LOCUS       sequence-215789-       12560 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive expression of the E2F3 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12560)
  AUTHORS   Rady B, Chen Y, Vaca P, Wang Q, Wang Y, Salmon P, Oberholzer J
  TITLE     Overexpression of E2F3 promotes proliferation of functional human 
            beta cells without induction of apoptosis.
  JOURNAL   Cell Cycle. 2013 Aug 15;12(16):2691-702. doi: 10.4161/cc.25834. Epub
            2013 Jul 29.
  PUBMED    23907129
REFERENCE   2  (bases 1 to 12560)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.4161/cc.25834"; 
            journalName: "Cell Cycle"; date: "2013-08-15- 15"; volume: "12"; 
            issue: "16"; pages: "2691-702"
COMMENT     Sequence Label: pWPI-E2F3
FEATURES             Location/Qualifiers
     source          1..12560
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1299..1532
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1717..1761
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2119..3296
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2350..3287
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3244..3264
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3344..3461
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    3492..3516
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4920..4944)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4968..5535
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4986..5537
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5130..5147)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5357..5376
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(5540..5542,5543..5545,5546..6256)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5540..5542,5543..5545,5546..6256)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5585..5606)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5846..5865)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6193..6214
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(6334..6350)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6335..6351)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    6364..6952
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6417..6437)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6835..6846)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(7121..7154)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             7174..7354
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(7379..7397)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7380..7397)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7687..7706)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7920..7939)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8039..8061
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8099..8117)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8185..8289
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8290..8358,8359..9150)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8508..8527)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9321..9909
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9810..9829
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10063..10080
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10150..10170)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10155..10484
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10335..10470
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          11618..11683
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11813..11833
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    12258..12392
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12295..12314)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12349..12368
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     1201 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     1261 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     1321 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     1381 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     1441 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     1501 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     1561 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     1621 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     1681 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     1741 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     1801 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     1861 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     1921 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     1981 gacagatcca ttcgattagt gaacggatct cgacggtatc gatgtcgacg ataagctttg
     2041 caaagatgga taaagtttta aacagagagg aatctttgca gctaatggac cttctaggtc
     2101 ttgaaaggag tgggaattgg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca
     2161 gtccccgaga agttgggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc
     2221 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga
     2281 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc
     2341 agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc
     2401 ccttgcgtgc cttgaattac ttccactggc tgcagtacgt gattcttgat cccgagcttc
     2461 gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg
     2521 cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc
     2581 gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg
     2641 cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta
     2701 tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg
     2761 cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc
     2821 ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc
     2881 tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag
     2941 ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa
     3001 ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc
     3061 cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta ggttgggggg
     3121 aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag
     3181 cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca
     3241 ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgaggaa
     3301 tttcgacatt taaatttaat taatctcgac ggtatcggtt aacttttaaa agaaaagggg
     3361 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     3421 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tccgatcacg agactagcct
     3481 cgaggtttat cacaagtttg tacaaaaaag caggcttcac catgagaaag ggaatccagc
     3541 ccgctctgga gcagtacctg gtgaccgccg ggggtgggga gggggcggct gtcgtcgccg
     3601 ccgccgctgc agcctccatg gacaaaaggg cactgctagc cagccccggc ttcgccgccg
     3661 ccgccgccgc tgccgccgcc ccgggcgcgt acatccagat cctcaccacg aacacttcca
     3721 ccacctcctg ttcctcctcc ctccaaagcg gcgccgtagc cgccggcccc ctcctcccca
     3781 gtgcccccgg cgcggagcag accgccggca gcctcctcta caccacgccg cacggaccct
     3841 ccagcagagc cgggctgctg cagcagccac cagcgctggg acgcggcggc agcggcggcg
     3901 gcggcggccc tccggcaaag cgaaggctgg agctaggaga aagcggtcat cagtacctct
     3961 cagatggttt aaaaaccccc aagggcaaag gaagagctgc actacgaagt ccagatagtc
     4021 caaaaactcc aaaatctccc tcagaaaaaa cgcggtatga tacgtctctt ggtctgctca
     4081 ccaagaagtt cattcagctc ctgagccagt cacccgatgg ggtattggat ttgaacaagg
     4141 cagcagaagt gctaaaagtg caaaagagaa ggatttatga tatcaccaac gttctggaag
     4201 gcatccacct cattaagaag aagtctaaaa acaacgtcca atggatgggc tgcagtctgt
     4261 ctgaggatgg gggcatgctg gcccagtgtc aaggcctgtc aaaagaagtg accgagctca
     4321 gtcaggaaga gaagaaatta gatgaactga tccaaagctg caccctggac ctcaaactgt
     4381 taaccgagga ttcagagaat caaaggttag cttatgttac atatcaagat attcgaaaaa
     4441 ttagtggcct taaagaccaa actgttatag ttgtgaaagc ccctccagaa acaagacttg
     4501 aagtgcctga ctcaatagag agcctacaaa tacatttggc aagtacccaa gggcccattg
     4561 aggtttactt atgtccagaa gagactgaaa cacacagtcc aatgaaaaca aacaaccaag
     4621 accacaatgg gaatatccct aaacccgctt ccaaagactt ggcttcaacc aactcaggac
     4681 atagcgattg ctcagtttct atgggaaacc tttctcctct ggcctcccca gccaacctct
     4741 tacagcagac tgaggaccaa attccttcca acctagaagg accgtttgtg aacttactgc
     4801 ctcccctgct gcaagaggac tatctcctga gcctcgggga ggaggaaggc atcagcgatc
     4861 tcttcgatgc ttacgatttg gaaaagctcc cactggtgga agacttcatg tgtagttgaa
     4921 cccagctttc ttgtacaaag tggtgataaa ctacgggctg caggaattcc gccccccccc
     4981 ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt
     5041 attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt
     5101 cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa
     5161 tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac
     5221 cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg
     5281 tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt
     5341 tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca
     5401 gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt
     5461 ttagtcgagg ttaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga
     5521 aaaacacgat gataatacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     5581 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     5641 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     5701 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
     5761 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     5821 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     5881 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     5941 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     6001 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
     6061 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     6121 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
     6181 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     6241 ggacgagctg tacaagtccg gactcagatc tcgactagct agtagctagc tagctagtcg
     6301 agctcaactt cgaattcgat atcaagctta tcgcgatacc gtcgacctcg agggaattcc
     6361 gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     6421 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     6481 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     6541 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     6601 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     6661 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     6721 ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg
     6781 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     6841 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     6901 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgggaa
     6961 ttcgctcaag cttcgaatta attctgnaga gctcggtacc tttaagacca atgacttaca
     7021 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     7081 actcccaacg aagacaagat gggatcaatt caccatggga ataacttcgt atagcataca
     7141 ttatacgaag ttatgctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     7201 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     7261 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     7321 agaccctttt agtcagtgtg gaaaatctct agcagcatct agaattaatt ccgtgtattc
     7381 tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc
     7441 gttctaacga caatatgtac aagcctaatt gtgtagcatc tggcttactg aagcagaccc
     7501 tatcatctct ctcgtaaact gccgtcagag tcggtttggt tggacgaacc ttctgagttt
     7561 ctggtaacgc cgtcccgcac ccggaaatgg tcagcgaacc aatcagcagg gtcatcgcta
     7621 gccagatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg
     7681 ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca
     7741 tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg
     7801 ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac
     7861 tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgaatggtg cactctcagt
     7921 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
     7981 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     8041 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
     8101 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     8161 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     8221 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     8281 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     8341 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     8401 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     8461 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     8521 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     8581 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     8641 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     8701 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     8761 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     8821 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     8881 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     8941 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     9001 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     9061 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     9121 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     9181 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     9241 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     9301 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     9361 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     9421 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     9481 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     9541 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     9601 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     9661 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gcattgagaa
     9721 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     9781 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     9841 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     9901 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     9961 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
    10021 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
    10081 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
    10141 tgcagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
    10201 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
    10261 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
    10321 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
    10381 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
    10441 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctt ggacacaaga
    10501 caggcttgcg agatatgttt gagaatacca ctttatcccg cgtcagggag aggcagtgcg
    10561 taaaaagacg cggactcatg tgaaatactg gtttttagtg cgccagatct ctataatctc
    10621 gcgcaaccta ttttcccctc gaacactttt taagccgtag ataaacaggc tgggacactt
    10681 cacatgagcg aaaaatacat cgtcacctgg gacatgttgc agatccatgc acgtaaactc
    10741 gcaagccgac tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt
    10801 ctgtaccggg tgcgttactg gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta
    10861 tttccagcta cgatcacgac aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg
    10921 atggcgaagg cttcatcgtt attgatgacc tggtggatac cggtggtact gcggttgcga
    10981 ttcgtgaaat gtatccaaaa gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc
    11041 cgctggttga tgactatgtt gttgatatcc cgcaagatac ctggattgaa cagccgtggg
    11101 atatgggcgt cgtattcgtc ccgccaatct ccggtcgcta atcttttcaa cgcctggcac
    11161 tgccgggcgt tgttcttttt aacttcaggc gggttacaat agtttccagt aagtattctg
    11221 gaggctgcat ccatgacaca ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa
    11281 catcctgaaa cctcgacgct agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag
    11341 tcggcccttg atggtaaaac catccctcac tggtatcgca tgattaaccg tctgatgtgg
    11401 atctggcgcg gcattgaccc acgcgaaatc ctcgacgtcc aggcacgtat tgtgatgagc
    11461 gatgccgaac gtaccgacga tgatttatac gatacggtga ttggctaccg tggcggcaac
    11521 tggatttatg agtgggcccc ggatctttgt gaaggaacct tacttctgtg gtgtgacata
    11581 attggacaaa ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt
    11641 ataatgtgtt aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac
    11701 tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa aacctgtttt gctcagaaga
    11761 aatgccatct agtgatgatg aggctactgc tgactctcaa cattctactc ctccaaaaaa
    11821 gaagagaaag gtagaagacc ccaaggactt tccttcagaa ttgctaagtt ttttgagtca
    11881 tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc
    11941 tgcactgcta tacaagaaaa ttatggaaaa atattctgta acctttataa gtaggcataa
    12001 cagttataat cataacatac tgttttttct tactccacac aggcatagag tgtctgctat
    12061 taataactat gctcaaaaat tgtgtacctt tagcttttta atttgtaaag gggttaataa
    12121 ggaatatttg atgtatagtg ccttgactag agatcataat cagccatacc acatttgtag
    12181 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
    12241 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
    12301 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
    12361 aactcatcaa tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc
    12421 atcccaaact tcccacccca taccctatta ccactgccaa ttacctagtg gtttcattta
    12481 ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata
    12541 tgtactacaa acttagtagt
//
