LOCUS       sequence-21575-e         682 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Kaykas A, Moon RT
  TITLE     A plasmid-based system for expressing small interfering RNA 
            libraries in mammalian cells.
  JOURNAL   BMC Cell Biol. 2004 Apr 30;5:16. doi: 10.1186/1471-2121-5-16.
  PUBMED    15119963
REFERENCE   2  (bases 1 to 682)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Cell 
            Biol."; date: "2004-04-30"; volume: "5:16. doi"; pages: " 
            10.1186/1471-2121-5-16"
COMMENT     Sequence Label: V44 pHIPPY blunt
FEATURES             Location/Qualifiers
     source          1..682
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(58..298)
                     /label=U6 promoter
                     /note="Truncated RNA polymerase III promoter for human U6 
                     snRNA used in pSIREN vectors such as 
                     pSIREN-RetroQ-ZsGreen1. Expected transcription start site 
                     is 8bp downstream of this feature."
     primer_bind     complement(108..127)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(278..298)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 aggagcttgg attgggatct tatagtggat cctgagacca ctctgaaaat tttcttcgtg
       61 ctagccacaa gatatataaa gccaagaaat cgaaatactt tcaagttacg gtaagcatat
      121 gatagtccat tttaaaacat aattttaaaa ctgcaaacta cccaagaaat tattactttc
      181 tacgtcacgt attttgtact aatatctttg tgtttacagt caaattaatt ctaattatct
      241 ctctaacagc cttgtatcgt atatgcaaat atgaaggaat catgggaaat aggccctctt
      301 cctgcccgac cttggcgcgc gctcggcgcg cggtcacgct ccgtcacgtg gtgcgttttg
      361 cctgcccgtc tttccactgg ggacgcgtgg tgtggaaagt ccccaggctc cccagcaggc
      421 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
      481 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
      541 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
      601 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
      661 cagaagtagt gaggaggctt tg
//
