LOCUS       sequence-215742-        3775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Conditional ablation of mature bone cells via ganciclovir exposure 
            of cells expressing a truncated thymidine kinase..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Visnjic D, Kalajzic I, Gronowicz G, Aguila HL, Clark SH, Lichtler 
            AC, Rowe DW
  TITLE     Conditional ablation of the osteoblast lineage in Col2.3deltatk 
            transgenic mice.
  JOURNAL   J Bone Miner Res. 2001 Dec;16(12):2222-31. doi: 
            10.1359/jbmr.2001.16.12.2222.
  PUBMED    11760835
REFERENCE   2  (bases 1 to 3775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1359/jbmr.2001.16.12"; journalName: "J Bone Miner Res"; date: 
            "2001-12"; volume: "16"; issue: "12"; pages: "2222-31"
COMMENT     Sequence Label: Col2.3deltaTK
FEATURES             Location/Qualifiers
     source          1..3775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2440..3435
                     /codon_start=1
                     /product="truncated herpes simplex virus thymidine kinase 
                     (Salomon et al., 1995)"
                     /label=Delta-TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWQV
                     LGASETIANIYTTQHRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGS
                     SHAPPPALTLIFDRHPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGAL
                     PEDRHIDRLAKRQRPGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAV
                     PPQGAEPQSNAGPRPHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFI
                     LDYDQSPAGCRDALLQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     primer_bind     complement(3433..3452)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3533..3757
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 atcgataagc ttggaaccca ctgcccacaa ctcctggggc aaaaacctgg agtcagacat
       61 ggggtgaagg ctgtcacacg gcacagacac gtcaagcacc cccccccaat tctagtagtc
      121 tcctagcctc caccagaacc ccagaccctt gatgtggcag tcaccagtcc acacctgtta
      181 ggctcttgtc tcttcttcca gatgagcctg gggggcgggg ggggtgctag atcaggagca
      241 gggaaaagta gctttggata agtgcttttc ccaatacaaa acccaacaaa gagtgggcag
      301 atcacactgt gtagtgcttc gtggaaccct accctagaca actgccttga acacctattc
      361 cctctgatgt acaccatccc cgtccactgt tagggagtgg gcatcctttg gaactgacca
      421 ctgtggaagg caggacttta ctgagttccg gaactaccat ctcagcttct cagccccagc
      481 cttaccctac aggcactggc ataggcgggg gcagatcctg ggccacaagt cactgccaca
      541 tggttgggat aattgatgaa gtcctgtcct tccattgctg tctccagttc tgcttctctg
      601 gaaactctat atttttccct ttaattatag cctctgcagt ctccctctgc caccccaccc
      661 gcaccgctta gcctaactgc ccacggccag cgacgtggct ccctcccctt ctgctccctt
      721 ggtctttttt attttttttt ctttgccttc gttgcacaaa actagctcag ggagggcgtg
      781 aagggggggg gagcaatgga atcttggatg gtttggagga ggcgggactc cttgcttcca
      841 cgtttacagc tctgaagacg gctgtggggg aagtgataca ggacgtctat gggccctgag
      901 aggagacccc tatgcttccc tgccacccac acagtttaac aaaatgaagt tcctaagtag
      961 agtgggggtc aggcagagca cctttgcagg gttgatggga gcccagggaa agaaaggaca
     1021 ctgtctttta gggacacatt taaatataag ccacttttct tgggggacga caaatgaccc
     1081 tttcctgatt gcagaggtgg ggaacaatgg ctgagatttt cagcaaagaa gcgaggacat
     1141 gaggagtagc cttcaaataa agtcactcag ctaccaaaaa caagtttctg ccacacaccg
     1201 agttcaccta ggtgtcccca gaccagatcc aagtacagta aggaaagcag gttctctaca
     1261 gagagaacac ggctctatgg ccaatgcctt ctacctgctc tttctggatt gatactgcta
     1321 cctaagaggg cctctaacca attcctggct gtagccacag ctgacacaag acctttttct
     1381 aagacatccc tggtcacagg cctcctgtag caaattccag ccctgggatg gaggtggtca
     1441 ggaaagagtt tatacaagaa gacccaggcc acagctttaa ggactcagaa acccccctgc
     1501 ccacacggct gcccatcata acgcagaagg tttcttctgg aaggacaagg atgtcaaact
     1561 tctccccaag cctaatcctc agagatgtct ccctctgtta cacctggggc tggagaaagg
     1621 tgggtctttc atggagccac attcatggca gaacagatag ccaccccact cctttcaaac
     1681 aaccacatat ctgactctta gtatctgtga agagatgtct aatttgttcc caaatagtcc
     1741 taccctgcat acctgggccc acaccatgag gtattctcct ccctctaaca gtcacatctg
     1801 cttagctgcc tggttcttcg gatttggaga gatgcttgcc taacttattc ttccttaggt
     1861 cttcccaagg atgccagaaa gactatgaga catggccaag aggacctttt cccaattgtg
     1921 cctgacactg aaccctttgt aatgttcccc aactcagatt cccaattcta catccttctg
     1981 atttgaggtc ccagaaggaa agtgcaaggg gcatccccta cccacaatca gtatatcgag
     2041 ggcccagcca cactcagtga tagcacctct ggcccatgta gatctggggg acaagggtgg
     2101 cagaattgca aaggggggag ggggctgggt ggactccttt cccttccttt ccctcctccc
     2161 ccctcttcgt tccaaattgg gggccgggcc aggcagttct gattggctgg gggccgggct
     2221 gctggctccc cctctccaag aggcagggtt cctcccagcc cgccctcctc catcaggatg
     2281 gtataaaagg ggcccaggcc agtcgtcgga gcagacggga gtttcacctc cggacggagc
     2341 aggaggcaca cggagtgagg ccacgcatga gccgaagcta accccccacc ccagccgcaa
     2401 agagtctaca tgtctagggt ctagaggatc cgcgagctta tgcccacgct actgcgcgtt
     2461 tatatagacg gtcctcacgg gatggggaaa accaccacca cgcaactgct ggtggccctg
     2521 ggttcgcgcg acgatatcgt ctacgtaccc gagccgatga cttactggca ggtgctgggg
     2581 gcttccgaga caatcgcgaa catctacacc acacaacacc gcctcgacca gggtgagata
     2641 tcggccgggg acgcggcggt ggtaatgaca agcgcccaga taacaatggg catgccttat
     2701 gccgtgaccg acgccgttct ggctcctcat atcggggggg aggctgggag ctcacatgcc
     2761 ccgcccccgg ccctcaccct catcttcgac cgccatccca tcgccgccct cctgtgctac
     2821 ccggccgcgc gataccttat gggcagcatg accccccagg ccgtgctggc gttcgtggcc
     2881 ctcatcccgc cgaccttgcc cggcacaaac atcgtgttgg gggcccttcc ggaggacaga
     2941 cacatcgacc gcctggccaa acgccagcgc cccggcgagc ggcttgacct ggctatgctg
     3001 gccgcgattc gccgcgttta cgggctgctt gccaatacgg tgcggtatct gcagggcggc
     3061 gggtcgtggc gggaggattg gggacagctt tcggggacgg ccgtgccgcc ccagggtgcc
     3121 gagccccaga gcaacgcggg cccacgaccc catatcgggg acacgttatt taccctgttt
     3181 cgggcccccg agttgctggc ccccaacggc gacctgtaca acgtgtttgc ctgggccttg
     3241 gacgtcttgg ccaaacgcct ccgtcccatg cacgtcttta tcctggatta cgaccaatcg
     3301 cccgccggct gccgggacgc cctgctgcaa cttacctccg ggatggtcca gacccacgtc
     3361 accacccccg gctccatacc gacgatctgc gacctggcgc gcacgtttgc ccgggagatg
     3421 ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg ctatgacggc
     3481 aataaaagga cagaataaaa cgcacgggtg gatccccggg cgagctcgaa ttctgtgcct
     3541 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     3601 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     3661 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     3721 aatagcaggc atgctgggga tgcggtgggc tctatggaat tccgagctta tcgat
//
