LOCUS       sequence-215787-       12476 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive expression of the E2F1 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12476)
  AUTHORS   Rady B, Chen Y, Vaca P, Wang Q, Wang Y, Salmon P, Oberholzer J
  TITLE     Overexpression of E2F3 promotes proliferation of functional human 
            beta cells without induction of apoptosis.
  JOURNAL   Cell Cycle. 2013 Aug 15;12(16):2691-702. doi: 10.4161/cc.25834. Epub
            2013 Jul 29.
  PUBMED    23907129
REFERENCE   2  (bases 1 to 12476)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.4161/cc.25834"; 
            journalName: "Cell Cycle"; date: "2013-08-15- 15"; volume: "12"; 
            issue: "16"; pages: "2691-702"
COMMENT     Sequence Label: pWPI-E2F1
FEATURES             Location/Qualifiers
     source          1..12476
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1299..1532
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1717..1761
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2119..3296
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2350..3287
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3244..3264
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3344..3461
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    3492..3516
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4836..4860)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4884..5451
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4902..5453
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5046..5063)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5273..5292
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(5456..5458,5459..5461,5462..6172)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5456..5458,5459..5461,5462..6172)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5501..5522)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5762..5781)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6109..6130
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(6250..6266)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6251..6267)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    6280..6868
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6333..6353)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6751..6762)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(7037..7070)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             7090..7270
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(7295..7313)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7296..7313)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7603..7622)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7836..7855)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7955..7977
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8015..8033)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8101..8205
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8206..8274,8275..9066)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8424..8443)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9237..9825
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9726..9745
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9979..9996
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10066..10086)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10071..10400
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10251..10386
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          11534..11599
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11729..11749
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    12174..12308
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12211..12230)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12265..12284
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     1201 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     1261 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     1321 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     1381 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     1441 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     1501 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     1561 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     1621 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     1681 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     1741 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     1801 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     1861 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     1921 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     1981 gacagatcca ttcgattagt gaacggatct cgacggtatc gatgtcgacg ataagctttg
     2041 caaagatgga taaagtttta aacagagagg aatctttgca gctaatggac cttctaggtc
     2101 ttgaaaggag tgggaattgg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca
     2161 gtccccgaga agttgggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc
     2221 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga
     2281 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc
     2341 agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc
     2401 ccttgcgtgc cttgaattac ttccactggc tgcagtacgt gattcttgat cccgagcttc
     2461 gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg
     2521 cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc
     2581 gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg
     2641 cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta
     2701 tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg
     2761 cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc
     2821 ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc
     2881 tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag
     2941 ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa
     3001 ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc
     3061 cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta ggttgggggg
     3121 aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag
     3181 cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca
     3241 ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgaggaa
     3301 tttcgacatt taaatttaat taatctcgac ggtatcggtt aacttttaaa agaaaagggg
     3361 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     3421 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tccgatcacg agactagcct
     3481 cgaggtttat cacaagtttg tacaaaaaag caggcttcac catggccttg gccggggccc
     3541 ctgcgggcgg cccatgcgcg ccggcgctgg aggccctgct cggggccggc gcgctgcggc
     3601 tgctcgactc ctcgcagatc gtcatcatct ccgccgcgca ggacgccagc gccccgccgg
     3661 ctcccaccgg ccccgcggcg cccgccgccg gcccctgcga ccctgacctg ctgctcttcg
     3721 ccacaccgca ggcgccccgg cccacaccca gtgcgccgcg gcccgcgctc ggccgcccgc
     3781 cggtgaagcg gaggctggac ctggaaactg accatcagta cctggccgag agcagtgggc
     3841 cagctcgggg cagaggccgc catccaggaa aaggtgtgaa atccccgggg gagaagtcac
     3901 gctatgagac ctcactgaat ctgaccacca agcgcttcct ggagctgctg agccactcgg
     3961 ctgacggtgt cgtcgacctg aactgggctg ccgaggtgct gaaggtgcag aagcggcgca
     4021 tctatgacat caccaacgtc cttgagggca tccagctcat tgccaagaag tccaagaacc
     4081 acatccagtg gctgggcagc cacaccacag tgggcgtcgg cggacggctt gaggggttga
     4141 cccaggacct ccgacagctg caggagagcg agcagcagct ggaccacctg atgaatatct
     4201 gtactacgca gctgcgcctg ctctccgagg acactgacag ccagcgcctg gcctacgtga
     4261 cgtgtcagga ccttcgtagc attgcagacc ctgcagagca gatggttatg gtgatcaaag
     4321 cccctcctga gacccagctc caagccgtgg actcttcgga gaactttcag atctccctta
     4381 agagcaaaca aggcccgatc gatgttttcc tgtgccctga ggagaccgta ggtgggatca
     4441 gccctgggaa gaccccatcc caggaggtca cttctaagga ggagaacagg gccactgact
     4501 ctgccaccat agtgtcacca ccaccatcat ctcccccctc atccctcacc acagatccca
     4561 gccagtctct actcagcctg gagcaagaac cgctgttgtc ccggatgggc agcctgcggg
     4621 ctcccgtgga cgaggaccgc ctgtccccgc tggtggcggc cgactcgctc ctggagcatg
     4681 tgcgggagga cttctccggc ctcctccctg aggagttcat cagcctttcc ccaccccacg
     4741 aggccctcga ctaccacttc ggcctcgagg agggcgaggg catcagagac ctcttcgact
     4801 gtgactttgg ggacctcacc cccctggatt tctgaaccca gctttcttgt acaaagtggt
     4861 gataaactac gggctgcagg aattccgccc ccccccccct aacgttactg gccgaagccg
     4921 cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt
     4981 tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct
     5041 ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct
     5101 ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc
     5161 acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc
     5221 ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc
     5281 ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc
     5341 tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaacgtcta
     5401 ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata ataccatggt
     5461 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     5521 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     5581 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     5641 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
     5701 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     5761 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     5821 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     5881 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
     5941 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     6001 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     6061 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     6121 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtccggact
     6181 cagatctcga ctagctagta gctagctagc tagtcgagct caacttcgaa ttcgatatca
     6241 agcttatcgc gataccgtcg acctcgaggg aattccgata atcaacctct ggattacaaa
     6301 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     6361 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     6421 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     6481 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     6541 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     6601 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     6661 gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt
     6721 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     6781 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     6841 cggatctccc tttgggccgc ctccccgcat cgggaattcg ctcaagcttc gaattaattc
     6901 tgnagagctc ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     6961 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatggga
     7021 tcaattcacc atgggaataa cttcgtatag catacattat acgaagttat gctgcttttt
     7081 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     7141 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     7201 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     7261 atctctagca gcatctagaa ttaattccgt gtattctata gtgtcaccta aatcgtatgt
     7321 gtatgataca taaggttatg tattaattgt agccgcgttc taacgacaat atgtacaagc
     7381 ctaattgtgt agcatctggc ttactgaagc agaccctatc atctctctcg taaactgccg
     7441 tcagagtcgg tttggttgga cgaaccttct gagtttctgg taacgccgtc ccgcacccgg
     7501 aaatggtcag cgaaccaatc agcagggtca tcgctagcca gatcctctac gccggacgca
     7561 tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca
     7621 ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta
     7681 tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc
     7741 ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta atgcaggagt
     7801 cgcataaggg agagcgtcga atggtgcact ctcagtacaa tctgctctga tgccgcatag
     7861 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     7921 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     7981 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag
     8041 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     8101 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     8161 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     8221 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     8281 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     8341 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     8401 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     8461 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     8521 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     8581 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     8641 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     8701 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     8761 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     8821 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     8881 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     8941 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     9001 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     9061 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     9121 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     9181 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     9241 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     9301 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     9361 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     9421 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     9481 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     9541 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     9601 accgaactga gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga
     9661 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     9721 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     9781 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     9841 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     9901 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     9961 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
    10021 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctgtggaat gtgtgtcagt
    10081 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
    10141 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
    10201 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
    10261 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
    10321 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
    10381 gaggcctagg cttttgcaaa aagcttggac acaagacagg cttgcgagat atgtttgaga
    10441 ataccacttt atcccgcgtc agggagaggc agtgcgtaaa aagacgcgga ctcatgtgaa
    10501 atactggttt ttagtgcgcc agatctctat aatctcgcgc aacctatttt cccctcgaac
    10561 actttttaag ccgtagataa acaggctggg acacttcaca tgagcgaaaa atacatcgtc
    10621 acctgggaca tgttgcagat ccatgcacgt aaactcgcaa gccgactgat gccttctgaa
    10681 caatggaaag gcattattgc cgtaagccgt ggcggtctgt accgggtgcg ttactggcgc
    10741 gtgaactggg tattcgtcat gtcgataccg tttgtatttc cagctacgat cacgacaacc
    10801 agcgcgagct taaagtgctg aaacgcgcag aaggcgatgg cgaaggcttc atcgttattg
    10861 atgacctggt ggataccggt ggtactgcgg ttgcgattcg tgaaatgtat ccaaaagcgc
    10921 actttgtcac catcttcgca aaaccggctg gtcgtccgct ggttgatgac tatgttgttg
    10981 atatcccgca agatacctgg attgaacagc cgtgggatat gggcgtcgta ttcgtcccgc
    11041 caatctccgg tcgctaatct tttcaacgcc tggcactgcc gggcgttgtt ctttttaact
    11101 tcaggcgggt tacaatagtt tccagtaagt attctggagg ctgcatccat gacacaggca
    11161 aacctgagcg aaaccctgtt caaaccccgc tttaaacatc ctgaaacctc gacgctagtc
    11221 cgccgcttta atcacggcgc acaaccgcct gtgcagtcgg cccttgatgg taaaaccatc
    11281 cctcactggt atcgcatgat taaccgtctg atgtggatct ggcgcggcat tgacccacgc
    11341 gaaatcctcg acgtccaggc acgtattgtg atgagcgatg ccgaacgtac cgacgatgat
    11401 ttatacgata cggtgattgg ctaccgtggc ggcaactgga tttatgagtg ggccccggat
    11461 ctttgtgaag gaaccttact tctgtggtgt gacataattg gacaaactac ctacagagat
    11521 ttaaagctct aaggtaaata taaaattttt aagtgtataa tgtgttaaac tactgattct
    11581 aattgtttgt gtattttaga ttccaaccta tggaactgat gaatgggagc agtggtggaa
    11641 tgcctttaat gaggaaaacc tgttttgctc agaagaaatg ccatctagtg atgatgaggc
    11701 tactgctgac tctcaacatt ctactcctcc aaaaaagaag agaaaggtag aagaccccaa
    11761 ggactttcct tcagaattgc taagtttttt gagtcatgct gtgtttagta atagaactct
    11821 tgcttgcttt gctatttaca ccacaaagga aaaagctgca ctgctataca agaaaattat
    11881 ggaaaaatat tctgtaacct ttataagtag gcataacagt tataatcata acatactgtt
    11941 ttttcttact ccacacaggc atagagtgtc tgctattaat aactatgctc aaaaattgtg
    12001 tacctttagc tttttaattt gtaaaggggt taataaggaa tatttgatgt atagtgcctt
    12061 gactagagat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
    12121 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
    12181 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
    12241 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
    12301 tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc
    12361 ctattaccac tgccaattac ctagtggttt catttactct aaacctgtga ttcctctgaa
    12421 ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagt
//
