LOCUS       sequence-215800-       13298 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tissue-specific expression of the GFP living color under the control
            of the Ngn3 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13298)
  TITLE     Salmon Lab plasmids
REFERENCE   2  (bases 1 to 13298)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCLX-Ngn3-GFP
FEATURES             Location/Qualifiers
     source          1..13298
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             385..642
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    689..814
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1306..1423
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    1783..2016
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2201..2245
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     protein_bind    2522..2542
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    8523..8547
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      8551..8560
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(8557..8559,8560..8562,8563..9276)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(8557..8559,8560..8562,8563..9273)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8602..8623)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8863..8882)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9210..9231
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(9278..9302)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    9320..9908
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9373..9393)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9791..9802)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(10051..10084)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             10104..10284
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10322..10341)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    10387..10442
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(10441..10460)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(10801..10817)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10801..10817)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10814..10836)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10825..10841
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10849..10855,10856..10873,10874..10879))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10894..10915
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        10974..11169
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      11020..11155
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    11175..11309
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11212..11231)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11266..11285
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11377..11394)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11548..12136)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11628..11647)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12307..13098,13099..13167))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12930..12949
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13168..13272)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggaggcg tggcctgggc gggactgggg agtggcgagc
      421 cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc
      481 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
      541 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
      601 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
      661 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
      721 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
      781 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
      841 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
      901 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
      961 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     1021 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     1081 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaaaaa
     1141 agcacagcaa gcagcaggat cttcagacct ggaaattccc tacaatcccc aaagtcaagg
     1201 agtagtagaa tctatgaata aagaattaaa gaaaattata ggacaggtaa gagatcaggc
     1261 tgaacatctt aagacagcag tacaaatggc agtattcatc cacaatttta aaagaaaagg
     1321 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     1381 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     1441 cagcagaaat ccactttgga aaggaccagc aaagctcctc tggaaaggtg aaggggcagt
     1501 agtaatacaa gataatagtg acataaaagt agtgccaaga agaaaagcaa agatcattag
     1561 ggattatgga aaacagatgg caggtgatga ttgtgtggca agtagacagg atgaggatta
     1621 gaacatggaa aagtttagta aaacaccata aggaggagat atgagggaca attggagaag
     1681 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1741 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1801 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1861 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1921 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1981 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     2041 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     2101 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     2161 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     2221 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     2281 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     2341 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     2401 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     2461 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgctagt gattatcgga
     2521 tcaactttgt atagaaaagt tggatctctc ctttaacaga ggagaaacag gcccagagga
     2581 aagatatgct ttcagtgaca caggagtagc cgcagagcca ggctgcctcc cccaccccac
     2641 ccccaatcct cacgcacatc tgctgctctt cccatcaagt tttgtcaccg aggctcgcat
     2701 ctggaaacgt gcatgcaacc tgctaaggag acaagcaggc tggagaagct gccaagcaga
     2761 atcctcccgg tgtacccgcc cgcccctagt gcgcctcagg tggacgccaa ctcctgggaa
     2821 tcccaggagg acgggagagt cttcaccatc cgagagttca agagcccagg ggtcaggcga
     2881 tgcccagcga gcacccccag actttccaac cctctccgct ccgtccagcg gcccctgtcc
     2941 cagccgcctt ttggcaagcc gcgctcttcc tccgctgccc tgcgctctcc tggctgccag
     3001 cactgtgagg tggcgcaacg cgccaaaggg caagagctcc aaactggagc cgcctggagg
     3061 tggcctcccc tcccccgccg ggttccgttg tttatttctt cctggggagg gaaaatcaca
     3121 cagcctattc gtttccccag ccgggatggg cgcagagccg gacatatggg catctcgcgc
     3181 tccgcatctg tgtcatcagt cgcgttttct gtggatgcct gcgtgtttgt acgtgtgtgt
     3241 gtatttgggg ccaagcgctg ggaacaaatg tgcatgtgtt aaatttgaca ataaagtcct
     3301 acagcgaagc tgctaagatt ggggcggtag gtgggggtgg aagacggggg gtggggggtg
     3361 gaggagagct aaactctcaa aaggcgaggc caaagcgcct ggcccaggta ttatgttggg
     3421 agaaggtgag gagagcagct gctccttctc gcggggtgtg tatgggagcg agggtgcatg
     3481 tgtccctgtg aagagtacac aacgatctgc tcaacatatg tcgccacaaa tcacagtgtc
     3541 tctccctcct cccacccccc gctccctgcg cggctcctga ccgagcgcag agcagagagg
     3601 ggcgctgaga agaggagccg cttcaacagg tgtcttggtg cgcaatgcac cttttcacgc
     3661 attgacggtc tcagccgccg gcgaggataa tgtggacact ttgtttccag gcgccgcgcg
     3721 cgagctgcgg acgcctaggg gaggcggccc tgagcaggga ggggggatct ggaaataaag
     3781 acctccggct ctcctgagcg cccgggccct ctcttccctt tgttcgaaga ctccctgggc
     3841 cggagctccc gtgtgcgccc tggggggtgg catatggcgc cctgtcacct acccaagaaa
     3901 aagaaccggc caccctttca agagcgcacg aggtccctga gcctggggag ggggagtctc
     3961 tgagggaacg gcttctctcc gaaagtgggt gaggagcccg tcttatctct ccctaccctg
     4021 cctccaccat cccctcagct ggcgcagcag ccgttgggtg gggagctgcg cgctgggcag
     4081 caccagcctg cggaagcgct ccagctcctg gcagatgaaa tatgggtctg gtgagcccct
     4141 ggagaccata tgctttcaat aaaagaagac aaataggacc gagataggcc ttgcagccaa
     4201 cctgttataa atgagtggca attgggccaa tgtacacggg ggcagggccc ccgcggggag
     4261 gcctgcttgg tgccaaatcc atgtgtcagc ttctgggaca ggtgtgtcca ggggccaggg
     4321 gccaggtctc ccccctccgg gtttatcacg gcaaaggtaa tattgtgcta actatgagta
     4381 aacagtcatt gtgaaaatga gggagagttt atcagggcga actagttggg gggtggctta
     4441 acaataaagt tgttcgcctt aggagcaggt gacggctggc tgcacggact cccgcggtag
     4501 atgttttcca aagcactcca ctttacaatc tcttgtaatt atttattaaa cgaaatctat
     4561 ttattattat tttagcaaac actggagaca ggtggggctt tcttttcgtc gtctcctttt
     4621 gtttgaaggg ctgtttgaag tggccagtct cggcccgggc agctcgctcg ccagattttt
     4681 gtcttcccct ctttcactgc tgagctgaag gcgagagaaa gaatccccct cctctggcaa
     4741 gccattagct attcagcttc ccatggcccc cctccctccc tgcacaattc tcaaagagcc
     4801 cccaggcaat gctaaaacta cccccgccga caagaaggca ggaaggcggg ccgctgcctt
     4861 ccttcttttc caatgtgccc gcggtctcca ggcgtctggc taccgcctct ctccgggacc
     4921 cctcagcctc caggcaaact taggtctcag gaggtgtagg ggtgcgggag tcctggaggt
     4981 cttaggggcg gtggatgacc aggagctgca ggaacagact ctgctgtctg cgggaagagc
     5041 catttcacaa acactgccgg tgcgcacgga gtcattaccg cctgcgcctc cgcagccaaa
     5101 ggcacaggaa ttaactttgg agaggggtct atgtgtcccc tgcagctgag acagagggca
     5161 tggatcctca atctctaatc gcctaagact ccttgacctc cggccctggc ctcctggctg
     5221 gaagaggact gcgggaaatc cttgcatccc tcaagaaggc ggctgccccc agccaccgcg
     5281 cgctcctcgc cctggctgct ttttgcgcaa agcaggagtg atggagaact gcaagccctg
     5341 gagggccctg agccggaggg gtttggggga ggctggataa gaattccaag caggtcccag
     5401 aattctctac ctatcaagaa agcttgaaag aatccccaga gtctttcctg gcatgggaag
     5461 ctcaccctgc gaaccttgtc agtatccatt agccgctcca ggcaggcccc agccagagtg
     5521 agacctaccc ctatctgagg tccactcacc cccatctgag gtccaccgcc agatgagagt
     5581 catccacagg cttctgggaa accagagcgg tgcttggatc cctcgtggcc agggttccct
     5641 ccaaggtgct tagccaggtc aggaggccct agagaagcat ggtttggatt ttctttccca
     5701 gaccaaaaaa gctccaagtt ggttctctcc cagtttctaa cttgcagtta aataaatcag
     5761 gcaaggctgg cctatgaggc agacaagtgt gaagaaggag aaggaggagg agaaggagaa
     5821 ggagaaagaa gaagaaggag gagaagaaga agaagaagaa gaagaggagg aggaggagga
     5881 ggaggaggag gaggaggaag cagcagcagc agcagcagct tgaatggaca gtggttcccc
     5941 ttgcctagaa aatgggacca ttatttcttt tctaatctga cccccagact caggacttcc
     6001 tctattttct gcattttggg gtctcttgtt ttgccttgaa aaaaaatgtt ttctcccaaa
     6061 tcaaggagca gtagctggtg caagggaaaa tctagggcta ggagtcttaa gatatgactt
     6121 ctatgtggtt ctgatagaac ttgctgtgtg accttgagag agtcactccc cctctctggg
     6181 ccttgatttt ttcatcttta aagaaggcct caaattccca ttcttatgag aagaagacaa
     6241 gctcctagtg agtggtgacc taagggagca gctgcagcaa aatgctaacc tgacagtccc
     6301 agatggtccc tttattggtt ctgaccctgg tctcaggctt catttcccca cagcaaggga
     6361 aggagcctgc tcacagagca ccagctaaga tcagcaggac cgcgccacac ccccgcccag
     6421 tcctagagcc cccctctcgc tggttcctga gcataccacc ctcttccttg gaggaaaatt
     6481 tgcccccaag cagcctaggc ggtaagaggc tatcactagg gcagactcac agacctacct
     6541 catcccctca ccccacccta cagtctcgaa gtcgggtcct gtcccctcct gcagtttccg
     6601 ggagactcag gatatctgga cctgctagaa agagaagcct tcctcgccta aggagactta
     6661 aaccgggata cttaaacctc ccgcctcggc gtcttcctcc aggcacgacc gggtcaagag
     6721 agagaagcgg aagctgcaac ccctcactct gagtgaccgg aagcagaaga ccacgggatg
     6781 tcccaggcgg ggacaagagg aggggctggg gaagaaagga gggatgatga gttcagagtc
     6841 cctttggaaa ggtttccaga gagcgctacc agggacaacc caaggggctg gggaagtccc
     6901 tgccttgtgc tctctgtgcg atgcccgagt gatgcagagg cagggggctg gagcaggtga
     6961 ctgctggcag ctgctgtctg tctgtgattg gaccggagga ctaaggggag aaaaagttta
     7021 tcagcttctc ccagtgcctg cacgctgtgg tagttcaaaa gacacgaggg ggaggggcac
     7081 agcagctctg cttcccagcg ccttgggaga ctgaagtgaa aggaacgctt gagcccagga
     7141 gttcgagacc atcctgggca acaaagcaag accgcccctc accccataca aaataaaaat
     7201 acaaataaat tagccgggca cagtggcgca tgcctgtagt ctcagctact gggaaggctg
     7261 aagtgggagg atagcttgag cccaggagat caaggctgca gtgagctgtg attgcaccac
     7321 tgcagtccag cctgggcgac agaaggagac cgttttttgg ttttgtttgt tcgtttaaaa
     7381 aaaaaaagaa gcaagagctc actgtgaact cctggttcct tcctcccctc ctcacacttc
     7441 ccagaactct tcctgtcacg gttcctggcc agaacgctgg gatactatct acaagctgta
     7501 gtaggcttgt agtaatggaa tgtccgcttg aggggtcccc gcacagccaa cccctgcctc
     7561 tggagtggga tctatggggg tggggttcta agcgcctctg gggagtgtga ggtagcatct
     7621 cagggtgtgg cagaggctcg gacaccccca aaaggtctgc gaatggaagg gacataggca
     7681 ggatctctct cagtgatgtc ccctgtcttc caggatgaag agaggcagtg aaacaccagg
     7741 agagcagggc gtcctttaga attcctggac ccttctccag gctgctagtc aggacaatga
     7801 gctcgtggtt gtctttgcca ctatcttcct gtgcgatttc agacaagcca cctccctcac
     7861 taagcctaaa tttccccatg tgtaacgtgc aggcattgta ccctagaggc atcaaagtcc
     7921 cctccaggac agatgctaag gaaagatagg ctaggagcaa agccgtctga ggtggcctga
     7981 ccagagccac acgaggctct tctcactggg cgaggctctt tgaggaaccg agagttgctg
     8041 ggacccagcc cgccctcgag agagcaaaca gagcggcgct cccctccccc gaccccggcc
     8101 ctttgtccgg aatccagctg tgccctgcgg gggaggagcg ggctcgcgtg gcgcggcccc
     8161 agggccccgg cgctgattgg ccggtggcgc gggcagcagc cgggcaggca cgctcctggc
     8221 ccgggcgaag cagataaagc gtgccaaggg gcacacgact tgctgctcag gaaatccctg
     8281 cggtctcacc gccgcgcctc gagagagagc gtgacagagg cctcggaccc cattctctct
     8341 tcttttctcc tttggggctg gggcaactcc caggcggggg cgcctgcagc tcagctgaac
     8401 ttggcgacca gaagcccgct gagctcccca cggccctcgc tgctcatcgc tctctattct
     8461 tttgcgccgg tagaaaggta atatttggag gcctccgagg gacgggcagg ggaaagaggg
     8521 atccaacttt gtacaaaaaa gcaggctccc gccaccatgg tgagcaaggg cgaggagctg
     8581 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     8641 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     8701 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
     8761 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     8821 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     8881 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     8941 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     9001 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
     9061 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     9121 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg
     9181 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     9241 gggatcactc tcggcatgga cgagctgtac aagtaagacc cagctttctt gtacaaagtg
     9301 gtgataatcg aattccgata atcaacctct ggattacaaa atttgtgaaa gattgactgg
     9361 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     9421 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     9481 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     9541 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     9601 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     9661 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac
     9721 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     9781 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     9841 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     9901 ctccccgcat cgggaattcc cgcggttcgc tttaagacca atgacttaca aggcagctgt
     9961 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
    10021 aagacaagat gggatcaatt caccatggga ataacttcgt atagcataca ttatacgaag
    10081 ttatgctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
    10141 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
    10201 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
    10261 agtcagtgtg gaaaatctct agcagcatct agttgaatcg ccagtgtgtc gagctagcag
    10321 atctttttcc ctctgccaaa aattatgggg acatcatgaa gccccttgag catctgactt
    10381 ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct
    10441 cactcggaag gacatatggg agggcaaatc atttaaaaca tcagaatgag tatttggttt
    10501 agagtttggc aacatatgcc atatgctggc tgccatgaac aaaggtggct ataaagaggt
    10561 catcagtata tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact
    10621 tgaggttaga ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa
    10681 ttttccttac atgttttact agccagattt ttcctcctct cctgactact cccagtcata
    10741 gctgtccctc ttctcttatg aagatccctc gacctgcagc ccaagcttgg cgtaatcatg
    10801 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
    10861 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
    10921 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagcgga tccgcatctc
    10981 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
    11041 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
    11101 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
    11161 ttttgcaaaa agctaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
    11221 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
    11281 tcatcaatgt atcttatcat gtctggatcc gctgcattaa tgaatcggcc aacgcgcggg
    11341 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
    11401 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
    11461 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
    11521 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
    11581 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
    11641 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
    11701 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta
    11761 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
    11821 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
    11881 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
    11941 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
    12001 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
    12061 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
    12121 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
    12181 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
    12241 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
    12301 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
    12361 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
    12421 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
    12481 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
    12541 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
    12601 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
    12661 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
    12721 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
    12781 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
    12841 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
    12901 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
    12961 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
    13021 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
    13081 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
    13141 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
    13201 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
    13261 aggggttccg cgcacatttc cccgaaaagt gccacctg
//
