LOCUS       sequence-215782-       12047 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TET-inducible expression of ERK:MEK fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12047)
  TITLE     Salmon Lab plasmids submitted
REFERENCE   2  (bases 1 to 12047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCWXPGR-pTF-ERK:MEK
FEATURES             Location/Qualifiers
     source          1..12047
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             385..642
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    689..814
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1306..1423
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    1783..2016
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2201..2245
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     protein_bind    2522..2542
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    2569..2587
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    2610..2628
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    2774..2792
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    2854..2878
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    2887..2911
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2915..2924
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3952..3987
                     /codon_start=1
                     /product="epitope tag derived from MAP kinase 2"
                     /label=Tag-100
                     /translation="EETARFQPGYRS"
     protein_bind    complement(5239..5263)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5281..5791
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     5710..5728
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      5807..5816
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5813..5815,5816..5818,5819..6529)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5813..5815,5816..5818,5819..6529)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5858..5879)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6119..6138)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6466..6487
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             6545..6604
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             7673..8044
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     misc_feature    8064..8652
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8117..8137)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8535..8546)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8655..8671)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8656..8672)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    complement(8800..8833)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             8853..9033
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9071..9090)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    9136..9191
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(9190..9209)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(9550..9566)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9550..9566)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9563..9585)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9574..9590
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9598..9604,9605..9622,9623..9628))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9643..9664
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        9723..9918
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9769..9904
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    9924..10058
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9961..9980)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10015..10034
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10126..10143)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10297..10885)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10377..10396)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11056..11847,11848..11916))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11679..11698
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11917..12021)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggaggcg tggcctgggc gggactgggg agtggcgagc
      421 cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc
      481 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
      541 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
      601 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
      661 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
      721 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
      781 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
      841 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
      901 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
      961 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     1021 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     1081 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaaaaa
     1141 agcacagcaa gcagcaggat cttcagacct ggaaattccc tacaatcccc aaagtcaagg
     1201 agtagtagaa tctatgaata aagaattaaa gaaaattata ggacaggtaa gagatcaggc
     1261 tgaacatctt aagacagcag tacaaatggc agtattcatc cacaatttta aaagaaaagg
     1321 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     1381 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     1441 cagcagaaat ccactttgga aaggaccagc aaagctcctc tggaaaggtg aaggggcagt
     1501 agtaatacaa gataatagtg acataaaagt agtgccaaga agaaaagcaa agatcattag
     1561 ggattatgga aaacagatgg caggtgatga ttgtgtggca agtagacagg atgaggatta
     1621 gaacatggaa aagtttagta aaacaccata aggaggagat atgagggaca attggagaag
     1681 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1741 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1801 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1861 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1921 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1981 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     2041 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     2101 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     2161 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     2221 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     2281 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     2341 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     2401 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     2461 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgctagt gattatcgga
     2521 tcaactttgt atagaaaagt tgggtacaac tcccactaga catatcctac tctatcattg
     2581 atagagtgac ccttccatag acatatccta ctctatcatt gatagagtga cccttccact
     2641 aggttcactt ttctctatca ttgataggga gtggtaaaca attgatggac aaaatctatc
     2701 aatgatagag agtgcatgtt actagtattg ttcttctcta tcattgatag tgactttgcc
     2761 taatcactta gcaactctat cattgataga gttctagtat tagcaaggcc tttcctctat
     2821 ataaggaagt tcatttcatt tggagaagat cccccaagtt tgtacaaaaa agcaggcttc
     2881 accatgccaa ctttgtacaa aaaagcaggc ttcaccatgg cggcggcggc ggcgggcccg
     2941 gagatggtcc gcgggcaggt gttcgacgtg gggccgcgct acactaatct ctcgtacatc
     3001 ggagaaggcg cctacggcat ggtttgttct gcttatgata atctcaacaa agttcgagtt
     3061 gctatcaaga aaatcagtcc ttttgagcac cagacctact gtcagagaac cctgagagag
     3121 ataaaaatcc tactgcgctt cagacatgag aacatcatcg gcatcaatga catcatccgg
     3181 gcaccaacca ttgagcagat gaaagatgta tatatagtac aggacctcat ggagacagat
     3241 ctttacaagc tcttgaagac acagcacctc agcaatgatc atatctgcta ttttctttat
     3301 cagatcctga gaggattaaa gtatatacat tcagctaatg ttctgcaccg tgacctcaag
     3361 ccttccaacc tcctgctgaa caccacttgt gatctcaaga tctgtgactt tggccttgcc
     3421 cgtgttgcag atccagacca tgatcataca gggttcttga cagagtatgt agccacgcgt
     3481 tggtacagag ctccagaaat tatgttgaat tccaagggtt ataccaagtc cattgatatt
     3541 tggtctgtgg gctgcatcct ggcagagatg ctatccaaca ggcctatctt tccaggaaag
     3601 cattaccttg accagctgaa tcacatcctg ggtattcttg gatctccatc acaggaagat
     3661 ctgaattgta taataaattt aaaagctaga aactatttgc tttctctccc gcacaaaaat
     3721 aaggtgccgt ggaacagatt gttcccaaac gctgactcca aagctctgga tttactggat
     3781 aaaatgttga catttaaccc tcacaagagg attgaagttg aacaggctct ggcccacccg
     3841 tacctggagc agtattatga cccaagtgat gagcccattg ctgaagcacc attcaagttt
     3901 gacatggagc tggacgactt acctaaggag aagctcaaag aactcatttt tgaagagact
     3961 gctcgattcc agccaggata cagatctgaa ggcgagggtg aaggcgaggg tgaaagcgga
     4021 tccggcggag ttggaagcgc gttacccggg tccaaaatgc ccaagaagaa gccgacgccc
     4081 atccagctga acccggcccc cgacggctct gcagttaacg ggaccagctc tgcggagacc
     4141 aacttggagg ccgcgcagaa gaaggcggag gaggcagagg ctgatgagca gcagcgaaag
     4201 cgccttgagg cctttcttac ccagaagcag aaggtgggag aactgaagga tgacgacttt
     4261 gagaagatca gtgagctggg ggctggcaat ggcggtgtgg tgttcaaggt ctcccacaag
     4321 ccttctggcc tggtcatggc cagaaagcta attcatctgg agatcaaacc cgcaatccgg
     4381 aaccagatca taagggagct gcaggttctg catgagtgca actctccgta catcgtgggc
     4441 ttctatggtg cgttctacag cgatggcgag atcagtatct gcatggagca catggatgga
     4501 ggttctctgg atcaagtcct gaagaaagct ggaagaattc ctgaacaaat tttaggaaaa
     4561 gttagcattg ctgtaataaa aggcctgaca tatctgaggg agaagcacaa gatcatgcac
     4621 agagatgtca agccctccaa catcctagtc aactcccgtg gggagatcaa gctctgtgac
     4681 tttggggtca gcgggcagct catcgactcc atggccaact ccttcgtggg cacaaggtcc
     4741 tacatgtcgc cagaaagact ccaggggact cattactctg tgcagtcaga catctggagc
     4801 atgggactgt ctctggtaga gatggcggtt gggaggtatc ccatccctcc tccagatgcc
     4861 aaggagctgg agctgatgtt tgggtgccag gtggaaggag atgcggctga gaccccaccc
     4921 aggccaagga cccccgggag gccccttagc tcatacggaa tggacagccg acctcccatg
     4981 gcaatttttg agttgttgga ttacatagtc aacgagcctc ctccaaaact gcccagtgga
     5041 gtgttcagtc tggaatttca agattttgtg aataaatgct taataaaaaa ccccgcagag
     5101 agagcagatt tgaagcaact catggttcat gcttttatca agagatctga tgctgaggaa
     5161 gtggattttg caggttggct ctgctccacc atcggcctta accagcccag cacaccaacc
     5221 catgctgctg gcgtctaaac ccagctttct tgtacaaagt ggtgataatc gaattcccac
     5281 ggggttgggg ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc
     5341 tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc
     5401 cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc
     5461 tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac
     5521 ggaagccgca cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc
     5581 gcgccgaccg cgatgggctg tggccaatag cggctgctca gcggggcgcg ccgagagcag
     5641 cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct
     5701 gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct
     5761 cgttgaccga atcaccgacc tctctcccca gggggatcca ccggtcgcca ccatggtgag
     5821 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     5881 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     5941 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     6001 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
     6061 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     6121 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     6181 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     6241 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
     6301 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     6361 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     6421 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     6481 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagg ctagcggatc
     6541 aggtcaatgt actaactacg ctttgttgaa gcttgccggc gatgttgaaa gtaaccccgg
     6601 tcctggatcc atgtccagac tggacaagag caaagtcata aacggcgctc tggaattact
     6661 caatggagtc ggtatcgaag gcctgacgac aaggaaactc gctcaaaagc tgggagttga
     6721 gcagcctacc ctgtactggc acgtgaagaa caagcgggcc ctgctcgatg ccctgccaat
     6781 cgagatgctg gacaggcatc atacccactt ctgccccctg gaaggcgagt catggcaaga
     6841 ctttctgcgg aacaacgcca agtcattccg ctgtgctctc ctctcacatc gcgacggggc
     6901 taaagtgcat ctcggcaccc gcccaacaga gaaacagtac gaaaccctgg aaaatcagct
     6961 cgcgttcctg tgtcagcaag gcttctccct ggagaacgca ctgtacgctc tgtccgccgt
     7021 gggccacttt acactgggct gcgtattgga ggaacaggag catcaagtag caaaagagga
     7081 aagagagaca cctaccaccg attctatgcc cccacttctg agacaagcaa ttgagctgtt
     7141 cgaccggcag ggagccgaac ctgccttcct tttcggcctg gaactaatca tatgtggcct
     7201 ggagaaacag ctaaagtgcg aaagcggcgg gccggcctcg agcgaattcc agtacctgcc
     7261 agatacagac gatcgtcacc ggattgagga gaaacgtaaa aggacatatg agaccttcaa
     7321 gagcatcatg aagaagagtc ctttcagcgg acccaccgac ccccggcctc cacctcgacg
     7381 cattgctgtg ccttcccgca gctcagcttc tgtccccaag ccagcacccc agccctatcc
     7441 ctttacgtca tccctgagca ccatcaacta tgatgagttt cccaccatgg tgtttccttc
     7501 tgggcagatc agccaggcct cggccttggc cccggcccct ccccaagtcc tgccccaggc
     7561 tccagcccct gcccctgctc cagccatggt atcagctctg gcccaggccc cagcccctgt
     7621 cccagtccta gccccaggcc ctcctcaggc tgtggcccca cctgccccca agcccaccca
     7681 ggctggggaa ggaacgctgt cagaggccct gctgcagctg cagtttgatg atgaagacct
     7741 gggggccttg cttggcaaca gcacagaccc agctgtgttc acagacctgg catccgtcga
     7801 caactccgag tttcagcagc tgctgaacca gggcatacct gtggcccccc acacaactga
     7861 gcccatgctg atggagtacc ctgaggctat aactcgccta gtgacagggg cccagaggcc
     7921 ccccgaccca gctcctgctc cactgggggc cccggggctc cccaatggcc tcctttcagg
     7981 agatgaagac ttctcctcca ttgcggacat ggacttctca gccctgctga gtcagatcag
     8041 ctcctaagat caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga
     8101 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     8161 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     8221 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     8281 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     8341 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     8401 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     8461 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     8521 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     8581 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     8641 ccgcctcccc gcatcgatac cgtcgacctc gaggttcgct ttaagaccaa tgacttacaa
     8701 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     8761 ctcccaacga agacaagatg ggatcaattc accatgggaa taacttcgta tagcatacat
     8821 tatacgaagt tatgctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     8881 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     8941 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     9001 gaccctttta gtcagtgtgg aaaatctcta gcagcatcta gttgaatcgc cagtgtgtcg
     9061 agctagcaga tctttttccc tctgccaaaa attatgggga catcatgaag ccccttgagc
     9121 atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt
     9181 tgtgtctctc actcggaagg acatatggga gggcaaatca tttaaaacat cagaatgagt
     9241 atttggttta gagtttggca acatatgcca tatgctggct gccatgaaca aaggtggcta
     9301 taaagaggtc atcagtatat gaaacagccc cctgctgtcc attccttatt ccatagaaaa
     9361 gccttgactt gaggttagat tttttttata ttttgttttg tgttattttt ttctttaaca
     9421 tccctaaaat tttccttaca tgttttacta gccagatttt tcctcctctc ctgactactc
     9481 ccagtcatag ctgtccctct tctcttatga agatccctcg acctgcagcc caagcttggc
     9541 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     9601 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     9661 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagcggat
     9721 ccgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     9781 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     9841 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     9901 ggcctaggct tttgcaaaaa gctaacttgt ttattgcagc ttataatggt tacaaataaa
     9961 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
    10021 tgtccaaact catcaatgta tcttatcatg tctggatccg ctgcattaat gaatcggcca
    10081 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
    10141 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
    10201 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
    10261 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
    10321 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
    10381 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
    10441 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg
    10501 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
    10561 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
    10621 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
    10681 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
    10741 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
    10801 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
    10861 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
    10921 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
    10981 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
    11041 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
    11101 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
    11161 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
    11221 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
    11281 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
    11341 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
    11401 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    11461 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    11521 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    11581 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    11641 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    11701 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    11761 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    11821 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    11881 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    11941 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    12001 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctg
//
