LOCUS       sequence-215768-       11435 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TET-inducible expression of a dominant negative form of the TCF4 
            transcription factor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11435)
  AUTHORS   Bocchi R, Egervari K, Carol-Perdiguer L, Viale B, Quairiaux C, De 
            Roo M, Boitard M, Oskouie S, Salmon P, Kiss JZ
  TITLE     Perturbed Wnt signaling leads to neuronal migration delay, altered 
            interhemispheric connections and impaired social behavior.
  JOURNAL   Nat Commun. 2017 Oct 27;8(1):1158. doi: 10.1038/s41467-017-01046-w.
  PUBMED    29079819
REFERENCE   2  (bases 1 to 11435)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-10-27"; volume: "8(1):1158. doi"; pages: " 
            10.1038/s41467-017-01046-w"
COMMENT     Sequence Label: pCWXPGR-pTF-dnTCF4
FEATURES             Location/Qualifiers
     source          1..11435
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             385..642
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    689..814
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1306..1423
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    1783..2016
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2201..2245
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     protein_bind    2522..2542
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    2569..2587
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    2610..2628
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    2774..2792
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    2854..2878
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2887..2910
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(4627..4651)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4669..5179
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     5098..5116
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      5195..5204
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5201..5203,5204..5206,5207..5917)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5201..5203,5204..5206,5207..5917)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5246..5267)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5507..5526)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5854..5875
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5933..5992
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             7061..7432
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     misc_feature    7452..8040
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7505..7525)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7923..7934)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8043..8059)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8044..8060)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    complement(8188..8221)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             8241..8421
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8459..8478)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    8524..8579
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8578..8597)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(8938..8954)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8938..8954)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8951..8973)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8962..8978
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8986..8992,8993..9010,9011..9016))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9031..9052
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        9111..9306
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9157..9292
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    9312..9446
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9349..9368)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9403..9422
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9514..9531)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9685..10273)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9765..9784)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10444..11235,11236..11304))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11067..11086
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11305..11409)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggaggcg tggcctgggc gggactgggg agtggcgagc
      421 cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc
      481 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
      541 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
      601 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
      661 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
      721 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
      781 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
      841 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
      901 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
      961 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     1021 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     1081 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaaaaa
     1141 agcacagcaa gcagcaggat cttcagacct ggaaattccc tacaatcccc aaagtcaagg
     1201 agtagtagaa tctatgaata aagaattaaa gaaaattata ggacaggtaa gagatcaggc
     1261 tgaacatctt aagacagcag tacaaatggc agtattcatc cacaatttta aaagaaaagg
     1321 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     1381 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     1441 cagcagaaat ccactttgga aaggaccagc aaagctcctc tggaaaggtg aaggggcagt
     1501 agtaatacaa gataatagtg acataaaagt agtgccaaga agaaaagcaa agatcattag
     1561 ggattatgga aaacagatgg caggtgatga ttgtgtggca agtagacagg atgaggatta
     1621 gaacatggaa aagtttagta aaacaccata aggaggagat atgagggaca attggagaag
     1681 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1741 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1801 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1861 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1921 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1981 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     2041 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     2101 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     2161 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     2221 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     2281 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     2341 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     2401 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     2461 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgctagt gattatcgga
     2521 tcaactttgt atagaaaagt tgggtacaac tcccactaga catatcctac tctatcattg
     2581 atagagtgac ccttccatag acatatccta ctctatcatt gatagagtga cccttccact
     2641 aggttcactt ttctctatca ttgataggga gtggtaaaca attgatggac aaaatctatc
     2701 aatgatagag agtgcatgtt actagtattg ttcttctcta tcattgatag tgactttgcc
     2761 taatcactta gcaactctat cattgataga gttctagtat tagcaaggcc tttcctctat
     2821 ataaggaagt tcatttcatt tggagaagat cccccaagtt tgtacaaaaa agcaggcttc
     2881 accatggact acaaagacga tgacgataaa aaggatccga gctcgaattc cgaaaactcc
     2941 tcggcagaga gggatttagc tgatgtcaaa tcgtctctag tcaatgaatc agaaacgaat
     3001 caaaacagct cctccgattc cgaggcggaa agacggcctc cgcctcgctc cgaaagtttc
     3061 cgagacaaat cccgggaaag tttggaagaa gcggccaaga ggcaagatgg agggctcttt
     3121 aaggggccac cgtatcccgg ctaccccttc atcatgatcc ccgacctgac gagcccctac
     3181 ctccccaacg gatcgctctc gcccaccgcc cgaacctatc tccagatgaa atggccactg
     3241 cttgatgtcc aggcagggag cctccagagt agacaagccc tcaaggatgc ccggtcccca
     3301 tcaccggcac acattgtctc taacaaagtg ccagtggtgc agcaccctca ccatgtccac
     3361 cccctcacgc ctcttatcac gtacagcaat gaacacttca cgccgggaaa cccacctcca
     3421 cacttaccag ccgacgtaga ccccaaaaca ggaatcccac ggcctccgca ccctccagat
     3481 atatccccgt attacccact atcgcctggc accgtaggac aaatccccca tccgctagga
     3541 tggttagtac cacagcaagg tcaaccagtg tacccaatca cgacaggagg attcagacac
     3601 ccctacccca cagctctgac cgtcaatgct tccatgtcca ggttccctcc ccatatggtc
     3661 ccaccacatc atacgctaca cacgacgggc attccgcatc cggccatagt cacaccaaca
     3721 gtcaaacagg aatcgtccca gagtgatgtc ggctcactcc atagttcaaa gcatcaggac
     3781 tccaaaaagg aagaagaaaa gaagaagccc cacataaaga aacctcttaa tgcattcatg
     3841 ttgtatatga aggaaatgag agcaaaggtc gtagctgagt gcacgttgaa agaaagcgcg
     3901 gccatcaacc agatccttgg gcggaggtgg catgcactgt ccagagaaga gcaagcgaaa
     3961 tactacgagc tggcccggaa ggagcgacag cttcatatgc aactgtaccc cggctggtcc
     4021 gcgcgggata actatggaaa gaagaagaag aggaaaaggg acaagcagcc gggagagacc
     4081 aatgaacaca gcgaatgttt cctaaatcct tgcctttcac ttcctccgat tacagacctg
     4141 agcgctccta agaaatgccg agcgcgcttt ggccttgatc aacagaataa ctggtgcggc
     4201 ccttgcagga gaaaaaaaaa gtgcgttcgc tacatacaag gtgaaggcag ctgcctcagc
     4261 ccaccctctt cagatggaag cttactagat tcgcctcccc cctccccgaa cctgctaggc
     4321 tcccctcccc gagacgccaa gtcacagact gagcagaccc agcctctgtc gctgtccctg
     4381 aagcccgacc ccctggccca cctgtccatg atgcctccgc cacccgccct cctgctcgct
     4441 gaggccaccc acaaggcctc cgccctctgt cccaacgggg ccctggacct gcccccagcc
     4501 gctttgcagc ctgccgcccc ctcctcatca attgcacagc cgtcgacttc ttccttacat
     4561 tcccacagct ccctggccgg gacccagccc cagccgctgt cgctcgtcac caagtcttta
     4621 gaatagaccc agctttcttg tacaaagtgg tgataatcga attcccacgg ggttggggtt
     4681 gcgccttttc caaggcagcc ctgggtttgc gcagggacgc ggctgctctg ggcgtggttc
     4741 cgggaaacgc agcggcgccg accctgggtc tcgcacattc ttcacgtccg ttcgcagcgt
     4801 cacccggatc ttcgccgcta cccttgtggg ccccccggcg acgcttcctg ctccgcccct
     4861 aagtcgggaa ggttccttgc ggttcgcggc gtgccggacg tgacaaacgg aagccgcacg
     4921 tctcactagt accctcgcag acggacagcg ccagggagca atggcagcgc gccgaccgcg
     4981 atgggctgtg gccaatagcg gctgctcagc ggggcgcgcc gagagcagcg gccgggaagg
     5041 ggcggtgcgg gaggcggggt gtggggcggt agtgtgggcc ctgttcctgc ccgcgcggtg
     5101 ttccgcattc tgcaagcctc cggagcgcac gtcggcagtc ggctccctcg ttgaccgaat
     5161 caccgacctc tctccccagg gggatccacc ggtcgccacc atggtgagca agggcgagga
     5221 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     5281 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     5341 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
     5401 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     5461 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     5521 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     5581 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     5641 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     5701 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     5761 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     5821 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     5881 cgccgggatc actctcggca tggacgagct gtacaaggct agcggatcag gtcaatgtac
     5941 taactacgct ttgttgaagc ttgccggcga tgttgaaagt aaccccggtc ctggatccat
     6001 gtccagactg gacaagagca aagtcataaa cggcgctctg gaattactca atggagtcgg
     6061 tatcgaaggc ctgacgacaa ggaaactcgc tcaaaagctg ggagttgagc agcctaccct
     6121 gtactggcac gtgaagaaca agcgggccct gctcgatgcc ctgccaatcg agatgctgga
     6181 caggcatcat acccacttct gccccctgga aggcgagtca tggcaagact ttctgcggaa
     6241 caacgccaag tcattccgct gtgctctcct ctcacatcgc gacggggcta aagtgcatct
     6301 cggcacccgc ccaacagaga aacagtacga aaccctggaa aatcagctcg cgttcctgtg
     6361 tcagcaaggc ttctccctgg agaacgcact gtacgctctg tccgccgtgg gccactttac
     6421 actgggctgc gtattggagg aacaggagca tcaagtagca aaagaggaaa gagagacacc
     6481 taccaccgat tctatgcccc cacttctgag acaagcaatt gagctgttcg accggcaggg
     6541 agccgaacct gccttccttt tcggcctgga actaatcata tgtggcctgg agaaacagct
     6601 aaagtgcgaa agcggcgggc cggcctcgag cgaattccag tacctgccag atacagacga
     6661 tcgtcaccgg attgaggaga aacgtaaaag gacatatgag accttcaaga gcatcatgaa
     6721 gaagagtcct ttcagcggac ccaccgaccc ccggcctcca cctcgacgca ttgctgtgcc
     6781 ttcccgcagc tcagcttctg tccccaagcc agcaccccag ccctatccct ttacgtcatc
     6841 cctgagcacc atcaactatg atgagtttcc caccatggtg tttccttctg ggcagatcag
     6901 ccaggcctcg gccttggccc cggcccctcc ccaagtcctg ccccaggctc cagcccctgc
     6961 ccctgctcca gccatggtat cagctctggc ccaggcccca gcccctgtcc cagtcctagc
     7021 cccaggccct cctcaggctg tggccccacc tgcccccaag cccacccagg ctggggaagg
     7081 aacgctgtca gaggccctgc tgcagctgca gtttgatgat gaagacctgg gggccttgct
     7141 tggcaacagc acagacccag ctgtgttcac agacctggca tccgtcgaca actccgagtt
     7201 tcagcagctg ctgaaccagg gcatacctgt ggccccccac acaactgagc ccatgctgat
     7261 ggagtaccct gaggctataa ctcgcctagt gacaggggcc cagaggcccc ccgacccagc
     7321 tcctgctcca ctgggggccc cggggctccc caatggcctc ctttcaggag atgaagactt
     7381 ctcctccatt gcggacatgg acttctcagc cctgctgagt cagatcagct cctaagatca
     7441 agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     7501 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     7561 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     7621 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     7681 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     7741 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     7801 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     7861 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     7921 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     7981 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     8041 atcgataccg tcgacctcga ggttcgcttt aagaccaatg acttacaagg cagctgtaga
     8101 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     8161 acaagatggg atcaattcac catgggaata acttcgtata gcatacatta tacgaagtta
     8221 tgctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     8281 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     8341 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     8401 cagtgtggaa aatctctagc agcatctagt tgaatcgcca gtgtgtcgag ctagcagatc
     8461 tttttccctc tgccaaaaat tatggggaca tcatgaagcc ccttgagcat ctgacttctg
     8521 gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac
     8581 tcggaaggac atatgggagg gcaaatcatt taaaacatca gaatgagtat ttggtttaga
     8641 gtttggcaac atatgccata tgctggctgc catgaacaaa ggtggctata aagaggtcat
     8701 cagtatatga aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga
     8761 ggttagattt tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt
     8821 tccttacatg ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct
     8881 gtccctcttc tcttatgaag atccctcgac ctgcagccca agcttggcgt aatcatggtc
     8941 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     9001 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     9061 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagcggatcc gcatctcaat
     9121 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     9181 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     9241 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     9301 tgcaaaaagc taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     9361 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     9421 tcaatgtatc ttatcatgtc tggatccgct gcattaatga atcggccaac gcgcggggag
     9481 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     9541 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     9601 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     9661 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     9721 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     9781 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     9841 gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct
     9901 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     9961 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
    10021 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
    10081 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
    10141 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
    10201 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
    10261 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
    10321 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
    10381 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
    10441 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
    10501 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
    10561 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
    10621 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
    10681 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
    10741 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
    10801 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
    10861 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
    10921 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
    10981 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
    11041 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
    11101 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
    11161 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
    11221 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
    11281 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
    11341 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
    11401 ggttccgcgc acatttcccc gaaaagtgcc acctg
//
