LOCUS       sequence-215803-        8824 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tissue-specific expression of the GFP living color under the control
            of the Keratin7 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8824)
  TITLE     Salmon Lab plasmids
REFERENCE   2  (bases 1 to 8824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCLX-KRT7-GFP
FEATURES             Location/Qualifiers
     source          1..8824
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             385..642
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    689..814
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1306..1423
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    1783..2016
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2201..2245
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     protein_bind    2522..2542
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    4049..4073
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      4077..4086
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4083..4085,4086..4088,4089..4802)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4083..4085,4086..4088,4089..4799)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4128..4149)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4389..4408)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4736..4757
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(4804..4828)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4846..5434
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4899..4919)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5317..5328)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(5577..5610)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             5630..5810
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5848..5867)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5913..5968
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5967..5986)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6327..6343)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6327..6343)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6340..6362)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6351..6367
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6375..6381,6382..6399,6400..6405))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6420..6441
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6500..6695
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6546..6681
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6701..6835
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6738..6757)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6792..6811
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6903..6920)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7074..7662)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7154..7173)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7833..8624,8625..8693))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8456..8475
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8694..8798)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggaggcg tggcctgggc gggactgggg agtggcgagc
      421 cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc
      481 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
      541 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
      601 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
      661 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
      721 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
      781 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
      841 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
      901 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
      961 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     1021 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     1081 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaaaaa
     1141 agcacagcaa gcagcaggat cttcagacct ggaaattccc tacaatcccc aaagtcaagg
     1201 agtagtagaa tctatgaata aagaattaaa gaaaattata ggacaggtaa gagatcaggc
     1261 tgaacatctt aagacagcag tacaaatggc agtattcatc cacaatttta aaagaaaagg
     1321 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     1381 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     1441 cagcagaaat ccactttgga aaggaccagc aaagctcctc tggaaaggtg aaggggcagt
     1501 agtaatacaa gataatagtg acataaaagt agtgccaaga agaaaagcaa agatcattag
     1561 ggattatgga aaacagatgg caggtgatga ttgtgtggca agtagacagg atgaggatta
     1621 gaacatggaa aagtttagta aaacaccata aggaggagat atgagggaca attggagaag
     1681 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1741 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1801 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1861 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1921 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1981 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     2041 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     2101 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     2161 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     2221 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     2281 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     2341 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     2401 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     2461 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgctagt gattatcgga
     2521 tcaactttgt atagaaaagt tggctagcac tggagtttta aagaatgttc cagggagggg
     2581 taagaggccc agggcagatg tggggtaggc tcaggaatga ggaagtattt ctcaagcctg
     2641 tgtgtggtgg ttataggggg ccgaggcctg gactgaagtg cccaggtact tttgtctgtg
     2701 tgcttcccag ctcttgttag accctcatcc tgaaggagct gtggctgagg gggccaggac
     2761 ccgtccaggt cagcgcacta gctgtgccct gctggggctc agcttgagaa agagaaggag
     2821 ctcttggtgt gggtgagaaa caacctccaa agtgggggat gggtggcagg ggctgtagca
     2881 ggatggaaag tggggtgcag agggggacaa aggactgagg aagcaccttg aggggtaggg
     2941 agtctccttg agagcttagg gttgtctacc tgaaaaggca aagcaggaag aatatgacat
     3001 tcccatcctc cccacctagg acgcccctaa aggaccagtc tcactgcatg gagccagagg
     3061 tgggctaaag gctgtccaag tgagtctggg aaaactggaa gtccaggagc actccgagga
     3121 gcactgagca caccaccacg ggttaccaca gaggccagtt ttagttgatt cccttgtcag
     3181 gcatagccta gtgggtttgt aagcaggaaa aatgaggcag agcagggagg aacgtctgga
     3241 tgtgaacggg agacaggaca cacacatgtg catacaaatg tgttcagttg tgaatccaga
     3301 ccccccatgc acagactgca ctcatataca ctcacactag tgtgttaggg gtatgagggc
     3361 acagaagggt acgcacagcc atcccaggaa cccatacagt agtttgagga gggggtgagg
     3421 caagccctct gaacttggat ggtggggagg gcgcagttac tgaaacagct cttcccagta
     3481 aggatcgtca cagggccagg ttactcaaga actgagctga gctctccagt agcagctggg
     3541 ccaggaagga gagtcctagg aagcctaggg cttccccagc cagtgggtga ggggtttcaa
     3601 ctttcagcag gagctaaagt ttgtcaggca cttccttttg tagcacttgg ctttcctact
     3661 ggcctcagga atctaggtat tcactcctca tacctgctct aggaagcctt ctcctgccaa
     3721 ttgtaaggtg gtctctttgc ggtccagaga tttctgggct gtgggtatct tggacaactt
     3781 gcctgaggtg tgggtgtctc acgacggcgt ctcggcctcc tgcagacact tcccgagtgg
     3841 acgaagagcc ttgggcggcg gctgggggcg gtgcgagggc agaaggcagg cggacaggaa
     3901 ggccctggct gcagagaaag gtggccagcg gccagccccg ccctgtcccc acctgtgtgc
     3961 gctcagcctc cgccccgcat ggaggaataa aaggtcgcag actgcctgag gtcagggagt
     4021 tcgcgctgct cctcgcctcg tccagccagc aactttgtac aaaaaagcag gctcccgcca
     4081 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     4141 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     4201 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     4261 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
     4321 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     4381 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     4441 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     4501 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     4561 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     4621 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     4681 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     4741 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt
     4801 aagacccagc tttcttgtac aaagtggtga taatcgaatt ccgataatca acctctggat
     4861 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     4921 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     4981 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     5041 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     5101 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     5161 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     5221 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc
     5281 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     5341 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     5401 acgagtcgga tctccctttg ggccgcctcc ccgcatcggg aattcccgcg gttcgcttta
     5461 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     5521 ctggaagggc taattcactc ccaacgaaga caagatggga tcaattcacc atgggaataa
     5581 cttcgtatag catacattat acgaagttat gctgcttttt gcttgtactg ggtctctctg
     5641 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     5701 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     5761 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gcatctagtt
     5821 gaatcgccag tgtgtcgagc tagcagatct ttttccctct gccaaaaatt atggggacat
     5881 catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat
     5941 agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt
     6001 aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgccatat gctggctgcc
     6061 atgaacaaag gtggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt
     6121 ccttattcca tagaaaagcc ttgacttgag gttagatttt ttttatattt tgttttgtgt
     6181 tatttttttc tttaacatcc ctaaaatttt ccttacatgt tttactagcc agatttttcc
     6241 tcctctcctg actactccca gtcatagctg tccctcttct cttatgaaga tccctcgacc
     6301 tgcagcccaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     6361 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     6421 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     6481 ctgtcgtgcc agcggatccg catctcaatt agtcagcaac catagtcccg cccctaactc
     6541 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     6601 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     6661 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct aacttgttta ttgcagctta
     6721 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     6781 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatccgctg
     6841 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     6901 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     6961 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     7021 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     7081 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     7141 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     7201 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     7261 ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     7321 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     7381 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     7441 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     7501 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     7561 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     7621 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     7681 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     7741 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     7801 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     7861 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     7921 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     7981 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     8041 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     8101 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     8161 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     8221 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     8281 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     8341 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     8401 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     8461 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     8521 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     8581 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     8641 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     8701 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     8761 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     8821 cctg
//
