LOCUS       sequence-215752-        5096 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ProA driven gfpmut3 expression with synTT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5096)
  AUTHORS   Cooper KG, Chong A, Starr T, Finn CE, Steele-Mortimer O
  TITLE     Predictable, Tunable Protein Production in Salmonella for Studying 
            Host-Pathogen Interactions.
  JOURNAL   Front Cell Infect Microbiol. 2017 Nov 16;7:475. doi: 
            10.3389/fcimb.2017.00475. eCollection 2017.
  PUBMED    29201859
REFERENCE   2  (bases 1 to 5096)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Cell 
            Infect Microbiol."; date: "2017-11-16"; volume: "7:475. doi"; pages:
            " 10.3389/fcimb.2017.00475. eCollection 2017"
COMMENT     Sequence Label: pCON1-ProA.gfp
FEATURES             Location/Qualifiers
     source          1..5096
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     13..31
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             265..978
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(295..323)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     918..938
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      1018..1089
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1105..1132
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     oriT            1746..1833
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     primer_bind     complement(3146..3163)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3317..3905)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3397..3416)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4076..4867,4868..4936))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4699..4718
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4937..5041)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcaagaatt cgagctcggt
       61 acccgggcac agctaacacc acgtcgtccc tatctgctgc cctaggtcta tgagtggttg
      121 ctggataact ttacgggcat gcataaggct cgtaggctat attcagggag accacaacgg
      181 tttccctcta caaataattt tgtttaactt ttactagagt cacacaggaa agtactagtc
      241 tagatttaag aaggagatat acatatgagt aaaggagaag aacttttcac tggagttgtc
      301 ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag
      361 ggtgaaggtg atgcaacata cggaaaactt acccttaaat ttatttgcac tactggaaaa
      421 ctacctgttc catggccaac acttgtcact actttcggtt atggtgttca atgctttgcg
      481 agatacccag atcatatgaa acagcatgac tttttcaaga gtgccatgcc cgaaggttat
      541 gtacaggaaa gaactatatt tttcaaagat gacgggaact acaagacacg tgctgaagtc
      601 aagtttgaag gtgataccct tgttaataga atcgagttaa aaggtattga ttttaaagaa
      661 gatggaaaca ttcttggaca caaattggaa tacaactata actcacacaa tgtatacatc
      721 atggcagaca aacaaaagaa tggaatcaaa gttaacttca aaattagaca caacattgaa
      781 gatggaagcg ttcaactagc agaccattat caacaaaata ctccaattgg cgatggccct
      841 gtccttttac cagacaacca ttacctgtcc acacaatctg ccctttcgaa agatcccaac
      901 gaaaagagag accacatggt ccttcttgag tttgtaacag ctgctgggat tacacatggc
      961 atggatgaac tatacaaata aatgtccaga cctgcaggca tgcaagcttc caggcatcaa
     1021 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     1081 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttataaa
     1141 gctttaatgc ggtagtttat cacagttaaa ttgctaacgc agtcaggcac cgtgtatgaa
     1201 atctaacaat gcgctcatcg tcatcctcgg caccgtcacc ctggatgctg taggcatagg
     1261 cttggttatg ccggtactgc cgggcctctt gcgggatatc gtccattccg acagcatcgc
     1321 cagtcactat ggcgtgctgc tagcgctttt agccgcttta gcggcctttc cccctacccg
     1381 aagggtgggg gcgcgtgtgc agccccgcag ggcctgtctc ggtcgatcat tcagcccggc
     1441 tcatccttct ggcgtggcgg cagaccgaac aaggcgcggt cgtggtcgcg ttcaaggtac
     1501 gcatccattg ccgccatgag ccgatcctcc ggccactcgc tgctgttcac cttggccaaa
     1561 atcatggccc ccaccagcac cttgcgcctt gtttcgttct tgcgctcttg ctgctgttcc
     1621 cttgcccgca cccgctgaat ttcggcattg attcgcgctc gttgttcttc gagcttggcc
     1681 agccgatccg ccgccttgtt gctcccctta accatcttga caccccattg ttaatgtgct
     1741 gtctcgtagg ctatcatgga ggcacagcgg cggcaatccc gaccctactt tgtaggggag
     1801 ggcgcactta ccggtttctc ttcgagaaac tggcctaacg gccacccttc gggcggtgcg
     1861 ctctccgagg gccattgcat ggagccgaaa agcaaaagca acagcgaggc agcatggcga
     1921 tttatcacct tacggcgaaa accggcagca ggtcgggcgg ccaatcggcc agggccaagg
     1981 ccgactacat ccagcgcgaa ggcaagtatg cccgcgacat ggatgaagtc ttgcacgccg
     2041 aatccgggca catgccggag ttcgtcgagc ggcccgccga ctactgggat gctgccgacc
     2101 tgtatgaacg cgccaatggg cggctgttca aggaggtcga atttgccctg ccggtcgagc
     2161 tgaccctcga ccagcagaag gcgctggcgt ccgagttcgc ccagcacctg accggtgccg
     2221 agcgcctgcc gtatacgctg gccatccatg ccggtggcgg cgagaacccg cactgccacc
     2281 tgatgatctc cgagcggatc aatgacggca tcgagcggcc cgccgctcag tggttcaagc
     2341 ggtacaacgg caagaccccg gagaagggcg gggcacagaa gaccgaagcg ctcaagccca
     2401 aggcatggct tgagcagacc cgcgaggcat gggccgacca tgccaaccgg gcattagagc
     2461 gggctggcca cgacgcccgc attgaccaca gaacacttga ggcgcagggc atcgagcgcc
     2521 tgcccggtgt tcacctgggg ccgaacgtgg tggagatgga aggccggggc atccgcaccg
     2581 accgggcaga cgtggccctg aacatcgaca ccgccaacgc ccagatcatc gacttacagg
     2641 aataccggga ggcaatagac catgaacgca atcgacagag tgaagaaatc cagaggcatc
     2701 aacgagttag cggagcagat cgaaccgctg gcccagagca tggcgacact ggccgacgaa
     2761 gcccggcagg tcatgagcca gacccagcag gccagcgagg cgcaggcggc ggagtggctg
     2821 aaagcccagc gccagacagg ggcggcatgg gtggagctgg ccaaagagtt gcgggaggta
     2881 gccgccgagg tgagcagcgc cgcgcagagc gcccggagcg cgtcgcgggg gtggcactgg
     2941 aagctatggc taaccgtgat gctggcttcc atgatgccta cggtggtgct gctgatcgca
     3001 tcgttgctct tgctcgacct gacgccactg acaaccgagg acggctcgat ctggctgcgc
     3061 ttggtggccc gatgaagaac gacaggactt tgcaggccat aggccgacag ctcaaggcca
     3121 tgggctgtga gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     3181 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     3241 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     3301 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     3361 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     3421 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     3481 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     3541 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     3601 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     3661 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     3721 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     3781 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     3841 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     3901 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     3961 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     4021 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     4081 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     4141 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     4201 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     4261 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     4321 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     4381 ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     4441 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     4501 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     4561 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     4621 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     4681 gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     4741 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     4801 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     4861 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     4921 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     4981 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     5041 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgaca
//
