LOCUS       sequence-215776-        9850 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TET-inducible expression of Erythropoietin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9850)
  TITLE     Salmon Lab plasmids submitted
REFERENCE   2  (bases 1 to 9850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCLXEBR-pTF-Epo
FEATURES             Location/Qualifiers
     source          1..9850
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             385..642
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    689..814
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1306..1423
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    1783..2016
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2201..2245
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     protein_bind    2536..2554
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    2577..2595
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    2741..2759
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    2844..2868
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3453..3477)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     3514..3530
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     3515..3531
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    3546..3663
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3719..3930
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             3949..4344
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             4354..4413
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             5476..5847
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     misc_feature    5873..6461
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5926..5946)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6344..6355)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(6603..6636)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             6656..6836
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6874..6893)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6939..6994
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6993..7012)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7353..7369)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7353..7369)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7366..7388)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7377..7393
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7401..7407,7408..7425,7426..7431))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7446..7467
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7526..7721
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7572..7707
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7727..7861
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7764..7783)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7818..7837
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7929..7946)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8100..8688)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8180..8199)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8859..9650,9651..9719))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9482..9501
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9720..9824)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggaggcg tggcctgggc gggactgggg agtggcgagc
      421 cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc
      481 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
      541 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
      601 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
      661 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
      721 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
      781 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
      841 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
      901 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
      961 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     1021 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     1081 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaaaaa
     1141 agcacagcaa gcagcaggat cttcagacct ggaaattccc tacaatcccc aaagtcaagg
     1201 agtagtagaa tctatgaata aagaattaaa gaaaattata ggacaggtaa gagatcaggc
     1261 tgaacatctt aagacagcag tacaaatggc agtattcatc cacaatttta aaagaaaagg
     1321 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     1381 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     1441 cagcagaaat ccactttgga aaggaccagc aaagctcctc tggaaaggtg aaggggcagt
     1501 agtaatacaa gataatagtg acataaaagt agtgccaaga agaaaagcaa agatcattag
     1561 ggattatgga aaacagatgg caggtgatga ttgtgtggca agtagacagg atgaggatta
     1621 gaacatggaa aagtttagta aaacaccata aggaggagat atgagggaca attggagaag
     1681 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1741 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1801 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1861 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1921 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1981 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     2041 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     2101 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     2161 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     2221 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     2281 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     2341 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     2401 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     2461 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttcg gtacaactcc
     2521 cactagacat atcctactct atcattgata gagtgaccct tccatagaca tatcctactc
     2581 tatcattgat agagtgaccc ttccactagg ttcacttttc tctatcattg atagggagtg
     2641 gtaaacaatt gatggacaaa atctatcaat gatagagagt gcatgttact agtattgttc
     2701 ttctctatca ttgatagtga ctttgcctaa tcacttagca actctatcat tgatagagtt
     2761 ctagtattag caaggccttt cctctatata aggaagttca tttcatttgg agaagatccc
     2821 cggggatcct ctagagtcga atcacaagtt tgtacaaaaa agcaggcttc accatggggg
     2881 tgcccgaacg tcccaccctg ctgcttttac tatccttgct actgattcct ctgggcctcc
     2941 cagtcctctg cgctccccca cgcctcattt gcgacagtcg cgttctggag aggtacatct
     3001 tggaggccaa ggaggcagaa aatgtcacaa tgggctgtgc agaaggtccc agactgagtg
     3061 agaatattac cgtcccagat accaaagtca acttctacgc ttggaaaaga atgaaggtgg
     3121 aagaacaggc tgtagaagtt tggcaaggcc tgtctctgct ctcagaagcc atcctgcagg
     3181 cccaggctct gcaggccaat tcctcccagc caccagagag tcttcagctt catatagaca
     3241 aagccatcag tgggctacgt agcctcactt cactgcttcg ggtgctggga gctcagaagg
     3301 aattgatgtc gcctccagac gccacccaag ccgctccact ccgaacactc acagcggata
     3361 ctttctgcaa gctcttccgg gtctactcca acttcctccg ggggaaactg aagctgtaca
     3421 cgggggaggc ctgcaggaga ggggacaggt gaacccagct ttcttgtaca aagtggtgat
     3481 tcgacctgca ggcatgcaag ctcgggcccc ccctcgaggt cgacggtatc gataagcttg
     3541 ataactttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
     3601 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
     3661 tttccgatca cgagactagc ctcgagaagc ttgatcgatg gctccggtgc ccgtcagtgg
     3721 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3781 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3841 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3901 tttcgcaacg ggtttgccgc cagaacacag gtgtcgtgac gcggatccat ggccaagcct
     3961 ttgtctcaag aagaatccac cctcattgaa agagcaacgg ctacaatcaa cagcatcccc
     4021 atctctgaag actacagcgt cgccagcgca gctctctcta gcgacggccg catcttcact
     4081 ggtgtcaatg tatatcattt tactggggga ccttgtgcag aactcgtggt gctgggcact
     4141 gctgctgctg cggcagctgg caacctgact tgtatcgtcg cgatcggaaa tgagaacagg
     4201 ggcatcttga gcccctgcgg acggtgccga caggtgcttc tcgatctgca tcctgggatc
     4261 aaagccatag tgaaggacag tgatggacag ccgacggcag ttgggattcg tgaattgctg
     4321 ccctctggtt atgtgtggga gggcggatca ggtcaatgta ctaactacgc tttgttgaag
     4381 cttgccggcg atgttgaaag taaccccggt cctatgtcca gactggacaa gagcaaagtc
     4441 ataaacggcg ctctggaatt actcaatgga gtcggtatcg aaggcctgac gacaaggaaa
     4501 ctcgctcaaa agctgggagt tgagcagcct accctgtact ggcacgtgaa gaacaagcgg
     4561 gccctgctcg atgccctgcc aatcgagatg ctggacaggc atcataccca cttctgcccc
     4621 ctggaaggcg agtcatggca agactttctg cggaacaacg ccaagtcatt ccgctgtgct
     4681 ctcctctcac atcgcgacgg ggctaaagtg catctcggca cccgcccaac agagaaacag
     4741 tacgaaaccc tggaaaatca gctcgcgttc ctgtgtcagc aaggcttctc cctggagaac
     4801 gcactgtacg ctctgtccgc cgtgggccac tttacactgg gctgcgtatt ggaggaacag
     4861 gagcatcaag tagcaaaaga ggaaagagag acacctacca ccgattctat gcccccactt
     4921 ctgagacaag caattgagct gttcgaccgg cagggagccg aacctgcctt ccttttcggc
     4981 ctggaactaa tcatatgtgg cctggagaaa cagctaaagt gcgaaagcgg cgggccggcc
     5041 tcgagcgaat tccagtacct gccagataca gacgatcgtc accggattga ggagaaacgt
     5101 aaaaggacat atgagacctt caagagcatc atgaagaaga gtcctttcag cggacccacc
     5161 gacccccggc ctccacctcg acgcattgct gtgccttccc gcagctcagc ttctgtcccc
     5221 aagccagcac cccagcccta tccctttacg tcatccctga gcaccatcaa ctatgatgag
     5281 tttcccacca tggtgtttcc ttctgggcag atcagccagg cctcggcctt ggccccggcc
     5341 cctccccaag tcctgcccca ggctccagcc cctgcccctg ctccagccat ggtatcagct
     5401 ctggcccagg ccccagcccc tgtcccagtc ctagccccag gccctcctca ggctgtggcc
     5461 ccacctgccc ccaagcccac ccaggctggg gaaggaacgc tgtcagaggc cctgctgcag
     5521 ctgcagtttg atgatgaaga cctgggggcc ttgcttggca acagcacaga cccagctgtg
     5581 ttcacagacc tggcatccgt cgacaactcc gagtttcagc agctgctgaa ccagggcata
     5641 cctgtggccc cccacacaac tgagcccatg ctgatggagt accctgaggc tataactcgc
     5701 ctagtgacag gggcccagag gccccccgac ccagctcctg ctccactggg ggccccgggg
     5761 ctccccaatg gcctcctttc aggagatgaa gacttctcct ccattgcgga catggacttc
     5821 tcagccctgc tgagtcagat cagctcctaa agatccgcgt cgtacggtcg acaatcaacc
     5881 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     5941 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     6001 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     6061 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     6121 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     6181 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     6241 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt
     6301 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     6361 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     6421 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaattc gagctcggta
     6481 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     6541 gggggactgg aagggctaat tcactcccaa cgaagacaag atgggatcaa ttcaccatgg
     6601 gaataacttc gtatagcata cattatacga agttatgctg ctttttgctt gtactgggtc
     6661 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     6721 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     6781 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagcat
     6841 ctagttgaat cgccagtgtg tcgagctagc agatcttttt ccctctgcca aaaattatgg
     6901 ggacatcatg aagccccttg agcatctgac ttctggctaa taaaggaaat ttattttcat
     6961 tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa
     7021 tcatttaaaa catcagaatg agtatttggt ttagagtttg gcaacatatg ccatatgctg
     7081 gctgccatga acaaaggtgg ctataaagag gtcatcagta tatgaaacag ccccctgctg
     7141 tccattcctt attccataga aaagccttga cttgaggtta gatttttttt atattttgtt
     7201 ttgtgttatt tttttcttta acatccctaa aattttcctt acatgtttta ctagccagat
     7261 ttttcctcct ctcctgacta ctcccagtca tagctgtccc tcttctctta tgaagatccc
     7321 tcgacctgca gcccaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     7381 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     7441 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     7501 ggaaacctgt cgtgccagcg gatccgcatc tcaattagtc agcaaccata gtcccgcccc
     7561 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     7621 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     7681 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctaact tgtttattgc
     7741 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     7801 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
     7861 ccgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     7921 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     7981 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     8041 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     8101 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     8161 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     8221 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     8281 gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     8341 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     8401 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     8461 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     8521 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     8581 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     8641 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     8701 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     8761 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     8821 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     8881 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     8941 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     9001 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     9061 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     9121 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     9181 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     9241 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     9301 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     9361 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     9421 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     9481 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     9541 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     9601 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     9661 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     9721 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     9781 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     9841 gtgccacctg
//
