LOCUS       sequence-215797-       12320 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive expression of the Pax4 trancription factor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12320)
  TITLE     Salmon Lab plasmids
REFERENCE   2  (bases 1 to 12320)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pWPI-mPAX4
FEATURES             Location/Qualifiers
     source          1..12320
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             2..635
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    682..807
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1300..1533
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1718..1762
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2120..3297
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2351..3288
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3245..3265
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3345..3462
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             4639..4668
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4684..4701
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    4729..5296
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4747..5298
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4891..4908)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5118..5137
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(5301..5303,5304..5306,5307..6017)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5301..5303,5304..5306,5307..6017)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5346..5367)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5607..5626)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5954..5975
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(6095..6111)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6096..6112)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    6125..6713
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6178..6198)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6596..6607)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(6882..6915)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             6935..7115
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(7140..7158)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7141..7158)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7448..7467)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7681..7700)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7800..7822
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7860..7878)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7946..8050
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8051..8119,8120..8911)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8269..8288)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9082..9670
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9571..9590
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9824..9841
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9911..9931)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9916..10245
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10096..10231
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          11379..11444
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11574..11594
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    12019..12153
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12056..12075)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12110..12129
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
       61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
      121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
      181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
      241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
      301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
      361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
      421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
      481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
      961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1141 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     1981 agacagatcc attcgattag tgaacggatc tcgacggtat cgatgtcgac gataagcttt
     2041 gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt
     2101 cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac
     2161 agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg
     2221 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg
     2281 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc
     2341 cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg
     2401 cccttgcgtg ccttgaatta cttccactgg ctgcagtacg tgattcttga tcccgagctt
     2461 cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt
     2521 gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt
     2581 cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct
     2641 gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt
     2701 atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg
     2761 gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc
     2821 cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg
     2881 ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca
     2941 gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa
     3001 aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg
     3061 ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg
     3121 gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca
     3181 gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc
     3241 attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgagga
     3301 atttcgacat ttaaatttaa ttaatctcga cggtatcggt taacttttaa aagaaaaggg
     3361 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     3421 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttccgatcac gagactagcc
     3481 tcgaggtttg atccactagt ccagtgtggt ggaattcgat ttgaagcatg cagcaggacg
     3541 gactcagcag tgtgaatcag ctagggggac tctttgtgaa tggccggccc cttcctctgg
     3601 acaccaggca gcagattgtg cagctagcaa taagagggat gcgaccctgt gacatttcac
     3661 ggagccttaa ggtatctaat ggctgtgtga gcaagatcct aggacgctac taccgcacag
     3721 gtgtcttgga acccaagtgt attgggggaa gcaaaccacg tctggccaca cctgctgtgg
     3781 tggctcgaat tgcccagcta aaggatgagt accctgctct ttttgcctgg gagatccaac
     3841 accagctttg cactgaaggg ctttgtaccc aggacaaggc tcccagtgtg tcctctatca
     3901 atcgagtact tcgggcactt caggaagacc agagcttgca ctggactcaa ctcagatcac
     3961 cagctgtgtt ggctccagtt cttcccagtc cccacagtaa ctgtggggct ccccgaggtc
     4021 cccacccagg aaccagccac aggaatcgga ctatcttctc cccgggacaa gccgaggcac
     4081 tggagaaaga gtttcagcgt gggcagtatc cagattcagt ggcccgtggg aagctggctg
     4141 ctgccacctc tctgcctgaa gacacggtga gggtttggtt ttctaacaga agagccaaat
     4201 ggcgcaggca agagaagctg aaatgggaag cacagctgcc aggtgcttcc caggacctga
     4261 cagtaccaaa aaattctcca gggatcatct ctgcacagca gtcccccggc agtgtaccct
     4321 cagctgcctt gcctgtgctg gaaccattga gtccttcctt ctgtcagcta tgctgtggga
     4381 cagcaccagg cagatgttcc agtgacacct catcccaggc ctatctccaa ccctactggg
     4441 actgccaatc cctccttcct gtggcttcct cctcatatgt ggaatttgcc tggccctgcc
     4501 tcaccaccca tcctgtgcat catctgattg gaggcccagg acaagtgcca tcaacccatt
     4561 gctcaaactg gccaatccac tagtgaattc tgcagatatc cagcacagtg gcggccgctc
     4621 gagtctagag ggcccttcga acaaaaactc atctcagaag aggatctgaa tatgcatacc
     4681 ggtcatcatc accatcacca ttgagtttaa actacgggct gcaggaattc cgcccccccc
     4741 cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt
     4801 tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct
     4861 tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga
     4921 atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga
     4981 ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac
     5041 gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag
     5101 ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc
     5161 agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg
     5221 tttagtcgag gttaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg
     5281 aaaaacacga tgataatacc atggtgagca agggcgagga gctgttcacc ggggtggtgc
     5341 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     5401 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     5461 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc
     5521 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     5581 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     5641 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     5701 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
     5761 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
     5821 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     5881 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
     5941 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     6001 tggacgagct gtacaagtcc ggactcagat ctcgactagc tagtagctag ctagctagtc
     6061 gagctcaact tcgaattcga tatcaagctt atcgcgatac cgtcgacctc gagggaattc
     6121 cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     6181 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     6241 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     6301 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     6361 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     6421 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     6481 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct
     6541 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     6601 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     6661 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcggga
     6721 attcgctcaa gcttcgaatt aattctgcag agctcggtac ctttaagacc aatgacttac
     6781 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     6841 cactcccaac gaagacaaga tgggatcaat tcaccatggg aataacttcg tatagcatac
     6901 attatacgaa gttatgctgc tttttgcttg tactgggtct ctctggttag accagatctg
     6961 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     7021 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     7081 cagacccttt tagtcagtgt ggaaaatctc tagcagcatc tagaattaat tccgtgtatt
     7141 ctatagtgtc acctaaatcg tatgtgtatg atacataagg ttatgtatta attgtagccg
     7201 cgttctaacg acaatatgta caagcctaat tgtgtagcat ctggcttact gaagcagacc
     7261 ctatcatctc tctcgtaaac tgccgtcaga gtcggtttgg ttggacgaac cttctgagtt
     7321 tctggtaacg ccgtcccgca cccggaaatg gtcagcgaac caatcagcag ggtcatcgct
     7381 agccagatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt
     7441 gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc
     7501 atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc
     7561 gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta
     7621 ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaatggt gcactctcag
     7681 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
     7741 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     7801 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
     7861 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     7921 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     7981 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     8041 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     8101 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     8161 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     8221 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     8281 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     8341 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     8401 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     8461 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     8521 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     8581 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     8641 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     8701 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     8761 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     8821 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     8881 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     8941 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     9001 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     9061 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     9121 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     9181 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     9241 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     9301 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     9361 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     9421 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agcattgaga
     9481 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     9541 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     9601 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     9661 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     9721 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     9781 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     9841 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     9901 atgcagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     9961 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
    10021 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
    10081 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
    10141 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
    10201 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct tggacacaag
    10261 acaggcttgc gagatatgtt tgagaatacc actttatccc gcgtcaggga gaggcagtgc
    10321 gtaaaaagac gcggactcat gtgaaatact ggtttttagt gcgccagatc tctataatct
    10381 cgcgcaacct attttcccct cgaacacttt ttaagccgta gataaacagg ctgggacact
    10441 tcacatgagc gaaaaataca tcgtcacctg ggacatgttg cagatccatg cacgtaaact
    10501 cgcaagccga ctgatgcctt ctgaacaatg gaaaggcatt attgccgtaa gccgtggcgg
    10561 tctgtaccgg gtgcgttact ggcgcgtgaa ctgggtattc gtcatgtcga taccgtttgt
    10621 atttccagct acgatcacga caaccagcgc gagcttaaag tgctgaaacg cgcagaaggc
    10681 gatggcgaag gcttcatcgt tattgatgac ctggtggata ccggtggtac tgcggttgcg
    10741 attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc ggctggtcgt
    10801 ccgctggttg atgactatgt tgttgatatc ccgcaagata cctggattga acagccgtgg
    10861 gatatgggcg tcgtattcgt cccgccaatc tccggtcgct aatcttttca acgcctggca
    10921 ctgccgggcg ttgttctttt taacttcagg cgggttacaa tagtttccag taagtattct
    10981 ggaggctgca tccatgacac aggcaaacct gagcgaaacc ctgttcaaac cccgctttaa
    11041 acatcctgaa acctcgacgc tagtccgccg ctttaatcac ggcgcacaac cgcctgtgca
    11101 gtcggccctt gatggtaaaa ccatccctca ctggtatcgc atgattaacc gtctgatgtg
    11161 gatctggcgc ggcattgacc cacgcgaaat cctcgacgtc caggcacgta ttgtgatgag
    11221 cgatgccgaa cgtaccgacg atgatttata cgatacggtg attggctacc gtggcggcaa
    11281 ctggatttat gagtgggccc cggatctttg tgaaggaacc ttacttctgt ggtgtgacat
    11341 aattggacaa actacctaca gagatttaaa gctctaaggt aaatataaaa tttttaagtg
    11401 tataatgtgt taaactactg attctaattg tttgtgtatt ttagattcca acctatggaa
    11461 ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga aaacctgttt tgctcagaag
    11521 aaatgccatc tagtgatgat gaggctactg ctgactctca acattctact cctccaaaaa
    11581 agaagagaaa ggtagaagac cccaaggact ttccttcaga attgctaagt tttttgagtc
    11641 atgctgtgtt tagtaataga actcttgctt gctttgctat ttacaccaca aaggaaaaag
    11701 ctgcactgct atacaagaaa attatggaaa aatattctgt aacctttata agtaggcata
    11761 acagttataa tcataacata ctgttttttc ttactccaca caggcataga gtgtctgcta
    11821 ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa ggggttaata
    11881 aggaatattt gatgtatagt gccttgacta gagatcataa tcagccatac cacatttgta
    11941 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
    12001 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
    12061 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
    12121 aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat
    12181 catcccaaac ttcccacccc ataccctatt accactgcca attacctagt ggtttcattt
    12241 actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat
    12301 atgtactaca aacttagtag
//
