LOCUS       sequence-215769-       10766 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TET-inducible expression of the NeuroD1 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10766)
  TITLE     Salmon Lab plasmids submitted
REFERENCE   2  (bases 1 to 10766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCWXPGR-pTF-NeuroD1
FEATURES             Location/Qualifiers
     source          1..10766
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             385..642
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    689..814
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1306..1423
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    1783..2016
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2201..2245
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     protein_bind    2522..2542
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    2569..2587
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    2610..2628
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    2774..2792
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    2854..2878
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3958..3982)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4000..4510
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4429..4447
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      4526..4535
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4532..4534,4535..4537,4538..5248)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4532..4534,4535..4537,4538..5248)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4577..4598)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4838..4857)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5185..5206
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5264..5323
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             6392..6763
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     misc_feature    6783..7371
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6836..6856)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7254..7265)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7374..7390)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7375..7391)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    complement(7519..7552)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             7572..7752
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7790..7809)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7855..7910
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7909..7928)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(8269..8285)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8269..8285)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8282..8304)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8293..8309
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8317..8323,8324..8341,8342..8347))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8362..8383
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8442..8637
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8488..8623
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    8643..8777
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8680..8699)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8734..8753
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8845..8862)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9016..9604)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9096..9115)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9775..10566,10567..10635))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10398..10417
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10636..10740)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggaggcg tggcctgggc gggactgggg agtggcgagc
      421 cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc
      481 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
      541 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
      601 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
      661 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
      721 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
      781 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
      841 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
      901 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
      961 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     1021 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     1081 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaaaaa
     1141 agcacagcaa gcagcaggat cttcagacct ggaaattccc tacaatcccc aaagtcaagg
     1201 agtagtagaa tctatgaata aagaattaaa gaaaattata ggacaggtaa gagatcaggc
     1261 tgaacatctt aagacagcag tacaaatggc agtattcatc cacaatttta aaagaaaagg
     1321 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     1381 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     1441 cagcagaaat ccactttgga aaggaccagc aaagctcctc tggaaaggtg aaggggcagt
     1501 agtaatacaa gataatagtg acataaaagt agtgccaaga agaaaagcaa agatcattag
     1561 ggattatgga aaacagatgg caggtgatga ttgtgtggca agtagacagg atgaggatta
     1621 gaacatggaa aagtttagta aaacaccata aggaggagat atgagggaca attggagaag
     1681 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1741 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1801 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1861 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1921 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1981 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     2041 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     2101 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     2161 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     2221 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     2281 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     2341 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     2401 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     2461 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgctagt gattatcgga
     2521 tcaactttgt atagaaaagt tgggtacaac tcccactaga catatcctac tctatcattg
     2581 atagagtgac ccttccatag acatatccta ctctatcatt gatagagtga cccttccact
     2641 aggttcactt ttctctatca ttgataggga gtggtaaaca attgatggac aaaatctatc
     2701 aatgatagag agtgcatgtt actagtattg ttcttctcta tcattgatag tgactttgcc
     2761 taatcactta gcaactctat cattgataga gttctagtat tagcaaggcc tttcctctat
     2821 ataaggaagt tcatttcatt tggagaagat cccccaagtt tgtacaaaaa agcaggcttc
     2881 accatgacca aatcatacag cgagagcggg ctgatgggcg agcctcagcc ccaaggtccc
     2941 ccaagctgga cagatgagtg tctcagttct caggacgagg aacacgaggc agacaagaaa
     3001 gaggacgagc ttgaagccat gaatgcagag gaggactctc tgagaaacgg gggagaggag
     3061 gaggaggaag atgaggatct agaggaagag gaggaagaag aagaggagga ggaggatcaa
     3121 aagcccaaga gacggggtcc caaaaagaaa aagatgacca aggcgcgcct agaacgtttt
     3181 aaattaaggc gcatgaaggc caacgcccgc gagcggaacc gcatgcacgg gctgaacgcg
     3241 gcgctggaca acctgcgcaa ggtggtacct tgctactcca agacccagaa actgtctaaa
     3301 atagagacac tgcgcttggc caagaactac atctgggctc tgtcagagat cctgcgctca
     3361 ggcaaaagcc ctgatctggt ctccttcgta cagacgctct gcaaaggttt gtcccagccc
     3421 actaccaatt tggtcgccgg ctgcctgcag ctcaaccctc ggactttctt gcctgagcag
     3481 aacccggaca tgcccccgca tctgccaacc gccagcgctt ccttcccggt gcatccctac
     3541 tcctaccagt cccctggact gcccagcccg ccctacggca ccatggacag ctcccacgtc
     3601 ttccacgtca agccgccgcc acacgcctac agcgcagctc tggagccctt ctttgaaagc
     3661 cccctaactg actgcaccag cccttccttt gacggacccc tcagcccgcc gctcagcatc
     3721 aatggcaact tctctttcaa acacgaacca tccgccgagt ttgaaaaaaa ttatgccttt
     3781 accatgcact accctgcagc gacgctggca gggccccaaa gccacggatc aatcttctct
     3841 tccggtgccg ctgcccctcg ctgcgagatc cccatagaca acattatgtc tttcgatagc
     3901 cattcgcatc atgagcgagt catgagtgcc cagcttaatg ccatctttca cgattagacc
     3961 cagctttctt gtacaaagtg gtgataatcg aattcccacg gggttggggt tgcgcctttt
     4021 ccaaggcagc cctgggtttg cgcagggacg cggctgctct gggcgtggtt ccgggaaacg
     4081 cagcggcgcc gaccctgggt ctcgcacatt cttcacgtcc gttcgcagcg tcacccggat
     4141 cttcgccgct acccttgtgg gccccccggc gacgcttcct gctccgcccc taagtcggga
     4201 aggttccttg cggttcgcgg cgtgccggac gtgacaaacg gaagccgcac gtctcactag
     4261 taccctcgca gacggacagc gccagggagc aatggcagcg cgccgaccgc gatgggctgt
     4321 ggccaatagc ggctgctcag cggggcgcgc cgagagcagc ggccgggaag gggcggtgcg
     4381 ggaggcgggg tgtggggcgg tagtgtgggc cctgttcctg cccgcgcggt gttccgcatt
     4441 ctgcaagcct ccggagcgca cgtcggcagt cggctccctc gttgaccgaa tcaccgacct
     4501 ctctccccag ggggatccac cggtcgccac catggtgagc aagggcgagg agctgttcac
     4561 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     4621 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     4681 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca
     4741 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     4801 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     4861 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     4921 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     4981 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
     5041 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     5101 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa
     5161 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     5221 cactctcggc atggacgagc tgtacaaggc tagcggatca ggtcaatgta ctaactacgc
     5281 tttgttgaag cttgccggcg atgttgaaag taaccccggt cctggatcca tgtccagact
     5341 ggacaagagc aaagtcataa acggcgctct ggaattactc aatggagtcg gtatcgaagg
     5401 cctgacgaca aggaaactcg ctcaaaagct gggagttgag cagcctaccc tgtactggca
     5461 cgtgaagaac aagcgggccc tgctcgatgc cctgccaatc gagatgctgg acaggcatca
     5521 tacccacttc tgccccctgg aaggcgagtc atggcaagac tttctgcgga acaacgccaa
     5581 gtcattccgc tgtgctctcc tctcacatcg cgacggggct aaagtgcatc tcggcacccg
     5641 cccaacagag aaacagtacg aaaccctgga aaatcagctc gcgttcctgt gtcagcaagg
     5701 cttctccctg gagaacgcac tgtacgctct gtccgccgtg ggccacttta cactgggctg
     5761 cgtattggag gaacaggagc atcaagtagc aaaagaggaa agagagacac ctaccaccga
     5821 ttctatgccc ccacttctga gacaagcaat tgagctgttc gaccggcagg gagccgaacc
     5881 tgccttcctt ttcggcctgg aactaatcat atgtggcctg gagaaacagc taaagtgcga
     5941 aagcggcggg ccggcctcga gcgaattcca gtacctgcca gatacagacg atcgtcaccg
     6001 gattgaggag aaacgtaaaa ggacatatga gaccttcaag agcatcatga agaagagtcc
     6061 tttcagcgga cccaccgacc cccggcctcc acctcgacgc attgctgtgc cttcccgcag
     6121 ctcagcttct gtccccaagc cagcacccca gccctatccc tttacgtcat ccctgagcac
     6181 catcaactat gatgagtttc ccaccatggt gtttccttct gggcagatca gccaggcctc
     6241 ggccttggcc ccggcccctc cccaagtcct gccccaggct ccagcccctg cccctgctcc
     6301 agccatggta tcagctctgg cccaggcccc agcccctgtc ccagtcctag ccccaggccc
     6361 tcctcaggct gtggccccac ctgcccccaa gcccacccag gctggggaag gaacgctgtc
     6421 agaggccctg ctgcagctgc agtttgatga tgaagacctg ggggccttgc ttggcaacag
     6481 cacagaccca gctgtgttca cagacctggc atccgtcgac aactccgagt ttcagcagct
     6541 gctgaaccag ggcatacctg tggcccccca cacaactgag cccatgctga tggagtaccc
     6601 tgaggctata actcgcctag tgacaggggc ccagaggccc cccgacccag ctcctgctcc
     6661 actgggggcc ccggggctcc ccaatggcct cctttcagga gatgaagact tctcctccat
     6721 tgcggacatg gacttctcag ccctgctgag tcagatcagc tcctaagatc aagcttatcg
     6781 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     6841 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     6901 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     6961 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     7021 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     7081 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     7141 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     7201 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     7261 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     7321 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc
     7381 gtcgacctcg aggttcgctt taagaccaat gacttacaag gcagctgtag atcttagcca
     7441 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatgg
     7501 gatcaattca ccatgggaat aacttcgtat agcatacatt atacgaagtt atgctgcttt
     7561 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     7621 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     7681 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     7741 aaatctctag cagcatctag ttgaatcgcc agtgtgtcga gctagcagat ctttttccct
     7801 ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa
     7861 ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga
     7921 catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa
     7981 catatgccat atgctggctg ccatgaacaa aggtggctat aaagaggtca tcagtatatg
     8041 aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg aggttagatt
     8101 ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt ttccttacat
     8161 gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc tgtccctctt
     8221 ctcttatgaa gatccctcga cctgcagccc aagcttggcg taatcatggt catagctgtt
     8281 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     8341 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     8401 gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc cgcatctcaa ttagtcagca
     8461 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     8521 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     8581 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     8641 ctaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     8701 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     8761 cttatcatgt ctggatccgc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     8821 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     8881 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     8941 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     9001 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     9061 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     9121 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     9181 tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc tcagttcggt
     9241 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     9301 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     9361 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     9421 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     9481 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     9541 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     9601 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     9661 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     9721 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     9781 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     9841 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     9901 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     9961 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
    10021 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
    10081 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
    10141 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
    10201 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
    10261 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
    10321 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
    10381 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
    10441 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
    10501 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
    10561 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
    10621 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
    10681 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
    10741 cacatttccc cgaaaagtgc cacctg
//
