LOCUS       sequence-215798-        9914 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TET-inducible expression of the Neugenin 3 transcription factor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9914)
  AUTHORS   Giry-Laterriere M, Cherpin O, Kim YS, Jensen J, Salmon P
  TITLE     Polyswitch lentivectors: ""all-in-one"" lentiviral vectors for 
            drug-inducible gene expression, live selection, and recombination 
            cloning.
  JOURNAL   Hum Gene Ther. 2011 Oct;22(10):1255-67. Epub 2011 Sep 27.
  PUBMED    21761975
REFERENCE   2  (bases 1 to 9914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Gene 
            Ther."; date: "2011-10"; volume: "22(10)"; pages: "1255-67. Epub 
            2011 Sep 27"
COMMENT     Sequence Label: pCLXEBR-pTF-Ngn3
FEATURES             Location/Qualifiers
     source          1..9914
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             385..642
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    689..814
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1306..1423
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    1783..2016
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2201..2245
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     protein_bind    2536..2554
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    2577..2595
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    2741..2759
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    2844..2868
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3517..3541)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     3578..3594
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     3579..3595
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    3610..3727
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3783..3994
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             4013..4408
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             4418..4477
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             5540..5911
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     misc_feature    5937..6525
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5990..6010)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6408..6419)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(6667..6700)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             6720..6900
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6938..6957)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7003..7058
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7057..7076)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7417..7433)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7417..7433)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7430..7452)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7441..7457
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7465..7471,7472..7489,7490..7495))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7510..7531
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7590..7785
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7636..7771
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7791..7925
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7828..7847)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7882..7901
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7993..8010)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8164..8752)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8244..8263)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8923..9714,9715..9783))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9546..9565
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9784..9888)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggaggcg tggcctgggc gggactgggg agtggcgagc
      421 cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc
      481 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
      541 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
      601 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
      661 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
      721 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
      781 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
      841 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
      901 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
      961 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     1021 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     1081 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaaaaa
     1141 agcacagcaa gcagcaggat cttcagacct ggaaattccc tacaatcccc aaagtcaagg
     1201 agtagtagaa tctatgaata aagaattaaa gaaaattata ggacaggtaa gagatcaggc
     1261 tgaacatctt aagacagcag tacaaatggc agtattcatc cacaatttta aaagaaaagg
     1321 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     1381 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     1441 cagcagaaat ccactttgga aaggaccagc aaagctcctc tggaaaggtg aaggggcagt
     1501 agtaatacaa gataatagtg acataaaagt agtgccaaga agaaaagcaa agatcattag
     1561 ggattatgga aaacagatgg caggtgatga ttgtgtggca agtagacagg atgaggatta
     1621 gaacatggaa aagtttagta aaacaccata aggaggagat atgagggaca attggagaag
     1681 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1741 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1801 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1861 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1921 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1981 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     2041 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     2101 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     2161 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     2221 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     2281 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     2341 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     2401 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     2461 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttcg gtacaactcc
     2521 cactagacat atcctactct atcattgata gagtgaccct tccatagaca tatcctactc
     2581 tatcattgat agagtgaccc ttccactagg ttcacttttc tctatcattg atagggagtg
     2641 gtaaacaatt gatggacaaa atctatcaat gatagagagt gcatgttact agtattgttc
     2701 ttctctatca ttgatagtga ctttgcctaa tcacttagca actctatcat tgatagagtt
     2761 ctagtattag caaggccttt cctctatata aggaagttca tttcatttgg agaagatccc
     2821 cggggatcct ctagagtcga atcacaagtt tgtacaaaaa agcaggcacc atgacgcctc
     2881 aaccctcggg tgcgcccact gtccaagtga cccgtgagac ggagcggtcc ttccccagag
     2941 cctcggaaga cgaagtgacc tgccccacgt ccgccccgcc cagccccact cgcacacggg
     3001 ggaactgcgc agaggcggaa gagggaggct gccgaggggc cccgaggaag ctccgggcac
     3061 ggcgcggggg acgcagccgg cctaagagcg agttggcact gagcaagcag cgacggagtc
     3121 ggcgaaagaa ggccaacgac cgcgagcgca atcgaatgca caacctcaac tcggcactgg
     3181 acgccctgcg cggtgtcctg cccaccttcc cagacgacgc gaagctcacc aagatcgaga
     3241 cgctgcgctt cgcccacaac tacatctggg cgctgactca aacgctgcgc atagcggacc
     3301 acagcttgta cgcgctggag ccgccggcgc cgcactgcgg ggagctgggc agcccaggcg
     3361 gttcccccgg ggactggggg tccctctact ccccagtctc ccaggctggc agcctgagtc
     3421 ccgccgcgtc gctggaggag cgacccgggc tgctgggggc cacctcttcc gcctgcttga
     3481 gcccaggcag tctggctttc tcagattttc tgtagaaccc agctttcttg tacaaagtgg
     3541 tgattcgacc tgcaggcatg caagctcggg ccccccctcg aggtcgacgg tatcgataag
     3601 cttgataact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     3661 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
     3721 aaattttccg atcacgagac tagcctcgag aagcttgatc gatggctccg gtgcccgtca
     3781 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
     3841 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
     3901 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
     3961 tctttttcgc aacgggtttg ccgccagaac acaggtgtcg tgacgcggat ccatggccaa
     4021 gcctttgtct caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat
     4081 ccccatctct gaagactaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt
     4141 cactggtgtc aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg
     4201 cactgctgct gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa
     4261 caggggcatc ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg
     4321 gatcaaagcc atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt
     4381 gctgccctct ggttatgtgt gggagggcgg atcaggtcaa tgtactaact acgctttgtt
     4441 gaagcttgcc ggcgatgttg aaagtaaccc cggtcctatg tccagactgg acaagagcaa
     4501 agtcataaac ggcgctctgg aattactcaa tggagtcggt atcgaaggcc tgacgacaag
     4561 gaaactcgct caaaagctgg gagttgagca gcctaccctg tactggcacg tgaagaacaa
     4621 gcgggccctg ctcgatgccc tgccaatcga gatgctggac aggcatcata cccacttctg
     4681 ccccctggaa ggcgagtcat ggcaagactt tctgcggaac aacgccaagt cattccgctg
     4741 tgctctcctc tcacatcgcg acggggctaa agtgcatctc ggcacccgcc caacagagaa
     4801 acagtacgaa accctggaaa atcagctcgc gttcctgtgt cagcaaggct tctccctgga
     4861 gaacgcactg tacgctctgt ccgccgtggg ccactttaca ctgggctgcg tattggagga
     4921 acaggagcat caagtagcaa aagaggaaag agagacacct accaccgatt ctatgccccc
     4981 acttctgaga caagcaattg agctgttcga ccggcaggga gccgaacctg ccttcctttt
     5041 cggcctggaa ctaatcatat gtggcctgga gaaacagcta aagtgcgaaa gcggcgggcc
     5101 ggcctcgagc gaattccagt acctgccaga tacagacgat cgtcaccgga ttgaggagaa
     5161 acgtaaaagg acatatgaga ccttcaagag catcatgaag aagagtcctt tcagcggacc
     5221 caccgacccc cggcctccac ctcgacgcat tgctgtgcct tcccgcagct cagcttctgt
     5281 ccccaagcca gcaccccagc cctatccctt tacgtcatcc ctgagcacca tcaactatga
     5341 tgagtttccc accatggtgt ttccttctgg gcagatcagc caggcctcgg ccttggcccc
     5401 ggcccctccc caagtcctgc cccaggctcc agcccctgcc cctgctccag ccatggtatc
     5461 agctctggcc caggccccag cccctgtccc agtcctagcc ccaggccctc ctcaggctgt
     5521 ggccccacct gcccccaagc ccacccaggc tggggaagga acgctgtcag aggccctgct
     5581 gcagctgcag tttgatgatg aagacctggg ggccttgctt ggcaacagca cagacccagc
     5641 tgtgttcaca gacctggcat ccgtcgacaa ctccgagttt cagcagctgc tgaaccaggg
     5701 catacctgtg gccccccaca caactgagcc catgctgatg gagtaccctg aggctataac
     5761 tcgcctagtg acaggggccc agaggccccc cgacccagct cctgctccac tgggggcccc
     5821 ggggctcccc aatggcctcc tttcaggaga tgaagacttc tcctccattg cggacatgga
     5881 cttctcagcc ctgctgagtc agatcagctc ctaaagatcc gcgtcgtacg gtcgacaatc
     5941 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     6001 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     6061 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     6121 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     6181 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     6241 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     6301 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct
     6361 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     6421 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     6481 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga attcgagctc
     6541 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     6601 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatggga tcaattcacc
     6661 atgggaataa cttcgtatag catacattat acgaagttat gctgcttttt gcttgtactg
     6721 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     6781 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     6841 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     6901 gcatctagtt gaatcgccag tgtgtcgagc tagcagatct ttttccctct gccaaaaatt
     6961 atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt
     7021 tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg
     7081 caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgccatat
     7141 gctggctgcc atgaacaaag gtggctataa agaggtcatc agtatatgaa acagccccct
     7201 gctgtccatt ccttattcca tagaaaagcc ttgacttgag gttagatttt ttttatattt
     7261 tgttttgtgt tatttttttc tttaacatcc ctaaaatttt ccttacatgt tttactagcc
     7321 agatttttcc tcctctcctg actactccca gtcatagctg tccctcttct cttatgaaga
     7381 tccctcgacc tgcagcccaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     7441 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     7501 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     7561 gtcgggaaac ctgtcgtgcc agcggatccg catctcaatt agtcagcaac catagtcccg
     7621 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     7681 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     7741 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct aacttgttta
     7801 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     7861 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     7921 ggatccgctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     7981 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     8041 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     8101 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     8161 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     8221 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     8281 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     8341 aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     8401 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     8461 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     8521 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     8581 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     8641 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     8701 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     8761 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     8821 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     8881 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     8941 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     9001 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     9061 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     9121 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     9181 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     9241 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     9301 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     9361 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     9421 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     9481 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     9541 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     9601 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     9661 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     9721 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     9781 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     9841 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     9901 aaaagtgcca cctg
//
