LOCUS       sequence-215775-       10976 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TET-inducible expression of the Kir2.1 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10976)
  TITLE     Salmon Lab plasmids submitted
REFERENCE   2  (bases 1 to 10976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCWXPGR-pTF-Kir2.1
FEATURES             Location/Qualifiers
     source          1..10976
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             385..642
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    689..814
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1306..1423
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    1783..2016
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2201..2245
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     protein_bind    2522..2542
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    2569..2587
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    2610..2628
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    2774..2792
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    2854..2878
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2947..2956
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(4168..4192)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4210..4720
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4639..4657
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      4736..4745
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4742..4744,4745..4747,4748..5458)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4742..4744,4745..4747,4748..5458)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4787..4808)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5048..5067)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5395..5416
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5474..5533
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             6602..6973
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     misc_feature    6993..7581
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7046..7066)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7464..7475)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7584..7600)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7585..7601)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    complement(7729..7762)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             7782..7962
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8000..8019)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    8065..8120
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8119..8138)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(8479..8495)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8479..8495)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8492..8514)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8503..8519
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8527..8533,8534..8551,8552..8557))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8572..8593
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8652..8847
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8698..8833
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    8853..8987
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8890..8909)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8944..8963
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9055..9072)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9226..9814)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9306..9325)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9985..10776,10777..10845))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10608..10627
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10846..10950)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggaggcg tggcctgggc gggactgggg agtggcgagc
      421 cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc
      481 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
      541 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
      601 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
      661 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
      721 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
      781 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
      841 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
      901 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
      961 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     1021 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     1081 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaaaaa
     1141 agcacagcaa gcagcaggat cttcagacct ggaaattccc tacaatcccc aaagtcaagg
     1201 agtagtagaa tctatgaata aagaattaaa gaaaattata ggacaggtaa gagatcaggc
     1261 tgaacatctt aagacagcag tacaaatggc agtattcatc cacaatttta aaagaaaagg
     1321 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     1381 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     1441 cagcagaaat ccactttgga aaggaccagc aaagctcctc tggaaaggtg aaggggcagt
     1501 agtaatacaa gataatagtg acataaaagt agtgccaaga agaaaagcaa agatcattag
     1561 ggattatgga aaacagatgg caggtgatga ttgtgtggca agtagacagg atgaggatta
     1621 gaacatggaa aagtttagta aaacaccata aggaggagat atgagggaca attggagaag
     1681 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1741 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1801 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1861 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1921 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1981 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     2041 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     2101 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     2161 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     2221 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     2281 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     2341 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     2401 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     2461 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgctagt gattatcgga
     2521 tcaactttgt atagaaaagt tgggtacaac tcccactaga catatcctac tctatcattg
     2581 atagagtgac ccttccatag acatatccta ctctatcatt gatagagtga cccttccact
     2641 aggttcactt ttctctatca ttgataggga gtggtaaaca attgatggac aaaatctatc
     2701 aatgatagag agtgcatgtt actagtattg ttcttctcta tcattgatag tgactttgcc
     2761 taatcactta gcaactctat cattgataga gttctagtat tagcaaggcc tttcctctat
     2821 ataaggaagt tcatttcatt tggagaagat cccccaagtt tgtacaaaaa agcaggcttc
     2881 accatgggca gtgtgagaac caaccgctac agcatcgtct cttcagaaga agacggtatg
     2941 aagttggcca ccatggcagt tgcaaatggc tttgggaacg ggaagagtaa agtccacacc
     3001 cgacaacagt gcaggagccg ctttgtgaag aaagatggcc actgtaatgt tcagttcatc
     3061 aatgtgggtg agaaggggca acggtacctc gcagacatct tcaccacgtg tgtggacatt
     3121 cgctggcggt ggatgctggt tatcttctgc ctggctttcg tcctgtcatg gctgtttttt
     3181 ggctgtgtgt tttggttgat agctctgctc catggggacc tggatgcatc caaagagggc
     3241 aaagcttgtg tgtccgaggt caacagcttc acggctgcct tcctcttctc cattgagacc
     3301 cagacaacca taggctatgg tttcagatgt gtcacggatg aatgcccaat tgctgttttc
     3361 atggtggtgt tccagtcaat cgtgggctgc atcatcgatg ctttcatcat tggcgcagtc
     3421 atggccaaga tggcaaagcc aaagaagaga aacgagactc ttgtcttcag tcacaatgcc
     3481 gtgattgcca tgagagacgg caagctgtgt ttgatgtggc gagtgggcaa tcttcggaaa
     3541 agccacttgg tggaagctca tgttcgagca cagctcctca aatccagaat tacttctgaa
     3601 ggggagtata tccctctgga tcaaatagac atcaatgttg ggtttgacag tggaatcgat
     3661 cgtatatttc tggtgtcccc aatcactata gtccatgaaa tagatgaaga cagtccttta
     3721 tatgatttga gtaaacagga cattgacaac gcagactttg aaatcgtggt catactggaa
     3781 ggcatggtgg aagccactgc catgacgaca cagtgccgta gctcttatct agcaaatgaa
     3841 atcctgtggg gccaccgcta tgagcctgtg ctctttgaag agaagcacta ctacaaagtg
     3901 gactactcca ggttccacaa aacttacgaa gtccccaaca ctcccctttg tagtgccaga
     3961 gacttagcag aaaagaaata tatcctctca aatgcaaatt cattttgcta tgaaaatgaa
     4021 gttgccctca caagcaaaga ggaagacgac agtgaaaatg gagttccaga aagcactagt
     4081 acggacacgc cccctgacat agaccttcac aaccaggcaa gtgtacctct agagcccagg
     4141 cccttacggc gagagtcgga gatatgaacc cagctttctt gtacaaagtg gtgataatcg
     4201 aattcccacg gggttggggt tgcgcctttt ccaaggcagc cctgggtttg cgcagggacg
     4261 cggctgctct gggcgtggtt ccgggaaacg cagcggcgcc gaccctgggt ctcgcacatt
     4321 cttcacgtcc gttcgcagcg tcacccggat cttcgccgct acccttgtgg gccccccggc
     4381 gacgcttcct gctccgcccc taagtcggga aggttccttg cggttcgcgg cgtgccggac
     4441 gtgacaaacg gaagccgcac gtctcactag taccctcgca gacggacagc gccagggagc
     4501 aatggcagcg cgccgaccgc gatgggctgt ggccaatagc ggctgctcag cggggcgcgc
     4561 cgagagcagc ggccgggaag gggcggtgcg ggaggcgggg tgtggggcgg tagtgtgggc
     4621 cctgttcctg cccgcgcggt gttccgcatt ctgcaagcct ccggagcgca cgtcggcagt
     4681 cggctccctc gttgaccgaa tcaccgacct ctctccccag ggggatccac cggtcgccac
     4741 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     4801 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     4861 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     4921 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
     4981 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     5041 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     5101 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     5161 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
     5221 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     5281 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     5341 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
     5401 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaaggc
     5461 tagcggatca ggtcaatgta ctaactacgc tttgttgaag cttgccggcg atgttgaaag
     5521 taaccccggt cctggatcca tgtccagact ggacaagagc aaagtcataa acggcgctct
     5581 ggaattactc aatggagtcg gtatcgaagg cctgacgaca aggaaactcg ctcaaaagct
     5641 gggagttgag cagcctaccc tgtactggca cgtgaagaac aagcgggccc tgctcgatgc
     5701 cctgccaatc gagatgctgg acaggcatca tacccacttc tgccccctgg aaggcgagtc
     5761 atggcaagac tttctgcgga acaacgccaa gtcattccgc tgtgctctcc tctcacatcg
     5821 cgacggggct aaagtgcatc tcggcacccg cccaacagag aaacagtacg aaaccctgga
     5881 aaatcagctc gcgttcctgt gtcagcaagg cttctccctg gagaacgcac tgtacgctct
     5941 gtccgccgtg ggccacttta cactgggctg cgtattggag gaacaggagc atcaagtagc
     6001 aaaagaggaa agagagacac ctaccaccga ttctatgccc ccacttctga gacaagcaat
     6061 tgagctgttc gaccggcagg gagccgaacc tgccttcctt ttcggcctgg aactaatcat
     6121 atgtggcctg gagaaacagc taaagtgcga aagcggcggg ccggcctcga gcgaattcca
     6181 gtacctgcca gatacagacg atcgtcaccg gattgaggag aaacgtaaaa ggacatatga
     6241 gaccttcaag agcatcatga agaagagtcc tttcagcgga cccaccgacc cccggcctcc
     6301 acctcgacgc attgctgtgc cttcccgcag ctcagcttct gtccccaagc cagcacccca
     6361 gccctatccc tttacgtcat ccctgagcac catcaactat gatgagtttc ccaccatggt
     6421 gtttccttct gggcagatca gccaggcctc ggccttggcc ccggcccctc cccaagtcct
     6481 gccccaggct ccagcccctg cccctgctcc agccatggta tcagctctgg cccaggcccc
     6541 agcccctgtc ccagtcctag ccccaggccc tcctcaggct gtggccccac ctgcccccaa
     6601 gcccacccag gctggggaag gaacgctgtc agaggccctg ctgcagctgc agtttgatga
     6661 tgaagacctg ggggccttgc ttggcaacag cacagaccca gctgtgttca cagacctggc
     6721 atccgtcgac aactccgagt ttcagcagct gctgaaccag ggcatacctg tggcccccca
     6781 cacaactgag cccatgctga tggagtaccc tgaggctata actcgcctag tgacaggggc
     6841 ccagaggccc cccgacccag ctcctgctcc actgggggcc ccggggctcc ccaatggcct
     6901 cctttcagga gatgaagact tctcctccat tgcggacatg gacttctcag ccctgctgag
     6961 tcagatcagc tcctaagatc aagcttatcg ataatcaacc tctggattac aaaatttgtg
     7021 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     7081 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     7141 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     7201 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     7261 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     7321 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     7381 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     7441 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     7501 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     7561 ccctttgggc cgcctccccg catcgatacc gtcgacctcg aggttcgctt taagaccaat
     7621 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     7681 gctaattcac tcccaacgaa gacaagatgg gatcaattca ccatgggaat aacttcgtat
     7741 agcatacatt atacgaagtt atgctgcttt ttgcttgtac tgggtctctc tggttagacc
     7801 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     7861 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     7921 gatccctcag acccttttag tcagtgtgga aaatctctag cagcatctag ttgaatcgcc
     7981 agtgtgtcga gctagcagat ctttttccct ctgccaaaaa ttatggggac atcatgaagc
     8041 cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca atagtgtgtt
     8101 ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat ttaaaacatc
     8161 agaatgagta tttggtttag agtttggcaa catatgccat atgctggctg ccatgaacaa
     8221 aggtggctat aaagaggtca tcagtatatg aaacagcccc ctgctgtcca ttccttattc
     8281 catagaaaag ccttgacttg aggttagatt ttttttatat tttgttttgt gttatttttt
     8341 tctttaacat ccctaaaatt ttccttacat gttttactag ccagattttt cctcctctcc
     8401 tgactactcc cagtcatagc tgtccctctt ctcttatgaa gatccctcga cctgcagccc
     8461 aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     8521 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     8581 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     8641 ccagcggatc cgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     8701 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     8761 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     8821 ttttttggag gcctaggctt ttgcaaaaag ctaacttgtt tattgcagct tataatggtt
     8881 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     8941 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatccgc tgcattaatg
     9001 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     9061 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     9121 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     9181 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     9241 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     9301 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     9361 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     9421 atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     9481 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     9541 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     9601 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     9661 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     9721 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     9781 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     9841 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     9901 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     9961 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
    10021 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
    10081 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
    10141 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
    10201 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
    10261 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
    10321 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
    10381 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
    10441 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
    10501 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
    10561 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
    10621 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
    10681 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
    10741 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
    10801 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
    10861 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
    10921 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctg
//
