LOCUS       sequence-215756-        2380 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BTK Type 3 coding sequence for golden gate assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Leonard SP, Perutka J, Powell JE, Geng P, Richhart DD, Byrom M, Kar 
            S, Davies BW, Ellington AD, Moran NA, Barrick JE
  TITLE     Genetic Engineering of Bee Gut Microbiome Bacteria with a Toolkit 
            for Modular Assembly of Broad-Host-Range Plasmids.
  JOURNAL   ACS Synth Biol. 2018 May 18;7(5):1279-1290. doi: 
            10.1021/acssynbio.7b00399. Epub 2018 Apr 13.
  PUBMED    29608282
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.7b00399"; journalName: "ACS Synth Biol"; date: 
            "2018-05-18- 18"; volume: "7"; issue: "5"; pages: "1279-1290"
COMMENT     Sequence Label: pBTK205
FEATURES             Location/Qualifiers
     source          1..2380
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      97..685
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     586..605
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1531..1565)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1636..2295)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2210..2229
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 cctcttacgt gcccgatcaa tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
       61 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
      121 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
      181 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
      241 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
      301 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
      361 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
      421 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
      481 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
      541 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
      601 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
      661 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
      721 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
      781 ccgtagtcgg tctcatatga gtaaaggaga agagcttttc acaggagttg tcccaatcct
      841 cgtggaatta gacggtgatg ttaatgggca caagttctct gtcagtggag agggtgaagg
      901 cgacgcaaca tatggcaagc tgacccttaa atttatttgc accacgggta aactacctgt
      961 tccatggcca acactggtca ctacgttcgg gtatggggtt cagtgctttg cgcgctaccc
     1021 agatcacatg aaacagcacg actttttcaa gagtgcaatg cccgaaggct atgtacagga
     1081 gagaaccatc ttttttaagg atgacggcaa ctataagaca cgcgccgaag tgaagttcga
     1141 gggtgatacc cttgttaata gaatcgagtt aaagggtatt gactttaagg aagatggaaa
     1201 tattttaggc cacaaactgg aatataacta taactcccat aatgtgtaca ttatggccga
     1261 caagcaaaag aacggtatca aggttaactt caagatcaga cacaacattg aggatggaag
     1321 cgttcaacta gccgaccatt accaacaaaa caccccaatt ggcgatgggc ctgtgctgtt
     1381 accagacaac cattacctgt ccactcaatc tgccctttcg aaagatccca acgaaaagcg
     1441 cgaccacatg gtccttcttg agtttgtcac ggctgctggg attacacacg gcatggatga
     1501 actatacaaa taaatcctga gaccagacca ataaaaaacg cccggcggca accgagcgtt
     1561 ctgaacaaat ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc
     1621 gagctcgata tcaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt
     1681 aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg
     1741 catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa
     1801 gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga
     1861 aacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca
     1921 cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca
     1981 gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc
     2041 ccatatcacc agctcaccgt ctttcattgc catacgaaat tccggatgag cattcatcag
     2101 gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt
     2161 taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg
     2221 aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt
     2281 gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac
     2341 gcccggtagt gatcttattt cattatggtg aaagttggaa
//
