LOCUS       sequence-215799-       10335 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TET-inducible expression of the Neugenin 3 transcription factor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10335)
  TITLE     Salmon Lab plasmids
REFERENCE   2  (bases 1 to 10335)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCWXPGR-pTF-Ngn3
FEATURES             Location/Qualifiers
     source          1..10335
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             385..642
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    689..814
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1306..1423
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    1783..2016
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2201..2245
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     protein_bind    2522..2542
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    2569..2587
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    2610..2628
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    2774..2792
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    2854..2878
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3527..3551)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        3569..4079
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3998..4016
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      4095..4104
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4101..4103,4104..4106,4107..4817)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4101..4103,4104..4106,4107..4817)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4146..4167)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4407..4426)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4754..4775
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4833..4892
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             5961..6332
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     misc_feature    6352..6940
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6405..6425)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6823..6834)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6943..6959)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6944..6960)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    complement(7088..7121)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             7141..7321
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7359..7378)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7424..7479
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7478..7497)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7838..7854)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7838..7854)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7851..7873)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7862..7878
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7886..7892,7893..7910,7911..7916))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7931..7952
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8011..8206
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8057..8192
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    8212..8346
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8249..8268)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8303..8322
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8414..8431)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8585..9173)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8665..8684)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9344..10135,10136..10204))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9967..9986
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10205..10309)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggaggcg tggcctgggc gggactgggg agtggcgagc
      421 cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc
      481 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
      541 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
      601 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
      661 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
      721 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
      781 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
      841 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
      901 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
      961 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     1021 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     1081 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaaaaa
     1141 agcacagcaa gcagcaggat cttcagacct ggaaattccc tacaatcccc aaagtcaagg
     1201 agtagtagaa tctatgaata aagaattaaa gaaaattata ggacaggtaa gagatcaggc
     1261 tgaacatctt aagacagcag tacaaatggc agtattcatc cacaatttta aaagaaaagg
     1321 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     1381 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     1441 cagcagaaat ccactttgga aaggaccagc aaagctcctc tggaaaggtg aaggggcagt
     1501 agtaatacaa gataatagtg acataaaagt agtgccaaga agaaaagcaa agatcattag
     1561 ggattatgga aaacagatgg caggtgatga ttgtgtggca agtagacagg atgaggatta
     1621 gaacatggaa aagtttagta aaacaccata aggaggagat atgagggaca attggagaag
     1681 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1741 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1801 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1861 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1921 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1981 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     2041 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     2101 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     2161 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     2221 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     2281 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     2341 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     2401 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     2461 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgctagt gattatcgga
     2521 tcaactttgt atagaaaagt tgggtacaac tcccactaga catatcctac tctatcattg
     2581 atagagtgac ccttccatag acatatccta ctctatcatt gatagagtga cccttccact
     2641 aggttcactt ttctctatca ttgataggga gtggtaaaca attgatggac aaaatctatc
     2701 aatgatagag agtgcatgtt actagtattg ttcttctcta tcattgatag tgactttgcc
     2761 taatcactta gcaactctat cattgataga gttctagtat tagcaaggcc tttcctctat
     2821 ataaggaagt tcatttcatt tggagaagat cccccaagtt tgtacaaaaa agcaggcacc
     2881 atgacgcctc aaccctcggg tgcgcccact gtccaagtga cccgtgagac ggagcggtcc
     2941 ttccccagag cctcggaaga cgaagtgacc tgccccacgt ccgccccgcc cagccccact
     3001 cgcacacggg ggaactgcgc agaggcggaa gagggaggct gccgaggggc cccgaggaag
     3061 ctccgggcac ggcgcggggg acgcagccgg cctaagagcg agttggcact gagcaagcag
     3121 cgacggagtc ggcgaaagaa ggccaacgac cgcgagcgca atcgaatgca caacctcaac
     3181 tcggcactgg acgccctgcg cggtgtcctg cccaccttcc cagacgacgc gaagctcacc
     3241 aagatcgaga cgctgcgctt cgcccacaac tacatctggg cgctgactca aacgctgcgc
     3301 atagcggacc acagcttgta cgcgctggag ccgccggcgc cgcactgcgg ggagctgggc
     3361 agcccaggcg gttcccccgg ggactggggg tccctctact ccccagtctc ccaggctggc
     3421 agcctgagtc ccgccgcgtc gctggaggag cgacccgggc tgctgggggc cacctcttcc
     3481 gcctgcttga gcccaggcag tctggctttc tcagattttc tgtagaaccc agctttcttg
     3541 tacaaagtgg tgataatcga attcccacgg ggttggggtt gcgccttttc caaggcagcc
     3601 ctgggtttgc gcagggacgc ggctgctctg ggcgtggttc cgggaaacgc agcggcgccg
     3661 accctgggtc tcgcacattc ttcacgtccg ttcgcagcgt cacccggatc ttcgccgcta
     3721 cccttgtggg ccccccggcg acgcttcctg ctccgcccct aagtcgggaa ggttccttgc
     3781 ggttcgcggc gtgccggacg tgacaaacgg aagccgcacg tctcactagt accctcgcag
     3841 acggacagcg ccagggagca atggcagcgc gccgaccgcg atgggctgtg gccaatagcg
     3901 gctgctcagc ggggcgcgcc gagagcagcg gccgggaagg ggcggtgcgg gaggcggggt
     3961 gtggggcggt agtgtgggcc ctgttcctgc ccgcgcggtg ttccgcattc tgcaagcctc
     4021 cggagcgcac gtcggcagtc ggctccctcg ttgaccgaat caccgacctc tctccccagg
     4081 gggatccacc ggtcgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc
     4141 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     4201 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     4261 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc
     4321 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     4381 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     4441 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     4501 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
     4561 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
     4621 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     4681 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
     4741 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     4801 tggacgagct gtacaaggct agcggatcag gtcaatgtac taactacgct ttgttgaagc
     4861 ttgccggcga tgttgaaagt aaccccggtc ctggatccat gtccagactg gacaagagca
     4921 aagtcataaa cggcgctctg gaattactca atggagtcgg tatcgaaggc ctgacgacaa
     4981 ggaaactcgc tcaaaagctg ggagttgagc agcctaccct gtactggcac gtgaagaaca
     5041 agcgggccct gctcgatgcc ctgccaatcg agatgctgga caggcatcat acccacttct
     5101 gccccctgga aggcgagtca tggcaagact ttctgcggaa caacgccaag tcattccgct
     5161 gtgctctcct ctcacatcgc gacggggcta aagtgcatct cggcacccgc ccaacagaga
     5221 aacagtacga aaccctggaa aatcagctcg cgttcctgtg tcagcaaggc ttctccctgg
     5281 agaacgcact gtacgctctg tccgccgtgg gccactttac actgggctgc gtattggagg
     5341 aacaggagca tcaagtagca aaagaggaaa gagagacacc taccaccgat tctatgcccc
     5401 cacttctgag acaagcaatt gagctgttcg accggcaggg agccgaacct gccttccttt
     5461 tcggcctgga actaatcata tgtggcctgg agaaacagct aaagtgcgaa agcggcgggc
     5521 cggcctcgag cgaattccag tacctgccag atacagacga tcgtcaccgg attgaggaga
     5581 aacgtaaaag gacatatgag accttcaaga gcatcatgaa gaagagtcct ttcagcggac
     5641 ccaccgaccc ccggcctcca cctcgacgca ttgctgtgcc ttcccgcagc tcagcttctg
     5701 tccccaagcc agcaccccag ccctatccct ttacgtcatc cctgagcacc atcaactatg
     5761 atgagtttcc caccatggtg tttccttctg ggcagatcag ccaggcctcg gccttggccc
     5821 cggcccctcc ccaagtcctg ccccaggctc cagcccctgc ccctgctcca gccatggtat
     5881 cagctctggc ccaggcccca gcccctgtcc cagtcctagc cccaggccct cctcaggctg
     5941 tggccccacc tgcccccaag cccacccagg ctggggaagg aacgctgtca gaggccctgc
     6001 tgcagctgca gtttgatgat gaagacctgg gggccttgct tggcaacagc acagacccag
     6061 ctgtgttcac agacctggca tccgtcgaca actccgagtt tcagcagctg ctgaaccagg
     6121 gcatacctgt ggccccccac acaactgagc ccatgctgat ggagtaccct gaggctataa
     6181 ctcgcctagt gacaggggcc cagaggcccc ccgacccagc tcctgctcca ctgggggccc
     6241 cggggctccc caatggcctc ctttcaggag atgaagactt ctcctccatt gcggacatgg
     6301 acttctcagc cctgctgagt cagatcagct cctaagatca agcttatcga taatcaacct
     6361 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     6421 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     6481 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     6541 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     6601 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     6661 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     6721 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     6781 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     6841 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     6901 cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga
     6961 ggttcgcttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     7021 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatggg atcaattcac
     7081 catgggaata acttcgtata gcatacatta tacgaagtta tgctgctttt tgcttgtact
     7141 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     7201 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     7261 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     7321 agcatctagt tgaatcgcca gtgtgtcgag ctagcagatc tttttccctc tgccaaaaat
     7381 tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt
     7441 ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg
     7501 gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgccata
     7561 tgctggctgc catgaacaaa ggtggctata aagaggtcat cagtatatga aacagccccc
     7621 tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt tttttatatt
     7681 ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc
     7741 cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc tcttatgaag
     7801 atccctcgac ctgcagccca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     7861 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     7921 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     7981 agtcgggaaa cctgtcgtgc cagcggatcc gcatctcaat tagtcagcaa ccatagtccc
     8041 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     8101 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     8161 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc taacttgttt
     8221 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     8281 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     8341 tggatccgct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     8401 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     8461 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     8521 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     8581 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     8641 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     8701 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     8761 gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc
     8821 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     8881 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     8941 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     9001 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     9061 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     9121 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     9181 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     9241 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     9301 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     9361 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     9421 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     9481 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     9541 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     9601 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     9661 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     9721 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     9781 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     9841 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     9901 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     9961 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
    10021 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
    10081 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
    10141 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
    10201 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
    10261 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
    10321 gaaaagtgcc acctg
//
