LOCUS       sequence-215802-       10158 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tissue-specific expression of the tdTomato living color under the 
            control of the Rat Insulin 2 promoter (RIP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10158)
  TITLE     Salmon Lab plasmids
REFERENCE   2  (bases 1 to 10158)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p2K7-RIP-Tomato-EFs-Tk::Sh
FEATURES             Location/Qualifiers
     source          1..10158
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1803..1920
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1938..1958
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    2652..2676
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2680..2689
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2686..4116
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     protein_bind    complement(4118..4142)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4160..4748
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4213..4233)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4631..4642)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             4772..4813
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     promoter        4846..5057
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             6679..6912
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6984..7105
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7021..7040)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7075..7094
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7145..7280
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7300..7319)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7301..7319)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7329..7346)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7329..7345)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7338..7360)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7487..7942
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7574..7593)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7784..7805
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7968..8072
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8073..8141,8142..8933)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8291..8310)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9104..9692
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9593..9612
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9846..9863
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9980..10001
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10016..10021,10022..10039,10040..10046)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10054..10070
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10059..10081
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10078..10094
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10078..10094
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10113..10133
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10115..10133
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttgac cactccagtg gaggctgaga
     1981 aaggttttgt agctgggtag agtatgtact aagagatgga gacggctggc tctgagctct
     2041 gaagcaagca cctcttatgg agagttgctg accttcaggt gcaaatctaa gatactacag
     2101 gagaatacac catggggctt cagcccagtt gactcccgag tgggctatgg gtttgtggaa
     2161 ggagagatag aagagaaggg acctttcttc ttgaattctg ctttccttct acctctgagg
     2221 gtgagctggg gtctcagctg aggtgaggac acagctatca gtgggaactg tgaaacaaca
     2281 gttcaaggga caaagttact aggtccccca acaactgcag cctcctgggg aatgatgtgg
     2341 aaaaatgctc agccaaggac aaagaaggcc tcaccctctc tgagacaatg tcccctgctg
     2401 tgaactggtt catcaggcca cccaggagcc cctattaaga ctctaattac cctaaggcta
     2461 agtagaggtg ttgttgtcca atgagcactt tctgcagacc tagcaccagg caagtgtttg
     2521 gaaactgcag cttcagcccc tctggccatc tgctgatcca cccttaatgg gacaaacagc
     2581 aaagtccagg ggtcaggggg ggggtgcttt ggactataaa gctagtgggg attcagtaac
     2641 ccccagccct aacaactttg tacaaaaaag caggctcccg ccaccatggt gagcaagggc
     2701 gaggaggtca tcaaagagtt catgcgcttc aaggtgcgca tggagggctc catgaacggc
     2761 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
     2821 aagctgaagg tgaccaaggg cggccccctg cccttcgcct gggacatcct gtccccccag
     2881 ttcatgtacg gctccaaggc gtacgtgaag caccccgccg acatccccga ttacaagaag
     2941 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggtctg
     3001 gtgaccgtga cccaggactc ctccctgcag gacggcacgc tgatctacaa ggtgaagatg
     3061 cgcggcacca acttcccccc cgacggcccc gtaatgcaga agaagaccat gggctgggag
     3121 gcctccaccg agcgcctgta cccccgcgac ggcgtgctga agggcgagat ccaccaggcc
     3181 ctgaagctga aggacggcgg ccactacctg gtggagttca agaccatcta catggccaag
     3241 aagcccgtgc aactgcccgg ctactactac gtggacacca agctggacat cacctcccac
     3301 aacgaggact acaccatcgt ggaacagtac gagcgctccg agggccgcca ccacctgttc
     3361 ctggggcatg gcaccggcag caccggcagc ggcagctccg gcaccgcctc ctccgaggac
     3421 aacaacatgg ccgtcatcaa agagttcatg cgcttcaagg tgcgcatgga gggctccatg
     3481 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
     3541 accgccaagc tgaaggtgac caagggcggc cccctgccct tcgcctggga catcctgtcc
     3601 ccccagttca tgtacggctc caaggcgtac gtgaagcacc ccgccgacat ccccgattac
     3661 aagaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
     3721 ggtctggtga ccgtgaccca ggactcctcc ctgcaggacg gcacgctgat ctacaaggtg
     3781 aagatgcgcg gcaccaactt cccccccgac ggccccgtaa tgcagaagaa gaccatgggc
     3841 tgggaggcct ccaccgagcg cctgtacccc cgcgacggcg tgctgaaggg cgagatccac
     3901 caggccctga agctgaagga cggcggccac tacctggtgg agttcaagac catctacatg
     3961 gccaagaagc ccgtgcaact gcccggctac tactacgtgg acaccaagct ggacatcacc
     4021 tcccacaacg aggactacac catcgtggaa cagtacgagc gctccgaggg ccgccaccac
     4081 ctgttcctgt acggcatgga cgagctgtac aagtaagacc cagctttctt gtacaaagtg
     4141 gtgataatcg aattccgata atcaacctct ggattacaaa atttgtgaaa gattgactgg
     4201 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     4261 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     4321 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     4381 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     4441 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     4501 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac
     4561 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     4621 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     4681 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     4741 ctccccgcat cgggaattcc cgcggttcga aggtaagcct atccctaacc ctctcctcgg
     4801 tctcgattct acgcgtaccg ggcgatggct ccggtgcccg tcagtgggca gagcgcacat
     4861 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaaccggt gcctagagaa
     4921 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg
     4981 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt
     5041 ttgccgccag aacacaggtg tcgtgacgcg gatccaggcc tacaccatgg cttcttaccc
     5101 tggacaccag catgcttctg cctttgacca ggctgccaga tccaggggcc actccaacag
     5161 gagaactgcc ctaagaccca gaagacagca ggaagccact gaggtgaggc ctgagcagaa
     5221 gatgccaacc ctgctgaggg tgtacattga tggacctcat ggcatgggca agaccaccac
     5281 cactcaactg ctggtggcac tgggctccag ggatgacatt gtgtatgtgc ctgagccaat
     5341 gacctactgg agagtgctag gagcctctga gaccattgcc aacatctaca ccacccagca
     5401 caggctggac cagggagaaa tctctgctgg agatgctgct gtggtgatga cctctgccca
     5461 gatcacaatg ggaatgccct atgctgtgac tgatgctgtt ctggctcctc acattggagg
     5521 agaggctggc tcttctcatg cccctccacc tgccctgacc ctgatctttg acagacaccc
     5581 cattgcagcc ctgctgtgct acccagcagc aaggtacctc atgggctcca tgaccccaca
     5641 ggctgtgctg gcttttgtgg ccctgatccc tccaaccctc cctggcacca acattgttct
     5701 gggagcactg cctgaagaca gacacattga caggctggca aagaggcaga gacctggaga
     5761 gagactggac ctggccatgc tggctgcaat cagaagggtg tatggactgc tggcaaacac
     5821 tgtgagatac ctccagtgtg gaggctcttg gagagaggac tggggacagc tctctggaac
     5881 agcagtgccc cctcaaggag ctgagcccca gtccaatgct ggtccaagac cccacattgg
     5941 ggacaccctg ttcaccctgt tcagagcccc tgagctgctg gctcccaatg gagacctgta
     6001 caatgtgttt gcctgggctc tggatgttct agccaagagg ctgaggtcca tgcatgtgtt
     6061 catcctggac tatgaccagt cccctgctgg atgcagagat gctctgctgc aactaacctc
     6121 tggcatggtg cagacccatg tgaccacccc tggcagcatc cccaccatct gtgacctagc
     6181 cagaaccttt gccagggaga tgggaatctc tggagccaat ggagtcatgg ccaagttgac
     6241 cagtgctgtc ccagtgctca cagccaggga tgtggctgga gctgttgagt tctggactga
     6301 caggttgggg ttctccagag attttgtgga ggatgacttt gcaggtgtgg tcagagatga
     6361 tgtcaccctg ttcatctcag cagtccagga ccaggtggtg cctgacaaca ccctggcttg
     6421 ggtgtgggtg agaggactgg atgagctgta tgctgagtgg agtgaggtgg tctccaccaa
     6481 cttcagggat gccagtggcc ctgccatgac agagattgga gagcagccct gggggagaga
     6541 gtttgccctg agagacccag caggcaactg tgtgcacttt gtggcagagg agcaggactg
     6601 aggataagaa ttctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     6661 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     6721 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     6781 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     6841 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     6901 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     6961 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
     7021 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     7081 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc
     7141 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     7201 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     7261 aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac
     7321 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     7381 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     7441 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc
     7501 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     7561 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     7621 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     7681 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     7741 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     7801 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     7861 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     7921 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     7981 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     8041 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     8101 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     8161 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     8221 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     8281 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     8341 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     8401 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     8461 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     8521 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     8581 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     8641 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     8701 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     8761 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     8821 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     8881 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     8941 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     9001 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     9061 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     9121 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     9181 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     9241 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     9301 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     9361 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     9421 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     9481 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     9541 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     9601 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     9661 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     9721 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     9781 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     9841 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     9901 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     9961 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
    10021 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
    10081 gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa
    10141 agctggagct gcaagctt
//
