LOCUS       sequence-215777-       10508 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TET-inducible expression of Erythropoietin receptor without 
            cytoplasmic tail.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10508)
  TITLE     Salmon Lab plasmids submitted
REFERENCE   2  (bases 1 to 10508)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCWXPGR-pTF-EpoRtm
FEATURES             Location/Qualifiers
     source          1..10508
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             385..642
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    689..814
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1306..1423
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    1783..2016
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2201..2245
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     protein_bind    2522..2542
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    2569..2587
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    2610..2628
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    2774..2792
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    2854..2878
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3700..3724)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        3742..4252
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4171..4189
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      4268..4277
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4274..4276,4277..4279,4280..4990)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4274..4276,4277..4279,4280..4990)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4319..4340)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4580..4599)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4927..4948
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5006..5065
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             6134..6505
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     misc_feature    6525..7113
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6578..6598)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6996..7007)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7116..7132)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7117..7133)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    complement(7261..7294)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             7314..7494
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7532..7551)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7597..7652
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7651..7670)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(8011..8027)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8011..8027)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8024..8046)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8035..8051
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8059..8065,8066..8083,8084..8089))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8104..8125
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8184..8379
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8230..8365
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    8385..8519
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8422..8441)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8476..8495
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8587..8604)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8758..9346)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8838..8857)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9517..10308,10309..10377))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10140..10159
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10378..10482)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggaggcg tggcctgggc gggactgggg agtggcgagc
      421 cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc
      481 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
      541 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
      601 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
      661 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
      721 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
      781 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
      841 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
      901 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
      961 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     1021 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     1081 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaaaaa
     1141 agcacagcaa gcagcaggat cttcagacct ggaaattccc tacaatcccc aaagtcaagg
     1201 agtagtagaa tctatgaata aagaattaaa gaaaattata ggacaggtaa gagatcaggc
     1261 tgaacatctt aagacagcag tacaaatggc agtattcatc cacaatttta aaagaaaagg
     1321 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     1381 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     1441 cagcagaaat ccactttgga aaggaccagc aaagctcctc tggaaaggtg aaggggcagt
     1501 agtaatacaa gataatagtg acataaaagt agtgccaaga agaaaagcaa agatcattag
     1561 ggattatgga aaacagatgg caggtgatga ttgtgtggca agtagacagg atgaggatta
     1621 gaacatggaa aagtttagta aaacaccata aggaggagat atgagggaca attggagaag
     1681 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1741 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1801 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1861 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1921 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1981 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     2041 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     2101 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     2161 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     2221 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     2281 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     2341 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     2401 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     2461 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgctagt gattatcgga
     2521 tcaactttgt atagaaaagt tgggtacaac tcccactaga catatcctac tctatcattg
     2581 atagagtgac ccttccatag acatatccta ctctatcatt gatagagtga cccttccact
     2641 aggttcactt ttctctatca ttgataggga gtggtaaaca attgatggac aaaatctatc
     2701 aatgatagag agtgcatgtt actagtattg ttcttctcta tcattgatag tgactttgcc
     2761 taatcactta gcaactctat cattgataga gttctagtat tagcaaggcc tttcctctat
     2821 ataaggaagt tcatttcatt tggagaagat cccccaagtt tgtacaaaaa agcaggcttc
     2881 accatggacc aactcagggt ggcccgctgg cctcgggtta gccccctgtg tctcctactt
     2941 gctggggcag cctgggcatc ctcacccagc ctcccggacc ccaagtttga gagcaaagcc
     3001 gccctgctgg catcccgggg ctccgaagaa cttctatgct tcacccagcg cttggaagac
     3061 ttggtgtgtt tctgggagga agcggcgaac tccgggatgg gcttcaacta cagcttctct
     3121 taccagctcg aaggtgaatc aagaaagtca tgtcgcctgc accaggcgcc caccgtccgc
     3181 ggctccatgc gtttctggtg ttcactgccg accgcggaca cgtcgagttt tgtgccactg
     3241 gagctgcagg tgaccgaggc ctccggatct cctcgctacc accgcatcat ccatatcaat
     3301 gaagtagtgc tcctggacgc ccccgcgggg ctgctggcgc gccgagcaga agagggcagc
     3361 cacgtggtgc tgcggtggct gccacctccc ggggctccta tgaccaccca catccgctac
     3421 gaggtggacg tgtcagcagg caaccgggct ggggggacac aaagggtgga ggtcctggaa
     3481 ggccgcactg agtgtgtcct gagcaacctg cggggcggga cgcgctacac cttcgctgtt
     3541 cgagcacgca tggccgagcc gagcttcagc ggattctgga gcgcctggtc tgagcccgcg
     3601 tcgctactga ctgctagcga cttggaccct ctcatcttga cgctgtctct cattctcgtc
     3661 ctcatctcac tgttgctgac tgtgctggcc ctgctgtaga cccagctttc ttgtacaaag
     3721 tggtgataat cgaattccca cggggttggg gttgcgcctt ttccaaggca gccctgggtt
     3781 tgcgcaggga cgcggctgct ctgggcgtgg ttccgggaaa cgcagcggcg ccgaccctgg
     3841 gtctcgcaca ttcttcacgt ccgttcgcag cgtcacccgg atcttcgccg ctacccttgt
     3901 gggccccccg gcgacgcttc ctgctccgcc cctaagtcgg gaaggttcct tgcggttcgc
     3961 ggcgtgccgg acgtgacaaa cggaagccgc acgtctcact agtaccctcg cagacggaca
     4021 gcgccaggga gcaatggcag cgcgccgacc gcgatgggct gtggccaata gcggctgctc
     4081 agcggggcgc gccgagagca gcggccggga aggggcggtg cgggaggcgg ggtgtggggc
     4141 ggtagtgtgg gccctgttcc tgcccgcgcg gtgttccgca ttctgcaagc ctccggagcg
     4201 cacgtcggca gtcggctccc tcgttgaccg aatcaccgac ctctctcccc agggggatcc
     4261 accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     4321 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
     4381 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     4441 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
     4501 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     4561 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     4621 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     4681 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
     4741 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
     4801 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     4861 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
     4921 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     4981 gctgtacaag gctagcggat caggtcaatg tactaactac gctttgttga agcttgccgg
     5041 cgatgttgaa agtaaccccg gtcctggatc catgtccaga ctggacaaga gcaaagtcat
     5101 aaacggcgct ctggaattac tcaatggagt cggtatcgaa ggcctgacga caaggaaact
     5161 cgctcaaaag ctgggagttg agcagcctac cctgtactgg cacgtgaaga acaagcgggc
     5221 cctgctcgat gccctgccaa tcgagatgct ggacaggcat catacccact tctgccccct
     5281 ggaaggcgag tcatggcaag actttctgcg gaacaacgcc aagtcattcc gctgtgctct
     5341 cctctcacat cgcgacgggg ctaaagtgca tctcggcacc cgcccaacag agaaacagta
     5401 cgaaaccctg gaaaatcagc tcgcgttcct gtgtcagcaa ggcttctccc tggagaacgc
     5461 actgtacgct ctgtccgccg tgggccactt tacactgggc tgcgtattgg aggaacagga
     5521 gcatcaagta gcaaaagagg aaagagagac acctaccacc gattctatgc ccccacttct
     5581 gagacaagca attgagctgt tcgaccggca gggagccgaa cctgccttcc ttttcggcct
     5641 ggaactaatc atatgtggcc tggagaaaca gctaaagtgc gaaagcggcg ggccggcctc
     5701 gagcgaattc cagtacctgc cagatacaga cgatcgtcac cggattgagg agaaacgtaa
     5761 aaggacatat gagaccttca agagcatcat gaagaagagt cctttcagcg gacccaccga
     5821 cccccggcct ccacctcgac gcattgctgt gccttcccgc agctcagctt ctgtccccaa
     5881 gccagcaccc cagccctatc cctttacgtc atccctgagc accatcaact atgatgagtt
     5941 tcccaccatg gtgtttcctt ctgggcagat cagccaggcc tcggccttgg ccccggcccc
     6001 tccccaagtc ctgccccagg ctccagcccc tgcccctgct ccagccatgg tatcagctct
     6061 ggcccaggcc ccagcccctg tcccagtcct agccccaggc cctcctcagg ctgtggcccc
     6121 acctgccccc aagcccaccc aggctgggga aggaacgctg tcagaggccc tgctgcagct
     6181 gcagtttgat gatgaagacc tgggggcctt gcttggcaac agcacagacc cagctgtgtt
     6241 cacagacctg gcatccgtcg acaactccga gtttcagcag ctgctgaacc agggcatacc
     6301 tgtggccccc cacacaactg agcccatgct gatggagtac cctgaggcta taactcgcct
     6361 agtgacaggg gcccagaggc cccccgaccc agctcctgct ccactggggg ccccggggct
     6421 ccccaatggc ctcctttcag gagatgaaga cttctcctcc attgcggaca tggacttctc
     6481 agccctgctg agtcagatca gctcctaaga tcaagcttat cgataatcaa cctctggatt
     6541 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     6601 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     6661 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     6721 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     6781 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     6841 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     6901 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct
     6961 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     7021 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     7081 cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgacct cgaggttcgc
     7141 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     7201 gggactggaa gggctaattc actcccaacg aagacaagat gggatcaatt caccatggga
     7261 ataacttcgt atagcataca ttatacgaag ttatgctgct ttttgcttgt actgggtctc
     7321 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     7381 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     7441 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagcatct
     7501 agttgaatcg ccagtgtgtc gagctagcag atctttttcc ctctgccaaa aattatgggg
     7561 acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt attttcattg
     7621 caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc
     7681 atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc atatgctggc
     7741 tgccatgaac aaaggtggct ataaagaggt catcagtata tgaaacagcc ccctgctgtc
     7801 cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat attttgtttt
     7861 gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact agccagattt
     7921 ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg aagatccctc
     7981 gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     8041 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     8101 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     8161 aaacctgtcg tgccagcgga tccgcatctc aattagtcag caaccatagt cccgccccta
     8221 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     8281 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     8341 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctaacttg tttattgcag
     8401 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     8461 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcc
     8521 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     8581 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     8641 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     8701 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     8761 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     8821 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     8881 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     8941 ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     9001 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     9061 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     9121 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     9181 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     9241 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     9301 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     9361 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     9421 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     9481 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     9541 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     9601 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     9661 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     9721 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     9781 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     9841 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     9901 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     9961 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
    10021 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
    10081 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
    10141 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
    10201 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
    10261 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
    10321 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
    10381 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
    10441 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
    10501 gccacctg
//
