LOCUS       sequence-215767-       11678 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TET-inducible expression of DVL2 protein with a deletion of its 
            DIX-domain .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11678)
  AUTHORS   Bocchi R, Egervari K, Carol-Perdiguer L, Viale B, Quairiaux C, De 
            Roo M, Boitard M, Oskouie S, Salmon P, Kiss JZ
  TITLE     Perturbed Wnt signaling leads to neuronal migration delay, altered 
            interhemispheric connections and impaired social behavior.
  JOURNAL   Nat Commun. 2017 Oct 27;8(1):1158. doi: 10.1038/s41467-017-01046-w.
  PUBMED    29079819
REFERENCE   2  (bases 1 to 11678)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-10-27"; volume: "8(1):1158. doi"; pages: " 
            10.1038/s41467-017-01046-w"
COMMENT     Sequence Label: pCWXPGR-pTF-DVL2deltaDIX
FEATURES             Location/Qualifiers
     source          1..11678
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             385..642
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    689..814
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1306..1423
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    1783..2016
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2201..2245
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     protein_bind    2522..2542
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    2569..2587
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    2610..2628
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    2774..2792
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    2854..2878
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2899..2913
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     protein_bind    3292..3303
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     protein_bind    complement(4870..4894)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4912..5422
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     5341..5359
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      5438..5447
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5444..5446,5447..5449,5450..6160)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5444..5446,5447..5449,5450..6160)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5489..5510)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5750..5769)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6097..6118
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             6176..6235
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             7304..7675
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     misc_feature    7695..8283
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7748..7768)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8166..8177)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8286..8302)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8287..8303)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    complement(8431..8464)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             8484..8664
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8702..8721)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    8767..8822
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8821..8840)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(9181..9197)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9181..9197)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9194..9216)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9205..9221
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9229..9235,9236..9253,9254..9259))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9274..9295
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        9354..9549
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9400..9535
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    9555..9689
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9592..9611)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9646..9665
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9757..9774)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9928..10516)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10008..10027)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10687..11478,11479..11547))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11310..11329
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11548..11652)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggaggcg tggcctgggc gggactgggg agtggcgagc
      421 cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc
      481 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
      541 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
      601 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
      661 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
      721 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
      781 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
      841 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
      901 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
      961 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     1021 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     1081 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaaaaa
     1141 agcacagcaa gcagcaggat cttcagacct ggaaattccc tacaatcccc aaagtcaagg
     1201 agtagtagaa tctatgaata aagaattaaa gaaaattata ggacaggtaa gagatcaggc
     1261 tgaacatctt aagacagcag tacaaatggc agtattcatc cacaatttta aaagaaaagg
     1321 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     1381 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     1441 cagcagaaat ccactttgga aaggaccagc aaagctcctc tggaaaggtg aaggggcagt
     1501 agtaatacaa gataatagtg acataaaagt agtgccaaga agaaaagcaa agatcattag
     1561 ggattatgga aaacagatgg caggtgatga ttgtgtggca agtagacagg atgaggatta
     1621 gaacatggaa aagtttagta aaacaccata aggaggagat atgagggaca attggagaag
     1681 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1741 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1801 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1861 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1921 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1981 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     2041 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     2101 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     2161 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     2221 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     2281 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     2341 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     2401 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     2461 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgctagt gattatcgga
     2521 tcaactttgt atagaaaagt tgggtacaac tcccactaga catatcctac tctatcattg
     2581 atagagtgac ccttccatag acatatccta ctctatcatt gatagagtga cccttccact
     2641 aggttcactt ttctctatca ttgataggga gtggtaaaca attgatggac aaaatctatc
     2701 aatgatagag agtgcatgtt actagtattg ttcttctcta tcattgatag tgactttgcc
     2761 taatcactta gcaactctat cattgataga gttctagtat tagcaaggcc tttcctctat
     2821 ataaggaagt tcatttcatt tggagaagat cccccaagtt tgtacaaaaa agcaggcttc
     2881 accatggact acaaagacga tgacgacgac aaggttacct ggcttgtgtc gtcagatacc
     2941 ccacagcctg aggtggctcc cccagcccat gagtctcgga cagaactggt tcctccgcct
     3001 ccacccttac cccctttgcc accagaaagg accagtggaa ttggggactc aaggcctcca
     3061 tccttccacc ctaatgtatc cagcagccat gaaaatctag agcctgagac agagaccgaa
     3121 tctgtcgtat cactgaggcg agaccgacct aggaggagag acagcagtga acatggcgct
     3181 ggtggccaca ggcctggtgg cccctcgagg ctggagcgcc acctggctgg ctacgagagc
     3241 tcttccaccc tcatgaccag tgagctggag agtaccagcc taggagactc ggatgaggat
     3301 gacaccatga gtaggttcag cagctccact gagcagagca gtgcctcccg cctcctcaag
     3361 cgccaccgaa ggcgaaggaa gcagcggccg ccacgcatgg agaggacctc atccttcagc
     3421 agtgtcaccg attccacaat gtctctcaac atcatcacgg tcacgctcaa catggagaag
     3481 tacaacttcc tgggcatctc cattgtgggc caaagtaacg agcgtggtga tgggggcatc
     3541 tacatcggct ccatcatgaa agggggcgcg gtggctgcgg acgggcggat cgagcctgga
     3601 gacatgcttt tgcaggtaaa tgatatgaac tttgagaaca tgagcaacga cgatgctgta
     3661 cgagtgctga gagacatcgt gcacaagcca ggccccatcg tgctgaccgt ggccaagtgt
     3721 tgggacccgt ctccccaggc ctacttcacc ctccctcgaa atgagcccat ccagcccatc
     3781 gacccagccg cctgggtgtc gcactcggct gcgctgactg gcgccttccc tgcttaccct
     3841 ggctcctcat ctatgagcac tatcacatct ggctcctctc tgcctgatgg ctgcgaaggc
     3901 cggggtctct ctgtccacat ggacatggcc tctgtcacca aggccatggc agccccagag
     3961 tctgggctcg aagtccggga ccgcatgtgg ctcaagatca ccatcccaaa cgcctttcta
     4021 ggctccgatg tggtggactg gctgtaccat cacgtggaag gttttcctga gcgccgggag
     4081 gcccgcaagt atgccagtgg gctgttgaag gcgggactca tccggcacac cgtcaacaag
     4141 attactttct ctgagcagtg ctattatgtc ttcggggacc tcagtggtgg ctgtgagagt
     4201 tacctagtta acctctctct gaatgacaat gacggttcca gtggggcttc agaccaggat
     4261 accctggcac ctctgcctgg agctactccc tggcccctgc tgcccacctt ctcctaccag
     4321 tatccagcgc cacaccccta cagcccccag cctccaccct accacgagct ttcttcgtac
     4381 acctatggtg gaggcagtgc cagcagccag cacagtgagg ggagccggag cagcgggtcg
     4441 acaagaagcg atgggggggc tgggcgcaca ggcaggcctg aggaacgggc ccctgagtcc
     4501 aagtccggca gtggcagtga gtcagaactc tccagccggg gaggcagcct tcggcggggt
     4561 ggggagcctg gtgggactgg tgatggcggc cctcctccat ccaggggctc gacaggcgct
     4621 cctcctaatc tccgagctct tcccgggctc catccctatg gagccccatc tggcatggct
     4681 ctcccctata accccatgat ggtagttatg atgcctccac ccccaccccc tgtctccaca
     4741 gcagtgcagc cccctggtgc ccctccagtc agagacctgg gctccgtgcc cccagaactg
     4801 acagctagcc gtcagagctt ccacatggcc atgggcaacc ccagtgagtt ttttgtggat
     4861 gttatgtaga cccagctttc ttgtacaaag tggtgataat cgaattccca cggggttggg
     4921 gttgcgcctt ttccaaggca gccctgggtt tgcgcaggga cgcggctgct ctgggcgtgg
     4981 ttccgggaaa cgcagcggcg ccgaccctgg gtctcgcaca ttcttcacgt ccgttcgcag
     5041 cgtcacccgg atcttcgccg ctacccttgt gggccccccg gcgacgcttc ctgctccgcc
     5101 cctaagtcgg gaaggttcct tgcggttcgc ggcgtgccgg acgtgacaaa cggaagccgc
     5161 acgtctcact agtaccctcg cagacggaca gcgccaggga gcaatggcag cgcgccgacc
     5221 gcgatgggct gtggccaata gcggctgctc agcggggcgc gccgagagca gcggccggga
     5281 aggggcggtg cgggaggcgg ggtgtggggc ggtagtgtgg gccctgttcc tgcccgcgcg
     5341 gtgttccgca ttctgcaagc ctccggagcg cacgtcggca gtcggctccc tcgttgaccg
     5401 aatcaccgac ctctctcccc agggggatcc accggtcgcc accatggtga gcaagggcga
     5461 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     5521 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     5581 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     5641 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     5701 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     5761 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     5821 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     5881 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     5941 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     6001 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     6061 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     6121 cgccgccggg atcactctcg gcatggacga gctgtacaag gctagcggat caggtcaatg
     6181 tactaactac gctttgttga agcttgccgg cgatgttgaa agtaaccccg gtcctggatc
     6241 catgtccaga ctggacaaga gcaaagtcat aaacggcgct ctggaattac tcaatggagt
     6301 cggtatcgaa ggcctgacga caaggaaact cgctcaaaag ctgggagttg agcagcctac
     6361 cctgtactgg cacgtgaaga acaagcgggc cctgctcgat gccctgccaa tcgagatgct
     6421 ggacaggcat catacccact tctgccccct ggaaggcgag tcatggcaag actttctgcg
     6481 gaacaacgcc aagtcattcc gctgtgctct cctctcacat cgcgacgggg ctaaagtgca
     6541 tctcggcacc cgcccaacag agaaacagta cgaaaccctg gaaaatcagc tcgcgttcct
     6601 gtgtcagcaa ggcttctccc tggagaacgc actgtacgct ctgtccgccg tgggccactt
     6661 tacactgggc tgcgtattgg aggaacagga gcatcaagta gcaaaagagg aaagagagac
     6721 acctaccacc gattctatgc ccccacttct gagacaagca attgagctgt tcgaccggca
     6781 gggagccgaa cctgccttcc ttttcggcct ggaactaatc atatgtggcc tggagaaaca
     6841 gctaaagtgc gaaagcggcg ggccggcctc gagcgaattc cagtacctgc cagatacaga
     6901 cgatcgtcac cggattgagg agaaacgtaa aaggacatat gagaccttca agagcatcat
     6961 gaagaagagt cctttcagcg gacccaccga cccccggcct ccacctcgac gcattgctgt
     7021 gccttcccgc agctcagctt ctgtccccaa gccagcaccc cagccctatc cctttacgtc
     7081 atccctgagc accatcaact atgatgagtt tcccaccatg gtgtttcctt ctgggcagat
     7141 cagccaggcc tcggccttgg ccccggcccc tccccaagtc ctgccccagg ctccagcccc
     7201 tgcccctgct ccagccatgg tatcagctct ggcccaggcc ccagcccctg tcccagtcct
     7261 agccccaggc cctcctcagg ctgtggcccc acctgccccc aagcccaccc aggctgggga
     7321 aggaacgctg tcagaggccc tgctgcagct gcagtttgat gatgaagacc tgggggcctt
     7381 gcttggcaac agcacagacc cagctgtgtt cacagacctg gcatccgtcg acaactccga
     7441 gtttcagcag ctgctgaacc agggcatacc tgtggccccc cacacaactg agcccatgct
     7501 gatggagtac cctgaggcta taactcgcct agtgacaggg gcccagaggc cccccgaccc
     7561 agctcctgct ccactggggg ccccggggct ccccaatggc ctcctttcag gagatgaaga
     7621 cttctcctcc attgcggaca tggacttctc agccctgctg agtcagatca gctcctaaga
     7681 tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     7741 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     7801 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     7861 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     7921 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     7981 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     8041 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     8101 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     8161 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     8221 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     8281 cgcatcgata ccgtcgacct cgaggttcgc tttaagacca atgacttaca aggcagctgt
     8341 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     8401 aagacaagat gggatcaatt caccatggga ataacttcgt atagcataca ttatacgaag
     8461 ttatgctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     8521 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     8581 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     8641 agtcagtgtg gaaaatctct agcagcatct agttgaatcg ccagtgtgtc gagctagcag
     8701 atctttttcc ctctgccaaa aattatgggg acatcatgaa gccccttgag catctgactt
     8761 ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct
     8821 cactcggaag gacatatggg agggcaaatc atttaaaaca tcagaatgag tatttggttt
     8881 agagtttggc aacatatgcc atatgctggc tgccatgaac aaaggtggct ataaagaggt
     8941 catcagtata tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact
     9001 tgaggttaga ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa
     9061 ttttccttac atgttttact agccagattt ttcctcctct cctgactact cccagtcata
     9121 gctgtccctc ttctcttatg aagatccctc gacctgcagc ccaagcttgg cgtaatcatg
     9181 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     9241 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     9301 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagcgga tccgcatctc
     9361 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     9421 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     9481 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     9541 ttttgcaaaa agctaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     9601 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     9661 tcatcaatgt atcttatcat gtctggatcc gctgcattaa tgaatcggcc aacgcgcggg
     9721 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     9781 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     9841 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     9901 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     9961 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
    10021 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
    10081 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta
    10141 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
    10201 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
    10261 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
    10321 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
    10381 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
    10441 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
    10501 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
    10561 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
    10621 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
    10681 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
    10741 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
    10801 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
    10861 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
    10921 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
    10981 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
    11041 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
    11101 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
    11161 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
    11221 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
    11281 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
    11341 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
    11401 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
    11461 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
    11521 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
    11581 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
    11641 aggggttccg cgcacatttc cccgaaaagt gccacctg
//
