LOCUS       sequence-215771-       10484 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TET-inducible expression of the Ngn2 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10484)
  TITLE     Salmon Lab plasmids submitted
REFERENCE   2  (bases 1 to 10484)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCWXPGR-pTF-Ngn2
FEATURES             Location/Qualifiers
     source          1..10484
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             385..642
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    689..814
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1306..1423
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    1783..2016
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2201..2245
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     protein_bind    2522..2542
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    2569..2587
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    2610..2628
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    2774..2792
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    2854..2878
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3676..3700)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        3718..4228
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4147..4165
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      4244..4253
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4250..4252,4253..4255,4256..4966)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4250..4252,4253..4255,4256..4966)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4295..4316)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4556..4575)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4903..4924
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4982..5041
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             6110..6481
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     misc_feature    6501..7089
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6554..6574)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6972..6983)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7092..7108)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7093..7109)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    complement(7237..7270)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             7290..7470
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7508..7527)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7573..7628
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7627..7646)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7987..8003)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7987..8003)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8000..8022)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8011..8027
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8035..8041,8042..8059,8060..8065))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8080..8101
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8160..8355
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8206..8341
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    8361..8495
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8398..8417)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8452..8471
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8563..8580)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8734..9322)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8814..8833)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9493..10284,10285..10353))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10116..10135
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10354..10458)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggaggcg tggcctgggc gggactgggg agtggcgagc
      421 cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc
      481 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
      541 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
      601 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
      661 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
      721 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
      781 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
      841 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
      901 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
      961 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     1021 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     1081 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaaaaa
     1141 agcacagcaa gcagcaggat cttcagacct ggaaattccc tacaatcccc aaagtcaagg
     1201 agtagtagaa tctatgaata aagaattaaa gaaaattata ggacaggtaa gagatcaggc
     1261 tgaacatctt aagacagcag tacaaatggc agtattcatc cacaatttta aaagaaaagg
     1321 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     1381 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     1441 cagcagaaat ccactttgga aaggaccagc aaagctcctc tggaaaggtg aaggggcagt
     1501 agtaatacaa gataatagtg acataaaagt agtgccaaga agaaaagcaa agatcattag
     1561 ggattatgga aaacagatgg caggtgatga ttgtgtggca agtagacagg atgaggatta
     1621 gaacatggaa aagtttagta aaacaccata aggaggagat atgagggaca attggagaag
     1681 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1741 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1801 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1861 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1921 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1981 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     2041 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     2101 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     2161 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     2221 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     2281 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     2341 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     2401 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     2461 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgctagt gattatcgga
     2521 tcaactttgt atagaaaagt tgggtacaac tcccactaga catatcctac tctatcattg
     2581 atagagtgac ccttccatag acatatccta ctctatcatt gatagagtga cccttccact
     2641 aggttcactt ttctctatca ttgataggga gtggtaaaca attgatggac aaaatctatc
     2701 aatgatagag agtgcatgtt actagtattg ttcttctcta tcattgatag tgactttgcc
     2761 taatcactta gcaactctat cattgataga gttctagtat tagcaaggcc tttcctctat
     2821 ataaggaagt tcatttcatt tggagaagat cccccaagtt tgtacaaaaa agcaggcttc
     2881 accatgttcg tcaaatctga gactctggag ttgaaggagg aagaggaggt actgatgctg
     2941 ctgggctcgg cttccccggc ctcggcgacc ctgaccccga tgtcctccag cgcggacgag
     3001 gaggaggacg aggagctgcg ccggccgggc tccgcgcgtg ggcagcgtgg agcggaagcc
     3061 gggcaggggg tgcagggcag tccggcgtcg ggtgccgggg gttgccggcc agggcggctg
     3121 ctgggcctga tgcacgagtg caagcgtcgc ccgtcgcgct cacgggccgt ctcccgaggt
     3181 gccaagacgg cggagacggt gcagcgcatc aagaagaccc gcaggctcaa ggccaacaac
     3241 cgcgagcgca accgcatgca caacctaaac gccgcgctgg acgcgctgcg cgaggtgctg
     3301 cccaccttcc ccgaggatgc caagctcacg aagatcgaga cgctgcgctt cgcccacaat
     3361 tacatctggg cgctcaccga gactctgcgc ctggcggacc actgcgccgg cgccggtggc
     3421 ctccaggggg cgctcttcac ggaggcggtg ctcctgagcc cgggagctgc gctcggcgcc
     3481 agcggggaca gcccttctcc accttcctcc tggagctgca ccaacagccc ggcgtcatcc
     3541 tccaactcca cgtccccata cagctgcact ttatcgcccg ctagccccgg gtcagacgtg
     3601 gactactggc agcccccacc tccggagaag catcgttatg cgcctcacct gcccctcgcc
     3661 agggactgta tctagaccca gctttcttgt acaaagtggt gataatcgaa ttcccacggg
     3721 gttggggttg cgccttttcc aaggcagccc tgggtttgcg cagggacgcg gctgctctgg
     3781 gcgtggttcc gggaaacgca gcggcgccga ccctgggtct cgcacattct tcacgtccgt
     3841 tcgcagcgtc acccggatct tcgccgctac ccttgtgggc cccccggcga cgcttcctgc
     3901 tccgccccta agtcgggaag gttccttgcg gttcgcggcg tgccggacgt gacaaacgga
     3961 agccgcacgt ctcactagta ccctcgcaga cggacagcgc cagggagcaa tggcagcgcg
     4021 ccgaccgcga tgggctgtgg ccaatagcgg ctgctcagcg gggcgcgccg agagcagcgg
     4081 ccgggaaggg gcggtgcggg aggcggggtg tggggcggta gtgtgggccc tgttcctgcc
     4141 cgcgcggtgt tccgcattct gcaagcctcc ggagcgcacg tcggcagtcg gctccctcgt
     4201 tgaccgaatc accgacctct ctccccaggg ggatccaccg gtcgccacca tggtgagcaa
     4261 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     4321 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     4381 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     4441 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
     4501 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     4561 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     4621 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     4681 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
     4741 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
     4801 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
     4861 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     4921 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaaggcta gcggatcagg
     4981 tcaatgtact aactacgctt tgttgaagct tgccggcgat gttgaaagta accccggtcc
     5041 tggatccatg tccagactgg acaagagcaa agtcataaac ggcgctctgg aattactcaa
     5101 tggagtcggt atcgaaggcc tgacgacaag gaaactcgct caaaagctgg gagttgagca
     5161 gcctaccctg tactggcacg tgaagaacaa gcgggccctg ctcgatgccc tgccaatcga
     5221 gatgctggac aggcatcata cccacttctg ccccctggaa ggcgagtcat ggcaagactt
     5281 tctgcggaac aacgccaagt cattccgctg tgctctcctc tcacatcgcg acggggctaa
     5341 agtgcatctc ggcacccgcc caacagagaa acagtacgaa accctggaaa atcagctcgc
     5401 gttcctgtgt cagcaaggct tctccctgga gaacgcactg tacgctctgt ccgccgtggg
     5461 ccactttaca ctgggctgcg tattggagga acaggagcat caagtagcaa aagaggaaag
     5521 agagacacct accaccgatt ctatgccccc acttctgaga caagcaattg agctgttcga
     5581 ccggcaggga gccgaacctg ccttcctttt cggcctggaa ctaatcatat gtggcctgga
     5641 gaaacagcta aagtgcgaaa gcggcgggcc ggcctcgagc gaattccagt acctgccaga
     5701 tacagacgat cgtcaccgga ttgaggagaa acgtaaaagg acatatgaga ccttcaagag
     5761 catcatgaag aagagtcctt tcagcggacc caccgacccc cggcctccac ctcgacgcat
     5821 tgctgtgcct tcccgcagct cagcttctgt ccccaagcca gcaccccagc cctatccctt
     5881 tacgtcatcc ctgagcacca tcaactatga tgagtttccc accatggtgt ttccttctgg
     5941 gcagatcagc caggcctcgg ccttggcccc ggcccctccc caagtcctgc cccaggctcc
     6001 agcccctgcc cctgctccag ccatggtatc agctctggcc caggccccag cccctgtccc
     6061 agtcctagcc ccaggccctc ctcaggctgt ggccccacct gcccccaagc ccacccaggc
     6121 tggggaagga acgctgtcag aggccctgct gcagctgcag tttgatgatg aagacctggg
     6181 ggccttgctt ggcaacagca cagacccagc tgtgttcaca gacctggcat ccgtcgacaa
     6241 ctccgagttt cagcagctgc tgaaccaggg catacctgtg gccccccaca caactgagcc
     6301 catgctgatg gagtaccctg aggctataac tcgcctagtg acaggggccc agaggccccc
     6361 cgacccagct cctgctccac tgggggcccc ggggctcccc aatggcctcc tttcaggaga
     6421 tgaagacttc tcctccattg cggacatgga cttctcagcc ctgctgagtc agatcagctc
     6481 ctaagatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg
     6541 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     6601 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     6661 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     6721 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     6781 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     6841 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     6901 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     6961 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     7021 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     7081 cctccccgca tcgataccgt cgacctcgag gttcgcttta agaccaatga cttacaaggc
     7141 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     7201 ccaacgaaga caagatggga tcaattcacc atgggaataa cttcgtatag catacattat
     7261 acgaagttat gctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     7321 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     7381 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     7441 ccttttagtc agtgtggaaa atctctagca gcatctagtt gaatcgccag tgtgtcgagc
     7501 tagcagatct ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc
     7561 tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt
     7621 gtctctcact cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt
     7681 tggtttagag tttggcaaca tatgccatat gctggctgcc atgaacaaag gtggctataa
     7741 agaggtcatc agtatatgaa acagccccct gctgtccatt ccttattcca tagaaaagcc
     7801 ttgacttgag gttagatttt ttttatattt tgttttgtgt tatttttttc tttaacatcc
     7861 ctaaaatttt ccttacatgt tttactagcc agatttttcc tcctctcctg actactccca
     7921 gtcatagctg tccctcttct cttatgaaga tccctcgacc tgcagcccaa gcttggcgta
     7981 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     8041 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     8101 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agcggatccg
     8161 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     8221 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     8281 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     8341 ctaggctttt gcaaaaagct aacttgttta ttgcagctta taatggttac aaataaagca
     8401 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     8461 ccaaactcat caatgtatct tatcatgtct ggatccgctg cattaatgaa tcggccaacg
     8521 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     8581 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     8641 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     8701 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     8761 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     8821 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     8881 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg
     8941 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     9001 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     9061 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     9121 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     9181 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     9241 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     9301 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     9361 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     9421 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     9481 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     9541 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     9601 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     9661 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     9721 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     9781 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     9841 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     9901 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     9961 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
    10021 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
    10081 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
    10141 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
    10201 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
    10261 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
    10321 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
    10381 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
    10441 acaaataggg gttccgcgca catttccccg aaaagtgcca cctg
//
