LOCUS       sequence-215780-       11216 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TET-inducible expression of Erythropoietin receptor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11216)
  TITLE     Salmon Lab plasmids submitted
REFERENCE   2  (bases 1 to 11216)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCWXPGR-pTF-EpoR
FEATURES             Location/Qualifiers
     source          1..11216
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             385..642
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    689..814
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1306..1423
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    1783..2016
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2201..2245
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     protein_bind    2522..2542
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    2569..2587
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    2610..2628
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    2774..2792
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    2854..2878
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4408..4432)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4450..4960
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4879..4897
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      4976..4985
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4982..4984,4985..4987,4988..5698)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4982..4984,4985..4987,4988..5698)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5027..5048)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5288..5307)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5635..5656
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5714..5773
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             6842..7213
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     misc_feature    7233..7821
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7286..7306)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7704..7715)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7824..7840)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7825..7841)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    complement(7969..8002)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             8022..8202
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8240..8259)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    8305..8360
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8359..8378)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(8719..8735)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8719..8735)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8732..8754)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8743..8759
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8767..8773,8774..8791,8792..8797))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8812..8833
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8892..9087
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8938..9073
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    9093..9227
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9130..9149)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9184..9203
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9295..9312)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9466..10054)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9546..9565)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10225..11016,11017..11085))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10848..10867
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11086..11190)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggaggcg tggcctgggc gggactgggg agtggcgagc
      421 cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc
      481 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
      541 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
      601 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
      661 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
      721 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
      781 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
      841 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
      901 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
      961 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     1021 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     1081 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaaaaa
     1141 agcacagcaa gcagcaggat cttcagacct ggaaattccc tacaatcccc aaagtcaagg
     1201 agtagtagaa tctatgaata aagaattaaa gaaaattata ggacaggtaa gagatcaggc
     1261 tgaacatctt aagacagcag tacaaatggc agtattcatc cacaatttta aaagaaaagg
     1321 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     1381 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     1441 cagcagaaat ccactttgga aaggaccagc aaagctcctc tggaaaggtg aaggggcagt
     1501 agtaatacaa gataatagtg acataaaagt agtgccaaga agaaaagcaa agatcattag
     1561 ggattatgga aaacagatgg caggtgatga ttgtgtggca agtagacagg atgaggatta
     1621 gaacatggaa aagtttagta aaacaccata aggaggagat atgagggaca attggagaag
     1681 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1741 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1801 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1861 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1921 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1981 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     2041 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     2101 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     2161 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     2221 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     2281 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     2341 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     2401 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     2461 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgctagt gattatcgga
     2521 tcaactttgt atagaaaagt tgggtacaac tcccactaga catatcctac tctatcattg
     2581 atagagtgac ccttccatag acatatccta ctctatcatt gatagagtga cccttccact
     2641 aggttcactt ttctctatca ttgataggga gtggtaaaca attgatggac aaaatctatc
     2701 aatgatagag agtgcatgtt actagtattg ttcttctcta tcattgatag tgactttgcc
     2761 taatcactta gcaactctat cattgataga gttctagtat tagcaaggcc tttcctctat
     2821 ataaggaagt tcatttcatt tggagaagat cccccaagtt tgtacaaaaa agcaggcttc
     2881 accatggacc aactcagggt ggcccgctgg cctcgggtta gccccctgtg tctcctactt
     2941 gctggggcag cctgggcatc ctcacccagc ctcccggacc ccaagtttga gagcaaagcc
     3001 gccctgctgg catcccgggg ctccgaagaa cttctatgct tcacccagcg cttggaagac
     3061 ttggtgtgtt tctgggagga agcggcgaac tccgggatgg gcttcaacta cagcttctct
     3121 taccagctcg aaggtgaatc aagaaagtca tgtcgcctgc accaggcgcc caccgtccgc
     3181 ggctccatgc gtttctggtg ttcactgccg accgcggaca cgtcgagttt tgtgccactg
     3241 gagctgcagg tgaccgaggc ctccggatct cctcgctacc accgcatcat ccatatcaat
     3301 gaagtagtgc tcctggacgc ccccgcgggg ctgctggcgc gccgagcaga agagggcagc
     3361 cacgtggtgc tgcggtggct gccacctccc ggggctccta tgaccaccca catccgctac
     3421 gaggtggacg tgtcagcagg caaccgggct ggggggacac aaagggtgga ggtcctggaa
     3481 ggccgcactg agtgtgtcct gagcaacctg cggggcggga cgcgctacac cttcgctgtt
     3541 cgagcacgca tggccgagcc gagcttcagc ggattctgga gcgcctggtc tgagcccgcg
     3601 tcgctactga ctgctagcga cttggaccct ctcatcttga cgctgtctct cattctcgtc
     3661 ctcatctcac tgttgctgac tgtgctggcc ctgctgtccc accgccgggc tctgcggcag
     3721 aagatctggc ctggcatccc aagcccagag aatgagtttg agggtctctt caccacccac
     3781 aagggtaact tccagctatg gctgttgcaa cgcgatggct gtctgtggtg gagcccaagt
     3841 agccccttcc ctgaggatcc acctgcccac ctagaggtcc tctcagagcg acactgggga
     3901 gtgactcagg ctggggatgc aggggcagag gacaaggggc ccttactgga gccagtgggc
     3961 agtgagcggg cccaggacac ctacctggta ttggatgaat ggttgctacc ccggtgccca
     4021 tgcagtgaga acctctctgg gcctggggac agtgtagacc ctgcgactat ggatgaaggt
     4081 tcagaaacat cttcctgccc ctctgacttg gcttcaaagc ccaggccaga gggcacctcg
     4141 ccttccagct ttgagtacac catcctggac cccagctcta agctcctgtg ccctcgggca
     4201 ctgcctcctg agctaccccc cactccacct cacctgaagt acctgtacct tgtggtgtcc
     4261 gattccggca tctcaacaga ttacagctca gggggctccc agggagtcca cggggactca
     4321 tctgacggcc cctactccca cccctatgag aatagccttg ttccagacac agagcctctg
     4381 cggcccagct acgtggcctg ctcctagacc cagctttctt gtacaaagtg gtgataatcg
     4441 aattcccacg gggttggggt tgcgcctttt ccaaggcagc cctgggtttg cgcagggacg
     4501 cggctgctct gggcgtggtt ccgggaaacg cagcggcgcc gaccctgggt ctcgcacatt
     4561 cttcacgtcc gttcgcagcg tcacccggat cttcgccgct acccttgtgg gccccccggc
     4621 gacgcttcct gctccgcccc taagtcggga aggttccttg cggttcgcgg cgtgccggac
     4681 gtgacaaacg gaagccgcac gtctcactag taccctcgca gacggacagc gccagggagc
     4741 aatggcagcg cgccgaccgc gatgggctgt ggccaatagc ggctgctcag cggggcgcgc
     4801 cgagagcagc ggccgggaag gggcggtgcg ggaggcgggg tgtggggcgg tagtgtgggc
     4861 cctgttcctg cccgcgcggt gttccgcatt ctgcaagcct ccggagcgca cgtcggcagt
     4921 cggctccctc gttgaccgaa tcaccgacct ctctccccag ggggatccac cggtcgccac
     4981 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     5041 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     5101 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     5161 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
     5221 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     5281 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     5341 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     5401 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
     5461 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     5521 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     5581 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
     5641 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaaggc
     5701 tagcggatca ggtcaatgta ctaactacgc tttgttgaag cttgccggcg atgttgaaag
     5761 taaccccggt cctggatcca tgtccagact ggacaagagc aaagtcataa acggcgctct
     5821 ggaattactc aatggagtcg gtatcgaagg cctgacgaca aggaaactcg ctcaaaagct
     5881 gggagttgag cagcctaccc tgtactggca cgtgaagaac aagcgggccc tgctcgatgc
     5941 cctgccaatc gagatgctgg acaggcatca tacccacttc tgccccctgg aaggcgagtc
     6001 atggcaagac tttctgcgga acaacgccaa gtcattccgc tgtgctctcc tctcacatcg
     6061 cgacggggct aaagtgcatc tcggcacccg cccaacagag aaacagtacg aaaccctgga
     6121 aaatcagctc gcgttcctgt gtcagcaagg cttctccctg gagaacgcac tgtacgctct
     6181 gtccgccgtg ggccacttta cactgggctg cgtattggag gaacaggagc atcaagtagc
     6241 aaaagaggaa agagagacac ctaccaccga ttctatgccc ccacttctga gacaagcaat
     6301 tgagctgttc gaccggcagg gagccgaacc tgccttcctt ttcggcctgg aactaatcat
     6361 atgtggcctg gagaaacagc taaagtgcga aagcggcggg ccggcctcga gcgaattcca
     6421 gtacctgcca gatacagacg atcgtcaccg gattgaggag aaacgtaaaa ggacatatga
     6481 gaccttcaag agcatcatga agaagagtcc tttcagcgga cccaccgacc cccggcctcc
     6541 acctcgacgc attgctgtgc cttcccgcag ctcagcttct gtccccaagc cagcacccca
     6601 gccctatccc tttacgtcat ccctgagcac catcaactat gatgagtttc ccaccatggt
     6661 gtttccttct gggcagatca gccaggcctc ggccttggcc ccggcccctc cccaagtcct
     6721 gccccaggct ccagcccctg cccctgctcc agccatggta tcagctctgg cccaggcccc
     6781 agcccctgtc ccagtcctag ccccaggccc tcctcaggct gtggccccac ctgcccccaa
     6841 gcccacccag gctggggaag gaacgctgtc agaggccctg ctgcagctgc agtttgatga
     6901 tgaagacctg ggggccttgc ttggcaacag cacagaccca gctgtgttca cagacctggc
     6961 atccgtcgac aactccgagt ttcagcagct gctgaaccag ggcatacctg tggcccccca
     7021 cacaactgag cccatgctga tggagtaccc tgaggctata actcgcctag tgacaggggc
     7081 ccagaggccc cccgacccag ctcctgctcc actgggggcc ccggggctcc ccaatggcct
     7141 cctttcagga gatgaagact tctcctccat tgcggacatg gacttctcag ccctgctgag
     7201 tcagatcagc tcctaagatc aagcttatcg ataatcaacc tctggattac aaaatttgtg
     7261 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     7321 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     7381 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     7441 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     7501 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     7561 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     7621 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     7681 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     7741 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     7801 ccctttgggc cgcctccccg catcgatacc gtcgacctcg aggttcgctt taagaccaat
     7861 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     7921 gctaattcac tcccaacgaa gacaagatgg gatcaattca ccatgggaat aacttcgtat
     7981 agcatacatt atacgaagtt atgctgcttt ttgcttgtac tgggtctctc tggttagacc
     8041 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     8101 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     8161 gatccctcag acccttttag tcagtgtgga aaatctctag cagcatctag ttgaatcgcc
     8221 agtgtgtcga gctagcagat ctttttccct ctgccaaaaa ttatggggac atcatgaagc
     8281 cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca atagtgtgtt
     8341 ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat ttaaaacatc
     8401 agaatgagta tttggtttag agtttggcaa catatgccat atgctggctg ccatgaacaa
     8461 aggtggctat aaagaggtca tcagtatatg aaacagcccc ctgctgtcca ttccttattc
     8521 catagaaaag ccttgacttg aggttagatt ttttttatat tttgttttgt gttatttttt
     8581 tctttaacat ccctaaaatt ttccttacat gttttactag ccagattttt cctcctctcc
     8641 tgactactcc cagtcatagc tgtccctctt ctcttatgaa gatccctcga cctgcagccc
     8701 aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     8761 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     8821 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     8881 ccagcggatc cgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     8941 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     9001 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     9061 ttttttggag gcctaggctt ttgcaaaaag ctaacttgtt tattgcagct tataatggtt
     9121 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     9181 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatccgc tgcattaatg
     9241 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     9301 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     9361 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     9421 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     9481 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     9541 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     9601 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     9661 atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     9721 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     9781 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     9841 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     9901 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     9961 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
    10021 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
    10081 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
    10141 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
    10201 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
    10261 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
    10321 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
    10381 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
    10441 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
    10501 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
    10561 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
    10621 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
    10681 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
    10741 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
    10801 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
    10861 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
    10921 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
    10981 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
    11041 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
    11101 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
    11161 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctg
//
