LOCUS       sequence-215783-       10442 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TET-inducible expression of BDNF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10442)
  TITLE     Salmon Lab plasmids submitted
REFERENCE   2  (bases 1 to 10442)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCWXPGR-pTF-BDNF
FEATURES             Location/Qualifiers
     source          1..10442
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             385..642
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    689..814
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1306..1423
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    1783..2016
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2201..2245
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     protein_bind    2522..2542
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    2569..2587
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    2610..2628
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    2774..2792
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    2854..2878
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3634..3658)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        3676..4186
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4105..4123
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      4202..4211
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4208..4210,4211..4213,4214..4924)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4208..4210,4211..4213,4214..4924)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4253..4274)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4514..4533)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4861..4882
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4940..4999
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             6068..6439
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     misc_feature    6459..7047
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6512..6532)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6930..6941)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7050..7066)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7051..7067)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    complement(7195..7228)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             7248..7428
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7466..7485)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7531..7586
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7585..7604)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7945..7961)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7945..7961)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7958..7980)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7969..7985
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7993..7999,8000..8017,8018..8023))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8038..8059
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8118..8313
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8164..8299
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    8319..8453
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8356..8375)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8410..8429
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8521..8538)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8692..9280)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8772..8791)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9451..10242,10243..10311))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10074..10093
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10312..10416)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggaggcg tggcctgggc gggactgggg agtggcgagc
      421 cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc
      481 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
      541 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
      601 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
      661 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
      721 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
      781 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
      841 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
      901 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
      961 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     1021 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     1081 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaaaaa
     1141 agcacagcaa gcagcaggat cttcagacct ggaaattccc tacaatcccc aaagtcaagg
     1201 agtagtagaa tctatgaata aagaattaaa gaaaattata ggacaggtaa gagatcaggc
     1261 tgaacatctt aagacagcag tacaaatggc agtattcatc cacaatttta aaagaaaagg
     1321 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     1381 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     1441 cagcagaaat ccactttgga aaggaccagc aaagctcctc tggaaaggtg aaggggcagt
     1501 agtaatacaa gataatagtg acataaaagt agtgccaaga agaaaagcaa agatcattag
     1561 ggattatgga aaacagatgg caggtgatga ttgtgtggca agtagacagg atgaggatta
     1621 gaacatggaa aagtttagta aaacaccata aggaggagat atgagggaca attggagaag
     1681 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1741 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1801 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1861 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1921 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1981 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     2041 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     2101 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     2161 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     2221 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     2281 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     2341 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     2401 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     2461 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgctagt gattatcgga
     2521 tcaactttgt atagaaaagt tgggtacaac tcccactaga catatcctac tctatcattg
     2581 atagagtgac ccttccatag acatatccta ctctatcatt gatagagtga cccttccact
     2641 aggttcactt ttctctatca ttgataggga gtggtaaaca attgatggac aaaatctatc
     2701 aatgatagag agtgcatgtt actagtattg ttcttctcta tcattgatag tgactttgcc
     2761 taatcactta gcaactctat cattgataga gttctagtat tagcaaggcc tttcctctat
     2821 ataaggaagt tcatttcatt tggagaagat cccccaagtt tgtacaaaaa agcaggcttc
     2881 accatgacca tccttttcct tactatggtt atttcatact tcggttgcat gaaggcggcg
     2941 cccatgaaag aagtaaacgt ccacggacaa ggcaacttgg cctacccagg tgtgcggacc
     3001 catgggactc tggagagcgt gaatgggccc agggcaggtt cgagaggtct gacgacgaca
     3061 tcactggctg acacttttga gcacgtcatc gaagagctgc tggatgagga ccagaaggtt
     3121 cggcccaacg aagaaaacca taaggacgcg gacttgtaca cttcccgggt gatgctcagc
     3181 agtcaagtgc ctttggagcc tcctctactc tttctgctgg aggaatacaa aaattacctg
     3241 gatgccgcaa acatgtctat gagggttcgg cgccactccg accctgcccg ccgtggggag
     3301 ctgagcgtgt gtgacagtat tagcgagtgg gtcacagcgg cagataaaaa gactgcagtg
     3361 gacatgtctg gcgggacggt cacagtccta gagaaagtcc cggtatccaa aggccaactg
     3421 aagcagtatt tctacgagac caagtgtaat cccatgggtt acaccaagga aggctgcagg
     3481 ggcatagaca aaaggcactg gaactcgcaa tgccgaacta cccaatcgta tgttcgggcc
     3541 cttactatgg atagcaaaaa gagaattggc tggcgattca taaggataga cacttcctgt
     3601 gtatgtacac tgaccattaa gaggggaaga tagacccagc tttcttgtac aaagtggtga
     3661 taatcgaatt cccacggggt tggggttgcg ccttttccaa ggcagccctg ggtttgcgca
     3721 gggacgcggc tgctctgggc gtggttccgg gaaacgcagc ggcgccgacc ctgggtctcg
     3781 cacattcttc acgtccgttc gcagcgtcac ccggatcttc gccgctaccc ttgtgggccc
     3841 cccggcgacg cttcctgctc cgcccctaag tcgggaaggt tccttgcggt tcgcggcgtg
     3901 ccggacgtga caaacggaag ccgcacgtct cactagtacc ctcgcagacg gacagcgcca
     3961 gggagcaatg gcagcgcgcc gaccgcgatg ggctgtggcc aatagcggct gctcagcggg
     4021 gcgcgccgag agcagcggcc gggaaggggc ggtgcgggag gcggggtgtg gggcggtagt
     4081 gtgggccctg ttcctgcccg cgcggtgttc cgcattctgc aagcctccgg agcgcacgtc
     4141 ggcagtcggc tccctcgttg accgaatcac cgacctctct ccccaggggg atccaccggt
     4201 cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     4261 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     4321 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     4381 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     4441 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     4501 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     4561 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     4621 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     4681 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     4741 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     4801 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     4861 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     4921 caaggctagc ggatcaggtc aatgtactaa ctacgctttg ttgaagcttg ccggcgatgt
     4981 tgaaagtaac cccggtcctg gatccatgtc cagactggac aagagcaaag tcataaacgg
     5041 cgctctggaa ttactcaatg gagtcggtat cgaaggcctg acgacaagga aactcgctca
     5101 aaagctggga gttgagcagc ctaccctgta ctggcacgtg aagaacaagc gggccctgct
     5161 cgatgccctg ccaatcgaga tgctggacag gcatcatacc cacttctgcc ccctggaagg
     5221 cgagtcatgg caagactttc tgcggaacaa cgccaagtca ttccgctgtg ctctcctctc
     5281 acatcgcgac ggggctaaag tgcatctcgg cacccgccca acagagaaac agtacgaaac
     5341 cctggaaaat cagctcgcgt tcctgtgtca gcaaggcttc tccctggaga acgcactgta
     5401 cgctctgtcc gccgtgggcc actttacact gggctgcgta ttggaggaac aggagcatca
     5461 agtagcaaaa gaggaaagag agacacctac caccgattct atgcccccac ttctgagaca
     5521 agcaattgag ctgttcgacc ggcagggagc cgaacctgcc ttccttttcg gcctggaact
     5581 aatcatatgt ggcctggaga aacagctaaa gtgcgaaagc ggcgggccgg cctcgagcga
     5641 attccagtac ctgccagata cagacgatcg tcaccggatt gaggagaaac gtaaaaggac
     5701 atatgagacc ttcaagagca tcatgaagaa gagtcctttc agcggaccca ccgacccccg
     5761 gcctccacct cgacgcattg ctgtgccttc ccgcagctca gcttctgtcc ccaagccagc
     5821 accccagccc tatcccttta cgtcatccct gagcaccatc aactatgatg agtttcccac
     5881 catggtgttt ccttctgggc agatcagcca ggcctcggcc ttggccccgg cccctcccca
     5941 agtcctgccc caggctccag cccctgcccc tgctccagcc atggtatcag ctctggccca
     6001 ggccccagcc cctgtcccag tcctagcccc aggccctcct caggctgtgg ccccacctgc
     6061 ccccaagccc acccaggctg gggaaggaac gctgtcagag gccctgctgc agctgcagtt
     6121 tgatgatgaa gacctggggg ccttgcttgg caacagcaca gacccagctg tgttcacaga
     6181 cctggcatcc gtcgacaact ccgagtttca gcagctgctg aaccagggca tacctgtggc
     6241 cccccacaca actgagccca tgctgatgga gtaccctgag gctataactc gcctagtgac
     6301 aggggcccag aggccccccg acccagctcc tgctccactg ggggccccgg ggctccccaa
     6361 tggcctcctt tcaggagatg aagacttctc ctccattgcg gacatggact tctcagccct
     6421 gctgagtcag atcagctcct aagatcaagc ttatcgataa tcaacctctg gattacaaaa
     6481 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     6541 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     6601 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     6661 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     6721 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     6781 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     6841 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     6901 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     6961 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     7021 ggatctccct ttgggccgcc tccccgcatc gataccgtcg acctcgaggt tcgctttaag
     7081 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     7141 ggaagggcta attcactccc aacgaagaca agatgggatc aattcaccat gggaataact
     7201 tcgtatagca tacattatac gaagttatgc tgctttttgc ttgtactggg tctctctggt
     7261 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     7321 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     7381 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagc atctagttga
     7441 atcgccagtg tgtcgagcta gcagatcttt ttccctctgc caaaaattat ggggacatca
     7501 tgaagcccct tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag
     7561 tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa
     7621 aacatcagaa tgagtatttg gtttagagtt tggcaacata tgccatatgc tggctgccat
     7681 gaacaaaggt ggctataaag aggtcatcag tatatgaaac agccccctgc tgtccattcc
     7741 ttattccata gaaaagcctt gacttgaggt tagatttttt ttatattttg ttttgtgtta
     7801 tttttttctt taacatccct aaaattttcc ttacatgttt tactagccag atttttcctc
     7861 ctctcctgac tactcccagt catagctgtc cctcttctct tatgaagatc cctcgacctg
     7921 cagcccaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     7981 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     8041 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     8101 gtcgtgccag cggatccgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     8161 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     8221 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     8281 ggaggctttt ttggaggcct aggcttttgc aaaaagctaa cttgtttatt gcagcttata
     8341 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     8401 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atccgctgca
     8461 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     8521 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     8581 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     8641 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     8701 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     8761 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     8821 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     8881 ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     8941 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     9001 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     9061 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     9121 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     9181 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     9241 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     9301 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     9361 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     9421 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     9481 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     9541 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     9601 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     9661 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     9721 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     9781 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     9841 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     9901 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     9961 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
    10021 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
    10081 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
    10141 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
    10201 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
    10261 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
    10321 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
    10381 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
    10441 tg
//
