LOCUS       sequence-215791-       12326 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive expression of the TFDP2 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12326)
  AUTHORS   Rady B, Chen Y, Vaca P, Wang Q, Wang Y, Salmon P, Oberholzer J
  TITLE     Overexpression of E2F3 promotes proliferation of functional human 
            beta cells without induction of apoptosis.
  JOURNAL   Cell Cycle. 2013 Aug 15;12(16):2691-702. doi: 10.4161/cc.25834. Epub
            2013 Jul 29.
  PUBMED    23907129
REFERENCE   2  (bases 1 to 12326)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.4161/cc.25834"; 
            journalName: "Cell Cycle"; date: "2013-08-15- 15"; volume: "12"; 
            issue: "16"; pages: "2691-702"
COMMENT     Sequence Label: pWPI-TFDP2
FEATURES             Location/Qualifiers
     source          1..12326
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1299..1532
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1717..1761
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2119..3296
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2350..3287
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3244..3264
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3344..3461
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    3492..3516
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4686..4710)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4734..5301
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4752..5303
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4896..4913)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5123..5142
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(5306..5308,5309..5311,5312..6022)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5306..5308,5309..5311,5312..6022)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5351..5372)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5612..5631)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5959..5980
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(6100..6116)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6101..6117)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    6130..6718
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6183..6203)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6601..6612)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(6887..6920)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             6940..7120
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(7145..7163)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7146..7163)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7453..7472)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7686..7705)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7805..7827
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7865..7883)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7951..8055
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8056..8124,8125..8916)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8274..8293)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9087..9675
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9576..9595
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9829..9846
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9916..9936)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9921..10250
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10101..10236
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          11384..11449
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11579..11599
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    12024..12158
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12061..12080)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12115..12134
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     1201 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     1261 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     1321 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     1381 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     1441 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     1501 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     1561 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     1621 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     1681 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     1741 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     1801 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     1861 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     1921 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     1981 gacagatcca ttcgattagt gaacggatct cgacggtatc gatgtcgacg ataagctttg
     2041 caaagatgga taaagtttta aacagagagg aatctttgca gctaatggac cttctaggtc
     2101 ttgaaaggag tgggaattgg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca
     2161 gtccccgaga agttgggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc
     2221 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga
     2281 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc
     2341 agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc
     2401 ccttgcgtgc cttgaattac ttccactggc tgcagtacgt gattcttgat cccgagcttc
     2461 gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg
     2521 cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc
     2581 gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg
     2641 cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta
     2701 tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg
     2761 cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc
     2821 ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc
     2881 tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag
     2941 ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa
     3001 ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc
     3061 cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta ggttgggggg
     3121 aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag
     3181 cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca
     3241 ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgaggaa
     3301 tttcgacatt taaatttaat taatctcgac ggtatcggtt aacttttaaa agaaaagggg
     3361 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     3421 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tccgatcacg agactagcct
     3481 cgaggtttat cacaagtttg tacaaaaaag caggcttcac catgattata agcacaccac
     3541 agagactaac cagttcagga agtgttctga ttgggagtcc atatacccct gcaccagcaa
     3601 tggttactca gacacacata gcagaagcta ctggctgggt ccctggtgat agaaaacggg
     3661 ctagaaaatt tatagactct gatttttcag aaagtaaacg aagcaaaaaa ggagataaaa
     3721 atgggaaagg cttgagacac ttttcaatga aagtgtgtga gaaagttcaa cgaaaaggta
     3781 caacatcgta caatgaagtc gctgatgagc tggtgtcaga gttcaccaat tcaaataacc
     3841 atttggctgc tgattcgcag gcttatgatc agaagaacat taggcgaaga gtttatgatg
     3901 ctttaaatgt gctaatggca atgaacataa tttcaaagga aaaaaaagaa atcaagtgga
     3961 ttggcctgcc taccaattct gctcaggaat gtcagaatct ggagatagag aagcagaggc
     4021 ggatagaacg gataaagcag aagcgggccc agctgcaaga acttctccta cagcaaatcg
     4081 ctttcaaaaa cctggtacag agaaatcgac aaaatgagca gcaaaaccag ggcccgccgg
     4141 ctctgaactc taccattcag ctgccattca taatcatcaa tacaagcaga aaaacagtca
     4201 tagattgcag catctccagt gacaagtttg agtatctttt caattttgac aacacctttg
     4261 agatccatga tgacatagaa gtactaaagc ggatgggaat gtcgtttggc ctggagtcag
     4321 gcaaatgctc tctggaggat ctgaaacttg cgaaatccct ggtgccaaag gctttagaag
     4381 gttatatcac agatatctcc acaggacctt cttggttaaa tcagggacta cttctgaact
     4441 ctacccaatc agtttcaaat ttagacctga ccactggtgc caccttaccc cagtcaagtg
     4501 taaaccaagg gttatgcttg gatgcagaag tggccttagc aactgggcag ttcctggccc
     4561 caaacagtca ccagtccagc agtgcggcct ctcactgctc cgagtcccga ggcgagaccc
     4621 cctgttcgtt caatgatgaa gatgaggaag atgatgagga ggattcctcc tccccagaat
     4681 aatgaaccca gctttcttgt acaaagtggt gataaactac gggctgcagg aattccgccc
     4741 ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta
     4801 tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc
     4861 tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct
     4921 gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt
     4981 agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa
     5041 gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg
     5101 gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga
     5161 tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac
     5221 atgtgtttag tcgaggttaa aaaacgtcta ggccccccga accacgggga cgtggttttc
     5281 ctttgaaaaa cacgatgata ataccatggt gagcaagggc gaggagctgt tcaccggggt
     5341 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     5401 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     5461 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt
     5521 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     5581 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     5641 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     5701 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     5761 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
     5821 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     5881 ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc
     5941 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     6001 cggcatggac gagctgtaca agtccggact cagatctcga ctagctagta gctagctagc
     6061 tagtcgagct caacttcgaa ttcgatatca agcttatcgc gataccgtcg acctcgaggg
     6121 aattccgata atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     6181 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     6241 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     6301 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     6361 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     6421 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     6481 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
     6541 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     6601 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     6661 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat
     6721 cgggaattcg ctcaagcttc gaattaattc tgnagagctc ggtaccttta agaccaatga
     6781 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     6841 taattcactc ccaacgaaga caagatggga tcaattcacc atgggaataa cttcgtatag
     6901 catacattat acgaagttat gctgcttttt gcttgtactg ggtctctctg gttagaccag
     6961 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     7021 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     7081 tccctcagac ccttttagtc agtgtggaaa atctctagca gcatctagaa ttaattccgt
     7141 gtattctata gtgtcaccta aatcgtatgt gtatgataca taaggttatg tattaattgt
     7201 agccgcgttc taacgacaat atgtacaagc ctaattgtgt agcatctggc ttactgaagc
     7261 agaccctatc atctctctcg taaactgccg tcagagtcgg tttggttgga cgaaccttct
     7321 gagtttctgg taacgccgtc ccgcacccgg aaatggtcag cgaaccaatc agcagggtca
     7381 tcgctagcca gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg
     7441 cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg
     7501 ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt
     7561 tgggcgccat ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc
     7621 tactactggg ctgcttccta atgcaggagt cgcataaggg agagcgtcga atggtgcact
     7681 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
     7741 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     7801 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
     7861 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     7921 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     7981 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     8041 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     8101 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     8161 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     8221 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     8281 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     8341 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     8401 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     8461 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     8521 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     8581 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     8641 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     8701 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     8761 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     8821 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     8881 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     8941 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     9001 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     9061 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     9121 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     9181 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     9241 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     9301 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     9361 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     9421 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcat
     9481 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     9541 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     9601 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     9661 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     9721 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     9781 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     9841 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     9901 cattaatgca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     9961 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
    10021 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
    10081 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
    10141 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
    10201 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagcttggac
    10261 acaagacagg cttgcgagat atgtttgaga ataccacttt atcccgcgtc agggagaggc
    10321 agtgcgtaaa aagacgcgga ctcatgtgaa atactggttt ttagtgcgcc agatctctat
    10381 aatctcgcgc aacctatttt cccctcgaac actttttaag ccgtagataa acaggctggg
    10441 acacttcaca tgagcgaaaa atacatcgtc acctgggaca tgttgcagat ccatgcacgt
    10501 aaactcgcaa gccgactgat gccttctgaa caatggaaag gcattattgc cgtaagccgt
    10561 ggcggtctgt accgggtgcg ttactggcgc gtgaactggg tattcgtcat gtcgataccg
    10621 tttgtatttc cagctacgat cacgacaacc agcgcgagct taaagtgctg aaacgcgcag
    10681 aaggcgatgg cgaaggcttc atcgttattg atgacctggt ggataccggt ggtactgcgg
    10741 ttgcgattcg tgaaatgtat ccaaaagcgc actttgtcac catcttcgca aaaccggctg
    10801 gtcgtccgct ggttgatgac tatgttgttg atatcccgca agatacctgg attgaacagc
    10861 cgtgggatat gggcgtcgta ttcgtcccgc caatctccgg tcgctaatct tttcaacgcc
    10921 tggcactgcc gggcgttgtt ctttttaact tcaggcgggt tacaatagtt tccagtaagt
    10981 attctggagg ctgcatccat gacacaggca aacctgagcg aaaccctgtt caaaccccgc
    11041 tttaaacatc ctgaaacctc gacgctagtc cgccgcttta atcacggcgc acaaccgcct
    11101 gtgcagtcgg cccttgatgg taaaaccatc cctcactggt atcgcatgat taaccgtctg
    11161 atgtggatct ggcgcggcat tgacccacgc gaaatcctcg acgtccaggc acgtattgtg
    11221 atgagcgatg ccgaacgtac cgacgatgat ttatacgata cggtgattgg ctaccgtggc
    11281 ggcaactgga tttatgagtg ggccccggat ctttgtgaag gaaccttact tctgtggtgt
    11341 gacataattg gacaaactac ctacagagat ttaaagctct aaggtaaata taaaattttt
    11401 aagtgtataa tgtgttaaac tactgattct aattgtttgt gtattttaga ttccaaccta
    11461 tggaactgat gaatgggagc agtggtggaa tgcctttaat gaggaaaacc tgttttgctc
    11521 agaagaaatg ccatctagtg atgatgaggc tactgctgac tctcaacatt ctactcctcc
    11581 aaaaaagaag agaaaggtag aagaccccaa ggactttcct tcagaattgc taagtttttt
    11641 gagtcatgct gtgtttagta atagaactct tgcttgcttt gctatttaca ccacaaagga
    11701 aaaagctgca ctgctataca agaaaattat ggaaaaatat tctgtaacct ttataagtag
    11761 gcataacagt tataatcata acatactgtt ttttcttact ccacacaggc atagagtgtc
    11821 tgctattaat aactatgctc aaaaattgtg tacctttagc tttttaattt gtaaaggggt
    11881 taataaggaa tatttgatgt atagtgcctt gactagagat cataatcagc cataccacat
    11941 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
    12001 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
    12061 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
    12121 tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc
    12181 aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac ctagtggttt
    12241 catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt
    12301 taaatatgta ctacaaactt agtagt
//
