LOCUS       sequence-215772-       11942 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TET-inducible expression of a constitutively active form of the 
            BetaCatenin protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11942)
  AUTHORS   Boitard M, Bocchi R, Egervari K, Petrenko V, Viale B, Gremaud S, 
            Zgraggen E, Salmon P, Kiss JZ
  TITLE     Wnt signaling regulates multipolar-to-bipolar transition of 
            migrating neurons in the cerebral cortex.
  JOURNAL   Cell Rep. 2015 Mar 3;10(8):1349-61. doi: 
            10.1016/j.celrep.2015.01.061. Epub 2015 Feb 26.
  PUBMED    25732825
REFERENCE   2  (bases 1 to 11942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.01.061"; journalName: "Cell Rep"; date: 
            "2015-03-3- 3"; volume: "10"; issue: "8"; pages: "1349-61"
COMMENT     Sequence Label: pCWXPGR-pTF-betaCatenin
FEATURES             Location/Qualifiers
     source          1..11942
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             385..642
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    689..814
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1306..1423
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    1783..2016
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2201..2245
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     protein_bind    2522..2542
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    2569..2587
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    2610..2628
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    2774..2792
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    2854..2878
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2887..2910
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(5134..5158)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5176..5686
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     5605..5623
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      5702..5711
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5708..5710,5711..5713,5714..6424)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5708..5710,5711..5713,5714..6424)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5753..5774)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6014..6033)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6361..6382
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             6440..6499
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             7568..7939
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     misc_feature    7959..8547
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8012..8032)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8430..8441)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8550..8566)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8551..8567)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    complement(8695..8728)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             8748..8928
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8966..8985)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    9031..9086
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(9085..9104)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(9445..9461)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9445..9461)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9458..9480)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9469..9485
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9493..9499,9500..9517,9518..9523))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9538..9559
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        9618..9813
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9664..9799
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    9819..9953
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9856..9875)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9910..9929
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10021..10038)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10192..10780)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10272..10291)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10951..11742,11743..11811))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11574..11593
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11812..11916)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggaggcg tggcctgggc gggactgggg agtggcgagc
      421 cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc
      481 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
      541 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
      601 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
      661 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
      721 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
      781 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
      841 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
      901 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
      961 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     1021 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     1081 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaaaaa
     1141 agcacagcaa gcagcaggat cttcagacct ggaaattccc tacaatcccc aaagtcaagg
     1201 agtagtagaa tctatgaata aagaattaaa gaaaattata ggacaggtaa gagatcaggc
     1261 tgaacatctt aagacagcag tacaaatggc agtattcatc cacaatttta aaagaaaagg
     1321 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     1381 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     1441 cagcagaaat ccactttgga aaggaccagc aaagctcctc tggaaaggtg aaggggcagt
     1501 agtaatacaa gataatagtg acataaaagt agtgccaaga agaaaagcaa agatcattag
     1561 ggattatgga aaacagatgg caggtgatga ttgtgtggca agtagacagg atgaggatta
     1621 gaacatggaa aagtttagta aaacaccata aggaggagat atgagggaca attggagaag
     1681 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1741 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1801 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1861 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1921 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1981 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     2041 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     2101 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     2161 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     2221 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     2281 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     2341 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     2401 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     2461 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgctagt gattatcgga
     2521 tcaactttgt atagaaaagt tgggtacaac tcccactaga catatcctac tctatcattg
     2581 atagagtgac ccttccatag acatatccta ctctatcatt gatagagtga cccttccact
     2641 aggttcactt ttctctatca ttgataggga gtggtaaaca attgatggac aaaatctatc
     2701 aatgatagag agtgcatgtt actagtattg ttcttctcta tcattgatag tgactttgcc
     2761 taatcactta gcaactctat cattgataga gttctagtat tagcaaggcc tttcctctat
     2821 ataaggaagt tcatttcatt tggagaagat cccccaagtt tgtacaaaaa agcaggcttc
     2881 accatggact acaaagacga tgacgacaag cttgcggccg ctctgagtgg taaaggcaat
     2941 cctgaggaag aggatgtgga tacctcccaa gtcctgtatg agtgggaaca gggattttct
     3001 cagtccttca ctcaagaaca agtagctgat attgatggac agtatgcaat gactcgagct
     3061 cagagggtac gagctgctat gttccctgag acattagatg agggcatgca gatcccatct
     3121 acacagtttg atgctgctca tcccactaat gtccagcgtt tggctgaacc atcacagatg
     3181 ctgaaacatg cagttgtaaa cttgattaac tatcaagatg atgcagaact tgccacacgt
     3241 gcaatccctg aactgacaaa actgctaaat gacgaggacc aggtggtggt taataaggct
     3301 gcagttatgg tccatcagct ttctaaaaag gaagcttcca gacacgctat catgcgttct
     3361 cctcagatgg tgtctgctat tgtacgtacc atgcagaata caaatgatgt agaaacagct
     3421 cgttgtaccg ctgggacctt gcataacctt tcccatcatc gtgagggctt actggccatc
     3481 tttaagtctg gaggcattcc tgccctggtg aaaatgcttg gttcaccagt ggattctgtg
     3541 ttgttttatg ccattacaac tctccacaac cttttattac atcaagaagg agctaaaatg
     3601 gcagtgcgtt tagctggtgg gctgcagaaa atggttgcct tgctcaacaa aacaaatgtt
     3661 aaattcttgg ctattacgac agactgcctt caaattttag cttatggcaa ccaagaaagc
     3721 aagctcatca tactggctag tggtggaccc caagctttag taaatataat gaggacctat
     3781 acttacgaaa aactactgtg gaccacaagc agagtgctga aggtgctatc tgtctgctct
     3841 agtaataagc cggctattgt agaagctggt ggaatgcaag ctttaggact tcacctgaca
     3901 gatccaagtc aacgtcttgt tcagaactgt ctttggactc tcaggaatct ttcagatgct
     3961 gcaactaaac aggaagggat ggaaggtctc cttgggactc ttgttcagct tctgggttca
     4021 gatgatataa atgtggtcac ctgtgcagct ggaattcttt ctaacctcac ttgcaataat
     4081 tataagaaca agatgatggt ctgccaagtg ggtggtatag aggctcttgt gcgtactgtc
     4141 cttcgggctg gtgacaggga agacatcact gagcctgcca tctgtgctct tcgtcatctg
     4201 accagccgac accaagaagc agagatggcc cagaatgcag ttcgccttca ctatggacta
     4261 ccagttgtgg ttaagctctt acacccacca tcccactggc ctctgataaa ggctactgtt
     4321 ggattgattc gaaatcttgc cctttgtccc gcaaatcatg cacctttgcg tgagcagggt
     4381 gccattccac gactagttca gttgcttgtt cgtgcacatc aggataccca gcgccgtacg
     4441 tccatgggtg ggacacagca gcaatttgtg gagggggtcc gcatggaaga aatagttgaa
     4501 ggttgtaccg gagcccttca catcctagct cgggatgttc acaaccgaat tgttatcaga
     4561 ggactaaata ccattccatt gtttgtgcag ctgctttatt ctcccattga aaacatccaa
     4621 agagtagctg caggggtcct ctgtgaactt gctcaggaca aggaagctgc agaagctatt
     4681 gaagctgagg gagccacagc tcctctgaca gagttacttc actctaggaa tgaaggtgtg
     4741 gcgacatatg cagctgctgt tttgttccga atgtctgagg acaagccaca agattacaag
     4801 aaacggcttt cagttgagct gaccagctct ctcttcagaa cagagccaat ggcttggaat
     4861 gagactgctg atcttggact tgatattggt gcccagggag aaccccttgg atatcgccag
     4921 gatgatccta gctatcgttc ttttcactct ggtggatatg gccaggatgc cttgggtatg
     4981 gaccccatga tggaacatga gatgggtggc caccaccctg gtgctgacta tccagttgat
     5041 gggctgccag atctggggca tgcccaggac ctcatggatg ggctgcctcc aggtgacagc
     5101 aatcagctgg cctggtttga tactgacctg taaacccagc tttcttgtac aaagtggtga
     5161 taatcgaatt cccacggggt tggggttgcg ccttttccaa ggcagccctg ggtttgcgca
     5221 gggacgcggc tgctctgggc gtggttccgg gaaacgcagc ggcgccgacc ctgggtctcg
     5281 cacattcttc acgtccgttc gcagcgtcac ccggatcttc gccgctaccc ttgtgggccc
     5341 cccggcgacg cttcctgctc cgcccctaag tcgggaaggt tccttgcggt tcgcggcgtg
     5401 ccggacgtga caaacggaag ccgcacgtct cactagtacc ctcgcagacg gacagcgcca
     5461 gggagcaatg gcagcgcgcc gaccgcgatg ggctgtggcc aatagcggct gctcagcggg
     5521 gcgcgccgag agcagcggcc gggaaggggc ggtgcgggag gcggggtgtg gggcggtagt
     5581 gtgggccctg ttcctgcccg cgcggtgttc cgcattctgc aagcctccgg agcgcacgtc
     5641 ggcagtcggc tccctcgttg accgaatcac cgacctctct ccccaggggg atccaccggt
     5701 cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     5761 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     5821 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     5881 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     5941 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     6001 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     6061 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     6121 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     6181 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     6241 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     6301 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     6361 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     6421 caaggctagc ggatcaggtc aatgtactaa ctacgctttg ttgaagcttg ccggcgatgt
     6481 tgaaagtaac cccggtcctg gatccatgtc cagactggac aagagcaaag tcataaacgg
     6541 cgctctggaa ttactcaatg gagtcggtat cgaaggcctg acgacaagga aactcgctca
     6601 aaagctggga gttgagcagc ctaccctgta ctggcacgtg aagaacaagc gggccctgct
     6661 cgatgccctg ccaatcgaga tgctggacag gcatcatacc cacttctgcc ccctggaagg
     6721 cgagtcatgg caagactttc tgcggaacaa cgccaagtca ttccgctgtg ctctcctctc
     6781 acatcgcgac ggggctaaag tgcatctcgg cacccgccca acagagaaac agtacgaaac
     6841 cctggaaaat cagctcgcgt tcctgtgtca gcaaggcttc tccctggaga acgcactgta
     6901 cgctctgtcc gccgtgggcc actttacact gggctgcgta ttggaggaac aggagcatca
     6961 agtagcaaaa gaggaaagag agacacctac caccgattct atgcccccac ttctgagaca
     7021 agcaattgag ctgttcgacc ggcagggagc cgaacctgcc ttccttttcg gcctggaact
     7081 aatcatatgt ggcctggaga aacagctaaa gtgcgaaagc ggcgggccgg cctcgagcga
     7141 attccagtac ctgccagata cagacgatcg tcaccggatt gaggagaaac gtaaaaggac
     7201 atatgagacc ttcaagagca tcatgaagaa gagtcctttc agcggaccca ccgacccccg
     7261 gcctccacct cgacgcattg ctgtgccttc ccgcagctca gcttctgtcc ccaagccagc
     7321 accccagccc tatcccttta cgtcatccct gagcaccatc aactatgatg agtttcccac
     7381 catggtgttt ccttctgggc agatcagcca ggcctcggcc ttggccccgg cccctcccca
     7441 agtcctgccc caggctccag cccctgcccc tgctccagcc atggtatcag ctctggccca
     7501 ggccccagcc cctgtcccag tcctagcccc aggccctcct caggctgtgg ccccacctgc
     7561 ccccaagccc acccaggctg gggaaggaac gctgtcagag gccctgctgc agctgcagtt
     7621 tgatgatgaa gacctggggg ccttgcttgg caacagcaca gacccagctg tgttcacaga
     7681 cctggcatcc gtcgacaact ccgagtttca gcagctgctg aaccagggca tacctgtggc
     7741 cccccacaca actgagccca tgctgatgga gtaccctgag gctataactc gcctagtgac
     7801 aggggcccag aggccccccg acccagctcc tgctccactg ggggccccgg ggctccccaa
     7861 tggcctcctt tcaggagatg aagacttctc ctccattgcg gacatggact tctcagccct
     7921 gctgagtcag atcagctcct aagatcaagc ttatcgataa tcaacctctg gattacaaaa
     7981 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     8041 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     8101 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     8161 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     8221 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     8281 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     8341 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     8401 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     8461 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     8521 ggatctccct ttgggccgcc tccccgcatc gataccgtcg acctcgaggt tcgctttaag
     8581 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     8641 ggaagggcta attcactccc aacgaagaca agatgggatc aattcaccat gggaataact
     8701 tcgtatagca tacattatac gaagttatgc tgctttttgc ttgtactggg tctctctggt
     8761 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     8821 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     8881 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagc atctagttga
     8941 atcgccagtg tgtcgagcta gcagatcttt ttccctctgc caaaaattat ggggacatca
     9001 tgaagcccct tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag
     9061 tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa
     9121 aacatcagaa tgagtatttg gtttagagtt tggcaacata tgccatatgc tggctgccat
     9181 gaacaaaggt ggctataaag aggtcatcag tatatgaaac agccccctgc tgtccattcc
     9241 ttattccata gaaaagcctt gacttgaggt tagatttttt ttatattttg ttttgtgtta
     9301 tttttttctt taacatccct aaaattttcc ttacatgttt tactagccag atttttcctc
     9361 ctctcctgac tactcccagt catagctgtc cctcttctct tatgaagatc cctcgacctg
     9421 cagcccaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     9481 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     9541 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     9601 gtcgtgccag cggatccgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     9661 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     9721 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     9781 ggaggctttt ttggaggcct aggcttttgc aaaaagctaa cttgtttatt gcagcttata
     9841 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     9901 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atccgctgca
     9961 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
    10021 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
    10081 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
    10141 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
    10201 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
    10261 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
    10321 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
    10381 ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
    10441 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
    10501 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
    10561 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
    10621 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
    10681 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
    10741 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
    10801 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
    10861 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
    10921 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
    10981 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
    11041 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
    11101 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
    11161 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
    11221 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
    11281 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
    11341 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
    11401 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
    11461 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
    11521 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
    11581 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
    11641 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
    11701 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
    11761 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
    11821 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
    11881 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
    11941 tg
//
