LOCUS       sequence-21576-e         653 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Kaykas A, Moon RT
  TITLE     A plasmid-based system for expressing small interfering RNA 
            libraries in mammalian cells.
  JOURNAL   BMC Cell Biol. 2004 Apr 30;5:16. doi: 10.1186/1471-2121-5-16.
  PUBMED    15119963
REFERENCE   2  (bases 1 to 653)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Cell 
            Biol."; date: "2004-04-30"; volume: "5:16. doi"; pages: " 
            10.1186/1471-2121-5-16"
COMMENT     Sequence Label: V45 pHIPPY PGL3 Luciferase
FEATURES             Location/Qualifiers
     source          1..653
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(78..318)
                     /label=U6 promoter
                     /note="Truncated RNA polymerase III promoter for human U6 
                     snRNA used in pSIREN vectors such as 
                     pSIREN-RetroQ-ZsGreen1. Expected transcription start site 
                     is 8bp downstream of this feature."
     primer_bind     complement(128..147)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(298..318)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 agnagcttgg attgggatct tataagtgga tcctgagacc actctgaaaa ggctcctcag
       61 aaacagctct tttcttcgtg ctagccacaa gatatataaa gccaagaaat cgaaatactt
      121 tcaagttacg gtaagcatat gatagtccat tttaaaacat aattttaaaa ctgcaaacta
      181 cccaagaaat tattactttc tacgtcacgt attttgtact aatatctttg tgtttacagt
      241 caaattaatt ctaattatct ctctaacagc cttgtatcgt atatgcaaat atgaaggaat
      301 catgggaaat aggccctctt cctgcccgac cttggcgcgc gctcggcgcg cggtcacgct
      361 ccgtcacgtg gtgcgttttg cctgcccgtc tttccactgg ggacgcgtgg tgtggaaagt
      421 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
      481 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
      541 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
      601 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gcg
//
