LOCUS       sequence-215754-        5074 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ProB driven mCherry expression with synTT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5074)
  AUTHORS   Cooper KG, Chong A, Starr T, Finn CE, Steele-Mortimer O
  TITLE     Predictable, Tunable Protein Production in Salmonella for Studying 
            Host-Pathogen Interactions.
  JOURNAL   Front Cell Infect Microbiol. 2017 Nov 16;7:475. doi: 
            10.3389/fcimb.2017.00475. eCollection 2017.
  PUBMED    29201859
REFERENCE   2  (bases 1 to 5074)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Cell 
            Infect Microbiol."; date: "2017-11-16"; volume: "7:475. doi"; pages:
            " 10.3389/fcimb.2017.00475. eCollection 2017"
COMMENT     Sequence Label: pCON1-ProB.mCherry
FEATURES             Location/Qualifiers
     source          1..5074
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             136..846
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(281..299)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     549..568
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      867..938
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      954..981
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     oriT            1595..1682
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     primer_bind     complement(2995..3012)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3166..3754)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3246..3265)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3925..4716,4717..4785))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4548..4567
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4786..4890)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4958..4976
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tttacgggca tgcataaggc tcgtaatata tattcaggga gaccacaacg gtttccctct
       61 acaaataatt ttgtttaact tttactagag tcacacagga aagtactagt ctagatttaa
      121 gaaggagata tacatatggt gagcaagggc gaggaggata acatggccat catcaaggag
      181 ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag
      241 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag
      301 ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag
      361 gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc
      421 ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac
      481 tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc
      541 tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg
      601 taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc
      661 ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc
      721 ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc
      781 gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac
      841 aagtaagcat gcaagcttcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
      901 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac
      961 cttcgggtgg gcctttctgc gtttataaag ctttaatgcg gtagtttatc acagttaaat
     1021 tgctaacgca gtcaggcacc gtgtatgaaa tctaacaatg cgctcatcgt catcctcggc
     1081 accgtcaccc tggatgctgt aggcataggc ttggttatgc cggtactgcc gggcctcttg
     1141 cgggatatcg tccattccga cagcatcgcc agtcactatg gcgtgctgct agcgctttta
     1201 gccgctttag cggcctttcc ccctacccga agggtggggg cgcgtgtgca gccccgcagg
     1261 gcctgtctcg gtcgatcatt cagcccggct catccttctg gcgtggcggc agaccgaaca
     1321 aggcgcggtc gtggtcgcgt tcaaggtacg catccattgc cgccatgagc cgatcctccg
     1381 gccactcgct gctgttcacc ttggccaaaa tcatggcccc caccagcacc ttgcgccttg
     1441 tttcgttctt gcgctcttgc tgctgttccc ttgcccgcac ccgctgaatt tcggcattga
     1501 ttcgcgctcg ttgttcttcg agcttggcca gccgatccgc cgccttgttg ctccccttaa
     1561 ccatcttgac accccattgt taatgtgctg tctcgtaggc tatcatggag gcacagcggc
     1621 ggcaatcccg accctacttt gtaggggagg gcgcacttac cggtttctct tcgagaaact
     1681 ggcctaacgg ccacccttcg ggcggtgcgc tctccgaggg ccattgcatg gagccgaaaa
     1741 gcaaaagcaa cagcgaggca gcatggcgat ttatcacctt acggcgaaaa ccggcagcag
     1801 gtcgggcggc caatcggcca gggccaaggc cgactacatc cagcgcgaag gcaagtatgc
     1861 ccgcgacatg gatgaagtct tgcacgccga atccgggcac atgccggagt tcgtcgagcg
     1921 gcccgccgac tactgggatg ctgccgacct gtatgaacgc gccaatgggc ggctgttcaa
     1981 ggaggtcgaa tttgccctgc cggtcgagct gaccctcgac cagcagaagg cgctggcgtc
     2041 cgagttcgcc cagcacctga ccggtgccga gcgcctgccg tatacgctgg ccatccatgc
     2101 cggtggcggc gagaacccgc actgccacct gatgatctcc gagcggatca atgacggcat
     2161 cgagcggccc gccgctcagt ggttcaagcg gtacaacggc aagaccccgg agaagggcgg
     2221 ggcacagaag accgaagcgc tcaagcccaa ggcatggctt gagcagaccc gcgaggcatg
     2281 ggccgaccat gccaaccggg cattagagcg ggctggccac gacgcccgca ttgaccacag
     2341 aacacttgag gcgcagggca tcgagcgcct gcccggtgtt cacctggggc cgaacgtggt
     2401 ggagatggaa ggccggggca tccgcaccga ccgggcagac gtggccctga acatcgacac
     2461 cgccaacgcc cagatcatcg acttacagga ataccgggag gcaatagacc atgaacgcaa
     2521 tcgacagagt gaagaaatcc agaggcatca acgagttagc ggagcagatc gaaccgctgg
     2581 cccagagcat ggcgacactg gccgacgaag cccggcaggt catgagccag acccagcagg
     2641 ccagcgaggc gcaggcggcg gagtggctga aagcccagcg ccagacaggg gcggcatggg
     2701 tggagctggc caaagagttg cgggaggtag ccgccgaggt gagcagcgcc gcgcagagcg
     2761 cccggagcgc gtcgcggggg tggcactgga agctatggct aaccgtgatg ctggcttcca
     2821 tgatgcctac ggtggtgctg ctgatcgcat cgttgctctt gctcgacctg acgccactga
     2881 caaccgagga cggctcgatc tggctgcgct tggtggcccg atgaagaacg acaggacttt
     2941 gcaggccata ggccgacagc tcaaggccat gggctgtgag cgctcttccg cttcctcgct
     3001 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     3061 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     3121 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     3181 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     3241 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     3301 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     3361 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     3421 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     3481 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     3541 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     3601 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     3661 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     3721 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     3781 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     3841 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     3901 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     3961 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     4021 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     4081 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     4141 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     4201 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg
     4261 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     4321 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     4381 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     4441 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     4501 atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc
     4561 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     4621 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     4681 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     4741 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     4801 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     4861 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     4921 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
     4981 tcgtcttcaa gaattcgagc tcggtacccg ggcacagcta acaccacgtc gtccctatct
     5041 gctgccctag gtctatgagt ggttgctgga taac
//
