actagtaccc tcgcagacgg
     2281 acagcgccag ggagcaatgg cagcgcgccg accgcgatgg gctgtggcca atagcggctg
     2341 ctcagcaggg cgcgccgaga gcagcggccg ggaaggggcg gtgcgggagg cggggtgtgg
     2401 ggcggtagtg tgggccctgt tcctgcccgc gcggtgttcc gcattctgca agcctccgga
     2461 gcgcacgtcg gcagtcggct ccctcgttga ccgaatcacc gacctctctc cccagcaatt
     2521 caccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc
     2581 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc
     2641 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     2701 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     2761 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     2821 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     2881 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     2941 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     3001 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     3061 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     3121 cgaaggtaga ggttctctcc tcacttgtgg tgatgttgaa gaaaaccctg gtccaatgtc
     3181 tagactggac aagagcaaag tcataaacgg agctctggaa ttactcaatg gtgtcggtat
     3241 cgaaggcctg acgacaagga aactcgctca aaagctggga gttgagcagc ctaccctgta
     3301 ctggcacgtg aagaacaagc gggccctgct cgatgccctg ccaatcgaga tgctggacag
     3361 gcatcatacc cacttctgcc ccctggaagg cgagtcatgg caagactttc tgcggaacaa
     3421 cgccaagtca taccgctgtg ctctcctctc acatcgcgac ggggctaaag tgcatctcgg
     3481 cacccgccca acagagaaac agtacgaaac cctggaaaat cagctcgcgt tcctgtgtca
     3541 gcaaggcttc tccctggaga acgcactgta cgctctgtcc gccgtgggcc actttacact
     3601 gggctgcgta ttggaggaac aggagcatca agtagcaaaa gaggaaagag agacacctac
     3661 caccgattct atgcccccac ttctgagaca agcaattgag ctgttcgacc ggcagggagc
     3721 cgaacctgcc ttccttttcg gcctggaact aatcatatgt ggcctggaga aacagctaaa
     3781 gtgcgaaagc ggcgggccga ccgacgccct tgacgatttt gacttagaca tgctcccagc
     3841 cgatgccctt gacgactttg accttgatat gctgcctgct gacgctcttg acgattttga
     3901 ccttgacatg ctccccgggt aactaagtaa ggatcgatcc aagatatcgt attcttaact
     3961 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     4021 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     4081 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     4141 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     4201 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     4261 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat
     4321 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     4381 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     4441 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg
     4501 tttcgcctcg gcgtccggac tagaggtacc tttaagacca atgacttaca aggcagctgt
     4561 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     4621 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     4681 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     4741 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     4801 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
     4861 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
     4921 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     4981 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
     5041 atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     5101 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     5161 ctattccaga agtagtgagg aggctttttt ggaggccttt caaatatgta tccgctcatg
     5221 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     5281 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     5341 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     5401 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     5461 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     5521 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     5581 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     5641 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     5701 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     5761 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     5821 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     5881 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     5941 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     6001 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     6061 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     6121 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     6181 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     6241 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     6301 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     6361 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     6421 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     6481 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     6541 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     6601 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     6661 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     6721 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     6781 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     6841 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     6901 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     6961 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     7021 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     7081 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     7141 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     7201 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     7261 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct
     7321 cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt
     7381 agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc
     7441 gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga
     7501 cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta
     7561 agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc
     7621 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     7681 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     7741 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
     7801 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
     7861 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
     7921 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     7981 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     8041 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     8101 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     8161 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     8221 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     8281 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     8341 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     8401 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     8461 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     8521 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     8581 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     8641 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     8701 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     8761 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     8821 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     8881 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     8941 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     9001 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     9061 g
//
